• Natural Product Sciences
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• v.15 no.4
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• pp.229-233
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• 2009

The essential oil fraction was obtained from the leaves and flowers of Artemisia iwayomogi (Compositae) by steam distillation, and its main component, vulgarone B, was isolated by column chromatography. RAW 264.7 cells were used to investigate the anti-inflammatory properties of A. iwayomogi and vulgarone B. Cell viability was determined by MTT assay after treatment with various dilutions of the compounds. In addition, several assays were used to determine the effects of A. iwayomogi essential oil components on immune stimulation. Nitric oxide production in cells activated with lipopolysaccharide (LPS) was evaluated by reaction with Griess reagent. Both vulgarone B and the essential oil fraction of A. iwayomogi inhibited the production of nitric oxide. The effects on various cytokines released from the cells were also measured using ELISA. The production of prostaglandin $E_2$ was significantly decreased by treatment with A. iwayomogi oils. LPS-induced IL-$1{\beta}$ and IL-6 production were also decreased in a dose-dependent manner, but no significant effect on TNF-${\alpha}$ was observed at the concentrations tested. Finally, Western blot analysis revealed that A. iwayomogi oils reduced the levels of COX-2 and iNOS.

• Research in Plant Disease
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• v.21 no.1
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• pp.1-5
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• 2015

A PCR method has been developed for the pathovar-specific detection of Pseudomonas syringae pv. tagetis, which is the causal agent of bacterial leaf spots and apical chlorosis of several species within the Compositae family. One primer set, PSTF and PSTR, was designed using a genomic locus derived from an amplified fragment length polymorphism (AFLP) fragment produced a 554-bp amplicon from 4 isolates of P. syringae pv. tagetis. In DNA dot-blot analysis with the PCR product as probe, a positive signal was identified in only 4 isolates of P. syringae pv. tagetis. These results suggest that this PCR-based assay will be a useful method for the detection and identification of P. syringae pv. tagetis.

• Journal of Pharmaceutical Investigation
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• v.41 no.6
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• pp.377-380
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• 2011

The Artemisia princeps (Compositae) has been used in traditional Korean medicine for the treatment of microbial infections and inflammatory diseases. Since A. princeps is generally difficult to be discriminated from A. capillaris, A. caplillaris has been misused in place of A. princeps. To solve this problem, a rapid and nondestructive method for discrimination of A. princeps and A. capillaris samples was developed using near infrared spectroscopy (NIRS) in the present study. A principal component analysis (PCA) and a partial least squares discrimination analysis (PLS-DA) were performed to discriminate two species. As a result, with the use of PLS-DA, A. princeps and A. capillaris were clustered according to their genus. These outcomes indicated that the NIRS could be useful for the discrimination between Artemisia princeps and Artemisia capillaris.

• Journal of Species Research
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• v.6 no.1
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• pp.76-90
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• 2017

Aster altaicus var. uchiyamae Kitam. is an endemic taxon of Korea and is protected by law as an endanger taxon. The genetic information of A. altaicus var. uchiyamae is unavailable in Genbank. Here we sequenced chloroplast genome of A. altaicus var. uchiyamae. The cp-genome of Aster altaicus var. uchiyamae was 152,446 bps in size: LSC was 84,240 bps, IR 25,005 bps, SSC 18,196 bps. The cp-genome contains 112 genes and 21 introns consisted of 79 protein coding genes(PCGs), 4 RNA genes, and 29 tRNA genes, with 20 group II introns and one group I intron. There were three pseudo-genes including ${\psi}$-ycf1, ${\psi}$-rps19, and ${\psi}$-trnT_GGU. Eighteen genes, five introns, and parts of two genes and an intron are found within the IR, which has two copies. The cp-DNA of Aster altaicus var. uchiyamae is distinguished from A. spathulifolius, only known cp-genome of the genus Aster, by 172 SNP in genic regions of 43 PCGs and 21 indels in 11 PCGs and SSU. The chloroplast genome sequence was deposited at GenBank (KX35265).

• Korean Journal of Plant Resources
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• v.1 no.1
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• pp.53-71
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• 1988

The plants medicinal resources of Mt. Baegun were investigated 21 times from March 1, 1986 to December 30, 1987. In order to analyze the vegetation of Mt.Baegun area, herb plants structure and distribution. Herb plants of Baegun Mt.consisted of 60 families, 250 species in all, The resourees of important herb drugswere Campanulaceae, Salicaceae, Moraceae, Polygonaceae. Amarantaceae, Plant-aglnaceae, Labiatae, Compositae, Asclepiaclaceae, Leguminosae, Gentianaceae, Liliaceae, lilicaceae, Dioscoreaceae, Caprifollaceae, Ranunculaceae, Lauraceae, LardicaDalaceae, Araliaceae, Solanaceaer Cornaceae, Fagaceae and Rosaeeae. Theherb durgs were eomparatively more than in other mountains in our country.

• Korean Journal of Plant Resources
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• v.1 no.1
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• pp.23-32
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• 1988

The Plants medicinal resources of Mt. Mohu were investigated 12 times from July 1, 1987 to July 28, 1988. In order to analyze the vegetation of Mt. Mohu area,herb plants structure and distribution. Herb plants of Mohu Mt. consisted of 58families, 230 species in all. The resources of important herb drugs were plant-aginaceae, Labiatae, Amarantaceae, Campanulaceae, Asclepiaclaceae, Leguminosae,Gentianaceae, Liliaceae, lilicaceae, Dioscoreaceae, Compositae, Caprifoliaceae,Ranuncvlaceae, Lauraceae, Lardizabalaceae, Araliaceae, Solanaceae, Cornaceae,Fagaceae and Rosaceae. The herb drugs were comparatively more than in othermountains in our country.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2011.10a
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• pp.19-19
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• 2011

예비실험 중 한국의 울릉도에 자생하고 취나물로 이용되고 있는 섬쑥부쟁이(Aster glehni Franchet et Sckmidt, Compositae)가 페놀성 화합물 함량이 높고 peroxynitrite 소거효과가 높았기 때문에 이를 이용한 추출물의 제조를 통한 건강기능식품을 창출하고자 하였다. 건강식품을 위한 추출물 제조를 위하여 물과 에탄올만을 이용하여 페놀성 화합물 고함유 추출물의 제조가 가능하고 높은 peroxynitrite 소거효과를 나타내는 추출물을 결정하고자 하였다. 즉, 추출용매로 Water-EtOH 비율을 조절하여 추출하여 추출물의 함량, HPLC를 이용한 caffeoylquinic acid(CQ) 화합물의 함량분석 및 peroxynitrite 소거효과를 비교하였다. Water-EtOH (7:3)으로 추출한 섬쑥부쟁이 추출물은 CQ 함량이 높고, 높은 peroxynitrite 소거효과, 항경련효과, 진정효과 및 항비만효과 등이 나타났다. 건강기능식품을 위한 추출물 제조시 한국에서는 물과 에탄올만이 허용되기 때문에 CQ 함량이 높은 추출물의 제조가 가능하고 높은 peroxynitrite 소거효과를 유지할 수 있는가에 관한 실험 이외에 마우스에서 선택된 추출물의 진정효과 및 항경련효과 실험을 수행하였다. 진정효과와 항경련효과를 위하여 마우스에서 각각 pentobarbital로 유도한 수면연장효과와 PTZ로 유도한 경련에 대한 효과를 측정하여 유의성있는 활성이 확인되었으므로 이 추출물을 이용한 건강기능식품으로의 이용이 가능하다고 생각된다.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.66-66
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• 2018

국화과(Compositae) 다년생 초본인 산국속(Dendranthema)은 국내 약 13여종이 자생하는 것으로 알려져 있으며, 이 중 감국(D. indicum (L.) Des Moul.)과 산국(D. boreale (Makino) Ling ex Kitam.), 구절초(D. zawadskii var. latilobum (Maxim.) Kitam.)가 주로 차 또는 한약재 등의 원료로 이용되고 있다. 차로 이용되는 꽃은 산국이 감국에 비해 상대적으로 작아서 구분이 가능하지만 시중에는 건조된 형태로 가공 유통되므로 육안으로 구분이 쉽지 않고, 산국 유래 제품들은 국내에서 감국 또는 국화로 혼용해서 표기되어 유통되고 있어 그 기원을 명확히 정립할 필요가 있다. 이에 본 연구는 감국과 산국의 분자유전학적 판별을 위해 DNA 바코드 후보 유전자를 활용하여 염기서열분석으로 확보된 SNP 및 InDel 정보를 바탕으로 CAPS 마커를 개발하고자 수행되었다. 감국과 산국 모두 trnL-trnF intergenic spacer 구간에서 약 1kb의 PCR 산물이 확인되었고, 이들 염기서열에서 분석한 2 SNP 및 3 InDel을 대상으로 CAPS 마커 개발을 위한 제한효소 사이트를 탐색하였다. Gap을 포함한 774bp (감국/산국=A/G) 위치의 SNP에서 BstUI(GC^GC)처리로 CAPS 마커로 전환 가능함이 확인되었고, 이에 감국과 산국의 PCR 산물에 제한효소를 처리한 결과, 제한효소 인식 사이트가 존재하는 산국에서 두 개의 DNA 단편이 확인되었다. 위 결과는 다양한 형태로 가공 유통되는 감국과 산국의 판별을 위한 마커로 활용될 수 있으며, 본 연구에 활용된 기술은 추후 건강기능식품 개발을 위한 원료표준화 확립 연구에 유용할 것으로 판단된다.

• Korean Journal of Medicinal Crop Science
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• v.3 no.2
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• pp.151-155
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• 1995

Chromatographic separation of Laduca raddeana extract afforded a mixture of fatty acyl triterpene, triterpene acetates and primary long chain alcohol. The kind of triterpene moieties in these two triter­penoids was six, i.e., ${\beta}-amyrin,\;{\alpha}-amyrin$, lupeol, pseudotaraxasterol, taraxasterol and germanicol on the basis of chemical and spectroscopic methods. The acyl moieties in the corresponding acyl mixture were characterized as acetates, myristate, palmitate, stearate and arachidate. And a mixture of primary long chain alcohol were composed of teracosanol and hexacosanol.

• Natural Product Sciences
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• v.24 no.2
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• pp.109-114
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• 2018

Artemisia capillaris Thunb. (Compositae) is a native herb of East Asian countries and has used for the treatment of jaundice, high liver fever, and digestive diseases for a long time, as well as being developed as the source of herbal preparations until now. The major components from A. capillaris were chlorogenic acid (1) and its derivatives substituted with caffeoyl moieties, such as 3,5-dicaffeoylquinic acid (2) and 4,5-dicaffeoylquinic acid (3), and coumarins, such as scoparone. In the study, four compounds, chlorogenic acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid and scoparone (4) in the 70% ethanolic extract of A. capillaris were simultaneously determined by using HPLC-UVD system. This method was validated with the terms of linearity, precious and accuracy according to ICH guidelines. The developed method was successfully applied for the quantitative analysis of Artemisia genus, A. capillaris, A. iwayomogi, A. princeps, and A. argyi, distributed in Korea.