• Journal of Life Science
• /
• v.28 no.11
• /
• pp.1277-1284
• /
• 2018

In this study, a repeated yeast integrative plasmid (R-YIp) harboring Cre/loxP system was constructed to integrate various gene expression cassettes into the yeast chromosome. The R-YIp system contains a reusable selective marker (CgTRP1), loxP sequence, and target sequence for integration. Therefore, many gene expression cassettes can be integrated into the same position of the same yeast chromosome. In the present study, several model enzymes involving xylan/xylose metabolism were examined, including endoxylanase (XYLP), ${\beta}$-xylosidase (XYLB), xylose reductase (GRE3) and xylitol dehydrogenase (XYL2). Efficient expression of these genes was obtained using two promoters (GAL10p and ADH1p) and various plasmids (pGMF-GENE and pAMF-GENE plasmids) were constructed. The XYLP, XYLB, GRE3, and XYL2 genes were efficiently expressed under the control of the GAL10 promoter. Subsequently, R-YIps containing the GAL10p-GENE-GAL7t cassette were constructed, resulting in pRS-XylP, pRS-XylB, pRS-Gre3, and pRS-Xyl2 plasmids. These plasmids were sequentially integrated into chromosome VII of a Saccharomyces cerevisiae strain by repeated gene integration and selective marker rescue. These genes were integrated by the R-YIp system and were stably expressed in the yeast transformants to produce active recombinant enzymes. Therefore, we expect that the R-YIp system will be able to overcome current limitations of the host cells and allow selective marker selection for the integration of various genes into the yeast chromosome.

• Journal of Korea Port Economic Association
• /
• v.37 no.4
• /
• pp.13-31
• /
• 2021

Since the signing of the Korea-Europe Free Trade Agreement, the volume of trade transactions between South Korea and Europe has increased. The traditional single-mode transport system has been transformed into an intermodal transport system using two or more modes of transport. In addition, the conventional sea and air transport routes have been restricted, leading to a decline in Korean exports to Europe, and the rail transport mode is becoming mainstream in the market due to the influence of COVID-19. This paper focuses on the China-Railway Express to explore a new intermodal transport route from Korea to Europe. First, the fuzzy analytic hierarchy process (AHP) is used to evaluate the factor weights when selecting intermodal transport routes from Korea to Europe. Then, the TOPSIS (Technique for Order of Preference by Similarity to Ideal Solution) method is used to rank three alternatives. The results show that among the four factors (total cost, total time, transportation capability, and service reliability), the total cost is the most significant factor, followed by the total time, service reliability, and transportation capability. Furthermore, the alternative route 1 (Incheon-Dalian-Manchuria-Hamburg) is preferred.

• Proceedings of the Korean Society of Computer Information Conference
• /
• 2016.07a
• /
• pp.101-102
• /
• 2016

본 논문에서는 차량의 특징점들 사이의 간격과 크기의 비례식으로 자동차의 차종을 식별하는 방법을 제시한다. 자동차 관련 영상은 그 편의성을 위하여 기본 RGB모델에서 Gray색상 모델로 변환시켜 사용한다. 자동차의 배경 제거는 Canny Edge Direction을 통하여 수행하고 외곽선 검을을 통하여 원하는 특징 점을 얻는다.

• Proceedings of the Korean Society of Computer Information Conference
• /
• 2017.01a
• /
• pp.47-48
• /
• 2017

본 논문에서는 Microsoft Azure 플랫폼의 Azure PowerShell, Azure CLI(Command Line Interface), REST API를 활용하여 클라우드 기반 서비스 포털과 관리 포털을 개발함으로서 중소건설사에서 건설현장의 공사원가 관리 및 일일 관리를 위한 모듈과 서비스 제공을 위해 필요한 서비스 포털 및 관리 포털과 제품 관리 모듈 등 클라우드 서비스 구축 수행하였다.

• Proceedings of the Korean Society of Computer Information Conference
• /
• 2017.01a
• /
• pp.203-204
• /
• 2017

SDLC 중에서 분석단계는 정보시스템의 개발을 준비하는 단계로서 정보기술 체계의 분석과 사용자의 요구 사항 도출 및 예측되는 위험 요소에 대한 평가도 함께 수행하였다. 정보보호의 요구사항은 도출은 기밀성, 무결성, 가용성, 그리고 책임 추적성 등의 관점에서 하였다.

• Proceedings of the Korean Society of Computer Information Conference
• /
• 2016.01a
• /
• pp.109-110
• /
• 2016

본 논문에서는 효율적으로 주차 공간을 확보와 주차장 성능을 향상을 위한 방법을 조사하였다. 이러한 방법으로는 차량 번호판 조사를 이용하여 주차 회전율을 구하는 방법이 있었다. 본 연구로 효율적으로 주차장을 사용하고 있는지를 판단할 수 있다. 또한 차량의 주차를 하여 잘 소통되는지를 알 수 있었다.

• Asian-Australasian Journal of Animal Sciences
• /
• v.21 no.5
• /
• pp.745-753
• /
• 2008

Gene-manipulated mice were discovered for the first time about a quarter century ago. Since then, numerous sophisticated technologies have been developed and applied to answer key questions about the fundamental roles of the genes of interest. Functional genomics can be characterized into gain-of-function and loss-of-function, which are called transgenic and knock-out studies, respectively. To make transgenic mice, the most widely used technique is the microinjection of transgene-containing vectors into the embryonic pronucleus. However, there are critical drawbacks: namely position effects, integration of unknown copies of a foreign gene, and instability of the foreign DNA within the host genome. To overcome these problems, the ROSA26 locus was used for the knock-in site of a transgene. Usage of this locus is discussed for the gain of function study as well as for several brilliant approaches such as conditional/inducible transgenic system, reproducible/inducible knockdown system, specific cell ablation by Cre-mediated expression of DTA, Cre-ERTM mice as a useful tool for temporal gene regulation, MORE mice as a germ line delete and site specific recombinase system. Techniques to make null mutant mice include complicated steps: vector design and construction, colony selection of embryonic stem (ES) cells, production of chimera mice, confirmation of germ line transmission, and so forth. It is tedious and labor intensive work and difficult to approach. Thus, it is not readily accessible by most researchers. In order to overcome such limitations, technical breakthroughs such as reporter knock-in and gene knock-out system, production of homozygous mutant ES cells from a single targeting vector, and production of mutant mice from tetraploid embryos are developed. With these upcoming progresses, it is important to consider how we could develop these systems further and expand to other animal models such as pigs and monkeys that have more physiological similarities to humans.

• Transactions of the Korean Society of Mechanical Engineers B
• /
• v.34 no.11
• /
• pp.965-973
• /
• 2010

Numerical analysis has been carried out to investigate the heat transfer characteristics of a plate heat exchanger. The multi-cell models with inlet part and outlet part are used for performing numerical simulation. The plate heat exchanger is characterized by chevron angle of $15^{\circ}$, corrugation pitch of 24mm and corrugation height 6~12mm. The length of the inlet-part considered in the analysis ranges from 24.8 to 124mm and Reynolds numbers range from 1,000 to 10,000. The correlations such as friction factor and Colburn factor are compared with previous experimental data. The results can be utilized for designing the plate heat exchanger.

• Journal of Microbiology and Biotechnology
• /
• v.22 no.8
• /
• pp.1133-1140
• /
• 2012

Ribonucleic acid interference (RNAi) inhibits the expression of target genes in a sequence-specific manner, and shows potential for gene knockdown in filamentous fungi, in which the locus-specific gene knockout occurs in low frequency. In this study, the function of the repressor of cellulase expression I (ACEI) was verified in Trichoderma koningii (T. koningii) YC01 through RNAi, and ace1-silenced strains with improved cellulase productivity were obtained. An expression cassette that transcribed the interfering double-stranded RNA (dsRNA) of ace1 was constructed and transformed into T. koningii, and the transformants, in which the expression of ace1 was successfully silenced, were selected. As a result of the ace1 gene silencing, the expression levels of the main cellulase and xylanase genes were elevated, and the enhanced production of total proteins, cellulase, and xylanase was observed in the cultivation. In addition, the down-regulation of ace1 resulted in an increasing expression of xyr1, but no clear variation in the expression of cre1, which suggested that ACEI acted as a repressor of the xyr1 transcription, but was not involved in the regulation of the cre1 expression. The results of this work indicate that ace1 is a valid target gene for enhancing enzyme production in T. koningii, and RNAi is an appropriate tool for improving the properties of industrial fungi.

• Journal of Korean Dental Science
• /
• v.9 no.1
• /
• pp.9-18
• /
• 2016

Purpose: Wnt signaling plays an essential role in the dental epithelium and mesenchyme during tooth morphogenesis. Deletion of the Wntless (Wls) gene in odontoblasts appears to reduce canonical Wnt activity, leading to inhibition of odontoblast maturation. However, it remains unclear if autonomous Wnt ligands are necessary for differentiation of dental pulp cells into odontoblast-like cells to induce reparative dentinogenesis, one of well-known feature of pulp repair to form tertiary dentin. Materials and Methods: To analyze the autonomous role of Wls for differentiation of dental pulp cells into odontoblast-like cells, we used primary dental pulp cells from unerupted molars of Wls-floxed allele mouse after infection with adenovirus for Cre recombinase expression to knockout the floxed Wls gene or control GFP expression. The differentiation of dental pulp cells into odontoblast-like cells was analyzed by quantitative real-time polymerase chain reaction. Result: Proliferation rate was significantly decreased in dental pulp cells with Cre expression for Wls knockout. The expression levels of Osterix (Osx), runt-related transcription factor 2 (Runx2), and nuclear factor I-C (Nfic) were all significantly decreased by 0.3-fold, 0.2-fold, and 0.3-fold respectively in dental pulp cells with Wls knockout. In addition, the expression levels of Bsp, Col1a1, Opn, and Alpl were significantly decreased by 0.7-fold, 0.3-fold, 0.8-fold, and 0.6-fold respectively in dental pulp cells with Wls knockout. Conclusion: Wnt ligands produced autonomously are necessary for proper proliferation and odontoblastic differentiation of mouse dental pulp cells toward further tertiary dentinogenesis.