• Bulletin of the Korean Chemical Society
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• v.20 no.9
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• pp.1073-1077
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• 1999

CRAMP, a 37-amino acid cationic antimicrobial peptide was recently deduced from the cDNA cloned from mouse femoral marrow RNA. In order to investigate the structure-activity relationship and functional region of CRAMP, CRAMP and its 18-mer overlapping peptides were synthesized by the solid phase method. CRAMP showed broad spectrum antibacterial activity against both Gram-positive and Gram-negative bacterial strains (MIC: 3.125-6.25 μM) but had no hemolytic activity until 50 μM. CRAMP was found to have a potent anticancer activity (IC50: 12-23 μM) against two human small cell lung cancer cell lines. Furthermore, CRAMP was found to display faster bactericidal rate in B. subtilis rather than E. coli in the kinetics of bacterial killing. Among 18-meric overlapping fragment peptides, only CRAMP (16-33) displayed potent antibacterial activity (MIC: 12.5-50 μM) against several bacteria with no hemolytic activity. Circular dichroism (CD) spectra anal-ysis indicated that CRAMP and its analogues will form the amphipathic α-helical conformation in the cell membranes similar to other antimicrobial peptides, such as cecropins and magainins.

• Bulletin of the Korean Chemical Society
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• v.24 no.10
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• pp.1478-1484
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• 2003

CRAMP was identified from a cDNA clone derived from a mouse femoral marrow cells as a member of cathelicidin-derived antimicrobial peptide. Tertiary structure of CRAMP in TFE/$H_2O$ (1 : 1, v/v) solution has been determined by NMR spectroscopy previously and consists of two amphipathic $\alpha-helices$ from Leu4 to Lys10 and from Gly16 to Leu33. These two helices are connected by a flexible region from Gly11 to Gly16. Analysis of series of fragments composed of various portion of CRAMP revealed that an 18-residue fragment with the sequence from Gly16 to Leu33 (CRAMP-18) was found to retain antibacterial activity without cytotoxicity. The effects of two Phe residues at positions 14 and 15 of CRAMP-18 on structure, antibacterial activity, and interaction with lipid membranes were investigated by $Phe^{14,15}$ ${\rightarrow}$ Ala substitution (CRAMP-18-A) in the present study. Substitution of Phe with Ala in CRAMP-18 caused a significant reduction on antibacterial and membrane-disrupting activities. Tertiary structures of CRAMP-18 in 50% TFE/$H_2O$ (1 : 1, v : v) solution shows amphipathic ${\alpha}$-helix, from $Glu^2{\;}to{\;}Leu^{18}$, while CRAMP-18-A has relatively short amphipathic ${\alpha}$-helix from $Leu^4{\;}to{\;}Ala^{15}$. These results suggest that the hydrophobic property of $Phe^{14}{\;}and{\;}Phe^15$ in CRAMP-18 is essential for its antibacterial activity, ${\alpha}$-helical structure, and interactions with phospholipid membranes.

• IMMUNE NETWORK
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• v.20 no.3
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• pp.25.1-25.13
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• 2020

Acinetobacter baumannii is known for its multidrug antibiotic resistance. New approaches to treating drug-resistant bacterial infections are urgently required. Cathelicidin-related antimicrobial peptide (CRAMP) is a murine antimicrobial peptide that exerts diverse immune functions, including both direct bacterial cell killing and immunomodulatory effects. In this study, we sought to identify the role of CRAMP in the host immune response to multidrug-resistant Acinetobacter baumannii. Wild-type (WT) and CRAMP knockout mice were infected intranasally with the bacteria. CRAMP^-/- mice exhibited increased bacterial colony-forming units (CFUs) in bronchoalveolar lavage (BAL) fluid after A. baumannii infection compared to WT mice. The loss of CRAMP expression resulted in a significant decrease in the recruitment of immune cells, primarily neutrophils. The levels of IL-6 and CXCL1 were lower, whereas the levels of IL-10 were significantly higher in the BAL fluid of CRAMP^-/- mice compared to WT mice 1 day after infection. In an in vitro assay using thioglycollate-induced peritoneal neutrophils, the ability of bacterial phagocytosis and killing was impaired in CRAMP^-/- neutrophils compared to the WT cells. CRAMP was also essential for the production of cytokines and chemokines in response to A. baumannii in neutrophils. In addition, the A. baumannii-induced inhibitor of κB-α degradation and phosphorylation of p38 MAPK were impaired in CRAMP^-/- neutrophils, whereas ERK and JNK phosphorylation was upregulated. Our results indicate that CRAMP plays an important role in the host defense against pulmonary infection with A. baumannii by promoting the antibacterial activity of neutrophils and regulating the innate immune responses.

• Journal of Microbiology and Biotechnology
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• v.16 no.9
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• pp.1429-1433
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• 2006

Application of recombinant protein production and particularly their isotopic enrichment has stimulated development of a range of novel multidimensional heteronuclear NMR techniques. Peptides in most cases are amenable to assignment and structure determination without the need for isotopic labeling. However, there are many cases where the availability of $^{15}N$ and/or $^{13}C$ labeled peptides is useful to study the structure of peptides with more than 30 residues and the interaction between peptides and membrane. CRAMP (Cathelicidin-Related AntiMicrobial Peptide) was identified from a cDNA clone derived from mouse femoral marrow cells as a member of cathelicidin-derived antimicrobial peptides. CRAMP was successfully expressed as a GST-fused form in E. coli and purified using affinity chromatography and reverse-phase chromatography. The yield of the CRAMP was 1.5 mg/l 1. According to CD spectra, CRAMP adopted ${\alpha}$-helical conformation in membrane-mimetic environments. Isotope labeling of CRAMP is expected to make it possible to study the structure and dynamic properties of CRAMP in various membrane systems.

• Journal of Korean Neurosurgical Society
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• v.46 no.1
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• pp.52-55
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• 2009

Writer's cramp is a type of idiopathic focal hand dystonia characterized by muscle cramps that accompany execution of the writing task specifically. There has been renewed interest in neurosurgical procedures for the treatment of dystonia over the past several years. In particular, deep brain stimulation (DBS) has received increasing attention as a therapeutic option for patients with dystonia. However, to date, limited reporters made investigations into DBS in relation to the Writer's cramp. In this case, unilateral Ventro-oralis complex (Vo) DBS resulted in a major improvement in patient's focal dystonic movement disorders. Her post-operative Burke-Fahn-Marsden Dystonia Rating (BFMDR) scale demonstrated 1 compared with pre-operative BFMDR scale 4. We conclude that thalamic Vo complex DBS maybe an important neurosurgical therapeutic option for Writer's cramp.

• Journal of Korean Medicine Rehabilitation
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• v.18 no.3
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• pp.211-217
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• 2008

Objectives : The purpose of this study is to evaluate the clinical effect of acupuncture and electroacupuncture by meridian theory to a patient with writer's cramp. Methods : We treated this patient with only acupuncture and electroacupuncture by meridian theory. We evaluated the state of improvement by time to maintain right 2nd finger extended, Pain Disability Index(PDI). PDI was measured twice before treatment and after 19 weeks. Results and Conclusions : The subject was improved in estimation by time to maintain right 2nd finger extended, PDI. The time was from a few seconds to almost 90 minutes. PDI was from 21 to 17. According to above result, acupuncture and electroacupuncture by meridian theory were effective to improve symptoms of writer's cramp.

• Proceedings of the KSMRM Conference
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• 2002.11a
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• pp.87-87
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• 2002

목적： Writer's cramp를 가진 환자와 정상 성인에서 뇌 활성화를 비교 하고자 한다. 대상 및 방법： Writer's cramp를 가진 1명의 환자와 정상 성인 4명에서 글을 쓰는 과제를 시행하여 관련된 대뇌영역의 활성화를 유도하였다. resting, writering을 반복하는 자극을 시행하였다. 자기공명영상은 3.0T 초전도 자기공명 영상장치에서 EPI BOLD기법을 이용하여 얻었다. 영상 후 처리는 SPM 분석 프로그램을 사용하였다.

• The Korean Journal of Pain
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• v.8 no.1
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• pp.117-119
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• 1995

Writer's cramp is a gradual onset and the disorder shows itself at first only when the patient is fatigued or stressed, or when a difficulty in controlling the pen leads to inaccurate writing. The prognosis is poor and treatment is often difficult and unsatisfactory. Case 1: A-30-year old male, who had a difficulty in writing with palpitation for 10 years in front of the other men. After 5 times of stellate ganglion block, palpitation during writing did not appear and after 15 times, he could write with a slight spasm of hand muscles. Case 2: A-40-year old male, who had a difficulty in writing in a stressful situation. After 28 times of stellate ganglion block, he could write in normal work except in the strong stressful situation. From our experience, we recommend the stellate ganglion block may be an effective treatment for a writer's cramp.

• Journal of Korean Physical Therapy Science
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• v.20 no.1
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• pp.17-25
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• 2013

The purpose of this research is to provide right information about deformation and to relieve fatigue of high-heels lovers. The research data includes 15 tests and survey on 71 female students. The result follows 1. Age of surveys is mostly 22, consisting 43.7% of all. The most frequently worn shoe kind is high heels that 45.1% of surveys wear 2. Female students those are 155~160cm high wear high heels most frequently, 40.8%. 3. The fatigue condition classified by hours of wearing: Surveys wearing high heels over 7 hours and 5~7 hours state starting to feel fatigue by 40.8%, 38.0% each, and the result was stastically significant 4. The appearance of pain on calf classified by hours of wearing: 35.2% of surveys answered they start to feel pain when worn high heels over 7 hours, and 33.8% of students answered 5~7 hours 5. The fatigue condition classified by kinds of shoes worn: 45.0% of the surveys felt tired when wearing high heels, 40.8% answered wearing heel inserted running shoes, and 14.0% for flat shoes. 6. The fatigue condition classified by heel height: 69.0% of survey answered they feel fatigue after wearing shoes with 5~9cm high heels, 21.1% answered under 3cm high heels, and 9.9% answered over 10cm heels(p<0.05) 7. The experience of cramp in calf cramp muscle classified by heel height: 69.0% of surveys experienced cramp when wearing 5~9cm high heels, 21.1% answered under 3cm high heels, 9.9% for over 10cm heels.

• Journal of the Korea institute for structural maintenance and inspection
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• v.12 no.2
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• pp.156-162
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• 2008

The fatigue durability test using the actual size beam was performed with a cramp joint in order to apply to the highway bridge deck slab. Three types of beam were investigated for durability performance by considering stress conditions in real bridge deck slabs, 1) A beam with major shear force applied at the joint (RC Type) 2) A beam with major bending moments applied at the joint (PSC Type) 3) A beam with the pure shear applied at the joint. The experiment for beams with cramp joints showed that the cramp joint had enough durability for fatigue regardless of the overlaid length of the looped distribution bars under the current design strength level. Moreover, it was clarified that the enough durability for fatigue under the load repetition was achieved by increasing the joint span grater than 1.5D with the consideration of the deformation due to reduction in joint stiffness.