• Title/Summary/Keyword: Calanthe discolor Lindl.

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Aseptic Germination of F1 Hybrid Seed by Inter-species Pollination of Calanthe discolor Lindl. and C. discolor for. Sieboldii (Decne.) Ohwi (새우난초(Calanthe discolor Lindl.)와 금새우난초[C. discolor for. sieboldii (Decne.) Ohwi]의 종간교배에 의한 1대잡종 종자의 기내무균 발아)

  • Kim, Kwang-Soo;Kim, Jong-Sun;Park, Jong-Hwan
    • Korean Journal of Plant Resources
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    • v.21 no.4
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    • pp.341-345
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    • 2008
  • Two orchid species of Calanthe discolor Lindl. and C. discolor for. sieboldii (Decne.) Ohwi, which have different form flower color and size. They were crossed in mid April by artificial pollination, and the F1 hybrid seeds were collected mid October. Germination of seeds was investigated on pre-treatment of seeds and under the various environmental conditions. Germination was promoted by moisture absorption and ultrasonic treatment of seeds. Dark culture of F1 hybrid seeds enhanced germination and protocorm formation, and development into seedlings compared with light culture. Although, plant growth regulators such as NAA and BA had a slightly promotive effect on seed germination and protocorm growth, regenerated seeding were showed abnormal growth patterns. Regenerated F1 hybrid plantlets were successfully transferred to pot.

Asymbiotic seed germination and in vitro propagation condition in Calanthe discolor Lindl.

  • Kwon, Huyk Joon;Shin, So Lim;Bae, Kee Hwa;Kim, Soo-Young
    • Journal of Species Research
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    • v.6 no.1
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    • pp.68-75
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    • 2017
  • We investigated the effects of sodium hypochlorite (NaOCl) and culture medium on embryo swelling and germination of Calanthe discolor Lindl., and established a method for determining the swelling and protocorm formation of C. discolor seeds via in vitro examination of immature seeds. Treatment of immature seeds with NaOCl greatly enhanced the extent of embryo swelling and protocorm formation of immature zygote embryos compared to seeds without NaOCl treatment. The effects of the culture media were also evaluated with regard to embryo swelling and protocorm formation of in vitro cultured seeds with and without NaOCl treatment. Additionally, the effects of white fluorescent light and red and blue LEDs lights on seedling growth in in vitro culture were examined. The most suitable condition for seedling growth after 12 weeks of culture was the red LEDs light with POM medium. These results show effective asymbiotic germination and growth of C. discolor.

Micropropagation of Calanthe discolor Lindl. through Induction of Multiple Shoots from Axillary Bud Culture (새우난초(Calanthe discolor Lindl.)의 액아배양으로부터 다신초 형성을 통한 대량증식)

  • Lim, Ju Hong;Chung, Mi Young;Kim, Chang Kil;Lim, Ki Byung;Chung, Jae Dong
    • FLOWER RESEARCH JOURNAL
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    • v.16 no.4
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    • pp.239-246
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    • 2008
  • This study was conducted to establishment of in vitro micropropagation through induction of multiple shoots from axillary bud culture in Calanthe discolor Lindl. Shoots initiation from axillary bud was the most effective on half strength MS medium supplemented with $0.5mg{\cdot}L^{-1}$ NAA and $2.0mg{\cdot}L^{-1}$ TDZ during four weeks of dark culture followed by culture under a 16-h photoperiod. Multiple shoots (12.5 shoots per explant) were proliferated on half strength MS medium containing $4.0mg{\cdot}L^{-1}$ TDZ and $2.0mg{\cdot}L^{-1}$ IBA. On the other hands, the abnormally emerged shoots during the multiple shoot proliferation stage were recovered to normal shoots on half strength MS hormone free medium. Multiple shoots were well elongated and rooted on half strength MS medium with $0.1mg{\cdot}L^{-1}$ NAA. The plantlets were acclimatized up to 100% on TKS substrate after pretreating with $10mg{\cdot}L^{-1}$ NAA for 30 min. and these plantlets showed good growth as well.

Podospora leporina (Podosporaceae): An Unrecorded Endophytic Fungus Isolated from Cypripedium guttatum

  • Ju-Kyeong Eo;Juo Choi;Seung Won Lee;Nam Young Kim
    • The Korean Journal of Mycology
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    • v.50 no.4
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    • pp.361-365
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    • 2022
  • Here, Podospora leporina was isolated and cultured from Cypripedium guttatum Sw. (spotted lady's slipper), which was designated as an endangered wildlife class 1 by the Ministry of Environment. Further, Podospora sp., which is found in Calanthe discolor Lindl. (Japanese hardy orchid) root is recorded as an endophytic fungus in Jeju Island, Korea. However, there is no existing record of Podospora spp. Thus, in this study, we analyzed the morphological characteristics and two molecular markers, internal transcribed spacer (ITS) and large ribosomal subunit (LSU), of P. leporina and compared these data with other relative species. Consequently, this endophytic fungal species was confirmed as an unrecorded species in Korea, so we presented cultural characteristics and microstructures, such as the conidia of P. leporina.