• Carbon letters
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• v.9 no.3
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• pp.203-209
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• 2008

The objective of this work is to study the feasibility of the preparation of the activated carbon (AC) from coconut tree flowers using high temperature fluidized bed reactor (HTFBR). The activating agent used in this work is steam. The reactor was operated at various activation temperature (650, 700, 750, 800 and $850^{\circ}C$) and activation time (30, 60, 120 and 240 min) for the production of AC from coconut tree flowers. Effect of activation time and activation temperature on the quality of the AC preparation was observed. Prepared AC was characterized in-terms of iodine number, methylene blue number, methyl violet number, ethylene glycol mono ethyl ether (EGME) surface area and SEM photographs. The best quality of AC from coconut tree flowers (CFC) was obtained at an activation temperature and time of $850^{\circ}C$ and 1 hr restectively. The effectiveness of carbon prepared from coconut tree flowers in adsorbing crystal violet from aqueous solution has been studied as a function of agitation time, carbon dosage, and pH. The adsorption of crystal violet onto AC followed second order kinetic model. Adsorption data were modeled using both Langmuir and Freundlich classical adsorption isotherms. The adsorption capacity $q_m$ was 277.78 mg/g., equilibrium time was found to be 180 min. This adsorbent from coconut tree flowers was found to be effective for the removal of CV dye.

• Journal of the Microelectronics and Packaging Society
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• v.6 no.2
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• pp.69-74
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• 1999

Graphite and carbonaceous materials intercalate and deintercalate Li-ion reversibly into their layered structures. These materials show an excellent capacity for using a negative electrode in Li-ion batteries, because the electrochemical potential of Li-ion intercalated carbon is almost identical with that of lithium metal. Carbon used in this study was obtained by the pyrolysis of petroleum pitch, and heat-treated at the several temperatures between $700^{\circ}C$ and $1300^{\circ}C$. XRD analysis revealed that crystallization of carbon increased with increasing the heat treatment temperature. Charge/discharge properties were studied by a constant-current step at the rate of 0.1C, and the interfacial reaction between the electrolyte and the surface of carbon electrode was studied by cyclic voltammetry. Cell capacities were investigated in terms of the heat treatment temperature and the cycle number. Reversible capacity increased with the heat treatment temperature up to $1000^{\circ}C$, thereafter decreased continuously. Also, charge capacity decreased with the cycle number, while the reversibility improved with it.

• Journal of Plant Biotechnology
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• v.5 no.2
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• pp.87-93
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• 2003

Brachiaria (Poaceae) is the most important forage genus for cattle production in Brazil. The genetic breeding of this genus is limited by the incompatibility among species, differences in ploidy level and the natural cloning of plants by apomixis (Valle and Miles 1992). However, plant regeneration via tissue culture methods and genetic engineering provide an opportunity to introduce new characteristics in plants of this genus. We have developed methods for the 'genetic modification of Brachiaria brizantha cv. Marandu via biolistic transformation. A higher number of shoots was obtained with 4 mg/L 2.4-diclorophenoxyacetic acid and 0.2 mg/L benzylaminopurine in calli induction medium and 0.1 mg/L naphtaleneacetic acid and 4.0 mg/L kinetin in shoot regeneration medium. A selection curve for mannose was determined to use phospho mannose isomerase (PMI) gene of Escherichia coli as a selection marker. Calli formation was inhibited from 5 g/L mannose, even in the presence of sucrose while calli that were formed in the presence of mannose failed to develop embryos showing that PMI gene can be used for selection of transformants of this grass. Different promoters were tested to evaluate the efficiency based on the detection of the GUS gene expression (Jefferson et al. 1987). The monocot promoters, act1-D and ubi-1, resulted in higher expression levels than dicot promoters, ubi-3 and act-2, or the CaMV35S and CVMV promoters.

• Journal of Photoscience
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• v.12 no.1
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• pp.1-9
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• 2005

The ubiquitin E3 ligase COP1 (Constitutive Photomorphogenesis 1) is a protein repressor of photomorphogenesis in Arabidopsisplants, and it found in various organisms, including animals. The COP1 protein regulates the stability of many of the light-signaling components that are involved in photomorphogenesis and in the developmental processes. To study the effect of COP1 on flowering in a short day plant, we have cloned a full-length of PnCOP1 (Pharbitis nil COP1) cDNA from Pharbitis nil Choisy cv. Violet, and we examined its transcript levels under various conditions. A full-length PnCOP1 cDNA consists of 2,280 bp nucleotidesthat contain 47 bp of 5'-UTR, 232 bp of 3'-UTR including the poly (A) tail, and 1,998 bp of the coding sequence. The deduced amino acid sequence contains 666 amino acids, giving it a theoretical molecular weight of 75 kD and a isolectric point of 6.2. The PnCOP1 contains three distinct domains, an N-terminal $Zn^2+$-binding RING-finger domain, a coiled-coil structure, and WD40 repeats at the C-terminal, implying that the protein plays a role in protein-protein interactions. The PnCOP1 transcript was detected in the cotyledon, hypocotyls and leaves, but not in root. The levels of the PnCOP1 transcript were reduced in leaves that were a farther distance away from the cotyledons. The expression level of the PnCOP1 gene was inhibited by light, while the expression was increased in the dark. During the floral inductive 16 hour-dark period for Pharbitis nil, the expression was increased and it reached its maximum at the 12th hour of the dark period. The levels of PnCOP1 mRNA were dramatically reduced upon light illumination. These results suggest that PnCOP1 may play an important function in the floral induction of Pharbitis nil.

• Korean Journal of Acupuncture
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• v.37 no.3
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• pp.203-208
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• 2020

Objectives : Since there are complex associations between diseases/symptoms and acupoints, one-to-one correspondence may not be the proper approach. Pattern identification has been being used as a clinical framework to make treatment decisions by extracting and synthesizing clinical data including patients' signs and symptoms. In this article, we propose two different models explaining the relationships between diseases and acupoints based on the branch treatment [Zhibiaofa] and the root treatment [Zhibenfa]. Methods : We explained the relationships between diseases/symptoms and acupoints from the example data from our previous study on traditional acupuncture point selection patterns for pain control. Diseases include low back pain, migraine, irritable bowel syndrome, osteoarthritis, ankle sprain, carpal tunnel syndrome, and dysmenorrhea, and acupoints included LI4, BL23, BL25, SP6, BL60, TE5, and CV4. Results : The relationships between diseases/symptoms and acupoints can be explained directly based on the branch treatment, and also can be explained indirectly through pattern identification based on the root treatment. Pattern identifications included both meridian-based pattern identification based on the spatial information of diseases and visceral organ-based pattern identification based on the characteristics of diseases. Conclusions : In the East Asian traditional medicine, Korean medicine doctors choose the most appropriate acupoints based either on the diseases/symptoms (i.e., branch treatment) or on the results of pattern identifications (i.e., root treatment). It is necessary to understand the two different approaches to choose specific acupoints for the targeted diseases.

• FLOWER RESEARCH JOURNAL
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• v.16 no.4
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• pp.255-259
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• 2008

This study was conducted to investigate the effect of ridge height (10 cm, 20 cm, 30 cm, and 40 cm) on the soil physical property, the growth and flowering characteristics of Gypsophila paniculata (cv 'Bristol Fairy') in the subalpine area. As ridge height got higher, the daily soil temperature, the soil moisture content, soil hardness, bulk density, and solid phase ratio were decreased, but porosity ratio was a little increased. In summer and autumn cultivation the blooming of ridge height 30 cm and 40 cm was slightly delayed, but their root activity, cut flower width, and yield were better than those of 10 to 20 cm ridges. And the mortality ratio of plants, rosette formation rate, and nonpaniculata inflorescence rate became lower.

• Plant Resources
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• v.2 no.1
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• pp.26-30
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• 1999

Nitrate in edible vegetables is converted to nitrite by nitrate reductase(NR) and/or bacteria in intestines. Nitrite and amino, in the intestine of some animals and human, bind to form nitrosamine, which is toxic and known as carcinogen. This study was carried out to examine the effect of added chlorides and their concentrations on growth, yield and nitrate content in leaf lettuce plants in hydroponics. Seeds of lettuce cv, "Samsunjokchukmyon" were planted on April 29, and seedlings were planted on June 2, and were cultured until July 5 in 1998. KCI and CaCl₂ were used as chloride source and their concentrations were 1, 2 and 4 me/L, respectively, in the lettuce standard nutrient solution for National Horticultural Research Institute(NHRI). Completely randomized design with 3 replications was used. Nitrate content and NR activity were measured 2 and 5 weeks after planting(WAP). The obtained results were summarized as the follows : Leaf weight per plant was difference from harvest dates and treatments, but total leaf weight was not significantly different among treatments. Number of leaves was higher in KCI 2 me/L, CaCl₂1 me/L and control at 2 WAP than the others, and was higher in KCI 1 me/L, and control at 3 WAP than others, and was higher in control at 5 WAP. Total number of harvested leaves was the highest in control with 14, which followed by KCI 2 me/L and CaCl₂1 me/L. Nitrate content was decreased by addition of chloride in nutrient solution. Nitrate content in the 3rd and 9th leaves was significantly decreased. NR activity was higher in control and CaCl₂ addition treatments, while KCI addition treatments reduced NR activity. However, no direct relationship with nitrate was observed. Growth characteristics such leaf length and leaf width were not significantly influenced by chloride addition.

• Research in Plant Disease
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• v.24 no.4
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• pp.321-327
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• 2018

In 2018, symptoms of black rot on apple (Malus pumila var. dulcissima KOIDZ) cv. Fuji were observed in Yeongcheon-si, Korea. The fruit decay symptoms consisted of purple pimples spots, black rot around the seed cavity (calyx end), mummified fruit. To isolate the causal agent, small fragment (2 to 3 mm) of decayed tissue from the lesion margin were placed on WA or PDA. Fungal colonies on PDA produced dense white aerial mycelium, becoming dark gray with age. Pycnidia and conidia were observed under a light microscopy. The shapes of conidia were aseptate, ovoid, rounded at both ends, and $21.7-28.3{\times}9.9-15.3{\mu}m$. Based on morphological and cultural characteristics, this fungus was identified as Diplodia seriata. To confirm its identity, two loci (ITS and ${\beta}$-tubulin) were sequenced for molecular identification. BLAST searches indicated 100% identity with D. seriata. A pathogenicity test was conducted with isolates on Fuji apples. The apples were inoculated with mycelial plugs (5 mm) from 7-day-old cultures of the putative pathogens. All inoculated apples developed rot symptoms identical to the original symptoms, from which D. seriata were reisolated, fulfilling Koch's postulates. This study is the first report of black rot caused Diplodia seriata on apple.

• Phonetics and Speech Sciences
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• v.10 no.4
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• pp.127-134
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• 2018

This study aims to explain the difficulties children with articulation and phonological disorders have in producing alveolar fricative sounds. The study will perform a comparative analysis revealing how ordinary children produce alveolar fricative sounds through five different acoustic variables, and consequently identifying objective differences, compared to children with articulation and phonological disorders. Therefore, this study compared and analyzed the differences between 10 children with articulation and phonological disorders and 10 ordinary children according to a phonation type of alveolar fricative sounds (/s/ and /$s^*$), a type of vowel (/i/, /ε/, /u/, /o/, /ɯ/, /ʌ/, /ɑ/), and a structure of syllables (CV, VCV) through acoustic variables including a central moment, skewness, kurtosis, a center of gravity and variance. That is, children with articulation and phonological disorders, when compared to ordinary children, have difficulties with concentrating an agile and momentary friction with strength when articulating alveolar fricative sounds, which uses strong energy and accompany tension. Furthermore, the values of alveolar fricative sounds of children with articulation and phonological disorders appeared to spread evenly over the average range, which means that the range of overall the standard deviation values for children with functional phonological disorders is wider than that of ordinary children. For a future study, if the mispronounced sounds relating to omission, substitution, and addition can be compared and analyzed for various target groups, it could be used effectively to help children with functional phonological disorders.

• Korean Chemical Engineering Research
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• v.57 no.5
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• pp.606-610
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• 2019

In this study, we demonstrated that the nonenzymatic glucose sensor based on the flexible carbon fiber bundle electrode with BDD nanocomposites (CF-BDD electrode). As a nano seeding method for the deposition of BDD on flexible carbon fiber, electrostatic self-assembly technique was employed. Surface morphology of BDD coated carbon fiber electrode was observed by scanning electron microscopy. And the electrochemical characteristics were investigated by cyclic voltammetry, electrochemical impedance spectroscopy and chronoamperometry. This CF-BDD electrode exhibited a large surface area, a direct electron transfer between the redox species and the electrode surface and a high catalytic activity, resulting in a wider linear range (3.75~50 mM), a faster response time (within 3 s) and a higher sensitivity (388.8 nA/mM) in comparison to a bare CF electrode. As a durable and flexible electrochemical sensing electrode, this brand new CF-BDD scheme has promising advantages on various electrochemical and wearable sensor applications.