• Proceedings of the KSME Conference
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• 2004.11a
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• pp.910-915
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• 2004

The objective of this study is to investigate the influence of process parameters, such as power of laser, travel speed of laser and material thickness, on the practical cutting region and the kerfwidth for the case of cutting of CSP 1N sheet using high power Nd:YAG laser with continuous wave(CW). In order to find the practical cutting region and the relationship between process parameters on the kerfwidth, several laser cutting experiments are carried out. The effective heat input is introduced to consider the influence of power and travel speed of laser on the kerfwidth together. From the results of experiments, the allowable cutting region and the relationship between the effective heat input and kerfwidth for the case of cutting of CSP 1N sheet using high power CW Nd:YAG laser have been obtained to improve the dimensional accuracy of the cut area.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.5 no.1
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• pp.51-58
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• 2006

The objective of this research work is to investigate into heat transfer characteristics of the laser cutting of CSP 1N sheet using high power CW Nd:YAG laser. In order to investigate the heat transfer characteristics, three dimensional quasi stationary and steady-state heat transfer analysis has been carried out. The laser heat source is assumed as a volumetric heat source with a gaussian heat distribution in a plane. Through the comparison of the results of analyses with those of experiments, the proper finite element model has been obtained. In addition, characteristics of the three-dimensional heat transfer and temperature distribution have been estimated by the finite element model. Finally, the minimum temperature at the center for cutting of the material has been estimated.

• Journal of the Korean Society for Precision Engineering
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• v.22 no.7 s.172
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• pp.19-26
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• 2005

The objective of this study is to investigate the influence of process parameters, such as power of laser, cutting speed of laser and material thickness, on the practical cutting region and the kerfwidth fer the case of cutting of CSP IN sheet using high power Nd:YAC laser in continuous wave(CW) mode. In order to obtain the practical cutting region and the relationship between process parameters on the kerfwidth, several laser cutting experiments are carried out. The effective heat input is introduced to consider the influence of power and cutting speed of laser on the kerfwidth together. From the results of experiments, the allowable cutting region and the relationship between the effective heat input and kerfwidth fur the case of cutting of CSP 1N sheet using high power CW Nd:YAG laser have been obtained to improve the dimensionalaccuracyofthecutarea.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2005.06a
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• pp.418-421
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• 2005

The objective of this research work is to investigate the effects of process parameters, such as power of laser, travel speed of laser and material thickness, on kerfwidth and characteristics of the cut surface for the case of cutting of CSP 1N sheet using high power continuous wave Nd:YAG laser. In order to find relationship between the process parameters on the quality of the cut section, such as kerfwidth, surface roughness and the striation formation, several laser cutting experiments are carried out. From the results of experiments, an optimal cutting speed for each cutting condition has been obtained to improve the quality of the cut surface.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2005.10a
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• pp.162-165
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• 2005

The objective of this research work is to investigate into the three-dimensional temperature distribution using quasi steady-state heat transfer analysis fur the case of the laser cutting of CSP 1N sheet using high power CW Nd:YAG laser. The laser heat source is assumed as a volumetric heat source with a gaussian heat distribution in a plane. Through the comparison of the results of analyses with those of the experiments, the optimal finite element model is obtained. Finally, characteristics of the three-dimensional heat transfer and temperature distribution have been estimated by the optimal finite element model.

• Proceedings of the Korean Society of Machine Tool Engineers Conference
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• 2004.10a
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• pp.286-291
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• 2004

The objective of this research work is to investigate the influence of process parameters, such as power of laser, travel speed of laser and material thickness, on roughness and striation of the cut surface for the case of cutting of CSP 1N sheet using high power Nd:YAG laser with continuous wave(CW). In order to find the practical cutting region and the relationship between process parameters on the roughness and the striation, several laser cutting experiments are carried out. From the results of experiments, the allowable cutting region and an optimal cutting speed for each cutting condition have been obtained to improve the quality of the cut surface. In addition, it has been shown that the surface roughness is related to the number of striation and depth of valley of the cut surface.

• Transactions of the Korean Society of Automotive Engineers
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• v.2 no.1
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• pp.73-83
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• 1994

Press formabilities of aluminum sheets for automobile body were investigated. Plane strain stretching test (called RIST-PSST), cupping test and U bending test were performed to assess the press formability of aluminum sheets respectively. The results showed that aluminum sheets are generally inferior to cold-rolled steel sheet of deep drawing quality (CSP3N) in press formability. The limiting punch height (LPH) and limiting plane strain (FLCo) of aluminum sheets are 50%-70% level compared to that of CSP3N. Moreover, the limiting drawing ratios(LDR) of aluminum sheets are ranged between 1.95 and 2.1. The poor press formability of aluminum sheets is responsible for low values of total elongation and plastic anisotropy parameter in tensile characteristic. The shape fixability of aluminum sheets evaluated in U bending test is very poor due to its low elastic modulus compared to CSP3N.

• Journal of the Korean Society for Precision Engineering
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• v.23 no.7 s.184
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• pp.30-38
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• 2006

Laser cutting technology is one of flexible rapid manufacturing technologies with various advantageous including a high cutting speed, manufacturing of parts with a complex shape and others. The quality of the cut part and the optimum cutting conditions are highly dependent on the combination of the process parameters. The objective of this research works is to investigate the influence of process parameters, such as power of laser, cutting speed of laser and material thickness, on the surface roughness and the striation formation of the cut section for the case of cutting of CSP 1N sheet using high power Nd:YAG Laser with a continuous wave (CW). In order to find the relationship between process parameters and the surface roughness and the striation formation of the cut section, several experiments are carried out. Through the investigation of the empirical results, it has been shown that the surface roughness is highly related to the striation formation, including the frequency and angle of the striation, of the cut section. From the results of experiments, an optimum cutting speed for each cutting condition has been obtained to improve both the quality of the cut surface and the cutting efficiency.

• Bulletin of the Korean Chemical Society
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• v.33 no.8
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• pp.2508-2512
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• 2012

Cold shock proteins (CSPs) are a family of proteins induced at low temperatures. CSPs bind to single-stranded nucleic acids through the ribonucleoprotein 1 and 2 (RNP 1 and 2) binding motifs. CSPs play an essential role in cold adaptation by regulating transcription and translation via molecular chaperones. The solution nuclear magnetic resonance (NMR) or X-ray crystal structures of several CSPs from various microorganisms have been determined, but structural characteristics of psychrophilic CSPs have not been studied. Therefore, we optimized the purification process to obtain highly pure Lm-Csp and determined the three-dimensional structure model of Lm-Csp by comparative homology modeling using MODELLER on the basis of the solution NMR structure of Bs-CspB. Lm-Csp consists of a ${\beta}$-barrel structure, which includes antiparallel ${\beta}$ strands (G4-N10, F15-I18, V26-H29, A46-D50, and P58-Q64). The template protein, Bs-CspB, shares a similar ${\beta}$ sheet structure and an identical chain fold to Lm-Csp. However, the sheets in Lm-Csp were much shorter than those of Bs-CspB. The Lm-Csp side chains, E2 and R20 form a salt bridge, thus, stabilizing the Lm-Csp structure. To evaluate the contribution of this ionic interaction as well as that of the hydrophobic patch on protein stability, we investigated the secondary structures of wild type and mutant protein (W8, F15, and R20) of Lm-Csp using circular dichroism (CD) spectroscopy. The results showed that solvent-exposed aromatic side chains as well as residues participating in ionic interactions are very important for structural stability. Further studies on the three-dimensional structure and dynamics of Lm-Csp using NMR spectroscopy are required.