• Molecules and Cells
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• v.37 no.2
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• pp.100-108
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• 2014

Salmonella enterica serovar Typhimurium (S. Typhimurium) is an intracellular pathogen that has evolved to survive in the phagosome of macrophages. The periplasmic copper-binding protein CueP was initially known to confer copper resistance to S. Typhimurium. Crystal structure and biochemical studies on CueP revealed a putative copper binding site surrounded by the conserved cysteine and histidine residues. A recent study reported that CueP supplies copper ions to periplasmic Cu,Zn-superoxide dismutase (SodCII) at a low copper concentration and thus enables the sustained SodCII activity in the periplasm. In this study, we investigated the role of CueP in copper resistance at a high copper concentration. We observed that the survival of a cueP-deleted strain of Salmonella in macrophage phagosome was significantly reduced. Subsequent biochemical experiments revealed that CueP specifically mediates the reduction of copper ion using electrons released during the formation of the disulfide bond. We observed that the copper ion-mediated Fenton reaction in the presence of hydrogen peroxide was blocked by CueP. This study provides insight into how CueP confers copper resistance to S. Typhimurium in copper-rich environments such as the phagosome of macrophages.

• Journal of the Korean Society of Physical Medicine
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• v.8 no.2
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• pp.281-287
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• 2013

PURPOSE: The study was designed to investigate the effects of visual, auditory, and visuoauditory cues on simple Serial Task Performance in heaithy adults. METHODS: Sixty-three right-handed heaithy adults without history of neurological dysfunction were participated. A modified version of the Serial Reaction Time Task (SRTT) using five blocks of perceptual motor sequences was administered. The blocked paradigm consisted of the five blocks with randomly repeated 8 digit sequences with 5 repetition. Three types of sensory cue were employed: visual cue, auditory cue and visuoauditory cue. All subjects were assigned to press the matched botton as quickly and accurately as possible, when one of 8 stimulations was presented(one, two, three, four, five, six, seven, eight). The reaction time, accuracy, and P300 latency were measured during serial task performance. The mean reaction time(ms), accuracy(%), and P300 latency(ms) were compared between three types of cue using ANOVA. RESULTS: The reaction time to auditory cue was significantly longer than visual and visuoauditory cues(p<.001). And accuracy to auditory cue was significantly lower than visual and visuoauditory cues(p<.001). All P300 latency(at Fz, Cz, Pz) were significantly longer than to visual and visuoauditory cues(p<.05). CONCLUSION: It is suggested that type of cues influence in choice reaction. These data may helpful in designing not only effective motor learning training programs for healthy persons but also reeducation programs for patients with neurological dysfunction.

• Physical Therapy Korea
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• v.21 no.1
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• pp.20-28
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• 2014

The purpose of this study was to investigate the effects of visual cue deprivation during sideways treadmill training in individuals with stroke. Twenty-eight stroke patients were divided into two groups, and each group participated in a sideways treadmill training session for 20 minutes, three times per week for 4 weeks. The eyes close group (15 subjects) performed this treadmill training with visual cue deprivation, while the eyes open group (13 subjects) performed it without visual cue deprivation. Gait function was measured in both groups before and after the training sessions with the Biodex Gait Trainer 2, which determined walking speed, distance, step length, and time on each foot. Balance was measured before and after each training period in both groups using the Five-Times Sit-to-Stand Test (FTSST), the Timed Up and Go test (TUG), and the seven-item Berg Balance Scale-3P (7-item BBS-3P). The eyes close group showed significantly improved gait function, walking speed, distance, step length, and time on each foot after training (p<.05). The eyes close group showed improved balance ability, FTSST, TUG, and 7-item BBS-3P test after training (p<.05). The findings indicated that sideways treadmill training with visual cue deprivation positively affects gait ability of stroke patients. Therefore, sideways treadmill training with visual cue deprivation may be useful for the recovery of gait ability of stroke patients.

• The Journal of Korean Physical Therapy
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• v.21 no.4
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• pp.51-56
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• 2009

Purpose: This study examined the difference in the static balance ability according to the visual cues and postural tasks in normal subjects. Methods: Thirty participants (12 male, 18 female; mean age $24.63\pm1.43$ years) stood barefoot on a force platform in a one-legged stance, tandem Romberg stance and tandem Romberg with neck extension stance with a visual cue open and closes. The static balance was assessed by the center of pressure (CoP), surface electromyography root mean square (RMS) of the leg muscles according to the stance position. Results: In the CoP tests, the difference in the unit path length and circumference area was affected by the visual cue according to the stance posture (p<0.01). In the RMS tests, the difference in the tibialis anterior and medial gastrocnemius muscle was affected by visual cue in accordance with the stance posture (p<0.01). Conclusion: The visual cue and postural task affect the balance ability in normal subjects. Therefore, this study provides clinical evidence that the balance and postural control can be improved. Therapeutic intervention, such as an obstacle course, and a lower leg muscle performance program with a change in the base of support can affect the balance and postural control.

• Clinical and Experimental Reproductive Medicine
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• v.26 no.1
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• pp.83-87
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• 1999

Since the blastocyst is broken and spreads out on a flat plastic culture dish (two dimensional culture) during in vitro development, it has been difficult to study the implantation process. It also has been difficult to analyse the interactions between endometrial epithelial and stromal cells because of the lack of a long-term in vitro model which can stimulate in vivo characteristics, as these cells eventually fail to proliferate or cease to express differentiated functions. Recently nontransformed cell lines, CUE-P and CUS-V2, derived from rat endometrial epithelium and stroma were reported. In this study, morphology of CUE-P and CUS-V2 was examined and oxytocin gene expression by CUE-P cells was demonstrated by RT-PCR. The CUE-P cells have a cuboidal morphology and CUS-V2 cells resemble fibroblast and exhibit a spindle-like morphology. In RT-PCR, same size of PCR products of oxytocin gene at hypothalamus, uterus and CUE-P cells were demonstrated. These results showed three dimensional culture system could be made by using the new cell lines.

• Journal of the Korean Academy of Clinical Electrophysiology
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• v.3 no.1
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• pp.71-84
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• 2005

This study had performed with purposes to analyze the influence of the change of vestibular sens, visual and proprioceptive sense to the postural sway, so as to supply the necessary clinical materials through developing the physical therapeutic interventions and assessment format for the diabetic neuropathy patients. The sample consisted of fifteen diabetic neuropathy patients with sensory disorder in their lower limbs and fifteen age-matched normal control group. Then the effect of the GVS and the visual cue open and closed to the postural sway were measured by CoP. The summary of the comparison results were obtained below. In the comparison of diabetes neuropathy patients group and age matched normal control group, however diabetes neuropathy patients group had a decrease in superficial tactile sense(p<.001) and nerve conduction velocity(p<.001), they were able to control the posture and walk. So it is, diabetes neuropaty patients had more disturbance compared with AMC group on at a hard surface, particularly in the visual cue open(p<.001) and visual cue closed(p<.01). Moreover, since diabetes neuropathy patients group had more differences in visual cue open and closed(p<. 01), GVS(p<.01), it meant that they're affected largely by vestibular sense, visual sense. In addition, since there're the largest change in doubled sense disturbance such as visual cue open and closed under GVS, it meant that compensation of other senses were quite important for the diabetes neuropathy patients' postural control. In the conclusion, diabetes neuropathy patients who decrease or lose the somatosensory system, sensory training of visual and vestibular system are likely to be quite essential to control the posture and balance.

• Molecules and Cells
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• v.38 no.8
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• pp.715-722
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• 2015

In Gram-negative bacteria in the periplasmic space, the dimeric thioredoxin-fold protein DsbC isomerizes and reduces incorrect disulfide bonds of unfolded proteins, while the monomeric thioredoxin-fold protein DsbA introduces disulfide bonds in folding proteins. In the Gram-negative bacteria Salmonella enterica serovar Typhimurium, the reduced form of CueP scavenges the production of hydroxyl radicals in the copper-mediated Fenton reaction, and DsbC is responsible for keeping CueP in the reduced, active form. Some DsbA proteins fulfill the functions of DsbCs, which are not present in Gram-positive bacteria. In this study, we identified a DsbA homologous protein (CdDsbA) in the Corynebacterium diphtheriae genome and determined its crystal structure in the reduced condition at $1.5{\AA}$ resolution. CdDsbA consists of a monomeric thioredoxin-like fold with an inserted helical domain and unique N-terminal extended region. We confirmed that CdDsbA has disulfide bond somerase/reductase activity, and we present evidence that the N-terminal extended region is not required for this activity and folding of the core DsbA-like domain. Furthermore, we found that CdDsbA could reduce CueP from C. diphtheriae.

• Journal of the Korean Society of Physical Medicine
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• v.7 no.4
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• pp.411-421
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• 2012

PURPOSE: The purpose of this study was to investigate the effect of balance rehabilitation training with the visual cue deprivation on gait function in stroke patients in comparison with balance training without the visual cue deprivation. METHODS: Twenty two stroke patients participated in this study. Patients were randomly assigned to one of the two balance training program with and without the visual cue deprivation. Balance training session for each group lasted 50 minutes, 3 times a week for a total of 6 weeks. Gait function was measured with the Functional Gait Assessment (FGA), the self-selective comfortable gait speed (CGS), the maximal gait speed (MGS), and the Gait Analysis System. Temporal and spatial gait parameters of each evaluation were measured before and after the balance training program respectively. RESULTS: After the program, the visual cue deprivation group improved significantly in the FGA, the CGS, the gait velocity, the step time, the step length, the stride length, and the Functional Ambulation Performance (FAP) in comparison with the balance training group with the visual cue (p<.05). CONCLUSION: The gait function of the participants with the visual cue deprivation showed more improvement after the balance training program compared to the patients group without the visual cue deprivation, Therefore, the balance training program with the visual cue deprivation may be useful for rehabilitation of patients with chronic stroke.

• The Journal of Korean Physical Therapy
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• v.34 no.5
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• pp.212-217
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• 2022

Purpose: This study sought to investigate the effects of visual cue deprivation balance training by applying head control feedback to the balance function and the fall index in older people. Methods: The study was conducted on 26 older people at the S Hospital in Gyeongsansi. The patients were randomly divided into the experimental groupI (EGI, n=9), the experimental groupII (EGII, n=9), and the control group (CG, n=8). The three groups were trained for 30 minutes three times a week for four weeks. To assess the patients' static balance function, their limits of stability (LOS) was measured using the BioRescue system, (RM Ingenierie, France), composed of a pressure platform that can measure force in diverse ways, a computer, and a monitor. The dynamic balance function was measured using the Berg Balance Scale (BBS). Also, the fall index was measured to evaluate the risk of falling. A paired t-test was performed to compare pre- and post-training performance within the groups. One-way ANOVA was performed for comparing the three groups. A post hoc least significant difference (LSD) test was also performed. Results: The results of the LOS showed a significant difference after training in the EGI and EGII groups (p<0.05). As a result of the difference between the three groups, there was a significant difference after training (p<0.05). The results of the BBS showed a significant difference after training in the EGI group (p<0.05). As a result of the difference between the three groups, there was a significant difference after training (p<0.05). Conclusion: Visual cue deprivation balance training applying head control feedback is effective in improving the dynamic balance function in older people. It is also necessary to constantly maintain the head orientation by feedback and to properly control the head movement.

• Science of Emotion and Sensibility
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• v.21 no.2
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• pp.29-42
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• 2018

As information technology is more actively incorporated into automobiles, the role of IVIS (In-Vehicle Infotainment System) is becoming increasingly important for providing convenience and entertainment for drivers. However, using the infotainment systems while driving requires task switching and attending to two visual resources simultaneously. We simulated a setting where participants have to drive while interacting with the infotainment system and examined how task difficulty and motor cues impact driver task-switching and driving performance, specifically whether the effects of motor cues differ depending on task difficulty. For the infotainment display, we used two types of number array depending on the congruency between the digit repetition and the chunking unit, while task difficulty was manipulated by the size of the touch-keys. Participants were instructed to dial two numbers on the screen while we recorded the dialing time, lateral position, inter-key press intervals, and steering wheel control. We found that dialing time and lateral position were affected by task difficulty, while the type of number array had no effect. However, the inter-key press intervals between chunked numbers and steering wheel movement both increased when participants had to use an incongruent number array, which indicates that, if number digits are repeated, chunking is ignored by the drivers. Our findings indicate that, in a dual-task condition, motor cues offset the effect of chunking and can effectively signal the timing for task switching.