• 제목/요약/키워드: COL

검색결과 578건 처리시간 0.029초

The complete mitochondrial genome of Arabidopsis thaliana (Brassicaceae) isolated in Korea

  • PARK, Jongsun;XI, Hong;KIM, Yongsung
    • 식물분류학회지
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    • 제51권2호
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    • pp.176-180
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    • 2021
  • Arabidopsis thaliana (L.) Heynh. is a small plant species that serves as a model organism of plant biology and genetics. Here, we present the first complete mitochondrial genome of Korean A. thaliana natural isolate (named as 180404IB4), which is 368,875 bp long and contains 58 genes (33 protein-coding genes, 22 tRNAs, and three rRNAs), with a GC ratio of 44.8%. Sixty-four single-nucleotide polymorphisms and 11 insertion and deletion regions (1,089 bp in length) are identified against the Col-0 ecotype, showing one large insertion of 1,069 bp without structural variation. Phylogenetic trees constructed from 30 conserved genes indicate that the 180404IB4 mitochondrial genome is clustered with Col-0 and three East Asian ecotypes.

제주도의 소귀나무 자생지 조사, 실생 및 삽목번식 (Native Habitat Survey of Wax Myrtle in Cheju Province and Its Propagation by Seed and Cutting)

  • 고성준;강훈;지성한;장전익
    • 생물환경조절학회지
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    • 제6권3호
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    • pp.225-234
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    • 1997
  • 제주지방의 소귀나무 자생지 분포조사와 방법을 구명하기 위한 삽목 번식 및 종자 번식에 대한 시험결과를 요약하면 다음과 같다. 1. 자생지 분포는 서귀포시 영천동과 남제주군 남원읍 하례 2리를 경계하고 있는 효돈천 및 동홍천을 중심으로 해발 100~400m에 집중적으로 분포되어 있었다. 2. 삽목에서 발근을 촉진하기 위하여 BA, IBA, Kinetin, IAA, GA$_3$ 등을 처리하였으나 발근 발아가 되지 않았다. 3. 발근저해물질인 탄닌 성분을 제거하기 위해 AgNO$_3$를 처리한 다음 BA, lBA, Kinetin 처리에서도 역시 발근은 되지 않았다. 4. 종자를 4$0^{\circ}C$에서 30일간 처리한 것이 39%의 발아율을 나타냈다. 5. 소귀나무 어린묘의 잎의 형태와 성목의 잎의 형태가 완전히 다르게 전개되는 특성을 보였다.

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부산대학교병원 교정과에 내원한 구순구개열 환자들에 대한 역학조사 (An epidemiologic study on the cleft lip and/or palate patients who visited Dept. of Orthodontics, Pusan National University Hospital)

  • 손우성;백재호;이원철
    • 대한구순구개열학회지
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    • 제5권1호
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    • pp.43-58
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    • 2002
  • A General survey on the cleft lip and/or palate patients in Pusan National University Hospital was done. The conclusions were as follow. 1. In gender ratio, male was higher than female. 2. In the cleft type, unilateral cleft lip and palate, cleft lip and alveolus, and bilateral cleft lip and palate in higher ratio order. Left unilateral cleft lip and palate was higher than right in ratio. 3. In first hospital visiting age for dental treatment, the group from 6 years old to 12 years old was most and in that the age group for attending elementary school (about 7-8 years old) showed especially higher ratio. The large majority of patient were born in 1980's and lived in Pusan metropolitan city, KyungSangNamDo. Especially, most of them lived in the neighboring Held of Pusan National University Hospital. 4. Class III skeletal pattern and Angle's Class III molar relationship was most prevalent. Angle's Class II molar relationship showed relative higher ratio because tooth loss and malposition. 5. Primary lip closure in 3 months, secondary lip closure in 6-7 years old, palatal closure in 12-16 months and bone graft in 8-10 years old were operated mostly. Rhinoplasty and scar revision were operated in too early age so the need of infantile orthopedics must be considered. 6. Maxillary expansion and protraction were operated mostly in 8-11 years old but operating in primary dentition must be considered. 7. Tooth alignment were started mostly in mixed dentition and consideration about prosthodontic treatment and retention will be need. 8. In tooth anomaly, tooth malformation and missing were most prevalent.

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Production of ColE1 Type Plasmid by Escherichia coli $DH5\alpha$ Cultured Under Nonselective Conditions

  • PASSARINHA L. A.;DIOGO M. M.;QUEIROZ J. A.;MONTEIRO G. A.;FONSECA L. P.;PRAZERES D. M. F.
    • Journal of Microbiology and Biotechnology
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    • 제16권1호
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    • pp.20-24
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    • 2006
  • Plasmid DNA (pDNA) is a product of interest for many biopharmaceutical companies and research laboratories, because of increase in the number of gene therapy protocols that use nonviral vectors. This work was undertaken to study the effect of antibiotic and dissolved oxygen concentration (DOC) on the production of a ColE 1-type plasmid (pVAX1-LacZ) hosted in Escherichia coli $DH5\alpha$ and cultured in a batch fermentor with 0.751 of Terrific Broth. A decrease in the DOC from $60\%\;to\;5\%$ was shown to increase the specific pDNA concentration approximately 1.5-fold, due to the downregulation of growth. Additionally, this increase in the pDNA concentration led to a 2.2-fold increase in the purity of cell lysates obtained after cell lysis. However, the use of higher DOC led to 2.8-fold higher volumetric productivity as a consequence of a faster growth rate, reducing the fermentation time from 24 to 8 h. Interestingly, the specific pDNA concentration, and pDNA productivity and purity were always higher $(10-15\%)$ in the absence of antibiotic. Overall, the data indicate that nonselective conditions can be used without compromising yield, productivity, and purity of pDNA.