• Journal of Physiology & Pathology in Korean Medicine
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• v.36 no.2
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• pp.79-87
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• 2022

In this study, extracts of Boswellia serrata gum resin, Curcuma longa rhizome, and Terminalia chebula fruit were combined in different ratios, and their anti-osteoarthritis effects were compared to determine which combination had the best synergistic effect. B. serrata, C. longa, and T. chebula extracts in a 2:1:2 ratio exhibited higher antioxidative activity in scavenging DPPH radicals than did the individual extracts alone or the other extract combinations. Additionally, the 2:1:2 combination significantly improved the levels of enzymatic antioxidants and antioxidant-related proteins. Moreover, this same combination ratio decreased the protein levels of matrix metalloproteinase (MMP) 3 and MMP13 in interleukin-1β-stimulated human articular chondrocytes (HCHs) and increased those of aggrecan and collagen type II alpha 1 chain (COL2A1). Analysis of the underlying mechanisms revealed that the 2:1:2 combination significantly inhibited the phosphorylation of nuclear factor kappa B (NF-κB) p65, extracellular regulated protein kinase (ERK), and p38 mitogen-activated protein kinase (MAPK). Therefore, the 2:1:2 combination of these three plant extracts has the best potential for use as an effective dietary supplement for improving joint health compared with the individual extracts and their other combination ratios.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.50 no.2
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• pp.171-178
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• 2024

In this study, we aimed to determine the various effects of Osmanthus fragrans (O. fragrans) flower extract on the skin in order to utilize it as a cosmetic material. For this purpose, Osmanthus fragrans flower extract (OFFE) of Jeju Island was prepared and used in the experiment. The experiments were evaluated by the quantitative real-time polymerase chain reaction (qRT-PCR) and lipid droplet staining assay. First, the OFFE decreased the gene expressions of three representative pro-inflammatory cytokines (IL-8, IL-6, and IL-1α) and an inflammation-related enzyme, PTGS2 induced by poly I:C in epidermal keratinocytes. In addition, the OFFE increased the gene expression levels of collagen (COL1A1) and elastin (ELN) in dermal fibroblasts. Further, the OFFE showed the inhibitory effect in sebum production by linoleic acid in sebocytes. Therefore, from this study, it is expected that OFFE can be used as a natural cosmetic material for anti-inflammatory, anti-aging, and sebum inhibitory efficacy.

• Proceedings of the KIEE Conference
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• 1987.11a
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• pp.499-503
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• 1987

The electrical characteristics of Epoxy Robin vary with temperature. In this study, the variation of the electric characteristics of Epoxy Resin with various hardening temperature was studied. Epoxy Resin used as samples are Epotohto YD-128 of Diglycidyl Ether of Bisphenol A (DGEBA) series and hardener, Goodmide G-240. It's mixing rate is 3 to 1. Hardening temperature was varied from 25[$^{\circ}C$] to 50[$^{\circ}C$] by 5[$^{\circ}C$]. During the hardening progress, illumination, resistivity and the internal temperature of material which were dependet on the variety of hardening temperature were measured. The electrical properties of hardened samples were examined by means of X-ray diffraction, tan $\delta$ test, TG-DTA analysis and treeing test. On this basis, optimal hardening temperature in insulation characteristics was examined.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1996.04a
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• pp.188-188
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• 1996

In order to gain insight into the mechanism of the regulation of cytochrome P450IAl by arylhydrocarbon, the 5'-flanking region of a trout CYP450IAl 5'flanking DNA was cloned into pCAT-basic vector and it was transfected into Hepa-1 cells. 3MC treatment to hepa Ⅰ cells transfected with fish CYP450IAl-CAT construct results in mRNA increased by 2.81 fold when it was compared with that of control This increase of mRNA was decreased by concomitantly treated flavonoids such as morin. The levels of CAT mRNA that was treated with morin was 29.2-58.0% of 3MC stimulated CAT mRNA. Further investigation to find out if there are DRE, XRE or negative regulatory cis element in CYP450IA1 gene was undertaken. Results of the deletion study of 5'flanking DNA of trout P450IA indicate the existance of the negative(-1600 ～ -1300). CAT mRNA was about two-fold higher in deleted trout CYP450IAl-CAT construct transfected cells compared to the wi Id type trout CYP450IAl-CAT construct transfected cells. And The stimulatory effect of 3MC was no longer observed in col Is containing deleted CAT construct. [Supported by grants from the Korean Ministry of Education]

• International Journal of Oral Biology
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• v.39 no.3
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• pp.145-151
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• 2014

During bone remodeling, there is requirement of differentiation of osteoblastic cells. Previously, we identified proteins differentially expressed in soft tissue during bone healing. Of these proteins, we focused the effect of LTF on differentiation of osteoblast. In order to analyze the osteogenic ability of LTF, we treated conditioned media collected from human LTF-stably transfected HEK293T cells into osteoblastic MC3T3-E1. The results showed that the activity and expression of alkaline phosphatase were increased in MC3T3-E1 cells treated with conditioned media containing LTF in dose- and time-dependent manner. At the same time, we observed the significant increase of the expression of osteoblastic genes, such as ALP, BSP, COL1A1, and OCN, and along with matrix mineralization genes, such as DMP1 and DMP2, in LTF conditioned media-treated groups. Moreover, the result of treating recombinant human LTF directly into osteoblastic MC3T3-E1 showed the same pattern of treating conditioned media containing LTF. Our study demonstrated that LTF constitutively enhances osteoblastic differentiation via induction of osteoblastic genes and activation of matrix mineralization in MC3T3-E1 cells.

• The Journal of Advanced Prosthodontics
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• v.6 no.3
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• pp.157-164
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• 2014

PURPOSE. This study focused on in vitro cell differentiation and surface characteristics in a magnesium coated titanium surface implanted on using a plasma ion source. MATERIALS AND METHODS. 40 commercially made pure titanium discs were prepared to produce Ti oxide machined surface (M) and Mg-incorporated Ti oxide machined surface (MM). Surface properties were analyzed using a scanning electron microscopy (SEM). On each surface, alkaline phosphatase (ALP) activity, alizarin red S staining for mineralization of MC3T3-E1 cells, and quantitative analysis of osteoblastic gene expression, were evaluated. Actin ring formation assay and gene expression analysis of TRAP and GAPDH performing RT-PCR were performed to characterize osteoclast differentiation on mouse bone marrow-derived macrophages (BMMs). RESULTS. MM showed similar surface morphology and surface roughness with M, but was slightly smoother after ion implantation at the micron scale. M was more hydrophobic than MM. No significant difference between surfaces on ALP activity at 7 and 14 days were observed. Real-time PCR analyses showed similar levels of mRNA expression of the osteoblast phenotype genes; osteopontin (OPN), osteocalcin (OCN), bone sialoprotein (BSP), and collagen 1 (Col 1) in cell grown on MM at 7, 14 and 21 days. Alizarin red S staining at 21 days showed no significant difference. BMMs differentiation increased in M and MM. Actin ring formation assay and gene expression analysis of TRAP showed osteoclast differentiation to be more active on MM. CONCLUSION. Both M and MM have a good effect on osteoblastic cell differentiation, but MM may speed the bone remodeling process by activating on osteoclast differentiation.

• Progress in Superconductivity and Cryogenics
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• v.3 no.1
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• pp.40-44
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• 2001

Recently. the high-tc superconducting fault col-rent limiters (SFCL) are studied worldwide to be classified as a resistive type or an inductive type such as a magnetic shielding type and a inductive type. The high-tc SFCL wish an open core belongs to the magnetic shielding type SFCL. Unlike conventional magnetic shielding type SFCLS it uses the open core to reduce the mechanical vibrations and installation space, The high-tc SFCL with an open core was designed and manufactured by stacking three BSCCO 2212 tubes. It was tested in the maximum source voltage of 400 Vrms. The results such as the reduction of fault current and impedance of the SFCL are described in this paper. The results show that the fault current in the source voltage of 400 Vrms was reduced to be about 123 Apeak. about 3.9 times greater than the normal state current. Also, the impedance of the high-tc SFCL was about 9${\Omega}$ about 9 times greater than the normal state impedance. The impedance of the SFCL appears just after the fault, and its size is dependent on the source voltage. From the impedance, the inductance of the SFCL was calculated.

• Research in Plant Disease
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• v.16 no.3
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• pp.312-315
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• 2010

Pectobacterium carotovorum subsp. carotovorum (Pcc) causes soft rot disease in various plants. Although many studies about Pcc have been going on, little is known yet about the defense genes from plants. To identify defense associated genes in response to Pcc, we screened about 20 thousand Arabidopsis T-DNA knock out lines by inoculation with Pcc. We obtained a line (Atspt1) showing more susceptible symptom compared to WT (Col-0) on 1 day after the inoculation of Pcc on leaves of Arabidopsis with toothpicks. In this study, we optimized the system to select resistant and susceptible lines to Pcc from T-DNA inserted pool of Arabidopsis and expect the system and Atspt1 might be used for molecular breeding to produce resistant vegetables against Pcc.

• Proceedings of the Korea Information Processing Society Conference
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• 2014.04a
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• pp.819-822
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• 2014

본 연구에서는 비디오 감시 시스템을 기반으로 한우 축사에서 야간 승가 행위 검출을 위한 최적의 방법을 제안한다. 특히 축사 환경에서는 소들간의 겹침 등 다양한 어려움이 발생하기 때문에, 이를 극복하기 위하여 깊이 정보를 이용하여 승가하는 소의 머리를 자동으로 탐지한다. 즉, 소가 네발로 걸어다니는 통상의 경우 소의 등 높이가 1.3m 정도인데 반해 앞발을 들어 승가하는 경우에는 소의 높이가 1.7m 까지 높아짐에 착안하여, 축사 측면에 설치된 깊이 카메라로부터 소까지의 거리 차이를 이용하면 발정기 탐지를 위한 승가 행위를 자동으로 검출할 수 있음을 확인하였다.

• BMB Reports
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• v.34 no.6
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• pp.584-589
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• 2001

One of the cryptic plasmids from the oil degrading bacterium Klebsiella sp. KCL-2, the small plasmid pGD2, has been identified and characterized. This plasmid has a size of 3.6 kb with unknown functions. We constructed the recombinant plasmid pMGD2. The nucleotide sequences of the plasmid were determined and two open reading frames were detected. ORF1 encodes a replication initiator protein (RepA), which has a high degree of homology with the protein of ColE2 plasmid. The product encoded by ORF2 showed a high similarity with the transposase protein of IS5. IS5 is 1195 by long and contains an inverted terminal repetition of 16 bp with one mismatch. Stem-loop structures in the 5'untranslated region of the repA suggest that a putative gene, incA, is located in a complementary strand to the leader region of the repA mRNA.