• Title/Summary/Keyword: CV13

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Effect of Liquid Fertilizer Containing medium of Lactobacillus confusa and Pichia anomala on Growth in Creeping Bentgrass (유산균(Lactobacillus confusa)과 효모균(Pichia anomala) 배양액 함유 액비의 시용이 크리핑 벤트그래스의 생육에 미치는 영향)

  • Kim, Young-Sun;Ham, Suon-Kyu;Kim, Tack-Soo;Jeon, Hyun-Suk
    • Asian Journal of Turfgrass Science
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    • v.22 no.2
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    • pp.185-196
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    • 2008
  • This Study was conducted to evaluate the effect of liquid fertilizer containing medium of on growth of shoot and root in creeping bentgrass (Agrostis palustris Huds. cv. Pennlixs). According to application method of liquid fertilizer, the experiment plot was designed as follows; NF : non-fertilizer; CF : compound fertilizer(21-17-17) only; T500, T300, T100 : compound fertilizer +liquid fertilizer solution diluted 500, 300, 100 folds, respective; L500 : only liquid fertilizer solution which contained the medium of Lactobacillus confusa and Pichia anomala diluted 500 folds. The every treatments was arranged by a completely randomized complete block designs with three replications. The order of turf qualities like leaf color index and chlorophyll content in treatments showed T500 T300 T100 $\geq$ CF > NF. In treatment applied with compound fertilizer, the dry weight of shoot increased by about 13% in T500, T300 and T100 than CF, and that of root about 25% in just T300. Dry weight of shoot and root increased by 88% and 44% in L500 than NF, respectively. As compared with CF, T/R ratio in T500, T300 and T100 increased by 20%, 11% and 21%, respectively and root length 7%, 8% and 3%. In comparison with NF, T/R ratio and root length in L500 increased by 39% and 74%. These results suggested that the application of liquid fertilizer containing medium of Lactobacillus confusa and Pichia anomala induced the development of turf quality and growth by promoting root growth in creeping bentgrass.

Chemical Characteristics, Antimicrobial Activity and Dyeability of Gyeongsanbansi (Persimmon kaki) Unripe Juice Extraction and Fermented Liquor (경산반시의 미숙감 착즙액과 발효액의 화학적 특성, 항균성 및 염색성)

  • Heo, Buk-Gu;Park, Yun-Jum;Kim, Tae-Choon;Kim, Hyun-Ju;Park, Su-Min;Jang, Hong-Gi;Kim, Kyung-Su;Lee, Kyung-Dong;Yun, Jae-Gill
    • Korean Journal of Plant Resources
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    • v.22 no.5
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    • pp.438-445
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    • 2009
  • This study was conducted to examine the chemical characteristics, antibacterial activity and dyeability of several juices made from Persimmon cv. 'Gyeongsanbanshi'. Young fruits of persimmon were harvested at 1st August, 2006. Fruit juice was extracted soon after harvesting, some of them were stored at $4{\sim}6^{\circ}C$ for 6 months or fermented at room temperature for 6 months. $L^*$ values of vinegar was 43.5 higher than those of fresh juice and fermented liquor. Inorganic matter contents in fresh juice, fermented liquor and vinegar were higher in the order of K ($1696{\sim}2880\;mg$/100 g), Ca ($166.7{\sim}417.9\;mg$/100 g), Mg ($203.9{\sim}214.4\;mg$/100 g), P ($37.9{\sim}109.8\;mg$/100 g), Na ($13.2{\sim}23.3\;mg$/100 g) and Fe ($8.4{\sim}14.2\;mg$/100 g). Cotton fabrics dyed with the fermented liquor and vinegar had the largest inhibitory zone against the gram-positive microorganisms with range of $16.0{\sim}35.0\;mm$. Cotton fabrics dyed with the fresh juice showed $9.0{\sim}9.5\;mm$ inhibitory zone against the gram-negative microorganisms, $15.0{\sim}21.0\;mm$ with the juice stored at $4{\sim}6^{\circ}C$ for 6 months, $22.0{\sim}23.0\;mm$ with the fermented liquor and $9.0{\sim}35.0\;mm$ with vinegar. The hue of cotton fabrics dyed with the fermented liquor had a YR levels, and antibacterial activity of them were 78.5%.

Growth of 'Nokkwang' Hot Pepper Plug Seedlings as Influenced by Various Ratios of Pre-planting NH4+:NO3- in Root Substrate (상토에 기비로 혼합된 NH4+:NO3- 비율에 따른 '녹광' 고추 플러그 묘의 생장)

  • Oh, Sang Se;Park, Myong Sun;Kim, Hyun Cheul;Choi, Jong Myung
    • Journal of Bio-Environment Control
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    • v.28 no.2
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    • pp.110-116
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    • 2019
  • This study was conducted to determine the effects of a pre-planting fertilizers with various $NH_4{^+}:NO_3{^-}$ ratios in a coir dust:peatmoss:perlite (3.5:3.5:3.0, v/v/v) medium on the growth of hot pepper (Capsicum annuum L. cv. Nokkwang) plug seedling. Nitrogen levels were fixed to $300mg{\cdot}L^{-1}$ and the $NH_4{^+}:NO_3{^-}$ ratios were varied to 0:100, 27:73, 50:50, 73:27, and 100:0. The 50-cell trays were filled with treatment media containing pre-plant fertilizers, then seeds were sown. After seeds were germinated, the trays were moved to greenhouse and seedlings were feed with 13-2-13 and 20-9-20 fertilizers, alternatively. The changes in pH and EC were measured every week and soil solution for nutrient concentrations were analyzed in week 0, 3, and 7. The measurements of seedling growths as well as analysis of tissue nutrient contents were also conducted in week 7. The varied $NH_4{^+}:NO_3{^-}$ ratios did not influence on the pHs of root media after incorporation of pre-planting fertilizers, but the ECs were heightened as proportion of $NH_4{^+}$ to $NO_3{^-}$ were elevated. During the raising of seedlings, the pHs rose over time in the treatments of 0:100 and 27:73 ($NH_4{^+}:NO_3{^-}$). The concentrations of all macro-elements in root media decreased gradually as seedlings grew in all treatments. The seedling growths 7 weeks after seed sowing were the highest in the treatments of 27:73 and 50:50 ($NH_4{^+}:NO_3{^-}$) and those became worse in the treatments of higher $NH_4{^+}$ ratios than 73%. In terms of inorganic element contents based on the dry weight of above ground tissue, the treatment of 0:100 showed the lowest content of Ca, Mg, Na, Cu, Mn, and Zn. Based on the results, it is desired that $NH_4{^+}$ ratio in pre-planting fertilization is maintained to be 50% or less for the raising of hot pepper plug seedlings.

Study on the Anti-cancer, Anti-metastasis and Immune response improvement of Aqua-acupuncture with Cistanches Herba infusion solution (육종용약침의 항암작용(抗癌作用) 및 면역효과(免疫效果)에 대(對)한 실험적(實驗的) 연구(硏究))

  • Lee, Eun-Yong;Lee, Byung-Ryul
    • Journal of Acupuncture Research
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    • v.17 no.1
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    • pp.251-286
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    • 2000
  • To study the effects of anti-cancer, anti-metastasis and immune response improvement of aqua-acupuncture with Cistanches Herba infusion solution, we used Cistanches Herba infusion solution(taken by water-alcohol method) put into Chung-wan(CV12) and Chok-Samni(S36) of BALB/c or C57BL/6 which are corresponding to human body. We observed the cytotoxicity, the effect on the expression of MMP-9 gene, the ability to control cancer cell proliferation, change of body weight, surviving number, MST, ILS, changes in amount of WBC, RBC, PLT, total protein, creatinine, glucose and LDH, weight of spleen, number of pulmonary colony, histological analysis on tissue metastasis of lung and liver, splenic cell proliferation, the expression of cytokine gene, the number of $CD4^+$, $CD8^+$, $CD19^+$ and NK cell, and concluded like this. The results were obtained as follows : 1. The cytotoxicity about B16-F10 cell line of $2^0$, $2^{-1}$, $2^{-2}$, $2^{-5}$ diluent groups in Cistanches Herba infusion solution treatment was inhibited significantly, compared with control group. 2. The cytotoxicity about HT1080 cell line of $2^0$, $2^{-1}$, $2^{-2}$, $2^{-3}$, $2^{-5}$, $2^{-8}$ diluent groups in Cistanches Herba infusion solution treatment was inhibited significantly, compared with control group. 3. The effect on expression of MMP-9 gene was decreased in all the sample groups, compared with control group. 4. The effect on the control-ability on the cancer cell proliferation showed cytotooicity significantly in $2^0$, $2^{-1}$, $2^{-2}$, $2^{-3}$, $2^{-4}$, $2^{-5}$ diluent groups. 5. S-180 cancer cell line transplants in BALB/c mice were inhibited significantly in weight increase in all the sample groups, compared with control group. The surviving number increased in almost sample groups, except one group put into Chok-Samni(S36) with 20% Cistanches Herba infusion solution treatment group that showed same number of the control group. 6. S-180 cancer cell line transplants in BALB/c mice showed high MST and ILS significantly in almost sample groups, compared with control group. But one group put into Chok-Samni(S36) with 20% Cistanches Herba infusion solution treatment group showed low MST and ILS than control group. 7. The sample group injected in vein with B16-F10 cancer cell line in C57BL/6 mice showed increased ILS compared with control group significantly in anti-metastasis test. 8. The sample group injected in vein with B16-F10 cancer cell line in C57BL/6 mice were increased significantly in the number of WBC and glucose, and decreased significantly in the amount of LDH, compared with control group. However, there's no significant increase or decrease in number of RBC, PLT, total protein and creatinine. 9. We couldn't find any significant relation in spleen weight of the sample group. 10. In pulmonary colony, sample group was decreased significantly, compared with control group. 11. Histological analysis of sample group inhivited compared with that of control group in both of lung and liver. 12. In immune system, all the sample groups showed having more relevancy to the effect on splenic cell proliferation than normal group. 13. The effect on cytokine gene expression of all the sample groups were increased than control group. 14. In flow cytometry there's no significant relation in number of $CD8^+$, $CD19^+$ cell, however, the number of $CD4^+$ cell and NK cell in sample groups were increased than in control group. Above the results showed that aqua-acupuncture of Cistanches Herba infusion solution has effects of anti-cancer, anti-metastasis and immune response improvement.

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The Influence of Traffic Time and Fertilizer Type on the Quality of Golf Course Putting Greens (답압시간과 비료종류가 골프코스 그린 잔디의 품질에 미치는 영향)

  • Lee, Sung-Woo;Lee, Jae-Pil;Kim, Doo-Hwan
    • Asian Journal of Turfgrass Science
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    • v.22 no.1
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    • pp.65-74
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    • 2008
  • Traffic management is becoming an important issue in turfgrass practise on golf course. The objective of this study was to investigate the combined impact of different traffic times and types of fertilization. Traffic treatment was applied in morning (AM), noon, and afternoon (PM). Fertilizers used include faster-release fertilizer (21-17-17) and slow-release fertilizers (12-6-18, 11-3-22, 20-3-20, 10-3-10). Experiment was conducted from Oct. 1 to Nov. 30, 2005 on a nursery putting green of Incheon Grand Golf Club. The growth and quality of creeping bentgrass (Agrostis polustris cv. 'Seaside II') were evaluated on visual leaf color, leaf texture, shoot density, and root length. The measurement at 20 days after treatment, turfgrass color and leaf texture showed the best result on 10-15-10 + Noon-traffic plot. Turf quality and traffic tolerance were not different at Am and Pm traffic treatment. However, traffic stress in early morning and late evening caused the most severe damage to the turfgrass. Shoot density was the highest in 10-15-10 + Noon-traffic treatment but root length was not different among treatments after 30 day measurement. Among the fertilizers, slow release fertilizer resulted in higher turf quality and traffic tolerance than fast release fertilizer, however, shoot density did not showed a significantly different. For the fast recuperation of turfgrass from traffic injury in the early winter, it is recommended to avoid early morning and late evening traffic such as cup replacement and other maintenance practise. It is also recommended to delay the first tee-up time and ending early for last tee time during cold weather season.

Studies on Synthesis and Accumulation Pattern of Cyannogenic Glycosides in Sorghum Piants (Sorghum 식물에 있어서 Cyanogenic Glycosides의 합성 및 축적에 관한 연구)

  • ;G. Voigtlaender
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.5 no.2
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    • pp.121-126
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    • 1985
  • Phytotron and field experiments were conducted to determine the influence of morphological growth stage and environmental temperature on synthesis and accumulation pattern of cyanogenic glycosides in sorghum cv. Pioneer 931 and Sioux at Munich technical university from 1979 to 1980. Various growth stages of sorghum plants were grown in phytotron at 4 different temperature regimes of 30/25, 25/20, 28/18 and 18/8 degree C with 35,000 Lux over 13-h days. The results obtained are summarized as follows: 1. Cyanogenic glycosides in sorghum plants were shown to have a great synthetic rate at early growth stages. The highest concentrations of hydrocyanic acid (HCN) were found at 2-leaf stage with 2384 and 1800ppm (DM basis) for Pioneer 931 and Sioux respectively. The contents of HCN were, however, however decreased markedly as morphological development, which shows a value of 173ppm (Pioneer 931) and 70ppm (Sioux) at heading stages. 2. Changes of hydrocyanic acid in sorghum plants were positive correlated with leaf weight ratio and leaf area ratio ($P{\leqq}0.1%$), while plant height shows a negative correlation with HCN contents ($P{\leqq}0.1%$). 3. Cyanogenic glycosides were accumulated in young plants mainly in leaves. During the late maturities, the contents of HCN in leaves and stalks were shown, however, a similar distribution. 4. Synthesis rates of cyanogenic glycosides were increased under high temperature. Accumulated hydrocyanic acid in the plants was, however declined when temperature exceeded 30 degree C. 5. Synthesis rates of cyanogenic glycosides were affected by nitrogen reductase activity (NRA). The concentration of hydrocyanic acid in sorghum plants was associated with increasing of nitrate-N accumulation.

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Interactions between Oxidative Pentose Phosphate Pathway and Enzymes of Nitrate Assimilation "Nitrate Reductase, Nitrite Reductase, Glutamine Synthetase$_1$" and Ammonium Reassimilation "Glutamine Synthetase$_2$" as affected by $No_3$-Concentration ($No_3$-수준이 Oxidative Pentose Phosphate Pathway와 질산동화작용 효소"Nitrate Reductase, Nitrite Reductase, Glutamine Synthetase$_1$" 및 암모늄재동화작용 주요효소"Glutamine Synthetase$_2$"활성도의 상호관계에 미치는 영향)

  • Sohn, Sang-Mok;Michael James Emes
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.37 no.5
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    • pp.468-475
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    • 1992
  • In order to understand more clearly the integration between N-assmilation and C-metabolism in relation to N fertilization, a pot experiment with 5 different level of N fertilization(0, 5, 10, 25, 50 mM NO$_3$$_{[-10]}$ ) was conducted in Manchester, U.K. The peas (Pisum sativum L., cv. Early Onward) were sown in vermiculate (5 cm depth) and cultivated for 6 days under temperature controlled dark room conditions ($25^{\circ}C$). The plants received frequent irrigation with a nutrient solution: it was fertilized every 2 days, 3 times a day at 10h, 13h, 16h respectively. Elevated NO$_3$$^{[-10]}$ concentration, the activity levels of NR, NiR, total GS(crude extract), GS$_2$(plastid) in both root and shoot were increased and reached the peak in 5~25 mM, except NiR specific activity which increased its activity continually until 50 mM NO$_3$$^{[-10]}$ treatment. Total activities of GS (crude extract) in both root and shoot became higher than those of GS$_2$(Plastid), and the activity ratios of total GS in the crude extract and GS$_2$ in the plastids were 3.0 to 4.3 in root, but 3.2 to 10.6 in shoot. It was concluded that the reductants and A TP from OPPP itself should be enough to achieve the high rate of NR, NiR, GS$_1$, GS$_2$ in plant root and shoot for reduction or assimilation of nitrogen, but these enzyme activities might be inhibited by an excess of NO$_3$$^{[-10]}$ influx over the reduction capacity.

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The Characteristics of Anatomical Structure and Fruit Quality According to Fruit Developmental Stage of Pyrus pyrifolia Nakai cv. Manpungbae ('만풍배'의 생육기별 해부학적 구조와 과실품질)

  • Park, Ji-Eun;Kwon, YongHee;Lee, ByulHaNa;Park, YoSup;Jung, MyungHee;Choi, Jin-Ho;Park, Hee-Seung
    • Horticultural Science & Technology
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    • v.31 no.4
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    • pp.407-414
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    • 2013
  • This study was carried out to understand the physiological characteristics of 'Manpungbae' (Pyrus pyrifolia Nakai) pears through the seasonal changes of pericarp structure and anatomical differences between bagging and non-bagging treatment, and also fruit quality and peel coloration characteristics at the harvest time. The pericarp at full bloom was consists of outer epidermis, hypodermis, parenchyma cell, and inner epidermis from the exterior. The cell layers from the outer epidermis to vascular bundle increased rapidly 7-10 layers to 18-26 layers from full bloom (FB) to 77 days after full bloom (DAFB) and did not change significantly until maturity. Thus, the cell division period of 'Manpungbae' pear was until 77 DAFB and during this period, the thickness from hypodermis to vascular bundle increased from $73.1{\mu}m$ to $195{\mu}m$ in this period. Stone cells were formed from seven to 21 DAFB and stone cell clusters were formed around 49 DAFB. The cork cell layer was formed between 49 and 77 DAFB. 'Manpungbae' fruit pericarp was consists of 4.5 layers of the cork cell layers and seven layers of hypodermis which has the tannin at harvest time (161 DAFB). Comparison of the fruit enlargement and fruit structure development by bagging or non-bagging showed that 'Manpungbae' fruits without bagging had more than three cork cell layer than those with bagging at maturity. The size of stone cell clusters were varied in two treatments. Fruit weight was higher in the non-bagging treatment but there was no difference in soluble solid contents (SSC) between two treatments. The weight of the 'Manpunbae' fruit was distributed from 301 g to more 900 g and the average fruit weight was 677.2 g at harvest time, and fruits in the range of 551-800 g accounted for 71.6% of total production. The SSC, acidity and SSC/acidity ratio was $10.2-12.1^{\circ}Brix$, 0.10-1.24% and 9.76-14.31 respectively, and the SSC was higher in bigger fruit which had a very higher positive correlation with a fruit weight. However, the fruit firmness tended to be lower with fruit size which had a very higher negative correlation with the fruit weight and SSC. The cork cell layer numbers between yellowish brown and green pericarp were not different significantly, in 3.8 and 3.5 respectively.

Physicochemical Characteristics of the Sorghum(Sorghum bicolor L. Moench) Powder following Low Temperature-Microparticulation (저온초미분쇄에 따른 수수가루의 이화학적 특성)

  • Kim, Hyun-Young;Seo, Hye-In;Ko, Jee-Yeon;Kim, Jung-In;Lee, Jae-Saeng;Song, Seuk-Bo;Jung, Tae-Wook;Kim, Ki-Young;Kwak, Do-Yeon;Oh, In-Seok;Jeong, Heon-Sang;Woo, Koan-Sik
    • The Korean Journal of Food And Nutrition
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    • v.25 no.3
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    • pp.656-663
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    • 2012
  • Two sorghum(Sorghum bicolor L. Moench cv. Hwanggeumchal-susu and Miryang 3) samples were milled using different milling methods, and their physicochemical properties were tested. Particle size was classified into five groups such as pin mill and low temperature-microparticulation(LTM; 10,000, 20,000, 30,000, and 40,000 rpm). The water absorption index (WAI), water solubility index(WSI), and a rapid Visco analyzer(RVA) were used to examine particle size distribution and color differences. Particle size of sorghum flour prepared using LTM was lower than that prepared using a pin mill. Particle size was further reduced by successive dry milling of the LTM flour. Lightness of colored pigments increased when particle size decreased. The WAI of Miryang 3 pin milling(M1) flour was the lowest after LTM, and WSI was higher in the order of M2, M3, M4, and M5. LTM sorghum flour had significantly higher pasting viscosity, as determined using a rapid Visco analyzer. LTM Miryang 3 sorghum flour(M2~M5) flour showed lower breakdown viscosity and higher final viscosity compared to those of M1 flour, resulting in an increased setback value.

Differential Sensitivity of Rice Cultivars to HPPD-Inhibiting Herbicides and their Influences on Rice Yield (HPPD 억제 제초제에 대한 벼 생태형간 약해 차이 및 수량 영향)

  • Kim, Sang-Yeol;Oh, Seong-Hwan;Lee, Ji-Yoon;Yeo, Un-Sang;Lee, Jong-Hee;Cho, Jun-Hyeon;Song, You-Chun;Oh, Myung-Kyu;Han, Sang-Ik;Seo, Woo-Duck;Jang, Ki-Chang;Na, Ji-Eun;Park, Sung-Tae;Nam, Min-Hee
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.57 no.2
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    • pp.160-165
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    • 2012
  • A field study was conducted at Miryang in 2011 to evaluate differential sensitivity of six rice cultivars, three Indica${\times}$Japonica rice (long grain, cv. Hanareumbyeo, Hanareum2, Dasanbyeo) and three Japonica rice (short grain, Nampyung, Ilpumbyeo and Junamjosaeng) cultivars, to benzobicyclon, mesotrione+pretilachlor (MP) and butachlor (control) in transplanting rice and their treatment influences on rice yield. All Indica${\times}$Japonica rice cultivars exhibited susceptible to both benzobicyclon and MP as reflected by higher visual injury and carotenoid biosynthesis inhibition when compared with Japonica rice cutlivars. The percentage of foliar chlorosis of Indica${\times}$Japonica rice was only 0.9~4.7% for benzobicyclon at 13 days after treatment (DAT) and 30.3~64.4% for MP at 5DAT but it increased rapidly to 88.4~91.2% at 12~20DAT. However, most of the Japonica cultivars are tolerant to bezobicyclon and MP. There was no visible leaf chlorosis but carotenoid biosynthesis was slightly inhibited. Based on relative carotenoid content reduction of benzobicyclon and MP to butachlor, the Indica${\times}$Japonica rice showed 4.6~15.6 fold higher compared with the Japonica rice. The heading date of the injured rice plant was delayed by 3 days and panicle number per square meter and ripened grain ratio were reduced as compared with the control treatment of butachlor. Rice yield of the Indica${\times}$Japonica rice cultivars treated with benzobicyclon and MP was reduced by 7~10%, 3~5%. respectively. The result indicates that rice cultivars vary in tolerance to HPPD-Inhibiting herbicides and Indica${\times}$Japonica rices were more susceptible than the Japonica rices to MP and benzobicyclon. Rice yield of the Indica${\times}$Japonica rices was also significantly reduced by the those herbicide treatments.