• 생물정신의학
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• 제4권1호
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• pp.48-53
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• 1997

CV(bDAT) cell line, expressing dopamine transporter stably, has been established by transfection of CV-1 cells with bovine dopamine transporter cDNA. Using CV(bDAT) cells, the effects of various antipsychotic drugs on dopamine uptake activity were investigated. All of antipsychotic drugs tested, inhibited the [$^3H$]dopamine uptake into CV(bDAT) cells with $IC_{50}s$ in the low to mid micromolar range, implying that antipsychotic drugs may produce overflow of dopamine in the synaptic cleft of dopaminergic neuron.

• BMB Reports
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• 제28권3호
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• pp.216-220
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• 1995

cDNA for human cholesteryl ester transfer protein (CETP), a potent atherogenic plasma protein that redistributes the neutral lipids among lipoproteins, was expressed in recombinant vaccinia virus-infected cells (CV-1). Two insertion vectors regulated by different promoters were constructed. The vectors were introduced into human thymidine kinase-negative ($TK^-$) 1438 cells infected with wild-type vaccinia virus (WR strain). Recombinant viruses were selected with 5-bromodeoxyuridine (BUdR) and X-gal and identified with DNA dot blot analysis (vSC11-CETP and vTM1-CETP). The CETP cDNA insert in the recombinant vaccinia virus genome was identified by Southern blot analysis. Transcription of CETP cDNA in CV-1 cells infected with recombinant vaccinia virus was monitored by Northern blot analysis using the CETP cDNA as a probe. Positive signals were detected at 1.8 kb in cells infected with vSC11-CETP and at 2.3 kb in cells infected with vTM1-CETP. The recombinant vaccinia virus-infected CV-1 cells were shown to produce functional CETP when the culture medium was subjected to the CETP assay.

• 약학회지
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• 제39권2호
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• pp.161-168
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• 1995

Brain dopamine systems play a central role in the control of movement, hormone release, and many complex behavior. The action of dopamine at its synapse is terminated predominately by high affinity reuptake into presynaptic terminals by dopamine transporter (DAT). The dopamine transporter(DAT) is membrane protein localized to dopamine-containing nerve terminals and closely related with cocaine abuse, Parkinsonism, and schizophrenia. In present study, the recombinant plasmid pRc/CMV-DAT, constructed by subcloning of a cDNA encoding a bovine DAT into eukaryotic expression vector pRc/CMV, was stably transfected into CV-1 cells(monkey kidney cell line). The DAT activities in the cell lines selected by Geneticin$^{R}$ were determined by measuring the uptake of $[^3H]$-dopamine. The transfected cell lines showed 30-50 fold higher activities than untransfected CV-1 cell line, and this result implies that DAT is well expressed and localized in transfected cells. The transfected cells accumulated $[^3H]$-dopamine in a dose-dependent manner with a $K_{m}$ of 991.6nM. Even though high doses of norepinephrine, epinephrine, serotonin, and choline neurotransmitters inhibited the uptake of $[^3H]$-dopamine, DAT in transfected cell line was proven to be much more specific to dopamine. The psychotropic drugs such as GBR12909, CFT, normifensine, clomipramine, desipramine, and imipramine inhibited significantly the dopamine uptake in tissue culture cells stably transfected with DAT cDNA. Radioactive in situ hybridization was done to map the cellular localization of DAT mRNA-containing cells in the adult rat central nervous system. The strong hybridization signals were detected only in the substantia nigra pars compacta and ventral tegmental area. The restricted anatomical localization of DAT mRNA-containing cells confirms the DAT as a presynaptic marker of dopamine-containing cells in the rat brain.

• 한국가정과학회지
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• 제7권3호
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• pp.49-58
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• 2004

The purpose of the study was to verify if or not as funtional food by estimating antitumor activity and antimicrobial activity of Coriolus versicolor(Fr.)Quel and Ganoderma Lucidum (Fr.)Karst. 1. The contents of moisture. crude fiber and crude protein of GL were higher $(18.28\%,\;10.3\%,\;78.4\%)$ than that of CV, but the content of crude lipid GL was higher than that of CV. 2. Inhibitory effects of on the growth of AGS human gastric adenocarcinoma cells and HT-29 human colon adenocarcinoma cell were increased by increasing of concentration in added methanol extracts of CV and GL. 3. Antimicrobial activity on Escherichia col1; Staphylococcus aureus, Salmonella typhimurium existed methanol extracts of CV. Therefore. it is suggested that CV is worth as functional food.

• 한국미생물·생명공학회지
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• 제43권2호
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• pp.126-133
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• 2015

본 연구에서는 정치 배양법으로 고농도의 초산을 생산할 수 있고 에탄올 내성이 우수한 균주를 확보하고자 농가형 발효식초에서 초산균을 분리 및 선발하였고, 이들 초산균의 형태적 특징을 조사한 바, 분리균 CV1은 그람 음성으로 운동성이 없는 간균으로 나타났다. 분리균의 chemotaxonomy를 분석한 결과, meso-DAP이며, 대표 퀴논은 Q₁₀이고, G+C mol 함량은 61.0 mol %로 나타났으며 16S rDNA 유전자의 염기서열을 분석한 결과, Gluconacetobacter saccharivorans로 동정되어 Glu. saccharivorans CV1로 명명하였다. CV1 초산균의 최적 성장조건은 30℃, pH 3.0 이상으로 판단되었고 에탄올 농도에 따른 초산 생성능은 10% 에탄올 농도에서 9.3%, 9% 에탄올 농도에서는 8.4% 적정산도를 나타내어 고농도 에탄올 조건에서도 높은 산 생성능을 나타내는 우수한 균주로 판단되었다.

• Bulletin of the Korean Chemical Society
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• 제28권4호
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• pp.515-519
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• 2007

Neurotransmitter glycine in the nano gram range was analyzed using a paste electrode (PE) in cyclic voltammetry (CV) and square-wave stripping voltammetry (SWSV). An anodic peak caused by oxidation of the glycine ion appeared at the 0.4 V (versus Ag/AgCl/KCl) potential in a 0.1 M NH4H2PO4 electrolyte solution. At optimized conditions, the working range of the SWSV and CV concentration was found to be 5-60 ngL-1 glycine; precision of R2 = 0.9816 (SWSV) and 0.9986 (CV); and detection limit of 0.65 ngL-1 (5.82 × 10-12 molL-1) (S/N = 3). The optimized conditions were applied to an assay in a fish brain tissue and a living brain cell in real time.

• Journal of Acupuncture Research
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• 제21권5호
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• pp.205-218
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• 2004

Objectives : The purpose of this experiment is to study on the anti-cancer, anti-metastasis and immune response improvement effects of Herbal-acupuncture with Carthami Flos infusion solution(CTT-HAS). Methods : We injected Carthami Flos infusion solution into Chung-wan(CV12) of C57BL/6 mouse which is corresponding to human Chung-wan(CV12). We observed its effect on the number of $CD25^{+}/CD4^{+}$, $CD8^{+}/CD3e^{+}$, $CD69^{+}/B220^{+}$, $NK^{+}/CD3e^{+}$ cells in mouse PBMCs, the number of the pulmonary colony, and the effect on MST and ILS of C57BL/6 mice implanted intravenously with B16-F10 melanoma. Results Conclusions : 1. The spleen cells proliferation of the sample groups treated with CTT-HAS extract has increased significantly compared with that of the control group. 2. The percentage of the $CD25^{+}/CD4^{+}$, $CD8^{+}/CD3e^{+}$, $CD69^{+}/B220^{+}$, $NK^{+}/CD3e^{+}$ cells in C57BL/6 mouse PBMCs of the sample groups treated with CTT herbal-acupuncture has increased compared with that of the control group. 3. The lung colony number of the sample groups CTT Herbal-acupuncture has decreased significantly compared with that of the control group. 4. MST and ILS of the sample groups CTT herbal-acupuncture have increased significantly compared with those of the control group.

• Journal of Acupuncture Research
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• 제21권6호
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• pp.85-102
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• 2004

Objective : The purpose of this experiment is to study on the anti-cancer, anti-metastasis and immune response improvement effects of Herbal-acupuncture with Sinomenii acuti lignun infusion solution(SAL-HAS). Methods : We injected Sinomenii acuti Lignum infusion solution into Chung-wan(CV12) of C57BL/6 mouse which is corresponding to human Chung-wan(CV12). We observed its effect on the nunter of $CD25^+/CD4^+$, $CD8^+/CD3e^+$, $CD69^+/B220^+$, $NK^+/CD3e^+$ cells in mouse PBMCs, the number of the pulmonary colony, and the effect on MST and ILs of C57BL/6 mice implanted intravenously with B16-F10 melanoma. Results & Conclusions : 1. The spleen cells proliferation of the sample groups treated with SAL-HAS extract has increased significantly compared with that of the control group. 2. The percentage of the $CD25^+/CD4^+$, $CD8^+/CD3e^+$, $CD69^+/B220^+$, $NK^+/CD3e^+$ cells in C57BL/6 mouse PBMCs of the sample groups treated with SAL herbal-acupuncture has increased compared with that of the control group. 3. The lung colony number of the sample groups SAL Herbal-acupuncture has decreased significantly compared with that of the control group. 4. MST and ILS of the sample groups SAL herbal-acupuncture have increased significantly compared with those of the control group.

• 한국식품조리과학회지
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• 제21권2호
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• pp.243-249
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• 2005

제주산 감귤과즙을 이용하여 감귤식초를 제조할 목적으로 식초산 발효균 CV1과 CV2를 분리하고 식초산 생성능력을 산업적으로 이용되고 있는 균주와 비교 검정하고 165 rDNA 염기서열을 분석하고 동정하였다. 분리균은 8일간의 발효에 의하여 산도 $5.5\%$ 이상의 식초산을 생성하였으며, 감귤식초 발효에는 사과식초나 감식초 발효에 이용되고 있는 균주 보다 더욱 적합한 것으로 판정되었다. 분리한 균주는 Gluconacetobacter hanenii(CV2) 및 G. hanenii의 변이주(CV1)로 동정되었다.

• 한국식품과학회지
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• 제49권5호
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• pp.538-543
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• 2017

본 연구에서는 떫은 감인 청도반시(Diospyros kaki Thunb. cv. Cheongdo-Bansi)를 탈삽하여 곶감으로 만드는 과정 중에 다량의 부산물로 생겨나는 감 껍질을 활용하고자 산화방지능 및 신경세포 보호능을 평가하였다. 탈삽한 감 껍질을 40% (v/v) 에탄올-물혼합용액을 사용하여 초음파 추출을 한 후에, 가열처리와 비가열처리 방법을 이용하여 각각의 추출물을 확보하였다. 가열처리한 추출물이 비가열처리로 얻어진 것에 비해서 총페놀 함량, 총플라보노이드 함량, 산화방지능에서 약 1.3-1.8배 더 높았다. 비가열처리 추출물과 비교하여, 가열처리한 것이 PC-12 신경세포의 생존율 더 높이고, 세포 내 산화스트레스를 좀 더 완화하였다. AChE와 BChE 억제능 역시 가열처리한 추출물이 비가열처리로 얻은 추출물보다 더 높았다. 이러한 결과들은 산화방지능을 보유하며 콜린가수분해효소를 억제하는 기능성 소재 발굴을 위한 탈삽 감껍질 추출물 활용 가능성을 보여준다.