• Asian Pacific Journal of Cancer Prevention
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• 제15권20호
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• pp.8679-8684
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• 2014

CD133 was recently reported to be a cancer stem cell and prognostic marker. Quercetin is considered as a potential chemopreventive agent due to its involvement in suppression of oxidative stress, proliferation and metastasis. In this study, the expression of CD133/CD44 in esophageal carcinomas and Eca109/9706 cells was explored. In immunoflurorescence the locations of $CD133^+$ and multidrug resistance 1 $(MDR1)^+$ in the same E-cancer cells were coincident, mainly in cytomembranes. In esophageal squamous cell carcinomas detected by double/single immunocytochemistry, small $CD133^+$ cells were located in the basal layer of stratified squamous epithelium, determined as CSLC (cancer stem like cells); $CD44^+$ surrounding the cells appeared in diffuse pattern, and the larger $CD44^+$ (hi) cells were mainly located in the prickle cell layer of the epithelium, as progenitor cells. In E-cancer cells exposed to nanoliposomal quercetin (nLQ with cytomembrane permeability), down-regulation of NF-${\kappa}Bp65$, histone deacetylase 1 (HDAC1) and cyclin D1 and up-regulation of caspase-3 were shown by immunoblotting, and attenuated HDAC1 with nuclear translocation and promoted E-cadherin expression were demonstrated by immunocytochemistry. In particular, enhanced E-cadherin expression reflected the reversed epithelial mesenchymal transition (EMT) capacity of nLQ, acting as cancer attenuator/preventive agent. nLQ acting as an HDAC inhibitor induced apoptotic cells detected by TUNEL assay mediated via HDAC-NF-${\kappa}B$ signaling. Apoptotic effects of liposomal quercetin (LQ, with cytomembrane-philia) combined with CD133 antiserum were also detected by CD133 immunocytochemistry combined with TUNEL assay. The combination could induce greater apoptotic effects than nLQ induced alone, suggesting a novel anti-CSC treatment strategy.

• 자원ㆍ환경경제연구
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• 제20권4호
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• pp.761-796
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• 2011

본 연구는 발전부문에서 에너지 정책의 목표인 에너지 안보와 기후변화 대책 간의 상호보완성을 고려하면서 최저 비용을 기준으로 도출된 발전 구성비를 나타내었다. 에너지 안보의 수준을 측정하기 위해 에너지 안보 가격지수를 도입하여 화석연료의 공급 집중도에서 비롯된 안보 위협을 평가하였다. CSC 방법론의 적용을 통해 원자력과 육상풍력이 석탄가스화 복합발전 및 미분탄 화력발전을 대체하는 경우가 비용효율적인 대체안으로 나타났으며, 잠재적인 탄소 감축 가능량 및 안보 개선폭을 확인하였다. 또한 이러한 대체안이 탄소 감축뿐만 아니라 에너지 안보의 개선을 달성하는 상호보완성을 확인하였다. 추가적인 에너지 안보의 개선을 위해서는 화석연료의 해외자원개발을 통한 에너지 자급률을 제고하는 것이 최선의 대안으로 나타났으며, 이를 기반으로 2020년의 특정 목표치를 달성하기 위해 최저 비용을 나타내는 발전구성비를 도출하였다.

• Molecules and Cells
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• 제44권7호
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• pp.481-492
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• 2021

Tribbles homolog 2 (TRIB2) is implicated in tumorigenesis and drug resistance in various types of cancers. However, the role of TRIB2 in the regulation of tumorigenesis and drug resistance of cancer stem cells (CSCs) is still elusive. In the present study, we showed increased expression of TRIB2 in spheroid-forming and aldehyde dehydrogenase-positive CSC populations of A2780 epithelial ovarian cancer cells. Short hairpin RNA-mediated silencing of TRIB2 expression attenuates the spheroid-forming, migratory, tumorigenic, and drug-resistant properties of A2780 cells, whereas overexpression of TRIB2 increases the CSC-like characteristics. TRIB2 overexpression induced GSK3β inactivation by augmenting AKT-dependent phosphorylation of GSK3β at Ser9, followed by increasing β-catenin level via reducing the GSK3β-mediated phosphorylation of β-catenin. Treatment of TRIB2-ovexpressed A2780 cells with the phosphoinositide3-kinase inhibitor LY294002 abrogated TRIB2-stimulated proliferation, migration, drug resistance of A2780 cells. These results suggest a critical role for TRIB2 in the regulation of CSC-like properties by increasing the stability of β-catenin protein via the AKT-GSK3β-dependent pathways.

• 산업경영시스템학회지
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• 제42권3호
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• pp.176-183
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• 2019

The environmental changes in the Korean cosmetic medical service industry in the $21^{st}$ century are forming intense competition among medical institutions due to the quantitative expansion of its market. For stable growth of the cosmetic medical service industry, continuous quality improvement is necessary based on empirical research on the quality of cosmetic medical services rather than external expansion. The purpose of this study is to classify the quality attributes of cosmetic medical service using Kano model and to derive the degree of satisfaction and dissatisfaction of each quality attributes through Customer Satisfaction Coefficient (CSC). Through this, the study identified strategic priorities and suggested specific step-by-step approaches and quality improvement priorities that can increase customer satisfaction using the Potential Customer Satisfaction Improvement Index (PCSI Index). Based on SERVPERF, this study used measurement tools consisting of five dimensions : tangibles, reliability, responsiveness, assurance, and empathy. In addition, it was used of measurement items reconstructed into positive, negative, and satisfaction questions for Kano model analysis, CSC analysis, and PCSI Index analysis. A total of 300 medical consumers who experienced cosmetic medical services for the past one year in medical institutions such as plastic surgery and dermatology were collected with convenient sampling. As a result, urgent items for improving the quality of service using the PCSI Index, 'Consideration for customer benefits' in empathy category was followed by 'Immediate help' and 'Sincere response' in responsiveness category, and 'Understanding customer needs' in empathy category, respectively. That is, it is required to improve human service quality attributes such as empathy and responsiveness rather than physical service quality attributes. This study contributes practically in that it provides specific and discriminatory approaches to improve customer satisfaction on cosmetic medical service quality and suggests improvement priorities.

• Nutrition Research and Practice
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• 제16권2호
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• pp.161-172
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• 2022

BACKGROUND/OBJECTIVES: Colorectal cancer (CRC) is the third most common cancer worldwide and has a high recurrence rate, which is associated with cancer stem cells (CSCs). β-carotene (BC) possesses antioxidant activity and several anticancer mechanisms. However, no investigation has examined its effect on colon cancer stemness. MATERIALS/METHODS: CD133⁺CD44⁺ HCT116 and CD133⁺CD44⁺ HT-29 cells were isolated and analyzed their self-renewal capacity by clonogenic and sphere formation assays. Expressions of several CSCs markers and Wnt/β-catenin signaling were examined. In addition, CD133⁺CD44⁺ HCT116 cells were subcutaneously injected in xenograft mice and analyzed the effect of BC on tumor formation, tumor volume, and CSCs markers in tumors. RESULTS: BC inhibited self-renewal capacity and CSC markers, including CD44, CD133, ALDH1A1, NOTCH1, Sox2, and β-catenin in vitro. The effects of BC on CSC markers were confirmed in primary cells isolated from human CRC tumors. BC supplementation decreased the number and size of tumors and delayed the tumor-onset time in xenograft mice injected with CD133⁺CD44⁺ HCT116 cells. The inhibitory effect of BC on CSC markers and the Wnt/β-catenin signaling pathway in tumors was confirmed in vivo as well. CONCLUSIONS: These results suggest that BC may be a potential therapeutic agent for colon cancer by targeting colon CSCs.

• 대한토목학회논문집
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• 제42권2호
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• pp.239-248
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• 2022

최근 건설환경의 변화에 따른 건설프로젝트에서 리스크는 현저하게 증가하고 있다. 리스크를 기회로 인식하는 적극적인 리스크관리가 요구된다. 본 연구의 목적은 Kano 모델, Timko CSC (Customer Satisfaction Coefficient), ASC (Average Satisfaction Coefficient)를 활용한 비교 분석을 통해 중요도 결정 방법의 리스크관리 모델을 제안하는 것이다. 선행연구를 바탕으로 델파이기법을 활용하여 실무자 면담을 통해 Kano 모델을 수정한 설문지를 통해 리스크관리 요인 결정의 타당성을 검토한다. 이를 통해 국내 건설프로젝트 실무자가 인식하는 핵심 리스크관리 요인을 선정하여 적합한 리스크관리 모델을 제시한다. 연구 결과, 건설프로젝트 리스크관리 검증을 위해 개발된 Kano 모델은 실무자의 리스크관리를 검증하는데 유효한 것으로 평가되었다. 본 연구에서 제시한 Kano 모델이 건설프로젝트 리스크관리의 중요도를 검증하는 데에 적극적으로 활용되기를 기대한다.

• 통상정보연구
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• 제18권4호
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• pp.55-78
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• 2016

uTradeHub 관련 선행연구들은 서비스 품질의 중요성을 일원론적 시각에서 파악했을 뿐 어떠한 서비스 품질속성을 우선적으로 향상시켜야 할지에 대한 연구는 부재한 상황이다. 본 연구에서는 Kano 모형에 의거 uTradeHub의 서비스 품질속성을 분류하고, Timko의 고객만족계수(CSC)와 평균만족계수(ASC)를 사용하여 uTradeHub 서비스 품질 제고를 위해 중점적으로 관리해야 하는 품질속성을 규명하였다. 설문조사를 통해 확보된 총105개의 유효자료를 실증분석에 사용하였으며, 연구결과를 요약하면 다음과 같다. 첫째, Kano모형에 의거 서비스 품질속성을 분류한 결과, 일원적 품질 12개, 당연적 품질 5개, 무관심 품질 2개가 도출되었다. 둘째, Timko의 고객만족계수(CSC)를 이용한 분석결과에서는 "고충 및 불만에 대한 사후처리"와 "비용절감", "업무처리의 효율성", "정확하고 유용한 정보 제공"이 만족계수(SC)의 상위에 랭크됨으로써 서비스 품질이 충족되면 고객만족이 특히 증가하는 품질속성으로 파악되었다. 반편 "고충 및 불만에 대한 사후관리", "상호작용", "문제발생시 즉각적 대응능력", "정확하고 유용한 정보제공", "업무처리의 효율성" 등은 불만족계수(DC)의 상위에 랭크됨으로써 서비스 품질이 충족되지 않으면 고객 불만이 특히 증가하는 품질속성으로 분석되었다. 셋째, 평균만족계수(ASC)에 의거 uTradeHub 서비스 품질 제고를 위해 중점 관리해야 하는 품질속성을 분석한 결과에서는 "고충 및 불만에 대한 사후관리", "비용절감", "정확하고 유용한 정보 제공", "업무처리의 효율성", "업무성과" 등이 상위 5위에 랭크됨으로써 지금보다 개선되면 만족도가 더 크게 증가하지만 악화되면 만족도가 훨씬 더 감소하는 품질속성으로 분류되었다.

• 한국식품영양과학회지
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• 제31권5호
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• pp.770-774
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• 2002

레토르트 굴죽 개발을 위하여 원료의 조성 및 가공방법이 죽의 물성 및 기호도에 미치는 영향에 대하여 연구하였다. 쌀의 투입량은 10～12% 정도로 유지할 때 죽의 점도가 약 800 cp를 유지하여 적당했는데 쌀알의 안정성을 높이고 안정된 질감을 확보하기 위하여 사용한 쌀의 1/2을 마쇄하여 사용하는 것이 점도 향상 및 기호도에 유리하였다. 전분 원료 중 감자전분, 찰옥수수전분 및 Perfectamyl AC전분은 호화온도에 상관없이 거의 일정한 점도를 유지하여 작업시 온도 편차에 따른 품질 변화를 유발하지 않기 때문에 좋은 조건을 지니고 있어 레토펀트 죽의 제조에 좋은 조건을 지니고 있었으며 특히 찰옥수수전분은 살균 후 소비시에는 오히려 점도가 감소하여 일정한 점도를 유지하는 특징이 있어 레토르트 죽에 적합할 것으로 판단되었다. 변성 전분 중 Purity CSC전분은 냉-해동 후 조직 이 호화온도에 따라 묽은 풀과 같은 정도에서 순두부 정도로 나타났으며 호화 온도에 무관하게 수분분리가 적으므로 찰옥수수전분에 약 25% 정도를 혼합하여 사용할 때 좋은 물성을 유지할 수 있었다. 레토르트 굴죽의 원료 분산성을 개선하기 위하여 Kan-than gum을0.2%정도 사용하면 식감에 영향을 미치지 않고 품질 안정성을 유지할 수 있었다. 따라서 레토르트 굴죽의 최적 조성을 쌀 10%(이중 1/2은 마쇄), 찰옥수수전분 1.5%, Purity CSC 0.3%, xanthan gm, 0.2%, 식염 0.3%, 물 87.5%로 완성하였고 최적 조건 하에서 완성된 레토르트 굴죽을 상온에서 5개월 동안 저장한 후에도 안정된 물성이 확인되어 이들 원료의 가공적성이 레토르트 굴죽에 적합함을 확인하였다.

• Journal of Biosystems Engineering
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• 제43권1호
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• pp.21-29
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• 2018

Purpose: The objective of this study is to evaluate the performance of the conical solar concentrator (CSC) system, whose design is focused on increasing its collecting efficiency by determining the optimal conical angle through a theoretical study. Methods: The design and thermal performance analysis of a solar concentrator system based on a $45^{\circ}$ conical concentrator were conducted utilizing different mass flow rates. For an accurate comparison of these flow rates, three equivalent systems were tested under the same operating conditions, such as the incident direct solar radiation, and ambient and inlet temperatures. In order to minimize heat loss, the optimal double tube absorber length was selected by considering the law of reflection. A series of experiments utilizing water as operating fluid and two-axis solar tracking systems were performed under a clear or cloudless sky. Results: The analysis results of the CSC system according to varying mass flow rates showed that the collecting efficiency tended to increase as the flow rate increased. However, the collecting efficiency decreased as the flow rate increased beyond the optimal value. In order to optimize the collecting efficiency, the conical angle, which is a design factor of CSC, was selected to be $45^{\circ}$ because its use theoretically yielded a low heat loss. The collecting efficiency was observed to be lowest at 0.03 kg/s and highest at 0.06 kg/s. All efficiencies were reduced over time because of variations in ambient and inlet temperatures throughout the day. The maximum efficiency calculated at an optimum flow rate of 0.06 kg/s was 85%, which is higher than those of the other flow rates. Conclusions: It was reasonable to set the conical angle and mass flow rate to achieve the maximum CSC system efficiency in this study at $45^{\circ}$ and 0.06 kg/s, respectively.

• Asian-Australasian Journal of Animal Sciences
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• 제26권5호
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• pp.646-653
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• 2013

Four crossbred Sindhi heifers with an average body weight (BW) of 135 kg and a mean age of 17 months were used to investigate the effect of feeding different combinations of rice straw and ensiled water hyacinth (EWH) supplemented with a source of protein in the form of cottonseed cake (CSC) on intake and digestibility. Four treatments consisting of graded levels of EWH were arranged in a $4{\times}4$ Latin square. The levels of EWH were set at: 0 (EWH0), 15 (EWH15), 30 (EWH30), and 45% (EWH45) of an expected total dietary dry matter (DM) intake of 30 g total DM per kg BW per day. Rice straw was offered ad libitum, while CSC was given at a fixed level of 5 g DM/kg body weight (BW). Voluntary intake and digestibility were measured consecutively in the 4 experimental periods which each lasted 28 days. The crude protein (CP) content of EWH, rice straw and CSC were 174, 53 and 370 g/kg DM, respectively. Rice straw had the highest neutral detergent fibre (NDFom) content (666 g/kg DM), followed by EWH (503 g/kg DM) and the lowest content was 418 g/kg DM in the CSC. The actual EWH contents in the consumed diets were 0, 17, 32 and 52% for EWH0, EWH15, EWH30 and EWH45, respectively. Rice straw intake decreased with level of EWH offered from 3049 for EWH0 to 1014 g/day for EWH45. Crude protein intake was 16, 25 and 33% higher (p<0.001) in EWH15, EWH30 and EWH45 treatments, respectively, as compared to EWH0. Digestibility of organic matter (OM), CP, NDFom and acid detergent fibre (ADFom) increased with increasing level of EWH offered. The highest OM digestibility (72.2%) was found for treatment EWH45 and the lowest (47.4%) for treatment EWH0. In spite of similar dietary CP contents, CP digestibility increased by 21 (EWH15), 31 (EWH30) and 40% (EWH45) with increasing level of EWH in comparison with treatment EWH0. It is concluded that increasing level of EWH in cattle diets considerably improved CP intake and digestibility of nutrients.