• 제목/요약/키워드: CO-I

검색결과 5,539건 처리시간 0.036초

Quantitation of Plasma Apolipoprotein A-I with a Sandwich Type Enzyme-Linked Immunosorbent Assay Using Monoclonal Antibodies

  • Lee, Min-Gyu;Kang, Jae-Seon;Jeong, Jae-Yeon;Jue, Dae-Myung;Kim, Hack-Joo
    • BMB Reports
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    • 제30권6호
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    • pp.390-396
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    • 1997
  • A sandwich-type enzyme-linked immunosorbent assay (ELISA) for the quantification of human apolipoprotein A-I (apoA-I) was developed using monoclonal antibodies. For this assay, we used three monoclonal antibodies to trap and detect apo A-I. HDAI16 and HDA15 monoclonal antibodies were used for trapping apoA-I and HDAI8 monoclonal antibody was for detecting apoA-I. These three monoclonal antibodies were produced by immunizing mice with high density lipoprotein (HDL) isolated from human plasma. By immunoblot analysis, these three monoclonal antibodies were specific to apoA-I and showed no cross-reactivities with other plasma proteins. The results of competition assays for epitope cross-reactivity test also verified that these monoclonal antibodies identified separate and distinct epitopes on HDL and apoA-I. Affinity constants of monoclonal antibodies were measured by ELISA. Their association constants ranged from $10^7$ to $10^8$ $M^{-1}$. For this assay, pure apoA-I was isolated by affinity chromatography using monoclonal antibodies. In this sandwich assay, the amount of HRP-labeled HDAI8 bound to apoA-I trapped by HDAI16 and HDAI5 was proportional to apoA-I concentration in the range of 0 to 500ng/ml. ApoA-I concentration in plasma was calculated from the linear regression equation of standard curve. The precision and reliability of the assays are reflected in the low intra-and interassay coefficients of variation that averaged 3.25% and 4.30%, respectively. This assay is sensitive, simple, reproducible, convenient in incubation interval, and does not use radioisotope: thus it can be widely applied in clinical laboratories.

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탄산가스 소비율을 고려한 $CO_2$농도 제어알고리즘 개발(I) (Development of $CO_2$ concentration control algorithm considering $CO_2$ consumption rate(I))

  • 진제용;홍순호;류관희;노상하
    • 한국생물환경조절학회:학술대회논문집
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    • 한국생물환경조절학회 1992년도 학술논문발표요지
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    • pp.14-14
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    • 1992
  • 시설원예에 있어서 작물의 생장량을 촉진시켜 수확시기를 앞당기고, 생산량을 증가시키며, 품질을 향상시키기 위하여 탄산가스를 시비하는 재배방법이 도입되고 있다. 그러나 탄산가스의 시비가 작물에 악영향을 주는 경우도 보고되고있어 탄산가스 시비에 주의를 기울여야 한다. 기존의 탄산가스 시비방법은 일정한 농도를 유지하는 것으로 탄산가스 낭비나 고농도에 의한 생육장애 혹은 탄산가스 결핍을 초래하는 등의 문제점을 갖고 있다. (중략)

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광합성의 생리생태(1) - 광도와 엽육내 $CO_2$분압 변화에 대한 광합성 반응 - (Ecophysiology of Photosynthesis 1: Effects of Light Intensity and Intercellular $CO_2$ Pressure on Photosynthesis)

  • 김판기;이은주
    • 한국농림기상학회지
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    • 제3권2호
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    • pp.126-133
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    • 2001
  • 녹색식물과 같이 독립영양생물이 빛에너지를 이용하여 $CO_2$와 물로 탄수화물을 만드는 일련의 반응을 광합성이라 한다. 그 일련의 반응 중에서(그림 1) 반응 I과 II는 틸라코이드(thylakoid)에서, 반응 III은 스트로마(stroma)에서, 반응 IV는 엽록체내와 세포질에서 일어나는 반응의 상호작용에 의하여 나타난다. 과거에는 반응 I과 II를 명반응, 반응 III과 IV를 암반응이라 부르기도 하였다.(중략)

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이종(異種)의 전투기동차량 간 전술정보 연동 방안 검토 (A Research on Intercommunication Method for Tactical Information Among Heterogeneous Mobile Combat Vehicles)

  • 최일호;노해환;송충호
    • 한국군사과학기술학회지
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    • 제25권2호
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    • pp.187-195
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    • 2022
  • Modern Battle Fields are covered with various weapon systems, such as tanks, infantry vehicles, or artillery vehilces. In such environment, it is important to take it into consideration what kind of efficient method should be developed to communicate with heterogeneous friend combat systems for the exchange of tactical information aqcuired because military operations require coordinated actions. For this purpose, we made a research on tactical information equipments such as Battlefield Management Systems(BMSs) equipped in multiple kinds of Mobile Combat Vehicles(MCVs). The exchange of tactical information could be divieded into separate domains, such as connectivity, messages, and recognition processes by operators. In this article, we will deal with wireless radio connectivity, KVMF messages, and User Interfaces showing shared data.

$H_2-CO_2$에서 $FeAl_2O_4$의 생성기구와 $CO_2$ 중에서의 거동(I) (Formation of $FeAl_2O_4$ in $H_2-CO_2$ and its behavior in $CO_2$(I))

  • 이홍림;강명구
    • 한국세라믹학회지
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    • 제19권4호
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    • pp.309-315
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    • 1982
  • $FeAl_2O_4$ was formed from the starting material of $Fe_2O_3$ and $Al_2O_3$ by controlling the oxygen partial pressure using $H_2-CO_2$ gas mixture, over the temperature range of 800~120$0^{\circ}C$. The formation mechanism of $FeAl_2O_4$ was found to be a second order chemical reaction, and the activation energy of formation was observed as 39.97 kcal/mole. Vaporization behavior of $FeAl_2O_4$ under $CO_2$ atmosphere was observed over the temperature range of 800~120$0^{\circ}C$. $FeAl_2O_4$ was vaporized by a second order chemical reaction and the activation energy was found to be 21.8kcal/mole. Electrical conductivity of $FeAl_2O_4$ was also measured.

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AI-Diecasting 공법을 적용한 경전철용 AC 견인전동기 개발 (The Development of AC Traction Motor by AI-Diecasting Process for LRV)

  • 이정일;박정태;이종인;권중록;우종열;조봉환
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 2000년도 하계학술대회 논문집 B
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    • pp.1006-1008
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    • 2000
  • In this material, a traction motor for light railway vehicle was developed. Aluminium -Diecasting process was selected in order to reduce the manufacturing cost of making rotor bars and rotor rings of the motor, instead of copper or copper alloy rotor bars and rotor rings. And the results of experiment satisfied the international standard IEC 349-2.

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