• Ocean and Polar Research
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• 제33권2호
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• pp.127-133
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• 2011

The comet assay, also called single-cell electrophoresis (SCGE) assay, is a potential sensitive monitoring tool for DNA damage in cells. The primary objective of this study was to use comet assay to ascertain if the blood cells of flounder (Pleuronichthys olivaceus) and muscle cells of clam (Saxidomus purpurata) are suitable for genotoxicity screening. This was achieved by initially exposing blood and muscle cells under in vitro conditions to the reference genotoxin hydrogen peroxide ($H_2O_2$); strong correlation between $H_2O_2$ concentration and comet values were found. Subsequently, the identification of DNA damage in isolated cells from flounder and clam was performed under in vivo exposure to benzo(a)pyrene (BaP) and tributyltin (TBT). Flounder and clam were exposed to different concentrations (1, 10, 50, 100 ${\mu}g/L$) of BaP or TBT for 4 days. Regardless of treated chemicals, blood cells of flounder were more prone to DNA breakage compared to muscle cells of clam. In conclusion, in vivo genotoxicity of BaP and TBT can be biomonitored using the comet assay. This study suggests that flounder and clam do show potential as mediums for monitoring genotoxic damage by comet assay.

• Journal of Astronomy and Space Sciences
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• 제31권3호
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• pp.215-223
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• 2014

In this paper, we report the analysis of Korean historical records on the periodic Halley's comet according to the period (i.e., the Three Kingdoms, Goryeo Dynasty, and Joseon Dynasty) using various sources such as the Samguksagi (The History of the Three Kingdoms), Goryeosa (The History of the Goryeo Dynasty), and Joseonwangjosillok (The Annals of the Joseon Dynasty). With regards to the apparition time of the comet for each return, we referred to the works of Kronk. For the Three Kingdoms period, we could not find any record relevant to Halley's comet from the Samguksagi. Furthermore, we examined the suggestion that the phenomenon of "two Suns" which appeared on April 1, 760 (in a luni-solar calendar), as recorded in the Samgukyusa (The Legends and History of the Three Kingdoms), indicates an instance of the the daytime appearance of Halley's comet. In contrast with the Three Kingdoms period, we found that all returns of Halley's comet are recorded during the Goryeo Dynasty, although others have questioned some accounts. We also found that the appearance of Halley's comet in 1145 is mentioned in a spirit-path stele made in 1178. For the Joseon Dynasty period, we found that all apparitions of the comet are recorded, as with the Goryeo Dynasty, except for the return of 1910, at which time the former dynasty had fallen. In conclusion, we think that this study will be helpful for understanding Korean historical accounts on Halley's comet.

• International Union of Geodesy and Geophysics Korean Journal of Geophysical Research
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• 제22권1호
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• pp.6-23
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• 1994

A disconnection event (DE) of the cometary plasma tail is one of most spectacular phenomena observed in comets. Yet, for years it has remained one of the great unsolved problems I astronomy and space physics. The solar wind is thought to play a major role in the creation of comet plasma tail (type Ⅰ) disconnection events. The goal of this paper is to present a mechanism that explains the disconnection event in terms of the local conditions at the comet. Comparison of the solar wind conditions and 16 DEs in Halley's comet shows that DEs are associated primarily with crossings of the heliospheric sector boundary and apparently not with any other properties of the solar wind, such as a high speed stream[Yi et al., 1994]. A 3-dimensional resistive magnetohydrodynamic simulation in this paper supports this association by showing that only front-side magnetic reconnection between the reversed interplanetary magnetic fields that exist when a comet crosses the heliospheric sector boundary [Niedner and Brandt, 1978] could reproduce the morphology of a DE, including ray formation [Brandt, 1982].

• Journal of Nutrition and Health
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• 제35권2호
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• pp.213-222
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• 2002

The alkaline comet assay has been used with increasing popularity to investigate the level of DNA damage in biomonitoring studies within the last decade in Western countries. The purpose of this study was to evaluate the usefulness of the alkaline comet assay as a biomarker of oxidative DNA damage for monitoring in the Korean population, and also to evaluate the effect of nutritional status and lifestyle factors on H2O2 induced oxidative DNA damage measured by the alkaline comet assay in human lymphocytes. The study population consisted of 61 healthy Korean male volunteers, aged 20-28. Epidemiological background data including dietary habits, smoking habits and anthropometrical measurements were collected through personal interviews. After blood collection, the comet assay in peripheral lymphocytes and plasma lipids analysis was carried out and the results analyzed. Tail moment (TM) and tail length (TL) of the comet assay were use\ulcorner to measure DNA damage in the lymphocytes of the subjects. Statistically significant (p < 0.05) positive correlations were observed between DNA damage (TM or TL) and smoking habits expressed as cigarettes smoked per day and pack years (r = 0.311 and 0.382 for TM, r = 0.294 and 0.350 for TL, respectively). There were also significant positive correlations between DNA damage parameter and waist-hip ratio. Higher plasma triglyceride levels were associated with increased damage to DNA. There were no correlations between the consumption frequencies of vegetables and DNA damage to the subjects. However, consumption frequencies of fruit and fruit juice intake were inversely associated with the TM and TL. The results indicate that die comet assay is a simple, rapid and sensitive method for detecting lymphocyte DNA damage induced by cigarette smoking. Consumption of fruit or fruit juices could potentiall modify the damaged DNA in the human peripheral lymphocytes of young Korean men.

• 한국환경독성학회:학술대회논문집
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• 한국환경독성학회 1997년도 제20회 화학물질의 환경독성과 건강영향
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• pp.101-101
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• 1997

Comet assay, single cell gel electrophoresis has been known as useful, rapid, simple, visual, and sensitive technique for measuring the DNA breakage in mammalian ce1ls. For evaluation of DNA damage using comet assay, early studies reported a change in comet length and intensity with DNA damage using simple visual technique, such as fluorescence microscopy with eyespiece. In recent, some workers are observing and analyzing nucleotide of comets using quantitative fluorescence image analysis system to estimate 'tail moment', which is defined as the product of the tail length and the fraction of total DNA in tail. Our laboratory also adopted the image analysis software for qualification. In addition, many of the practical features of comet assay render it potentially attractive as useful tool for molecular toxicology and carcinogenesis, because the system is already showing considerable promise as rapid predictor in both in vitro and in vivo experimental designs. Recently, the comet assay becomes a attractive technique to study of apoptosis, because apoptotic fragmentation of nuclear DNA into nucleosomal sizes can be evaluated by the comet assay. So, we attempted to apply the comet assay to studying the effect of various stress on the apoptosis-sensitive cell lines. Particularly, focusing on the hyperthermic apoptosis, we could find that heat shock(44˚C for 60 minutes) was sufficient to induced apoptosis in these cell lines. But using the highly sensitive comet assay, we could not detect DNA breaks immediately after heat shock.

• 한국작물학회지
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• 제36권3호
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• pp.209-213
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• 1991

조숙옥수수를 포함한 사료작물 작부체계 구성시 조숙옥수수의 생산성을 확보하기 위해 만파시밀식재배를 시도하였다. 조숙옥수수로는 Comet80, Comet85, Linda 품종을 이용하였고 만파정도는 수원19호 적기보다 45, 55, 65일 만큼 늦게 파종하였다. 파종밀도는 각 파종기에 대하여 60$\times$20, 50x20, 40$\times$20cm로 밀식정도를 달리하였다. 각처리구의 수확은 8월 29일 일괄실시 하였는데 조숙옥수수의 이러한 처리에 따른 수량구성특성 변화와 조숙옥수수의 촉과작물 작부체계도입 가능성을 조사한 결과를 요약하면 다음과 같다. 1. 6월 22일까지 파종에서 건물수량은 Comet85, 수원19호 Comet80, Linda 순이었다. 2. 6월 12일까지의 파종에서 수원19호의 이삭비율은 6.7%이었는데 비해 Comet80, Cometss, Linda의 평균 이삭 빈율은 40.3%이었다 3. 조숙품종의 밀식에 의한 건물수량 보상효과정도는 6월 22일까지 파종에서 20x50cm밀도가 20$\times$60cm밀도에서 보다 평균 37.3% 증수하였다. 4. 만파시 Comet85는 수원19호보다 평균 24.1 % 많은 TDN을 생산하였다.

• Journal of Astronomy and Space Sciences
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• 제31권1호
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• pp.63-66
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• 2014

Since Rigollet first discovered a comet in 1939, many follow-up observations have been made, particularly in Europe. It is now known that the Rigollet comet is identical with the one observed by Herschel in 1788, and thus it is now called 35P/1939 O1 or the Herschel-Rigollet comet. Yumi, a Japanese astronomer, also observed the Rigollet comet in Korea using a 6-inch refractor telescope, and published his data in two Japanese journals (Bulletin of the Observatory of the Government-General of Korea and Publication of the Lecture on Meteorology). In his paper, Yumi also referred to observations by Hirose and Kanda in Japan. However, their works have not been given attention by international society. In this study, we analyze the observation data of Yumi and present preliminary orbital elements using it with a modified Gauss method. We expect that this study will be used to refine the orbital elements of the Rigollet comet by orbital-calculation experts. For that reason, we have also transcribed all the observational data presented by Yumi.

• 한국식품과학회지
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• 제32권4호
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• pp.747-754
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• 2000

Comet assay를 이용하여 방사선 조사육의 조사여부 및 조사량을 판별해내는 방법을 개발하기 위해 1-10 kGy의 감마선 조사량으로 조사한 육조직에서 일어나는 DNA 손상을 측정하였다. Comet assay에 있어 최적의 comet image를 얻기 위해 세포 분리, 세포 lysis 및 전기영동에 대한 여러 조건들을 적용하여 최적의 조건을 확립하였다. DNA 손상도는 관찰되는 comet의 평균 tail length와 tail length에 의해 구분된 4 손상 등급에의 분포, 그리고 그 분포비율에 의해 본 연구에서 제시한 식에 의해 계산한 relative damage index(RDI) 값 등으로 비교 판정하였다. 평균 tail length와 RDI 값은 조사량이 증가함에 따라 증가하여 DNA 손상도가 증가됨을 나타내었으며, 평균 tail length으로는 조사량 간의 차이를 명확히 분별하기가 어려웠던 반면 RDI 값에 의하면 조사여부 및 조사량을 판별하기에 적합함을 알 수 있었다. 국내산 한우육과 수입 냉장육에 대하여 blind test를 실시한 결과 수입육이 높은 DNA 손상도를 나타내었는데 그 RDI 값은 방사선 조사에 의한 값보다는 비교적 낮은 것이어서 수입육의 DNA 손상은 방사선 조사가 아닌 저온처리의 결과인 것으로 판단되었다. 본 연구의 결과로부터 Comet assay가 우육의 방사선 조사여부 및 조사량 판별에 유용한 기술로 응용될 수 있을 것으로 판단되었다.

• 한국식품과학회지
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• 제34권1호
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• pp.18-23
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• 2002

방사선 조사된 대두의 검지방법을 연구하기 위하여 국산 및 중국산 대두에 대하여 $0.5{\sim}4.0\;kGy$의 감마선을 조사하고 열발광(TL), 전자스핀공명(ESR) 및 DNA comet 특성을 비교 검토하였다. TL 측정에서 비조사구는 $280^{\circ}C$ 부근에서 매우 낮은 glow curve를 나타내었고, 감마선 조사구는 $200^{\circ}C$ 부근에서 조사선량에 의존적인 glow curve를 나타내었다. 재조사(re-irradiation) 방법에 의한 TL ratio$(TL_1/TL_2)$의 비교는 TL 측정 결과의 정확도를 높여 주었다. 원산지별 TL 특성 비교에서 국산을 중국산에 비해 높은 TL intensity를 나타내었다(p<0.01). 대두 껍질을 사용한 ESR 측정에서는 조사시료에서 cellulose radical 유래의 특이한 signal(g = 2.02374, 1.98715)을 보여주었고 중국산 시료의 ESR signal이 더 높게 나타났다. 비조사 대두의 DNA comet은 tail이 없거나 아주 짧은 tail을 가진 전형적인 intact cell을 나타내었다. 그러나 0.5 kGy 이상의 감마선 조사 시료에서는 조사선량에 의존적으로 comet의 tail length 증가와 더불어 comet의 크기 및 농도의 변화가 일어났다. 이상의 결과에서 TL, ESR 및 DNA comet의 분석은 대두의 방사선 조사 여부 확인을 가능하게 하였으며, 원산지별 검지특성의 차이는 거의 없었다.

• Journal of Plant Biotechnology
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• 제31권3호
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• pp.225-230
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• 2004

방사선에 노출된 고추유묘의 잎으로부터 세포핵을 분리하여 단세포전기영동방법인 comet 분석을 통하여 핵 DNA의 손상정도를 조사하였다. Comet 분석에서 꼬리부분의 길이 (T)와 머리부분의 길이 (H)를 측정하여 T/H 비율을 조사하였다. 무처리세포는 T/H 비율이 1.28이었으나 50 및 100 Gy의 방사선을 처리한 세포는 각각 3.54 및 3.39로 방사선처리에 의해 상당량의 핵 DNA가 손상을 입은 것으로 나타났다. Comet의 head-DNA량은 무처리가 76.8%였으나 50 및 100 Gy를 처리한 세포는 각각 55.9% 및 59.5%를 보였다. 고선량의 방사선을 처리하기 전에 미리 20 Gy 이하의 저선량 방사선을 종자에 전처리하였을 경우 종자의 발아 및 생장에 대한 영향은 없었지만, 후속 고선량에 대한 핵 DNA의 손상은 경감되는 경향을 보였다.