• 대한전기학회:학술대회논문집
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• 대한전기학회 1995년도 하계학술대회 논문집 C
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• pp.1242-1244
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• 1995

The dielectric and polarizable properties of $0.9Pb_{1-x}La_x({Mg}_{1/3}Nb_{2/3})O_3-0.1PbTiO_3$ (x=0,1,2,3,4,5) have been investigated. The temperature-dependant electrostictive characteristics of 0.9PMN-0.1PT relaxor ferroelectric system were improved by enhencing the extent of the diffuse phase transition(DPT). This was achieved using PMN-PTceramics by the partial substitution of La at the Pb site. The curie temperature and the maximum dielectric permittivity decreased by substituting La and the electric field-related hysteresis phenomena decreased with increasing La substitution amount.

• Journal of Periodontal and Implant Science
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• 제48권1호
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• pp.34-46
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• 2018

Purpose: The purpose of this study was to evaluate the effects of 1,25-dihydroxyvitamin $D_3$ on the proliferation, differentiation, and matrix mineralization of MC3T3-E1 osteoblast-like cells in vitro. Methods: MC3T3-E1 osteoblastic cells and 1,25-dihydroxyvitamin $D_3$ were prepared. Cytotoxic effects and osteogenic differentiation were evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, alkaline phosphatase (ALP) activity assay, ALP staining, alizarin red S staining, and reverse transcription-polymerase chain reaction (RT-PCR) for osteogenic differentiation markers such as ALP, collagen type I (Col-I), osteocalcin (OCN), vitamin D receptor (VDR), and glyceraldehyde 3-phosphate dehydrogenase. Results: The MTT assay showed that 1,25-dihydroxyvitamin $D_3$ did not inhibit cell growth and that the rate of cell proliferation was higher than in the positive control group at all concentrations. ALP activity was also higher than in the positive control group at low concentrations of 1,25-dihydroxyvitamin $D_3$ ($10^{-10}$, $10^{-12}$, and $10^{-14}M$). RT-PCR showed that the gene expression levels of ALP, Col-I, OCN, and vitamin D receptor (VDR) were higher at a low concentration of 1,25-dihydroxyvitamin $D_3$ ($10^{-12}M$). Alizarin red S staining after treatment with 1,25-dihydroxyvitamin $D_3$ ($10^{-12}M$) showed no significant differences in the overall degree of calcification. In contrast to the positive control group, formation of bone nodules was induced in the early stages of cell differentiation. Conclusions: We suggest that 1,25-dihydroxyvitamin $D_3$ positively affects cell differentiation and matrix mineralization. Therefore, it may function as a stimulating factor in osteoblastic bone formation and can be used as an additive in bone regeneration treatment.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 1994년도 춘계학술대회 논문집
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• pp.1-4
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• 1994

In this study. the microwave dielectric properties, such at dielectric constant(${\varepsilon}_{\gamma}$), unloaded quality factor multiplied with resonant frequency($Q_{u}$.f), and temperature coefficient of resonant frequency(${\tau}_{f}$), were investigated for the low-temperature sintered (Pb, Ca)$ZrO_3$ with $V_2O_{5}$ additives as well as structural properties. As a result, (Pb,Ca)ZrO$_3$ with 0.2 wt% $V_2O_{5}$ additive, sintered at $1200^{\circ}C$, showed good properties like ${\varepsilon}_{\gamma}$ 109, $Q_{u}$.f 2736 GHz, and ${\tau}_{f}$ -2.94 ppm/$^{\circ}C$. Especially ${\tau}_{f}$ was much better than ($Pb_{0.63}Ca_{0.37}$)$ZrO_3$ of which ${\tau}_{f}$ was known to be + 13.4 ppm/$^{\circ}C$, so it seems to be applicated in micorowave device components.

• Molecular & Cellular Toxicology
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• 제3권2호
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• pp.137-144
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• 2007

The mechanism of cytotoxicity of doxifluridine, a prodrug fluorouracil (5-FU), has been ascribed to the misincorporation of fluoropyrimidine into RNA and DNA and to the inhibition of the nucleotide synthetic enzyme thymidylate synthase. Increased understanding of the mechanism of 5-FU has led to the development of strategies that increases its anticancer activity or predicts its sensitivity to patients. Using GeneChip?? Rhesus Macaque Genome arrays, we analyzed gene expression profiles of doxifluridine after two weeks repeated administration in cynomolgus monkey. Kegg pathway analysis suggested that cytoskeletal rearrangement and cell adhesion remodeling were commonly occurred in colon, jejunum, and liver. However, expression of genes encoding extracellular matrix was distinguished colon from others. In colon, COL6A2, COL18A1, ELN, and LAMA5 were over-expressed. In contrast, genes included in same category were down-regulated in jejunum and liver. Interestingly, MMP7 and TIMP1, the key enzymes responsible for ECM regulation, were overexpressed in colon. Several studies were reported that both gene reduced cell sensitivity to chemotherapy-induced apoptosis. Therefore, we suggest they have potential as target for modulation of 5-FU action. In addition, the expression of genes which have been previously known to involve in 5-FU pathway, were examined in three organs. Particularly, there were more remarkable changes in colon than in others. In colon, ECGF1, DYPD, TYMS, DHFR, FPGS, DUT, BCL2, BAX, and BAK1 except CAD were expressed in the direction that was good response to doxifluridine. These results may provide that colon is a prominent target of doxifluridine and transcriptional profiling is useful to find new targets affecting the response to the drug.

• Journal of Nutrition and Health
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• 제55권6호
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• pp.617-629
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• 2022

본 연구는 속단으로부터 단일 화합물로 분리한 Hed의 조골세포 활성 효능과 작용 기전 규명을 위해 수행되었다. Hed는 MC3T3-E1 세포의 증식과 ALP 효소 활성을 자극하여 세포외 기질에 인산과 칼슘 이온 침착을 증가시켰다. Hed는 BMP-2/Smad 신호 전달 경로를 활성화하여 Runx2, ALP, OPN 및 ProCOL의 mRNA와 단백질 발현을 유의하게 증가시켜 조골세포의 성숙과 분화를 유도하였다. 따라서 본 연구결과 Hed는 조골세포 활성 효능을 가진 것으로 판단되며 항골다공증 기능성 소재로의 개발 가능성을 확인하였다.

• Preventive Nutrition and Food Science
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• 제21권4호
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• pp.310-316
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• 2016

In this study, the anti-osteoarthritis effects of Cynanchum wilfordii, Phlomis umbrosa, and Angelica gigas extract (CPAE), observed and confirmed in previously clinical studies were further investigated by in vitro and in vivo studies. Anabolic biomarkers related to healthy cartilage maintenance, such as aggrecan, type II collagen ${\alpha}$-1 (Col2a1), sex determining region Y-box-9 (Sox-9), and catabolic biomarkers related to osteoarthritis, such as cyclooxygenase-2 (Cox-2), matrix metalloproteinase-13 (Mmp13), and nuclear factor kappa-light-chain-enhancer of activated B cells ($Nf{\kappa}b$), were evaluated by quantitative reverse transcriptase polymerase chain reaction and reporter gene assay. In vitro study results showed significant changes in both anabolic and catabolic biomarkers. For anabolic factors, significant changes in the level of aggrecan (P<0.05), Col2a1 (P<0.05), and Sox-9 (P<0.01) activation were shown after treatment of cartilage cells with CPAE (50 ng/mL) with similar efficacy compared to insulin growth factor, the positive control (100 ng/mL). For catabolic factors, significant changes in the inhibition activity of Cox-2 (P<0.05), Mmp13 (P<0.01), and $Nf{\kappa}b$ (P<0.05) were shown for CPAE (50 ng/mL) with similar efficacy compared to Celecoxib, the positive control ($10{\mu}M$). In the in vivo carrageenan-induced paw edema model study results showed that CPAE-treated groups (100 mg/kg) and Celecoxib-treated groups (60 mg/kg) showed comparably significant efficacy of inhibition by 37.1% and 52.1%, respectively. Furthermore, CPAE (200 mg/kg) showed similar effect to Celecoxib (60 mg/kg) with an inhibition rate of 54.3%. This result confirms that CPAE effectively inhibited the inflammation-induced osteoarthritis symptoms.

• The Plant Pathology Journal
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• 제36권3호
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• pp.255-266
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• 2020

Plant immune responses can be triggered by chemicals, microbes, pathogens, insects, or abiotic stresses. In particular, induced systemic resistance (ISR) refers to the activation of the immune system due to a plant's interaction with beneficial microorganisms. The phenolic compound, 2,4-diacetylphloroglucinol (DAPG), which is produced by beneficial Pseudomonas spp., acts as an ISR elicitor, yet DAPG's mechanism in ISR remains unclear. In this study, transgenic Arabidopsis thaliana plants overexpressing the DAPG hydrolase gene (phlG) were generated to investigate the functioning of DAPG in ISR. DAPG was applied onto 3-week-old A. thaliana Col-0 and these primed plants showed resistance to the pathogens Botrytis cinerea and Pseudomonas syringae pv. tomato DC3000. However, in the phlG transgenic A. thaliana, the ISR was not triggered against these pathogens. The DAPG-mediated ISR phenotype was impaired in transgenic A. thaliana plants overexpressing phlG, thus showing similar disease severity when compared to untreated control plants. Furthermore, the DAPG-treated A. thaliana Col-0 showed an increase in their gene expression levels of PDF1.2 and WRKY70 but this failed to occur in the phlG transgenic lines. Collectively, these experimental results indicate that jasmonic acid/ethylene signal-based defense system is effectively disabled in phlG transgenic A. thaliana lines.

• 농업과학연구
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• 제10권2호
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• pp.249-256
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• 1983

1경(莖) 4화(花)의 백합절화(百合切花)에 있어서 각(各) 화(花)의 개화양상(開花樣相)과 절화보존용액(切花保存溶液)의 효과(效果)를 조사(調査)하였던 바 다음과 같은 결과(結果)를 얻었다. 1. 제(第)1화(花)는 절화보존(切花保存) 후(後) 1~2일(日), 2화(花)는 3~4일(日), 3화(花)는 5~6일(日)이면 개화(開花)하여, 1, 2, 3화(花)가 동시관상(同時觀賞)이 가능(可能)하였으나, 제(第)4화(花)는 1, 2화(花)의 수명(壽命)이 끝날 무렵인 8~9일(日)이 지나서야 개화(開花)하였다. 보존용액(保存溶液)들 간(間)에서 각(各) 화(花)의 개화시기차이(開花時期差異)는 인정(認定)되지 않았다. 2. 한줄기에 있어서 각(各) 화(花)의 수명(壽命)은 제(第)1화(花)에서 4화(花)로 갈수록 수명(壽命)이 짧았고 보존용액(保存溶液)의 수명연장효과(壽命延長效果)는 4화(花)에서 1화(花)로 갈수록 크게 나타났다. 3. 절화(切花)한 다음 수일(數日) 후(後)에 개화(開花)하게 되는 제(第)3화(花) 및 4화(花)는 sucrose와 $AgNO_3$ 또는 HQ가 혼합(混合)된 용액(溶液)에 보존(保存)하였을 때 제(第)1, 2화(花)와 같은 정도의 화장(花長)과 수명(壽命)을 유지(維持)할 수 있었다. 4. 보존용액(保存溶液)에 sucrose와 $AgNO_3$, HQ가 모두 혼합(混合)될 때 화장(花長), 흡수량(吸收量), 생체중(生體中), 절화수명연장등(切花壽命延長生等)에 좋은 효과(效果)를 보였으며 sucrose만의 처리(處理)는 절화수명연장(切花壽命延長)에는 다소(多少) 효과적(效果的)이었으나 흡수량(吸水量)은 증류수(蒸留水)에 비(比)하여 감소(減少)되었고 생체중(生體重)과 화장(花長)의 증가(增加)에도 별 효과(效果)가 없었다.

• 복식
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• 제30권
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• pp.41-58
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• 1996

To purpose of this study is to understand the present situation of the present situation of the fashion careers in apparel industry and to present a activation information for development of a specialty de-partment. This study has been proceeded with the library survey method, and 20 apparel enterprises have been researched with the questionaire and inter-view method. These were large and medium sized enterprises. The results were as follows: 1. Subdivision of fashion careers have been found insufficient on apparel enterprises in Korea. 2. Textile specialists have been needed on apparel enterprises in Korea. 3. Domestic enterprises have dropt out man-agement 4. These enterprises are negative for the consumer research and fashion information col-lection. 5. Fashion sepecialists have been found insuf-ficient on retail division, The propositions for activation of fashion careers are as follows: 1. Fashion careers should subdivide and specialize. 2. The apparel enterprises should employ textile designer. 3. The apparel enterprises should activate practical department. 4. The apparel enterprises should research consumer and collect fashion information. 5. The apparel enterprises should develop fashion specialists on retail division.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1994년도 추계학술대회 논문집 학회본부
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• pp.196-198
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• 1994

The dielectric and electrical properties of $Pb(Mg_{1/3}Nb_{2\3})O_3-PbTiO_3-BaTiO_3$ have been investigated. Perovskite crystalline phase of the specimens are confirmed by XRD at $1270^{\circ}C$. The curie temperature is around $40^{\circ}C$ with the amount of $BaTiO_3$ and $PbTiO_3$. Every specimens shows the slim loop hysteresis curves which is electrostrictive characteristic. The strain vs. applied voltage characteristics exhibits nonlinear relationship, and the specimen of 0.85PMN-0.125PT-0.025BT shows the largest strain.