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http://dx.doi.org/10.4162/nrp.2022.16.2.161

β-carotene regulates cancer stemness in colon cancer in vivo and in vitro  

Lee, Kyung Eun (Department of Nutritional Science and Food Management, Ewha Womans University)
Kwon, Minseo (Department of Nutritional Science and Food Management, Ewha Womans University)
Kim, Yoo Sun (Department of Nutritional Science and Food Management, Ewha Womans University)
Kim, Yerin (Department of Nutritional Science and Food Management, Ewha Womans University)
Chung, Min Gi (Department of Nutritional Science and Food Management, Ewha Womans University)
Heo, Seung Chul (Department of Surgery, Seoul National University-Seoul Metropolitan Government (SNU-SMG) Boramae Medical Center)
Kim, Yuri (Department of Nutritional Science and Food Management, Ewha Womans University)
Publication Information
Nutrition Research and Practice / v.16, no.2, 2022 , pp. 161-172 More about this Journal
Abstract
BACKGROUND/OBJECTIVES: Colorectal cancer (CRC) is the third most common cancer worldwide and has a high recurrence rate, which is associated with cancer stem cells (CSCs). β-carotene (BC) possesses antioxidant activity and several anticancer mechanisms. However, no investigation has examined its effect on colon cancer stemness. MATERIALS/METHODS: CD133+CD44+ HCT116 and CD133+CD44+ HT-29 cells were isolated and analyzed their self-renewal capacity by clonogenic and sphere formation assays. Expressions of several CSCs markers and Wnt/β-catenin signaling were examined. In addition, CD133+CD44+ HCT116 cells were subcutaneously injected in xenograft mice and analyzed the effect of BC on tumor formation, tumor volume, and CSCs markers in tumors. RESULTS: BC inhibited self-renewal capacity and CSC markers, including CD44, CD133, ALDH1A1, NOTCH1, Sox2, and β-catenin in vitro. The effects of BC on CSC markers were confirmed in primary cells isolated from human CRC tumors. BC supplementation decreased the number and size of tumors and delayed the tumor-onset time in xenograft mice injected with CD133+CD44+ HCT116 cells. The inhibitory effect of BC on CSC markers and the Wnt/β-catenin signaling pathway in tumors was confirmed in vivo as well. CONCLUSIONS: These results suggest that BC may be a potential therapeutic agent for colon cancer by targeting colon CSCs.
Keywords
Beta carotene; colorectal cancer; cancer stem cells;
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