• 제목/요약/키워드: COL3A1

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Development of an E. coli Expression Cassette for the Efficient Production of a Harmful Protein

  • Kim Ok Soo;Kwak Hwan Jong;Lee Jae-Hwa;Ha Jong Myung;Ha Bae-Jin;Lee Sang-Hyeon
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.9 no.5
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    • pp.389-392
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    • 2004
  • In order to produce a harmful protein more efficiently, this expression cassette, dubbed pCol-MICT, is directed by the colicin promoter, and was constructed by the insertion of a $rrnBT_1T_2$ fragment of pEXP7, and a MxelnteinCBD fragment of pTXB3, into pSH375. To test whether harmful proteins, including proteolytic enzymes, could be effectively produced by this cassette, the carboxypeptidase (CPase) Taq gene was inserted into the pCol-MICT cassette to yield pCol-CPase Taq-MICT. E coli W3l 10 tells harboring pCol-CPase Taq-MICT produced a large quantity of this enzyme, as much as 47.2 mg of purified from per liter of culture, when cultured in the presence of mitomycin C ($0.4{\mu}g/mL$). This indicates that the colicin promoter-controlled E, coli expression cassette was able to produce almost 8 times of protein than the conventional tar promoter-based system, and that this cassette may be useful in the Synthesis of other harmful proteins.

A Composite Dermal Filler Comprising Cross-Linked Hyaluronic Acid and Human Collagen for Tissue Reconstruction

  • Kim, Z-Hun;Lee, Yongjun;Kim, Sun-Mi;Kim, Hojin;Yun, Chang-Koo;Choi, Yong-Soo
    • Journal of Microbiology and Biotechnology
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    • v.25 no.3
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    • pp.399-406
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    • 2015
  • In this study, we developed a composite filler comprising cross-linked hyaluronic acid (HA) and human collagen (COL) derived from the human umbilical cord with the aim of improving its biocompatibility and longevity compared with commercially available fillers. After HA/COL composite fillers were made in two different ratios (10:1 and 5:1), the physical properties of the fillers were evaluated. The interior morphologies and in vivo weight change of these hydrogels were also characterized at 1-16 weeks after injection into mice. To evaluate their biocompatibility and durability in vivo, we injected the composite fillers into nude mice subcutaneously. The variations of injected gel weight were measured and compared with the commercial dermal fillers (Restylane and TheraFill). The composites showed improved or similar physical properties (complex viscosity of 19-22 × 105 cP, and injection force of 10-12 N) over the commercial dermal fillers. Sixteen weeks following the injection, the ratio of remaining composite filler weight to initial weight (75.5 ± 16.9%; 10:1) was shown to be greater than that of the commercial fillers (43.2 ± 8.1%, Restylane; 12.3 ± 5.3%, TheraFill). In addition, immunohistochemical analysis with angiogenesis-related markers such as isolectin and vWF revealed newly formed blood vessels and cellular influx into the composite filler, which were not observed in the other fillers. These results clearly suggest that the HA/COL composite filler is a superior candidate for soft tissue reconstruction. The filler we developed may be a suitable candidate as an injectable dermal filler for tissue augmentation in humans.

Deposition and Properties of Pt/ST/Pt Thin Film Structure (Pt/ST/Pt 소자 구조의 박막증착 및 특성)

  • Kim, Jin-Sa;Cho, Choon-Nam;Oh, Yong-Cheul;Shin, Cheol-Gi;Song, Min-Jong;So, Byeong-Mun;Choi, Woon-Shick;Kim, Chung-Hyeok
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2007.06a
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    • pp.472-473
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    • 2007
  • The $(Sr_{1-x}Ca_x)TiO_3$(ST) thin films are deposited on Pt-coated electrode(Pt/TiN/$SiO_2$/Si) using RF sputtering method with substitutional contents of Ca. The maximum grain of thin films is obtained by substitution of Ca at 15[mol%]. Also, the composition of ST thin films were closed to stoichiometry(1.081~1.117 in A/B ratio). The dielectric constant changes almost linearly in temperature ranges of -80~+90[$^{\circ}C$]. The current-voltage characteristics of ST15 thin films showed the increasing leakage current as the measuring temperature increases.

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LATITUDINAL DISTRIBUTION OF SUNSPOTS AND DURATION OF SOLAR CYCLES

  • CHANG, HEON-YOUNG
    • Journal of The Korean Astronomical Society
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    • v.48 no.6
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    • pp.325-331
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    • 2015
  • We study an association between the duration of solar activity and characteristics of the latitude distribution of sunspots by means of center-of-latitude (COL) of sunspots observed during the period from 1878 to 2008 spanning solar cycles 12 to 23. We first calculate COL by taking the area-weighted mean latitude of sunspots for each calendar month to determine the latitudinal distribution of COL of sunspots appearing in the long and short cycles separately. The data set for the long solar cycles consists of the solar cycles 12, 13, 14, 20, and 23. The short solar cycles include the solar cycles 15, 16, 17, 18, 19, 21, and 22. We then fit a double Gaussian function to compare properties of the latitudinal distribution resulting from the two data sets. Our main findings are as follows: (1) The main component of the double Gaussian function does not show any significant change in the central position and in the full-width-at-half-maximum (FWHM), except in the amplitude. They are all centered at ~ 11° with FWHM of ~ 5°. (2) The secondary component of the double Gaussian function at higher latitudes seems to differ in that even though their width remains fixed at ~ 4°, their central position peaks at ~ 22.1° for the short cycles and at ~ 20.7° for the long cycles with quite small errors. (3) No significant correlation could be established between the duration of an individual cycle and the parameters of the double Gaussian. Finally, we conclude by briefly discussing the implications of these findings on the issue of the cycle 4 concerning a lost cycle.

A study on Dielectric and Electrical Properties Using PMN-PT-BT Ceramics (PMN-PT-BT계 세라믹스의 유전 및 전기적 특성)

  • Ji, S.H.;Lee, N.H.;Kim, Y.H.;Kim, J.S.;Cho, S.W.;Lee, D.C.
    • Proceedings of the KIEE Conference
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    • 1994.07b
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    • pp.1481-1483
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    • 1994
  • The dielectric and electrical properties of $Pb(Mg_{1/3}Nb_{2/3})O_3-PbTiO_3-BaTiO_3$ have been investigated. Perovskite crystalline phase of the specimens is confimed by XRD. The curie temperature decrease with increasing $BaTiO_3$ and hysteresis phenomena decrease with increasement of $BaTiO_3$. The strain vs. applied voltage characteristics exhibit nonlinear relationship and the specimen. of 0.9PMN-0.08PT-0.02BT shows the largest strain.

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The Paleoenvironment of the Northern Part of the Nagdong River Delta (낙동강 삼각주 북부의 고환경)

  • 오건환
    • The Korean Journal of Quaternary Research
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    • v.8 no.1
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    • pp.33-42
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    • 1994
  • The study area is filled up by alluvium which can be classified into lower and upper beds by the facies of deposits and their degree of weathering. The lower bed is not of marine but of fluvial. The upper bed is mainly composed of clay and silt or fine sand beds col-ored dark or dark gray which are of neritic or brackish yielding mollusca fossils. The oyster(Crassostrea ariakensis) obstained from the depth of 3m below the present sea level in the upper bed was dated at 4,100 110Y.B.P. and the veneridae(Meretrix lamarck) 1m height above the present delta plain in the old sand bar was dated at 1,700 85Y,B,P. It is therfore concluded that the study area a small kern col of terrestri-al basin prior to postglacial age had experienced the Holocene transgression. Consequently the study area is considered to have been progressively formed under neritic or brackish circumstance before 1,700Y.B.P.

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An epidemiologic study on the cleft lip and/or palate patients who visited Dept. of Orthodontics, Pusan National University Hospital (부산대학교병원 교정과에 내원한 구순구개열 환자들에 대한 역학조사)

  • Son Woo-Sung;Baek Jae-Ho;Lee Won-Chul
    • Korean Journal of Cleft Lip And Palate
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    • v.5 no.1
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    • pp.43-58
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    • 2002
  • A General survey on the cleft lip and/or palate patients in Pusan National University Hospital was done. The conclusions were as follow. 1. In gender ratio, male was higher than female. 2. In the cleft type, unilateral cleft lip and palate, cleft lip and alveolus, and bilateral cleft lip and palate in higher ratio order. Left unilateral cleft lip and palate was higher than right in ratio. 3. In first hospital visiting age for dental treatment, the group from 6 years old to 12 years old was most and in that the age group for attending elementary school (about 7-8 years old) showed especially higher ratio. The large majority of patient were born in 1980's and lived in Pusan metropolitan city, KyungSangNamDo. Especially, most of them lived in the neighboring Held of Pusan National University Hospital. 4. Class III skeletal pattern and Angle's Class III molar relationship was most prevalent. Angle's Class II molar relationship showed relative higher ratio because tooth loss and malposition. 5. Primary lip closure in 3 months, secondary lip closure in 6-7 years old, palatal closure in 12-16 months and bone graft in 8-10 years old were operated mostly. Rhinoplasty and scar revision were operated in too early age so the need of infantile orthopedics must be considered. 6. Maxillary expansion and protraction were operated mostly in 8-11 years old but operating in primary dentition must be considered. 7. Tooth alignment were started mostly in mixed dentition and consideration about prosthodontic treatment and retention will be need. 8. In tooth anomaly, tooth malformation and missing were most prevalent.

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Effects of Ipriflavone on bone remodeling in the rat calvarial cell (백서 두개관세포에서 Ipriflavone이 골조직 개조에 미치는 영향)

  • Lee, Yong-Seung;Kim, Young-Jun;Lee, Ki-Heon;Hwang, Hyeon-Shik
    • The korean journal of orthodontics
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    • v.35 no.4 s.111
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    • pp.275-285
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    • 2005
  • Ipriflavone (isoprofoxyisoflavone), a synthetic derivative from soy isoflavone diazein, has been shown to inhibit bone resorption and perhaps stimulate bone formation This study was performed to examine the effects of ipriflavone on the proliferation and bone remodeling in rat calvarial cells in vitro The rat calvarial cells were isolated from fetus aged 20 to 21 days and cultured In BGJb media The graded concentration of ipriflavone $(10^{-9}\;10^{-5}M)$ was administered into cultured cells. When the cell proliferation was estimated through the measurement of MTT assay, there was no increase in cellular proliferation of the rat calvarial cell at any ipriflavone concentration. The cellular activity was evaluated through the formation of mineralized nodules stained by alizarin red. The formation of mineralized nodules significantly increased at concentrations of $10^{-8}M,\;10^{-7}M\;and\;10^{-6}M$ ipriflavone. Reverse transcription-polymerase chain reaction analyses (RT-PCR) were done at 7 and 14 days after culture to detect the expression of Bone Sialoprotein (BSP), Type I Collagen (COL I) and Osteocalcin(OCN) As a result, the expressions of BSP and COL I increased on the 7th day of culture and the expression of OCN increased on the 14th day of culture. These results indicate that ipriflavone facilitates the bone remodeling process bvy promoting rat calvarial cell differentiation aid stimulating mineralization through increased expression of extracellular matrix genes. such as BSP. COL I and OCN.

A Case of Platyspondylic Lethal Skeletal Dysplasia Torrance Type

  • Lee, Sung Chan;Choi, Min Seon
    • Neonatal Medicine
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    • v.25 no.1
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    • pp.44-48
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    • 2018
  • Platyspondylic lethal skeletal dysplasia, Torrance type (PLSD-T), is one of the pheno-types of type II collagenopathy and is characteristic of severe bone growth disorder. This phenotype may limit the growth and expansion of the lungs, which is known to cause death from respiratory failure during or shortly after birth, but in few less severe cases, patients have been reported to have survived to adulthood. We have experienced a case of PLSD-T in a preterm infant who was delivered via cesarean section at the gestational age of 29 weeks 3 days, with a birth weight of 1.15 kg. Physical examination of the infant revealed characteristic findings of short arms and legs, small thorax, distended abdomen, and cleft palate. On the basis of the subsequent genetic testing, the patient had a heterozygous mutation in the encoded c-propeptide region of collagen, type II, alpha 1 (COL2A1), c.4335G>A ($p.Trp1445^{\ast}$) in exon 52. This is the first case of PLSD-T diagnosed in Korea, and we hereby report the case.