• Title/Summary/Keyword: Cage

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Effect of Egg Yolks from Laying Hens Intubated Astaxanthin on the Oxidation of Liver Microsome of Mouse (Astaxanthin처리 산란계로부터 생산된 난황이 Mouse Liver Microsome의 산화에 미치는 영향)

  • 김홍출;박숙자;김정곤;박철우;조용운;조현종;하영래
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.31 no.1
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    • pp.155-159
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    • 2002
  • Effect of the egg yolky from laying hens intubated, p.o., astaxanthin (designated AEY) on the oxidation of mouse liver microtome was investigated using female ICR mouse (6~7 weeks of age). Mice were adapted in a temperature- and humidity-controlled house for one week and randomly divided into 7 treatment groups (10 mice/cage/treatment). Mice were intubated p.o., AEY (50, 100 and 150 mg) or control egg yolk (CEY, 150 mg) every week for 3 weeks. BHT (5 mg) and e -tocopherol (50 mg) were fed to mice as positive control. At week 4, the liver microsome was prepared from sacrificed mice. Protein content of mouse liver microsome with AEY treatment was relatively higher than that with CEY treatment. AEY treatment remarkably lowered the content of unsaturated fatty acids including oleic acid and linoleic acid, but raised that of the saturated fatty acids including stearic acid. AEY group showed relatively higher antioxidative activity than CEY, when used Asc/F $e^{+2}$ or NADPH/F $e^{+2}$ as oxidant. Antioxidative activity of AEY was more effective than $\alpha$-tocopherol, but less effective than BHT.

Sptimum Design of a Uniform Magnetic Field Exposure System for a Small-Sized Animal Study (자계 균일 공간 확보를 위한 소동물 실험용 5G급 자계 발생장치의 최적 설계)

  • 김상범;추장희;이동일;명성호
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.11 no.7
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    • pp.1194-1203
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    • 2000
  • A magnetic field exposure system that generates 60 Hz magnetic fields from 1 mG to 5 G was designed and constructed for small-sized animal study. In order to investigate as many animals as possible at one series of test, uniform magnetic fields are required at wide living area of the animals. In this article, a cubic shaped field exposure system with three animal living floors was designed, which offers about 50 seating capacity. For calculation of magnetic fields inside the cage, a three-dimensional calculation program was developed. Using this, optimum electric current ratio of inner coil to outer coil and position of each coil were determined. Meanwhile, inductance of the exposure system was calculated for the design of power supply. The field measurement results of the manufactured exposure system showed that the difference between maximum and minimum magnetic field at the testing floors was less than 3%, which strongly demonstrated the field exposure system was good for small sized animal study.

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Addition of a Worm Leachate as Source of Humic Substances in the Drinking Water of Broiler Chickens

  • Gomez-Rosales, S.;Angeles, M. De L.
    • Asian-Australasian Journal of Animal Sciences
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    • v.28 no.2
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    • pp.215-222
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    • 2015
  • The objective of this research was to evaluate the growth performance, the apparent ileal digestibility of nitrogen and energy, the retention of nutrients and the apparent metabolizable energy corrected to zero nitrogen retention (AMEn) in broiler chickens supplemented with increasing doses of a worm leachate (WL) as a source of humic substances (HS) in the drinking water. In Exp. 1, 140 male broilers were penned individually and assigned to four WL levels (0%, 10%, 20%, and 30%) mixed in the drinking water from 21 to 49 days of age. Water was offered in plastic bottles tied to the cage. In Exp. 2, 600 male broilers from 21 to 49 days of age housed in floor pens were assigned to three levels of WL (0%, 10%, and 20%) mixed in the drinking water. The WL was mixed with tap water in plastic containers connected by plastic tubing to bell drinkers. The results of both experiments were subjected to analysis of variance and polynomial contrasts. In Exp. 1, the daily water consumption was similar among treatments but the consumption of humic, fulvic, and total humic acids increased linearly (p<0.01) as the WL increased in the drinking water. The feed conversion (p<0.01) and the ileal digestibility of energy, the excretion of dry matter and energy, the retention of dry matter, ash and nitrogen and the AMEn showed quadratic responses (p<0.05) relative to the WL levels in drinking water. In Exp. 2, the increasing level of WL in the drinking water had quadratic effects on the final body weight, daily weight gain and feed conversion ratio (p<0.05). The addition of WL as a source of HS in the drinking water had beneficial effects on the growth performance, ileal digestibility of energy, the retention of nutrients as well on the AMEn in broiler chickens; the best results were observed when the WL was mixed at levels of 20% to 30% in the drinking water.

Effect of Particle Clogging in Orifices on the Particle Collection Efficiency of a Micro-Orifice Impactor (노즐 막힘이 미세 오리피스형 다단 임팩터의 입자 채취 성능에 미치는 영향)

  • Ji, Jun-Ho;Bae, Gwi-Nam;Hwang, Jung-Ho
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.27 no.2
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    • pp.197-205
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    • 2003
  • A cascade impactor is a multistage impaction device used to separate airborne particles into aerodynamic size classes. A micro-orifice impactor uses micro-orifice nozzles to extend the cut sizes of the lower stages to as small as 0.05 ${\mu}{\textrm}{m}$ in diameter without resorting to low pressures or creating excessive pressure drops across the impactor stages. In this work, the phenomenon of particle clogging in micro-orifice nozzles was experimentally investigated for a commercial micro-orifice uniform deposit impactor (MOUDI). It was observed, using an optical microscope, that the micro-orifice nozzles of the final stages were partially clogged due to particle deposition during the aerosol sampling. Therefore the pressure drops across the nozzles were higher than the nominal values given by the manufacturer. To examine the effect of particle clogging in micro-orifice nozzles, the particle collection efficiency of the MOUDI was evaluated using an electrical method for fine particles with diameters in the range of 0.1-0.6 ${\mu}{\textrm}{m}$. The monodisperse liquid dioctyl sebacate (DOS) particles were used as test aerosols. A faraday cage was employed to measure the low-level current of the charged particles upstream and downstream of each stage. It was found that the collection efficiency curves shifted to correspond to smaller orifice sizes, and the 50-% cutoff sizes were much smaller than those given by the manufacturer for the three stages with nozzles less than 400 ${\mu}{\textrm}{m}$ in diameter.

Significance of varying concentrations of T-2 toxin on growth performance, serum biochemical and hematological parameters in broiler chickens

  • Singh, Ram;Park, Sungkwon;Koo, Jin Su;Kim, In Ho;Balasubramanian, Balamuralikrishnan
    • Journal of Animal Science and Technology
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    • v.62 no.4
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    • pp.468-474
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    • 2020
  • The study was to determine the effects of diverse concentrations of T-2 toxin in broiler diet. Three hundred 1-day-old chicks with initial body weight of 46 ± 0.52 g were chosen and randomly assigned into five dietary treatments with 5 replicate cages and 12 broilers per cage for 42 d feeding trial. Dietary treatments were prepared with basal diets containing 0 (T1), 50 (T2), 100 (T3), 150 (T4), 200 (T5) ppb T2-toxin. Significant results were observed in the decreased intake of feed, feed conversion ratio (FCR), body weight gain (BWG), level of serum protein, cholesterol and hemoglobulin of broilers in increased concentration of the T-2 toxin in diet (150 and 200 ppb) groups than control. Also, observed that the uric acid, serum glutamic pyruvic transferase (SGPT), serum glutamic oxaloacetic transferase (SGOT) and Heterophil/Lymphocyte (H/L) ratio value were significantly higher (p < 0.05) in groups T4 and T5 than control. However, the BWG, feed intake and FCR, as well blood biochemical profiles of serum protein, cholesterol, hemoglobulin, uric acid, SGPT, SGOT and H/L ratio in groups T2 and T3 were statistically similar to control diet of broilers. It was concluded that the results showed that no adverse effects on growth performance and blood biochemical parameters in broilers feed with T-2 toxin (50 and 100 ppb) during the entire trial.

Effects of Baekgumhwan administration on immune-function in ICR mice stressed by electric footshock (백금환(白金丸)의 경구 투여가 전기자극 스트레스를 받은 mouse의 비장에 존재하는 면역 세포 분획과 사이토카인 생성에 미치는 영향)

  • Joo, Seung-Gyun;Kim, Geun-Woo;Koo, Byung-Soo;Shim, Sang-Min
    • Journal of Oriental Neuropsychiatry
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    • v.13 no.1
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    • pp.39-52
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    • 2002
  • The present experiments were designed to study the influence of Baekgumhwan on immune function of ICR mice under stress condition. Baekgumhwan was orally administered to the mice for 15days. on the 11th day the mice subjected to electric footshock for 5days(2 session a day, 11 footshocks a 31 min-session). B/T cell populations in splenocytes were studied by FACS analysis and cytokines($IFN-{\gamma}$ rand IL-10) production of the mouse splenocytes treated with PHA were studied by sandwich ELISA assay on the 15th day. The results were as follows. 1. After electric footshock, mice became sluggish and crowded to one side of the cage. Increased B/T cell populations in splenocytes were observed. These results confirm that electric footshock caused stress inducing immunological and behavioral changes in ICR mice. 2. Baekgumhwan administration without stress increase B cell populations in splenocytes, but T cell populations and cytokines($IFN-{\gamma}$ and IL-10) production of the mouse splenocytes treated with PHA maintain as similar levels as in the normal group. 3. Baekgumhwan administration with stress significantly antagonized the effect of electric footshock on behavior, increased B cell populations in splenocytes, so maintain as similar levels as in the normal group. cytokines($IFN-{\gamma}$ rand IL-10) production of the mouse splenocytes treated with PHA maintain as similar levels as in the normal group and T cell populations in splenocytes were increased as stress control.

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The Effect of Therapeutic Exercise on Brain-Derived Neurotrophic Factor After Global Brain Ischemia in Rats (흰쥐의 전뇌허혈 후 재관류 시 운동치료에 의한 신경영양성인자 발현)

  • Gu, Sang-Hun;Song, Ju-Young;Kown, Young-Shil;Nam, Ki-Won;Song, Ju-Min;Lee, Yun-Seob;Choi, Jin-Ho;Kim, Jin-Sang
    • The Journal of Korean Physical Therapy
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    • v.13 no.2
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    • pp.281-292
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    • 2001
  • This study was performed to investigate the effect of therapeutic exercise on brain-derived neurotrophic factor manifestation after global brain ischemia in rats. Nine rats with global ischemia were divided at random into two group. In the control group, three rats remained in cage. But, in the end, two rats were alive. In the therapeutic exercise group, six rats remained. The five rats of this group was swam for 30 minutes everyday for a week. The brain-derived neurotrophic factor expression was identified from immunohistochemistry. The results of this study were as follows : 1. In the control group, a little expression of brain-derived neurotrophic factor was observed at cortex and hippocampus layer, but cell body and axon was observed obscurely. 2. In the experimental group, a much expression of brain-derived neurotrophic factor was observed at cortex and hippocampus layer, and cell body and axon was observed clearly. In the neurological examination(beam-walking test). experimental group was obtained higher 1.4 points than control group. BDNF expression was increased by swimming for 30 minutes everyday for a week. Therefore, therapeutic exercise contribute to brain plasticity after brain ischemia.

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PAHs Formation Characteristics and Fullerenes $(C_{60},\;C_{70})$ Synthesis in a Low-Pressure $C_6H_6/Ar/O_2$ Flame (저압 $C_6H_6/Ar/O_2$ 화염에서 PAHs 생성 특성 및 플러렌$(C_{60},\;C_{70})$ 합성에 대한 연구)

  • Lee, G.W.;Kim, Y.W.;Hwang, J.;Jrung, J.;Choi, M.
    • Journal of the Korean Society of Combustion
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    • v.7 no.4
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    • pp.36-44
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    • 2002
  • Carbon molecules with closed-cage structures are called fullerenes $(C_{60},\;C_{70})$, whose applications include super-conductors, sensors, catalysts, optical and electronic device, polymer composites, and biological and medical materials. The synthesis of fullerenes has been recently studied with low-pressure benzene/argon/oxygen flames. The formation of fullerene is known as molecular weight growth processes of PAHs (polycyclic aromatic hydrocarbon). This study presents results of PAHs and fullerene measurements performed in a low-pressure benzene/argon/oxygen normal co-flow laminar diffusion flame. Through the central tube of the burner, benzene vapors carried by argon are injected. The benzene vapors are made in a temperature-controlled bubbler. The burner is located in a chamber, equipped with a sampling system for direct collection of condensable species from the flame, and exhausted to a vacuum pump. Samples of the condensable are analyzed by HPLC (High Performance Liquid Chromatography) to determine the yields of PAHs and fullerene. Also, we computed mole fraction of fullerene and PAHs in a nearly sooting low pressure premixed, one-dimensional benzene/argon/oxygen flame (equivalence ratio ${\Phi}=2.4$, pressure=5.33kPa). The object of computation was to investigate the formation mechanism of fullerenes and PAHs. The computations were performed with CHEMKIN/PREMIX. As a result of this study, fullerenes were synthesized in a low pressure (20torr) $C_6H_6/Ar/O_2$ flames and the highest concentration of fullerene was detected just above the visible surface of a flame.

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A Study of Improvement of Dalsung Park Total Design in Daegu City (대구 달성공원의 통합디자인 개선에 관한 연구)

  • 송재은;박규현
    • Archives of design research
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    • v.16 no.4
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    • pp.471-480
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    • 2003
  • Well designed idealistic zoo requires basically a condition that the zoo is much ecologically considered for the exhibited animals, and secondly where they offer leisure for the visitors' convenience and pleasure. But it is not easy to find a zoo where they have actualized these both-sided purposes. We cannot but discuss the problem of design caused by a lack of plan-ability that is necessary when the zoo is designed. It is because every elements are not wholly unified and those diversified contents aren't mutually harmonized. Therefore, a zoo has to meet the requirement of the pleasure, study, inspection and leisure deserves a special emphasis. The zoo requires a synthesized speculation of design which the elements-Identity Program, Furniture System, animal's ecological environment, animal's showroom, cage and facilities etc., - are mutually harmonized. This study is a combined-design research on the Daegu Dal-sung Park in this concept. 1 have newly analyzed the Dal-sung Park's utilizing form in order to harmonize both visual and ecological environments.

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Effect of Xylanase Supplementation on the Net Energy for Production, Performance and Gut Microflora of Broilers Fed Corn/Soy-based Diet

  • Nian, F.;Guo, Y.M.;Ru, Y.J.;Peron, A.;Li, F.D.
    • Asian-Australasian Journal of Animal Sciences
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    • v.24 no.9
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    • pp.1282-1287
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    • 2011
  • The objective of this study was to assess the effect of xylanase on net energy for production, performance, nutrient digestion and gut microflora of broilers fed corn/soy-based diet. Eighty-four day-old male broiler chicks were allocated to two groups receiving two treatments, respectively. Each treatment had six replicate cages with seven broilers per cage. The diets were based on corn and soybean. The treatments were: i) basal diet reduced in apparent metabolizable energy (-0.63 MJ/kg compared to commercial diet specifications); ii) basal diet supplemented xylanase at 4,000 u/kg feed. The experiment used the auto-control, open circuit respiration calorimetry apparatus to examine the heat production and net energy for production. The results revealed that xylanase supplementation did not affect growth performance and diet AME value, but increased $NE_p$ value by 18.2% (p<0.05) and decreased daily heat production per $kg^{0.75}$ by 31.7% (p<0.05). There was no effect (p>0.05) of xylanase supplementation on the ileal digestibility of N and hemicelluloses, but the ileum digestibility of energy was increased by 2% by xylanase supplementation (p<0.05). Xylanase supplementation increased (p<0.05) the count of lactobacillus and bifidobacterial in the caecum.