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http://dx.doi.org/10.5423/RPD.2010.16.3.312

Selection of a Susceptible Line (Susceptible to Pectobacterium 1, Atstp1) to Soft-rot Disease in T-DNA Insertion Mutants Pool of Arabidopsis  

Choi, Chang-Hyun (National Academy of Agricultural Science, RDA)
Kim, Min-Gab (National Academy of Agricultural Science, RDA)
Ahn, Il-Pyung (National Academy of Agricultural Science, RDA)
Park, Sang-Ryeol (National Academy of Agricultural Science, RDA)
Bae, Shin-Chul (National Academy of Agricultural Science, RDA)
Hwang, Duk-Ju (National Academy of Agricultural Science, RDA)
Publication Information
Research in Plant Disease / v.16, no.3, 2010 , pp. 312-315 More about this Journal
Abstract
Pectobacterium carotovorum subsp. carotovorum (Pcc) causes soft rot disease in various plants. Although many studies about Pcc have been going on, little is known yet about the defense genes from plants. To identify defense associated genes in response to Pcc, we screened about 20 thousand Arabidopsis T-DNA knock out lines by inoculation with Pcc. We obtained a line (Atspt1) showing more susceptible symptom compared to WT (Col-0) on 1 day after the inoculation of Pcc on leaves of Arabidopsis with toothpicks. In this study, we optimized the system to select resistant and susceptible lines to Pcc from T-DNA inserted pool of Arabidopsis and expect the system and Atspt1 might be used for molecular breeding to produce resistant vegetables against Pcc.
Keywords
Arabidopsis; Pcc; Pectobacterium; Soft rot; T-DNA knock out lines;
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Times Cited By KSCI : 2  (Citation Analysis)
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