• Journal of Animal Science and Technology
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• v.51 no.4
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• pp.295-306
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• 2009

This experiment was conducted to investigate the effects of dietary calcium (Ca) and vitamin $D_3$ metabolite on eggshell quality and hatching egg production in aged egg-type breeder hens. A total of five hundred and forty 60-week-old Hy-Line Brown breeder hens were randomly allocated to nine treatments in a $3\times3$ factorial design to determine the effects of dietary Ca levels (3.3, 3.9, and 4.5%), combined with three levels of 25-hydroxycholecalciferol [25-$(OH)D_3$ 0, 75, and $150{\mu}g/kg$], on eggshell quality and egg production for 10 weeks. There were significant Ca and 25-$(OH)D_3$ effects (P<0.01) on egg production rate. There was a linear (P<0.01) increase in eggshell strength and thickness with increasing dietary Ca and 25-$(OH)D_3$ levels. Dietary 25-$(OH)D_3$ had a significant effect (P<0.05) on hatchability of egg although fertility was not influenced by dietarylevels of Ca and 25-$(OH)D_3$. Dietary Ca levels affected Ca content in tibia (P<0.05). These results indicated that relatively high levels of dietary Ca in combination with 25-$(OH)D_3$ had a beneficial effect on improving eggshell quality and reproductive performance in aged egg-type breeder hens.

• Clinical and Experimental Reproductive Medicine
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• v.35 no.3
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• pp.203-212
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• 2008

Objective: During cryopreservation process, cold shock and cryo-injury affect the fertilizing capacity of the sperm by damaging cell membranes with loss of functional integrity. A longstanding concept for preventing the cryo-damage is to stabilize the plasma membrane by incorporating cholesterol. This study was to determine the effects of cholesterol in freezing media on the motility and functional integrity of human sperm after cryopreservation. Methods: Control group (non-cholesterol treated) and different concentrations of cholesterol-treated sperm (14 healthy males) were frozen and thawed. After freezing and thawing of sperm, the quality of sperm was evaluated by sperm analysis, acrosome reaction test and sperm chromatin structure assay. Results: When human sperm were incubated in sperm freezing medium (SFM) containing $0.5{\mu}g$ cholesterol and then freezing/thawing, the motility of sperm have significantly improved compared to those untreated cholesterol ($33.46{\pm}1.48%$ vs. $30.10{\pm}1.07%$, p<0.05). The rate of calcium ionophore-induced acrosome reactions in post-thawed sperm was significantly higher than that ($53.60{\pm}1.60%$ vs. $47.40{\pm}1.86%$, p<0.05) in SFM containing cholesterol. Sperm chromatin structure assay revealed that DNA damage to the sperm in the cholesterol-treated group was lower than that of non-treated group. Conclusion: These results suggest that increased cholesterol content of sperm plasma membrane by supplementation of cholesterol in SFM improves sperm motility, capacitation status, and DNA integrity. Therefore, addition of cholesterol into SFM could be a useful for protecting human sperm from cold shock and cryo-injury during cryopreservation.

• Food Science and Preservation
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• v.15 no.1
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• pp.161-168
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• 2008

The effects tea beverages (TBs) prepared from powdered green tea (Gt), oolong tea (Ot), black tea (Bt), or pure tea (Pt) with lemon, orange, grenadine etc on cadmium toxicity in rats were investigated Sensory evaluations of the TBs are better than those of each water extracted teas. Cadmium (50 ppm) was administered to experimental rats fed a basic diet, or a diet with various TBs (15% w/v), for 5 weeks. Although body weight gains, feed intakes, and fecal weights in all Cd-treated groups were lower than those in the normal control group (NC), feed efficiency ratio, urine volumes, liver weights, and kidney weights did not differ significantly between groups. The serum ALT and AST levels in the Cd-treated control group (Cd-Co) were higher than those in the NC animals. Serum ALT and AST levels in all Cd-treated rats fed TB-supplemented diets were lower than in animals of the Cd-Co group. Tibia and femur weights in Cd-Co animals were lower than those in NC rats. Tibia and femur weights in Cd-treated rats fed TB-supplemented diets were higher than those in Cd-Co animals. There were no between-group differences in tibia lengths; animals in the NC and TB-supplemented diet groups showed femur lengths longer than those of Cd-Co rats. Although the contents of crude ash and cadmium in the femurs of Cd-Co mts was markedly higher than in the femurs of NC animals, the cadmium content in femurs of Cd-Co rats was significantly lower than that in the femurs of NC animals. The changes in mineral levels caused by Cd administration were alleviated by every TB-supplemented diet tested Whereas fecal calcium excretion by Cd-Co animals was significantly higher than that of NC rats, calcium excretion by Cd-treated rats fed TB-supplemented diets was significantly lower than that of Cd-Co animals. Fecal cadmium excretion by all Cd-treated rats fed TB-supplemented diets was significantly higher than that of Cd-Co animals. In conclusion, this study provides experimental evidence that various TBs may regulate cadmium-induced organ toxicity by reducing cadmium accumulation in tissues through the mechanism of increasing the fecal excretion of cadmium.

• Applied Biological Chemistry
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• v.11
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• pp.173-184
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• 1969

To elucidate the effect of Amo-1618(4-hydroxyl-5-isopropyl-2-methlphenyl trimethyl ammonium chloride, 1-piperidine carboxylate) known as a kind of growth retardant, on the growth, grain yield, increasing the efficiency of nitrogen fertilizer, behavior of mineral nutritions and the rate of phosphorus utilization, this experiment was conducted pot culture method in a vinyl house. Two nitrogen level, namely, practical nitrogen level(1 N) and three times nitrogen level(3 N) was made and labelled double-calcium-superphosphate $Ca(H_2P^{32}O_4)_2.\;2H_2O)$ as a source of radioactive phosphorus(P-32) was employed $80\;{\mu}c/pot$, respectively. Rice seedlings, variety 'Suwon No. 82', was transplanted to a 1/50,000 a china pot on June 13 in 1968. For treatment, at early stage of tillering, 10,000 ppm solution of Amo-1618 was foliar sprayed only one time. The Duncan's new mutiple-range test was adopted for statistical analysis evaluating experimental data at 5% level significance. The results obtained may be summarized as follows; 1) No significant difference was found among the treatments in plant height, but in plot of Amo-1618 treatment and 3 N level, number of tillers was significantly increased than that of others. 2) Weight of 10,000 kernels and seed-setting rate was also remarkably increased in same treatment above. 3) Grain yield per pot was significantly increased in Amo-1618 and 3 N level application. This results seemed to be due to the increased the factors on the yield. 4) Contents of nitrogen and phosphorus per cent in the grain was likewise increased in Amo-1618 and 3 N application. There is, however, no difference among treatments in the content of nitrogen and phosphorus in the leaves and culms of rice plants. 5) On the other hand, the contents of potassium and magnesium, no distinctly tendeny showed among treatments. 6) The rate of phosphorus utilization was significantly increased in the plot of Amo-1618 and 3 N application.

• Journal of Chest Surgery
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• v.42 no.6
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• pp.685-695
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• 2009

Background: Calcification is the most frequent cause of clinical failure of bioprosthetic tissues that are fabricated from Glutaraldehyde (GA)-fixed porcine valve or bovine pericardium. We recently used a multi-factorial approach of employing different mechanisms to investigate how to reduce the calcification of bioprosthetic tissues. The purpose of the present study was to evaluate the synchronized synergism using ethanol, L-lysine and $NaBH_4$ in glutaraldehyde treated porcine pericardium from the standpoint of calcification and tissue elasticity. Material and Method: Porcine pericardium was fixed with 0.625% GA (commercial fixation). An interim step of ethanol (80%; 1 day at room temperature) or L-lysine (0.1 M; 2 days at $37^{\circ}C$) or $NaBH_4$ (0.1 M; 2 days at room temperature) was followed by completion of the GA fixation (2 days at $4^{\circ}C$ and 7 days at room temperature). The tensile strength and thickness of the samples were measured. The treated pericardiums were implanted subcutaneously into three-week old Sprague-Dawley rats for 8 weeks. The calcium content was assessed by atomic absorption spectroscopy and the histology of the samples. Result: The amount of calcium in the pericardium pretreated with ethanol (13.6${\pm}$10.0 ug/mg, p=0.008), L-lysine (15.3${\pm}$1.0 ug/mg, p=0.002) and both (16.1${\pm}$11.1 ug/mg, p=0.012) was significantly reduced compared with the control (51.2${\pm}$8.5 ug/mg). However, $NaBH_4$ pretreatment (65.7${\pm}$61.8 ug/mg, p=0.653) and combined pretreatment that including ethanol, L-lysine and $NaBH_4$ (92.9${\pm}$58.3 ug/mg, p=0.288) were not significantly different from the controls(51.2${\pm}$8.5 ug/mg). Both the combined pretreatment using ethanol and L-lysine (7.60${\pm}$1.55, p=0.76) and the combined pretreatment that included ethanol, L-lysine and $NaBH_4$ (7.47${\pm}$1.85, p=0.33) increased the tensile strength/thickness ratio compared with that of the controls (4.75${\pm}$1.88). Conclusion: The combined pretreatment using ethanol and L-lysine seemed to decrease the calcification of porcine pericardium fixed with glutaraldehyde, as compared to single pretreatment, and it increase the tissue elasticity, but to the degree that showed synchronized synergism. $NaBH_4$ pretreatment seemed to increase the calcification of porcine pericardium, irrespective of whether single or combined pretreatment was used.

• Korean Journal of Soil Science and Fertilizer
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• v.16 no.3
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• pp.235-241
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• 1983

Major cation uptake by the rice plant and its leachates in submerged condition were studied at 3 different levels of potassium and nitrogen application with three texture soils (Clay loam, Loam, Sandy loam) by pot experiment. The results are as follows. 1. Potassium uptake and grain yields of rice plant were increased and calcium and magnesium uptake of rice plant were decreased by application of potassium. 2. The potassium application caused to increase Ca, Mg, K and $NH_4$ Content in leachate. 3. In the rice leaf at heading stage, the optimum cation ratios of K/Ca, K/Mg in me and $K_2O/N$ in % at N 3.3g/pot level were 1.59, 4.26 and 3.62, respectively, but the ratios were increased to 1.65, 4.32 and 3.94 at high level of nitrogen. 4. Similar trends of cation ratios were found in rice straw. leaching soil solution and soils after harvest by potassium application.

• Korean Journal of Soil Science and Fertilizer
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• v.17 no.3
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• pp.253-257
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• 1984

A field experiment was conducted in order to secure the basic information on the rational application of nitrogen and potassium for autumn growing Chinese cabbage (Brassica campastris ssp. pekinensis, var.; Miho 70 days). The results of the experiment are summarized as follows: 1. Potassium effect was observed where nitrogen applied either less than 15kg N/10a and heavy dose of 25kg N/10a. No potassium effect was observed where 20kg N/10a applied. In the case of 25kg N/10a, potassium effect was observed only in total weight but failed yield marketable product weighing over 1 kilogram per cabbage. 2. Nitrogen application, in general, tends to lower the soil pH and it is particularly true when heavy dose of over 20kg N/10a is applied. As a result, Chinese cabbage has increasingly removed soil born potassium and reduced exchangeable potassium content of the soil. 3. Oven dried cabbage which received 25kg N/10a plus potassium showed a low concentration of calcium and this phenomena seems to be attributable to the acidification of soils and it further caused failure in producing cabbages of marketing value.

• The Korean Journal of Food And Nutrition
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• v.24 no.4
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• pp.520-527
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• 2011

This study was conducted to develop a novel salad dressing composite recipe of natural seasoning containing the dried sea tangle($Laminaria$ $japonica$) that has a high preference. Sea tangle($Laminaria$ $japonica$) is included in the vitamins and minerals, magnesium, calcium, iodine, iron content, such as high, and contained in Sea tangle alginate is not a small conference known as dietary fiber. To manufacture salad dressing with sea tangle, dressing with 0%, 3%, 6%, 9%, and 12% added sea tangle were prepared and tested for quality. The pH tended to increase with the increased sea tangle in addition but in contrast, acidity showed. The 'L' color decreased with added sea tangle, whereas the 'a' and 'b' values increased. Brix measurements increase with added sea tangle. The strength texture results, 0% was the highest, lowest 9%. Bitterness and chewiness texture results, 9% was the highest, lowest 0%. According to the sensory test results, in the topic overall quality 3% was by 3.76 point the highest. But, during total nine clause, in clause six, by 6% was the highest.

• Korean Journal of Organic Agriculture
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• v.21 no.4
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• pp.753-767
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• 2013

Soil contamination has continuously increased worldwide, thus the remediation for the contaminated soils has risen steadily. However, the consideration of ecological safety for the remediated soils and their agricultural uses has been very limited. Therefore, this study was to investigate the influences of selected environmentally-friendly agricultural materials, organic by-product fertilizer (OF), charcoal (CC), and biochar (BC), as soil conditioners for improving poor chemical properties of fuel-oil removed soil by land farming technique. Two different remediated soils, remediated soil A (RSA) and remediated soil B (RSB), were selected. Soil texture of both RSA and RSB was sandy loam. The chemical properties of RSA and RSB were as follows: soil pHs of 8.5 and 8.7, soil organic matter contents of 7.4 and 5.5g $kg^{-1}$, total nitrogen contents of 0.26 and 0.10g $kg^{-1}$, available phosphorus concentrations of 7.2 and 4.4mg $kg^{-1}$, and exchangeable calcium concentrations of 14.8 and $11.7cmol_c$ $kg^{-1}$, respectively. Results of the properties were not reached for the optimal values for cultivating crops that were recommended by National Academy of Agricultural Science at Rural Development Administration in Korea. However, after applying OF, CC, and BC, the chemical properties of soils were selectively improved, which were that soil organic matter content and available phosphorus concentration increased, whereas the soil pH were not changed. In particular, the chemical properties were positively changed more with the application of 5.0% biochar. Thus, continuous management of the remediated soils with applying the eco-friendly agricultural materials can improve the quality of reme-diated soils.

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.12
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• pp.1761-1768
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• 2005

Effects of alpha-galactosidase (GAL) on broiler corn-soybean meal diet was investigated. In experiment 1, sixty cockerels were allocated to five groups, including three enzyme treatments (GAL added at 0, 500, and 1,000 mg/kg diet), a nitrogen-free diet group and a fast group. The true nitrogen-corrected ME (TME$_n$) and true amino acid availability were determined. In experiment 2, 324 day-old chicks were used in a 2${\times}$3 factorial design consisting of two energy contents (high and low) and three GAL levels (0, 250, and 500 mg/kg). Three feeding phases, comprising 0-21 d, 22-35 d and 36-48 d, were involved. GAL addition improved TME$_n$ and the availability of methionine and cystine (p<0.05). The apparent ME (AME) or nitrogen-corrected AME (AME$_n$) and digestibility of dry matter, organic matter, calcium, and phosphorus were improved significantly on d 21, so was crude protein and an interaction of energy and GAL on AME$_n$ (p<0.05) was found on d 35. However, daily intake and daily gain were significantly improved with GAL addition (p<0.05) during 21 d. The small intestine relative weight decreased at 250 mg/kg GAL (p<0.05) on d 35, whereas presented an interaction between GAL and energy on d 21 (p<0.05). Likewise, this treatment increased breast muscle ratio (p<0.05). On d 21, triglycerides level of broilers showed interaction between energy and enzyme levels (p<0.05). Uric acid level in 500 mg/kg GAL declined linearly (p<0.05). On d 35, quadratic effects (p<0.05) were observed in total protein, albumin, globulin and cholesterol content for enzyme supplementation. And the interactive effects of energy and GAL on serum values showed more obviously. The study implies that GAL improved energy and nutrient availability of corn-soybean meal diet in broiler. The GAL supplementation to corn-soybean meal based diet can improve performance of broilers in early stages of growth.