• 대한의생명과학회지
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• 제28권3호
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• pp.206-210
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• 2022

Calystegia dahurica (Herb.) Choisy is a natural product that has not been studied for efficacy or active ingredients. The purpose of this study is to investigate the activation effect of natural killer cells using a natural extract composition based on Calystegia dahurica (Herb.) Choisy extract (CDCE). We evaluated the activity of natural killer cells in natural products using PBMCs from healthy participants. All natural products were extracted with 50% ethanol. Based on the results of the cell viability assay, PBMCs of healthy participants were treated with extracts at various concentrations. Then, analysis was performed using flow cytometry to measure the cd107a surface expression of natural killer cells. As a result, treatment with a single extract of PBMCs increased the expression of cd107a in a concentration-dependent. Furthermore, it was confirmed that the treatment of the extract composition showed the highest expression of cd107a. In conclusion, it is expected that the extract composition containing CDCE according to this study can be used for prevention or treatment of cancer cells, tumor cells, and immune diseases.

• 식물분류학회지
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• 제41권4호
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• pp.338-344
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• 2011

한국산 메꽃속 4종 1변종과 1종의 외군을 대상으로 종의 실체를 확인하고, 유연관계 및 진화경향성을 파악하기 위하여 분자계통학적 연구를 수행하였다. 메꽃속의 주요 형질로는 잎의 모양과 화관의 길이, 털의 유무 등이 있다. 하지만 잎의 형질에서 많은 변이 현상이 나타나고 있어 종 분류에 어려움이 있다. 분자계통학적 연구결과 갯메꽃이 하나의 분계조를 형성하여 가장 기부에 위치하였고, 애기메꽃과 큰메꽃의 ITS 지역과 psbA-trnH 지역에서는 독립적인 분계조를 형성하지 못하여 두 종의 관계가 명확하지 않았다. 따라서 두 종의 관계는 본 연구에 사용된 마커들로는 부족하며 앞으로 더 많은 개체와 다양한 마커들을 통한 연구가 수반되어야 한다. 메꽃은 선메꽃과 유집되어 유연관계가 가까운 것으로 나타났다.

• Journal of Plant Biology
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• 제27권1호
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• pp.33-41
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• 1984

The relationships and character variations on 5 taxa of Calystegia were examined by sluster analysis and principal component analysis. Thirteen Calystegia population samples from the middle part of Korea were observed. Although minor differences were noted, essentially similar results were obtained from the phenograms by UPGMA, UPGMC and Ward's clustering methods, and these results were in accordance with those obtained from the ordination plots by principal component analysis. C. soldanella is distantly connected with the other taxa mainly because of its morphologically different leaf organs. Based on the difference on the first principal component, C. hederacae is kept apart from the rest 3 taxa. In the relationships among C. japonica, C. sepium var. americana and C. davurica, mivor differences were obtained from the 3 clustering methods. As to the character variations among different populations within a taxon, they are slight in C. soldanella and C. sepium var. americana, but remarkable in C. hederacae and C. davurica.

• 한국환경과학회지
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• 제22권2호
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• pp.187-193
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• 2013

Dry matter allocation characteristics of Calystegia soldanella, grown in pots, was analysed to assess its plasticity in response to water-stressed conditions. As water was withheld leaf water potential between the two watering treatments was similar during the first 6 days, followed by a rapid decrease in water-stressed plants. The minimum leaf water potential was -1.50 MPa on day 15 and the maximum leaf water potential was about -0.5 MPa on day 0 in water-stressed plants. In well-watered plants leaf water potential was maintained almost consistently throughout the experiment. There was no significant difference in plant dry weight between the two watering treatments for 9 days after the start of experiment and that was remarkably increased thereafter, compared with that remained without any increase in water-stressed plants. In dry mass partitioning, however, the water-stressed plants showed a great plasticity, showing that there were 1.81, 1.35 and 0.81 times increase in root, stem and leaf, respectively. Dry mass partitioning in well-watered plants varied from 2% to 5%. The difference of dry mass partitioning between the two watering treatments was reflected in leaf mass per unit area (LMA) and root/shoot (R/S) ratio. LMA in water-stressed plants was lower than that in well-watered plants, while R/S ratio in water-stressed plants was higher in well-watered plants. This means that the water-stressed plants reduced its leaf area and increased dry mass partitioning into root and stem during the progress of soil drying. These results indicate that Calystegia soldanella inhabiting in sand dune cope with water stress with high plasticity which can adjust its dry mass partitioning according to soil water conditions.

• Journal of Plant Biology
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• 제38권2호
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• pp.173-180
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• 1995

We investigated levels and distribution of genetic variation in Korean populations of Calystegia soldanella and C. japonica, clonally reproducing herbaceous perennials. Calystegia soldanella is one ofecologically important beach plants growing only on sand and beach dunes in Europe, East Asia, the Pacific Islands, and the west coast of North America. In contrast, C. japonica usually grows on small mounds of paddy fields, roadsides, and waste places with patchy distribution. Starch gel electrophoresis was conducted on leaves collected from 13 populations of C. soldanella and eight populations of C. japonica. The levels of genetic variation of the two species are very comparable; means of expected heterozygosity (Hep) were 0.100 and 0.099 for C. soldanella and C. japonica, respectively. These values were also very similar to those for species with similar life-history and ecological traits. However, the proportion of total genetic diversity partitioned among populations (GST) of C. soldanella (0.146) was considerably lower than that of C. japonica (0.383). In addition, means of Nei's genetic identity (Ⅰ) for C. soldanella and C. japonica were 0.985 and 0.900, respectively, which supports a restricted gene flow resulting from obligate clonal reproduction of C. japonica. Significant differences in allele frequency were detected among populations at eight and nine of nine polymorphic loci for C. soldanella and C. japonica (P<0.01), respecitvely. Considering the ecological importance of C. soldanella, the isolated beach populations coupled with present destruction of natural habitats of the species may result in erosion of genetic diversity in the near future. In this respect, conservation efforts should be focused on those populations that currently maintain the most genetic diversity such as those populations in the eastern and southeastern Korean Peninsula and Hamduck Beach, Cheju Island.

• 한국잡초학회지
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• 제4권2호
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• pp.149-153
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• 1984

전작(田作) 및 과수원(果樹園)의 주요(主要) 다년생(多年生) 잡초(雜草)의 하나인 메꽃(Calystegia japonica Choisy)의 생육(生育)에 미치는 pH, 건조시간(乾燥時間), 발생심도(發生深度), 토양수분(土壤水分) 및 광도(光度)의 영향(影響)을 조사(調査)하였다. 메꽃 지하경(地下莖) 생존율(生存率)은 $35^{\circ}C$에서 14시간(時間) 이상(以上) 건조(乾燥)되면 급격(急激)하게 감소(減少)되고, 생존(生存)되더라도 그 생육(生育)은 극히 저조(低調)하였으며, 발생심도(發生深度)가 0cm 혹은 8cm 이하(以下)로 될 때에는 생육(生育)이 크게 떨어졌다. 토양수분(土壤水分) 40~60%가 생육(生育)에 최적수준(最適水準)이었다. 메꽃의 초장(草長)은 광도(光度)의 증가(增加)로 감소(減少)되었지만, 근장(根長)과 건물중(建物重)은 증가(增加)하였다.

• 한국환경복원기술학회지
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• 제13권2호
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• pp.24-41
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• 2010

Distributional characteristics of vascular plants and plants selection for revegetation in bare ground of lakeside was investigated in nine artificial lakes. The vascular plants were composed of 64 families, 194 genera, 268 species, 3 subspecies, 30 varieties, 3 forma, totaling 304 taxa. Dominant species of nine investigated areas were represented as Ambrosia trifida, Calystegia hederacea, Matricaria matricariodes, Xanthium italicum. Dominant species of three parts based on the location of artificial lakes were as follows:Ambrosia trifida, Bidens tripartita, Calystegia hederacea, Equisetum arvense, Setaria viridis, Spergula arvensis, Torilis japonica in northern part (Lake Paro and Soyang); Calystegia hederacea, Matricaria matricariodes, Rumex crispus, Xanthium italicum in middle part (Lake Chungju, Daecheong, Andong and Imha); and Ambrosia artemisiifolia, Bidens frondosa, Chenopodium glaucum, Miscanthus sinensis var. purpurascens, Persicaria pubescens, Setaria viridis in southern part(Lake Juam, Hapcheon and Jinyang). Among the 304 taxa, Korean endemic, endangered and rare plants were not investigated. The naturalized plants were 46 taxa, and life form was Th-$R_5-D_4$-e type. Above the results, Calystegia hederacea, Equisetum arvense, Xanthium italicum, Matricaria matricariodes, and Spergula arvensis were considered with proper species for revegetation in bare ground of lakeside in artificial lakes.

• 한국약용작물학회지
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• 제14권6호
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• pp.347-353
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• 2006

This study was conducted to evaluate the vegetation structure and ordination of the Peucedanum japonicum Thunb. community by the Phytosociological method, floristic composition table on east coast of South Korea. The vegetation of Peucedanum japonicum Thunb. community was classified into 2 communities such as the Aster Spathulifolius community and the calystegia soldanella-Artemisia princeps community. Vegetation of the Peucedanum japonicum Thunb. community with the Aster spathulifolius community was shown southward, whereas vegetation of Peucedanum japonicum Thunb. community with the calystegia soldanella-Artemisia princeps community was shown northward from the base line of Pohang at North latitude of 36" 05'. Accordingly, the Peucedanum japonicum Thunb. community was grown commonly with the Aster spathulifolius community southward of the base line of Pohang, whereas it was grown commonly with the calystegia soldanella-Artemisia princeps community northward on the east coast. All environmental conditions of habitat taken together, the optimum habitat of Peucedanum japonicum Thunb. was at Ulsan geographically and the middle region of sea cliff topography, and was suitable for alkali. sandyloam.

• 대한의생명과학회지
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• 제15권4호
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• pp.335-342
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• 2009

This study was investigated to evaluate the contents of polyphenols and flavonoids, and physiological activities of various extracts from Calystegia japonica roots for making good use of their functional materials. The roots of C. japonica were extracted with water (WE), ethanol (EE) and hot water (HWE) by different methods. Among these extracts, the highest extracting yield was 30.30% of HWE, while the highest contents of total polyphenols and flavonoids were 40.85% and 6.40% of WE, respectively. The nitrite scavenging abilities were ranged from 31.31% (HWE) to 37.46% (EE) at pH 1.2 and 1.0 mg/ml concentration. In the measurements of electron donating abilities, EE showed the highest effect as 91.83% at 0.3 mg/ml assay concentration, and the electron donating ability was decreased as the extract concentration was increased. In the results of superoxide dismutase (SOD)-like activity, HWE showed the highest effect as 7.15% at 1.0 mg/ml. The tyrosinase inhibition activities of WE and EE were 15.28% and 14.97%, respectively. The xanthine oxidase inhibitory effects were ranged from 97.50 to 99.28% at 1.0 mg/ml. These results indicate that C. japonica extract has a good antioxidant effects and could be useful for developing functional products.

• Journal of Ecology and Environment
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• 제30권2호
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• pp.135-142
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• 2007

We used the Braun-Blanquet method to study coastal dune vegetation of South Korea. Coastal vegetation was monitored in thirty sites from April 2004 to September 2005. Seventeen plant communities were classified into five groups as follows: A. Two associations of herbaceous sand dune plants in Salsoletea komarovii Ohba, Miyawaki et Tx. 1973 (Calystegia soldanella community, Lathyrus japonicus-Calystegia soldanella community), B. Twelve associations of herbaceous perennial sand dune plants in Glehnietea littoralis Ohba, Miyawaki et Tx. 1973 (Carex pumila community, Elymus mollis community, Vitex rotundifolia-Elymus mollis community, Ixeris repens community, Elymus mollis-Ixeris repens community, Lathyrus japonicus community, Messershmidia sibirica community, Glehnia littoralis community, Carex kobomugi community, Calystegia solda-nella-Carex kobomugi community, Ishaemum anthephoroides community, Zoysia macrostachya community), C. One association of shrubby perennial sand dune plant in Viticetea rotundifoliae Ohba, Miyawaki et Tx. 1973 (Vitex rotundifolia community), D. One association of shrubby perennial sand dune plant in Rosetea multiflorae Ohba, Miyawaki et Tx. 1973 (Rosa rugosa community), E. The naturalized community (Diodia teres community).