• Korean Journal of Veterinary Research
• /
• v.47 no.2
• /
• pp.139-145
• /
• 2007

Measurement uncertainty could play an important role in the assessment of test results in laboratories and industries. We investigated measurement uncertainties possibly included in determination of flubendazole, a benzimidazole anthelmintic, in pork by HPLC. The concentration of flubendazole was 62.69 ng/g in a sample of pork. Uncertainty was estimated in the analytical procedure of flubendazole. A model equation was made for determination of flubendazole in pork. The four uncertainty components such as weight of sample, volume of sample, calibration curve, and recovery were selected to estimate measurement uncertainties. Standard uncertainty was calculated for each component and all the standard uncertainties were combined. The combined standard uncertainty was expanded to a sample population as an expanded uncertainty. The expanded uncertainty was calculated using k value on Student's t-table and effective degrees of freedom from Welch-Satterthwaite formula. The expanded uncertainty was calculated as 3.45 with the combined standard uncertainty, 1.584 6 and the k value, 2.18. The final expression can be ($62.69{\pm}3.45$) ng/g (confidence level 95%, k = 2.18). The uncertainty value might be estimated differently depending on the selection of the uncertainty components. It is difficult to estimate all the uncertainty factors. Therefore, it is better to take several big effecting components instead of many small effecting components.

• Journal of the Korean Society for Nondestructive Testing
• /
• v.33 no.1
• /
• pp.14-19
• /
• 2013

Nonlinearity parameter is an inherent property of materials measuring fundamental acoustic amplitude($A_1$) and second harmonic amplitude($A_2$). However, measurement of $A_1$ and $A_2$ has complex calibration procedure, many researchers prefer to measure relative nonlinearity parameter rather than absolute nonlinearity parameter. But, relative nonlinearity parameter is only detect materials degradation with various degradation samples, it is limited application in determining third order elastic constants of materials. Therefore, in this study, the piezoelectric detection method is adopted to measure absolute nonlinearity parameter due to experimental simplicity compare to capacitive detector. Linearity of measurement system is verified by $A_1^2vsA_2$ plot, and we measured ultrasonic nonlinearity parameters of fused silica and Al2024-T4.

• Journal of The Korean Society of Inherited Metabolic disease
• /
• v.15 no.3
• /
• pp.138-146
• /
• 2015

Purpose: If early diagnosis is not made, patients with metabolic disorders as homocystinemia rapidly progress to physical defect or mental retardation resulted in storage of the toxic material into the brain. Therefore, it is necessary to develop an analytical method for a rapid screening and/or correct confirmation diagnosis. Methods: The standard solution of sulfur amino acids spiked plasma was subjected to protein precipitation with methanol, and then consecutively derivatized with trimethylsilyl (TMS) and trifluoroacyl (TFA) and determined by GC-MS. The formation of TMS derivative of the hydroxyl and TFA derivative of amino functional group was performed by BSTFA and MBTFA, respectively. Selective ion monitoring (SIM) mode was used for quantification with selected specific ions. Results: A calibration curve on standard spiked pooled plasma showed a linear relationship with correlation coefficient of 0.9936-0.9992 for all compounds over the range of 0.1-300 ng. The precision and accuracy were within S.D. of 1-15% and RSD of 1-15% for intra-day assay at 2 ng/mL, 15 ng/mL and 30 ng/mL. LOD and LOQ was 0.4 ng/mL and 4 ng/mL respectively. Conclusion: A rapid analytical method was developed to quantify sulfur amino acids and methyl malonic acid, after two-step derivatization procedure with good sensitivity and specificity on human plasma. Advantages of a new method are simplicity and rapidity. The method could be useful for routine analysis, diagnosis of homocysteinemia.

• Journal of the Korean Society of Groundwater Environment
• /
• v.5 no.4
• /
• pp.210-214
• /
• 1998

In general the concentration of uranium in natural water such as fresh water and sea water is in the range of 0.01∼5 ppb, therefore trace analytical technique is required. The aim of present work is to compare a direct and preconcentration methods by evaporation and to investigate rapid and accurate trace analysis of uranium in groundwater using Instrumental Neutron Activation Analysis (INAA) which are sensitive and nondestructive method. Identification of analytical procedure was carried out using uranium standard solution of the range of 0.5∼100 ppb. In the given concentration, the deviation of calibration curve was less than 2%, and the standard deviation of measured values at each concentration was the range of 2∼12%. The difference of U content with sampling time for the same sample site was about 10.3%. Using this established method, the concentrations of uranium in samples collected at the 17 spring of Choongchung areas were found to be in the range of 1∼80 ppb.

• Analytical Science and Technology
• /
• v.28 no.1
• /
• pp.1-7
• /
• 2015

4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), a tobacco specific nitrosamine found only in tobacco products. The ability to monitor biomarker concentrations is very important in understanding environmental tobacco smoke (ETS). In this study, an efficient and sensitive method for the analysis of NNK in dust was developed and validated using liquid chromatography tandem mass spectrometry. Dust was collected with filter paper soaked in methanol. The standard solution and dust sample were diluted with 100 mM ammonium acetate and extracted using dichloromethane. Our calibration curves ranged from 25 to $10^4pg/mL$. Excellent linearity was obtained with correlation coefficient values between 0.9996 and 1.0000. The limit of detection (LOD) was 5 pg/mL ($S/N{\geq}3$) and the retention time was 10 min. The limit of quantification (LOQ) was 25 pg/mL, and the acceptance criteria was the rate of 98-103% (80-120% at levels up to $3{\times}LOQ$). The coefficient of variations (CV) was 2.8%. Accuracies determined from dust samples spiked with four different levels of NNK racurves ranged that from 25 to 104 pg/mL. Excellent linearity was obtained between 92.1% and 114%. The precision of the method was acceptable (5% of CV). The recovery rates of the whole analytical procedure at low, medium, and high levels were 105.7-116.5% for NNK. The carry-over effects during LC-MS/MS analysis were not observed for NNK. This manuscript summarizes the scientific evidence on the use of markers to measure ETS.

• Journal of Sensor Science and Technology
• /
• v.12 no.2
• /
• pp.79-87
• /
• 2003

In this paper, we propose a method to obtain a linear characteristic using soft computing for systems which have array sensors of nonlinear characteristics. Also a procedure utilizing the pattern information of array sensors without additional sensors is proposed to reduce disturbance effects. For a typical example, even a single CdS cell for CdS array has nonlinear characteristics. Overall linear characteristic for CdS array is obtained using fuzzy logic for each cell and overlapped portion. In addition, further improvement for linearization is obtained applying genetic algorithms for the parameters of membership functions. Also the effect of disturbing external light changes to the CdS array can be reduced without using any additional sensors for calibration. The proposed method based on fuzzy logic shows improvements for position measurements and disturbance reduction to external light changes due to the fuzziness of the shadow boundary as well as the inherent nonlinearity of the CdS array. This improvement is shown by applying the proposed method to the ball position measurements of a magnetic levitation system.

• Proceedings of the Korean Society for Food Science of Animal Resources Conference
• /
• 2000.11a
• /
• pp.1-14
• /
• 2000

The Near Infrared region of the energy spectrum was first discovered by Hershel in the year 1800. The principles of NIR is based on light absorption of specific organic chemical bonds. The absorption at each wavelength is measured and a spectre is obtained. The spectre is then treated mathematically and with the absorption data is converted to absolute units via a calibration. In the last two decades it has developed dramatically. With the invention of computers and the ability to treat a large amount of data in a very short time the use of NIR for many different purposes has developed very fast. During the last decade with the aid of very powerful PC's the application of NIR technology has become even more widespread. Now or days development of very robust calibrations can be done in a relatively short time with a minimum of resources. The use of Near Infrared Spectroscopy (NIR) in the Meat industry is relatively new. The first installations were taken into operation in the 80ties. The Meat Industry in often referred to as rather conservative and slow to embrace new technologies, they stay with the old and proven methods. The first NIR instruments used by the Meat Industry, and most other industries, were multipurpose build, which means that the sample presentation was not well suited to this particular application, or many other applications for that sake. As the Meat Industry grows and develops to meet the demands of the modern markets, they realise the need for better control of processes and final products. From the early 90 ties and onward the demand for 'rear time' rapid results starts growing, and some suppliers of NIR instruments (and instruments based on other technologies, like X-ray) start to develop and manufacture instrumentation dedicated to the particular needs of the Meat Industry. Today it is estimated that there are approximately 2000 rapid instruments placed in the Meat industry world-wide. By far most of these are used as at-line or laboratory installations, but the trend and need is moving towards real on-line or in-line solutions. NIR is the most cost effective and reproducible analytical procedure available for the twenty first century.

• The Journal of Advanced Prosthodontics
• /
• v.11 no.6
• /
• pp.350-357
• /
• 2019

PURPOSE. This study aims to evaluate the loosening torque on the implant fixture, and to assess the accuracy of difference electronic torque drivers. MATERIALS AND METHODS. Three electronic torque drivers were used to measure the loosening torque on the implant system (AnyOne; MegaGen). The implant fixtures were divided among the 3 electronic torque driver types (W&H, SAESHIN, and NSK group) and 9 for each group. The screws were fastened at the implant fixture by three electronic torque drivers using the tightening torques recommended by the manufacturers of the drivers. After 10 minutes, the screws were again fastened at the implant fixture with equal torque. Then, the loosening torques were measured with an MGT12 torque gauge (MARK-10, Inc.). This measurement procedure was repeated 10 times under loosening torques of 15 Ncm, 25 Ncm, and 35 Ncm. In the statistical analysis, all values of loosening torque were analyzed with the one-way ANOVA and Kruskal-Wallis test (α=.05) for comparative evaluation. RESULTS. There were significant inter-group differences at loosening torques of 15 Ncm and 25 Ncm (P<.05). The accuracy of the NSK driver was the highest, followed by SAESHIN and W&H. There was no significant difference between NSK and W&H at 35 Ncm (P>.05). The SAESHIN driver showed the closest loosening torque at 35 Ncm. CONCLUSION. The most accurate loosening torques were SAESHIN at 35 Ncm, and NSK at 15 Ncm and 25 Ncm. Since the loosening torque may vary depending on the tightening torques and electronic torque drivers, periodic calibration of the electronic torque driver is recommended.

• Journal of Korean Society for Geospatial Information Science
• /
• v.10 no.3 s.21
• /
• pp.17-27
• /
• 2002

It is desirable to use a photogrammetric scanner to minimize the errors from the scanning procedure in case of building aerial photo image DB. The photogrammetric scanner, however, is highly expensive to have only limited number of scanners available, therefore the general-purpose scanners have been considered as an alternative. This study has mainly been focused to see the possibility of the general-purpose scanner to be used for scanning aerial photographs. For that, the analysis of the image coordinates were made as well as the accuracy evaluation of each phase. In addition to that, as the application of the orthophoto images has been increased, the magnitudes of the errors from the DEM generation and orthophoto projection were analyzed. Also, the calibration program was developed to verify the geometric distortion of the automatic scanner to contribute to enhance the quality of the aerial photo image DB.

• Bulletin of the Korean Chemical Society
• /
• v.25 no.8
• /
• pp.1177-1181
• /
• 2004

The determination of uric acid in urine samples was studied by a chemiluminescence measurement using tris (2,2'-bipyridine)ruthenium(II)-octylphenylpolyglycolether [Ru$(bpy)_3^{2+}$ -OPE] system. The oxidized uric acid by Ce(IV) excited Ru$(bpy)_3^{2+}$ to emit a chemiluminescence in this system so that the intensity was stoichio-metrically dependent upon the concentration of uric acid. In a reaction cell, a luminescent reagent, oxidant, surfactant and sulfuric acid were flowed into and mixed with a taken sample. Experimental conditions were optimized to obtain the maximum intensity of chemiluminescence. Each reactant solution of more than the following concentration gave a good result: $2\;{\times}\;10^{?4}$ M Ru$(bpy)_3^{2+}$ , 0.01 M Ce(IV), 6% OPE, and 0.33 M $H_2SO_4$. Any interferences were not shown in this process by the investigation of concomitant constitutes such as albumin, creatine, lactic acid, glucose, urea, $Cl^?,\; Mg^{2+},\;Ca^{2+}$ and so on. The linearity of a calibration curve was good with r = 0.998, the relative standard deviation of the slope was 3.3% and the detection limit was 5.6ng/mL. The recoveries of 80 to 91% were obtained from the standard spiked samples. The values were little bit low, but this procedure could be considered to be reliable for the determination of trace uric acid in urine samples.