• YAKHAK HOEJI
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• v.43 no.3
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• pp.385-390
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• 1999

Camptothecin (CPT) has been known to induce apoptosis in various cancer cell lines. To examine the intracellular apoptotic death signal initiated by CPT, we investigated the possible connection between caspase-3 activation and GSH depletion during CPT-induced apoptosis in HL-60 cells. Treatment of cells with $1{\;}{\mu}M$ CPT induced PARP cleavage accompanied by DNA fragmentation. z-VAD-fmk, a caspase-3 inhibitor, blocked the CPT-induced DNA fragmentation. Pretreatment of cells with N-acetylcysteine, a precursor of GSH biosynthesis, failed to inhibit CPT-induced PARP celavage and DNA gragmenatation. No significant changes in GSH depletion is not essential for caspase activation during CPT-induced apoptosis. We also investigated whether CPT-induced apoptosis is associated with changes of the levels of Bax and Bcl-2, two proteins involved in the control of apoptosis. Bcl-2 levels exhibited a late decrease compared with the kinetics of DNA fragmentation, whereas Bax levels increased more rapidly after CPT treatment. These results suggest that Bax plays more important role than Bcl-2 in inducing DNA fragmentation and may function upsteam of proteolytic activation of caspase-3 pathway in CPT-induced apoptosis.

• Journal of the Korea institute for structural maintenance and inspection
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• v.7 no.3
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• pp.231-239
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• 2003

Many foundation jobs have been designed and constructed in Korea with the sole information obtained from the SPT. The use of the CPT to estimate equivalent SPT values is becoming a common application for foundation design. The relationship between the CPT and the SPT has been determined in a number of studies over the past three decades. However a few papers have been published on the relationship between the CPT and the SPT in residual soil. Due to the large variation in published correlations between qt and N in residual soil, local correlations should be developed wherever possible. CPT tests have been carried out in the residual soil classified by SM in accordance with USCM and compared with SPT. Value of qt/N = 0.3 have been suggested for residual soil in Korea.

• Journal of Life Science
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• v.10 no.6
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• pp.621-629
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• 2000

Camptothecin (CPT) is an antitumor alkaloid that has been isolated from the Chinese tree, Camptotheca acuminata. The cytotoxicity of CPT has been correlated to its inhibition of DNA topoisomerase (Topo) I by stabilizing drug-enzyme-DNA “cleavable complex" resulting in DNA single-strand breaks and DNA-protein crosslinks. This studies were designed to elucidate whether CPT regulates Topo I mediated by CPT in DNAs containing c-myc protooncogene. We have conducted experiments on Topo I purification, pUC-MYC I cloning and Topo I assay using electrophoresis, quantitative RT-PCR and Northern blotting techniques. CPT ingibited the relaxation activity of Topo I in pUC19 DNA at various concentrations (1-1000 $\mu$M), while it enhanced the cleavage of Topo I in the pUC-MYC I by forming a cleavable complex at relatively high concentrations (100-1000 $\mu$M). In HL-60 cells treated with CPT, the expression of c-myc gene was decreased over that in the control group with no changes in the expression of Topo I mRNA. Our results suggest that Topo I is the target of CPT cytotoxicity but it does not affect Topo I extression, and the suppression of c-myc mRNA expression by CPT is due to c-myc damage resulted from formation of a cleavable complex with CPT. CPT.

• Proceedings of the Korean Geotechical Society Conference
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• 2008.10a
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• pp.792-798
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• 2008

CPT 시험은 지난 30여년 동안 지반조사 분야에서 널리 이용되어 왔다. CPT 콘의 근입은 항타말뚝의 근입방법과 유사하기 때문에, CPT 콘의 선단저항력을 이용하여 말뚝의 지지력을 산정하려는 연구가 많이 수행되어 왔다. 본 연구의 목적은 기존에 제안된 CPT 선단지지력 공식의 적용성을 분석하는 것이다. 이를 위해 낙동강 하구 대심도 연약지반에서 수행된 항타 PHC 말뚝에 대한 총 172개의 PDA 시험자료와 80개소의 CPT 자료를 수집하였다. PDA시험의 CAPWAP분석에서 얻어진 선단지지력과 각 CPT 지지력 공식에서 산정된 선단지지력을 비교함으로써 각 공식의 적용성을 분석하였다. 분석에 이용된 CPT 지지력 공식은 Aoki 방법, Meyerhof 방법, Penpile 방법, Philpponnat 방법, LCPC 방법, Schmertmann 방법, Zhou 방법, ICP 방법, Eslami & Fellenius 방법, 그리고 UWA-05 방법의 총 10가지이다. 분석결과, Aoki 방법, Phillipponnat 방법, ICP 방법 그리고 LCPC 방법 순으로 그 적용성이 높은 것으로 나타났다.

• Journal of the Korean Geotechnical Society
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• v.35 no.3
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• pp.37-46
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• 2019

Recently, the use of CPT-based liquefaction assessment method has increased by providing more accurate results than other field tests. In CPT-based liquefaction evaluation, various soil properties are predicted and they are used for liquefaction potential assessment. In particular, fines content is one of the important input parameters in CPT-based liquefaction assessment, so it is very important to use correct prediction model and to make quantitative evaluation of estimating variability of fines content. In this study, the error evaluation of existing models for prediction of fines content through CPT was performed, and the most suitable model was selected for Pohang area, where the liquefaction phenomenon was observed in the 2017. In addition, the inherent variability of soil was analyzed, and the estimating variability of fines content was evaluated quantitatively considering the inherent variability of soil, measurement error of CPT and transformation uncertainty of selected model.

• Journal of Korea Multimedia Society
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• v.3 no.6
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• pp.586-591
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• 2000

Color pictures on CPT are generally affected by the receiver characteristic and the condition of exterior illuminants. Moreover, readjustment of incorrect color in the CPT causes color distortion on a given picture. This paper describes a color adjustment method for modelling of CPT at the view point of observer in the ordinary lighting condition. To develop this method, we proposed ways of luminance recompensation under the gamma transformation for the intensity of illuminant, hue/saturation compensation for the types of illuminant and control of specific color for the reference. Based on these method, a color correlation system is suggested with the goal of reducing the color errors which are represented due to the influence of reflectance component of illuminants, the gamma transformation of CPT and the frequently adjustment of reference scene to set a optimum color.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
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• v.8 no.2
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• pp.273-286
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• 1997

Objective：This study was designed to evaluate effects of methylphenidate(MPH) on event-related potentials(ERP) and continuous performance test(CPT) in attention-deficit hyperactivity disorder (ADHD) and to see the correlation between changes in ERP and changes in performance. Method：ERP and CPT were used to examine the acute effects of MPH(0.5mg/kg) in eleven ADHD boys(89-103 months old). Results：1) After MPH administration, P3 latency to nontarget stimuli at Fz was significantly decreased (p<0.01) and P2 amplitudes to target stimuli at Pz and at Oz and P3 amplitude to target stimuli at Cz were significantly increased(p<0.05). 2) Commission error and omission error in the CPT-X and commission error in the CPT-AX were decreased(p<0.01), and hits and perceptual sensitivity(d') in the CPT-X and d′ in the CPT-AX were increased(p<0.01). 3) The change of P3 latency to nontarget stimuli at Fz and the change of d′ in the CPT-X were negatively correlated(p<0.05), and the change of P2 amplitude to target stimuli at Pz and d′ in the CPT-AX were positively correlated(p<0.05). Conclusion：MPH improves change orienting reaction, the delivery of task relevant information, accuracy and perceptual sensitivity in ADHD. And the increase of ability to discriminate targets from non-targets reflects reduced evaluation time in large memory component task and enhanced change orienting reaction in simple task.

• Journal of the Korean Society of Food Science and Nutrition
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• v.28 no.3
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• pp.670-676
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• 1999

The effect of exercise and/or high fat diet on carnitine status and carnitine palmitoyltransferase I(CPT I) level were investigated in Weanling Sprague Dawley rats. The rats were fed an AIN 76 diet or a modified high fat AIN diet, supplemented with 35% corn oil, for 31 days. During the 31 day period half of the animals in each dietary group were exercised on a treadmill for 90 minutes per day. Carnitine concentrations were determined in plasma and liver and CPT I mRNA levels were measured by Northern blot analysis with CPT I cDNA probe in livers of rats. Exercise rats gained less weight than non exercised rats during the study for high fat diet group. Exercise rats had a higher plasma acid soluble acylcarnitine and acid insoluble acylcarnitine concnetrations than non exercised rats for normal diet group. Exercise or high fat diet increased liver carnitine concentration, but a mixed effect was not shown. In exercised rats, CPT I mRNA levels increased significantly relative to those of nonexercised rats. CPT I mRNA levels also increased when compared high fat fed rats with those of normal diet fed rats. These data suggest that there is a correlation between carnitine concen trations and CPT I mRNA levels and that CPT I can be regulated at the transcriptional level by exercise and/or high fat diet.

• Journal of the Korean Geotechnical Society
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• v.25 no.12
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• pp.107-118
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• 2009

As CPT penetration tends to show a similar behavior to that of pile driving, a number of methods for estimating the toe bearing capacity of piles based on CPT data have been proposed. To evaluate the applicability of the methods in this country, a total of 172 dynamic load tests data on PHC piles and 82 CPT data at a site in the Nakdong River estuary were collected. A specific four-step procedure was adopted for the selection of the reliable data, and statistical techniques were then applied to the analysis of the applicability. The results indicated that among a total of 10 CPT-based methods applied, the best one is the Aoki method (1975), followed by the LCPC (1982), ICP (2005) methods and others.

• Proceedings of the Korean Geotechical Society Conference
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• 1998.10a
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• pp.445-452
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• 1998