ITS와 psbA-trnH 염기서열에 의한 한국산 메꽃속(Calystegia R.Br.)의 분류학적 연구

Molecular Phylogenetic Studies of Korean Calystegia R.Br. Based on ITS and psbA-trnH Sequences

  • 김상준 (영남대학교 이과대학 생명과학과) ;
  • 박선주 (영남대학교 이과대학 생명과학과)
  • Kim, SangJun (Department of Life Science, Yeungnam University) ;
  • Park, SeonJoo (Department of Life Science, Yeungnam University)
  • 투고 : 2011.11.21
  • 심사 : 2011.12.09
  • 발행 : 2011.12.31

초록

한국산 메꽃속 4종 1변종과 1종의 외군을 대상으로 종의 실체를 확인하고, 유연관계 및 진화경향성을 파악하기 위하여 분자계통학적 연구를 수행하였다. 메꽃속의 주요 형질로는 잎의 모양과 화관의 길이, 털의 유무 등이 있다. 하지만 잎의 형질에서 많은 변이 현상이 나타나고 있어 종 분류에 어려움이 있다. 분자계통학적 연구결과 갯메꽃이 하나의 분계조를 형성하여 가장 기부에 위치하였고, 애기메꽃과 큰메꽃의 ITS 지역과 psbA-trnH 지역에서는 독립적인 분계조를 형성하지 못하여 두 종의 관계가 명확하지 않았다. 따라서 두 종의 관계는 본 연구에 사용된 마커들로는 부족하며 앞으로 더 많은 개체와 다양한 마커들을 통한 연구가 수반되어야 한다. 메꽃은 선메꽃과 유집되어 유연관계가 가까운 것으로 나타났다.

Molecular phylogenetic studies were conducted to evaluate evolutionary trends, relationships and species identities among four species, one variety and one outgroup of the Korean Genus Calystegia. The important characteristics of Calystegia are the shape of the lamina, the length ofthe corolla and the presence of hair. However, many variations were observed as regards the characteristics of the leaf, making true identification difficult. In molecular phylogenetic studies, C. soldanella formed one clade, and it was located mostly in the base. C. hederacea and C. sepium did not form an independent clade in their ITS regions and psbA-trnH regions, and this investigation could not confirm a relationship. Therefore, a relationship between these two species is not sufficiently supported by these markers (ITS and psbA-trnH). Consequently, this research should be achieved through many samples and markers. C. sepium var. japonica and C. dahurica are closely related.

키워드

참고문헌

  1. Alvarez, I. and J. F. Wendel. 2003. Ribosomal ITS sequences and plant phylogenetic inference. Mol. Phylogen. Evol. 29: 417-434. https://doi.org/10.1016/S1055-7903(03)00208-2
  2. Baldwin, B. G. 1992. Phylogenetic utility of the internal transcribed spacers of nuclear ribosomal DNA in plants : An example from the Compositae. Mol. Phylogen. Evol. 1: 3-16. https://doi.org/10.1016/1055-7903(92)90030-K
  3. Choi, B. H. 2007. Convolvulaceae. In The genera of vascular plants of Korea. Flora of Korea Editorial Committee (eds.). Academy Publishing Co, Seoul. Pp. 794-796.
  4. Choisy, J. D. D. 1845. Convolvulaceae. In Prodromus Systematis Nauralis Regni Vegetabilis. 9: 433.
  5. Chung, M. G., S. T. Kim, H. G. Chung and M. S. Chung. 1995. Allozyme Diversity in Korean Populations of Calystegia soldanella and C. japonica (Convolvulaceae). J. Pl. Biol. 38(2): 173-180.
  6. Curtis, S. and Clegg, M. T. 1984. Molecular evolution of chloroplast DNA sequences. Mol. Biol. Evol. 1: 291-301.
  7. Drummond, A. J., B. Ashton, S. Buxton, M. Cheung, A. Cooper, C. Duran, M. Field, J. Heled, M. Kearse, S. Markowitz, R. Moir, S. Stones-Havas, S. Sturrock, T. Thierer, A. Wilson. 2011. Geneious v5.4, Available from http://www.geneious.com/.
  8. Farris J. S., Albert V. A., KLLersjo M., Lipscomb D. and Kluge A. G. 1996. Parsimony jackknifing outperforms neighbor-joining. Cladistics 12: 99-124. https://doi.org/10.1111/j.1096-0031.1996.tb00196.x
  9. Felsenstein J. 1985. Confidence limits on phylogenies : an approach using the bootstrap. Evolution 39: 783-791. https://doi.org/10.2307/2408678
  10. Kim, K. J. and R. K. Jansen. 1994. Comparisons of phylogenetic hypotheses among the different data sets in dwarf dadelion (Krigia, Asteraceae) : Additional information from internal transcribed spacer sequences of nuclear ribosomal DNA. Pl. Syst. Evol. 190: 157-185. https://doi.org/10.1007/BF00986191
  11. Kim, K. J., Y. D. Kim, J. H. Kim, S. J. Park, C. W. Park, B. Y. Sun, K. O. Yoo, B. H. Choi and S. T. Kim. 2008. Phylogenetic classification of Korean vascular flora according to the recent APG classification system. Korean J. Pl. Taxon. 38: 197-222 (in Korean).
  12. Kim, S. T. and M. G. Chung. 1995. Genetic and clonal structure in Korean populations of Calystegia japonica (Convolvulaceae). Bot. Bull. Acad. Sin. 36: 135-141.
  13. Kim, Y. D., C. W. Park., B. Y. Sun, K. J. Kim, E. J. Lee and S. H. Kim. 2005. ITS sequence variations in common ragweed and giant ragweed. Korean J. Pl. Taxon. 35: 273-285 (in Korean).
  14. Kim, Y. S. and B. H. Choi. 1983. Chromosome number, morphological and anatomical study on Calystegia in Korea. Korean J. Pl. Taxon. 13: 89-101 (in Korean).
  15. Kim, Y. S. and B. H. Choi. 1984. A numerical taxonomic study of Calystegia in Korea by the cluster analysis and principal component analysis. Korean J. Pl. Taxon 27: 33-41 (in Korean).
  16. Kimura, M. 1980. A simple method for estimating evolutionary rates of base substitution through comparative studies of nucleotide sequences. J. Mol. Evol. 16: 111-120. https://doi.org/10.1007/BF01731581
  17. Komarov, V. L. 1907. Flora Manshuriae. vol. III. Pp. 303-308.
  18. Lookerman, D. J. and R. K. Jansen. 1996. The use of herbarium material for DNA studies. In Sampling the green world. Stussey, T. J. and S. Sohmer (eds.), Columbia university Press, New York. Pp. 205-220.
  19. Makino, T. 1912. Index Plantarum Japonicarum Sive Enumeratio Plantarum Omnium. 2(2): 516.
  20. Nakai, T. 1911. Calystegia. In Flora Koreana. Pars secunda. J. Coll. Sci. Imp. Univ. Tokyo. 31: 108-110.
  21. Nakai, T. 1952. Calystegia. In A Synoptical Sketch of Korean Flora. Bull. Natl. Sci. Mus. 31: 94-95.
  22. Oh, Y. C., C. S. Lee., and E. J. Park. 1995. A chemotaxonomic study on the genus Calystegia (Convolvulaceae) in Korea. Korean J. Pl. Taxon. 25: 13-24 (in Korean).
  23. Ohwi, J. 1965. Calystegia. In Flora of Japan. Smithsonian Institution, Washington, D.C. Pp. 752-753.
  24. Palmer, J. 1900. Conspectus florae. Pars. secunda. trudy Imo. S. -Peterburgsk. Bot. Sada. 18: 147-198.
  25. Park, M. K. 1949. An Enumeration of Korean Plants. Seoul, Korea. p. 197.
  26. Pamer, J. D., B. Osorio and W. F. Thompson. 1988. Evolution significance of inversions in legume chloroplast DNAs. Curr. Genet. 14: 65-74. https://doi.org/10.1007/BF00405856
  27. Posada, A. D. and K. A. Crandall. 1998. Modeltest: Testing the model of DNA substitution. Bioinformatics 14: 817-818. https://doi.org/10.1093/bioinformatics/14.9.817
  28. Roemer, J. J. and J. J. A. Schultes. 1819. Calystegia. Systema vegetabilium. 4: 184.
  29. Sang, T., D. J. Crawford and T. F. Stuessy. 1997. Chloroplast DNA phylogeny, reticular evolution, and biogeography of Paeonia (Paeoniaceae). Amer. J. Bot. 84: 1120-1136. https://doi.org/10.2307/2446155
  30. Saitou, N. and M. Nei. 1987. The neighbor-joining method: A new for reconstructing Phylogenetic trees. Molec. Biol. Evol. 4: 406-425.
  31. Soltis, P. D., J. J. doyle and D. E. Soltis. 1992. Molecular data and polyploid evolution in plants. In: Molecular Systematics of Plant. Soltis, P. D., J. J. doyle and D. E. Soltis. (eds.), Chapman & Hall. New York. Pp. 177-201.
  32. Son, S. W., J. H. Kim, K. J. Kim and S. J. Park. 2009. Molecular evidence for the hybridity of Ilex ${\times}$ wandoensis and the phylogenetic study of Korean Ilex based on ITS sequence data. Gene & Genomics 31(1): 53-63. https://doi.org/10.1007/BF03191138
  33. Swofford, D. L. 2003. PAUP*. Phylogenetic Analysis Using Parsimony (*and Other Methods). Version 4. Sinauer Associates, Sunderland, Massachusetts.
  34. Wallich, N. 1824. Calystegia. In Flora Indica; or descriptions of Indian Plants. 2: 94-95.
  35. White, T. J., T. Birns, S. Lee and J. Taylor. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR protocols : A guide to methods and applications. D. G. M. Innis, J. Sninsky, and T. White (eds.), Academic Press, San Diego, C. A. Pp. 315-322.
  36. Willis, J. C. 1973. A Dictionary of the Flowering Plant and Ferns. 8th ed. Cambridge Univ. Press, London.