• Title/Summary/Keyword: CL/P

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Modulation of the Specific Interaction of Cardiolipin with Cytochrome c by Zwitterionic Phospholipids in Binary Mixed Bilayers: A $^2H$-and $^{31}P$-NMR Study

  • Kim, Andre;Jeong, In-Chul;Shim, Yoon-Bo;Kang, Shin-Won;Park, Jang-Su
    • BMB Reports
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    • v.34 no.5
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    • pp.446-451
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    • 2001
  • The interaction of cytochrome c with binary phospholipid mixtures was investigated by solid-state $^2H$- and $^{31}P$-NMR. To examine the effect of the interaction on the glycerol backbones, the glycerol moieties of phosphatidylcholine (PC), and cardioliph (CL) were specifically deuterated. On the binding of cytochrome c to the binary mixed bilayers, no changes in the quadrupole splittings of each of the components were observed for the PC/PG, PE/CL and PE/PG liposomes. In contrast, the splittings of CL decreased on binging of protein to the PC/CL liposomes, although those of PC did not change at all. This showed that cytochrome c specifically interacts with CL in PC/CL bilayers, and penetrates into the lipid bilayer to some extent so as to perturb the dynamic structure of the glycerol backbone. This is distinctly different from the mode of interaction of cytochrome c with other binary mixed bilayers. In the $^{31}P$-NMR spectra, line broadening and a decrease of the chemical shift anisotropy were observed on the binding of cytochrome c for all binary mixed bilayers that were examined. These changes were more significant for the PC/CL bilayers. Furthermore, the line broadening is more significant for PC than for CL in PC/CL bilayers. Therefore, it can be concluded that with the polar head groups, not only CL but also PC are involved in the interaction with cytochrome c.

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Electrical Properties of SBT Thin Films after Etching in Cl$_2$/Ar Inductively Coupled Plasma (Ar/Cl$_2$ 유도결합플라츠마 식각 후 SBT 박막의 전기적 특성)

  • 이철인;권동표;깅창일
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2002.07a
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    • pp.58-61
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    • 2002
  • SBT thin films were etched at different content of Cl$_2$in Cl$_2$/Ar plasma. We obtained the maximum etch rate of 883 ${\AA}$/min at Cl$_2$(20%)/Ar(80%). As Cl$_2$ gas increased in Cl$_2$/Ar plasma, the etch rate decreased. The maximum etch rate may be explained by variation of volume density for Cl atoms and by the concurrence of two etching mechanisms such as physical sputtering and chemical reaction with formation of low-volatile products, which can be desorbed only by ion bombardment. The variation of volume density for Cl, F and Ar atoms and ion current density were measured by the optical emission spectroscopy and Langmuir probe. To evaluate the physical damage due to plasma, X-ray diffraction and atomic force microscopy analysis carried out. After etching process, P-E hysteresis loops were measured by ferroelectric workstation.

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Reactive Ion Etching of InP, InGaAs and InAIAs by SiCl$_4$ and Cl$_2$ Gases: Effects of Gas Flow Rate, rf Power, Process Pressure and Ar Addition (SiCl$_4$와 Cl$_2$가스에 의한 InP, InGaAs 및 InAIAs의 반응성 이온 식각: 가스유량, rf 전력, 공정압력, Ar 첨가의 영향)

  • 유재수;송진동;배성주;정지훈;이용탁
    • Proceedings of the IEEK Conference
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    • 2001.06b
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    • pp.25-28
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    • 2001
  • In this paper, we have investigated the effects of gas flow rate, rf power, process pressure and Ar addition on reactive ion etching of InP, InGaAs and InAlAs using Sic14 and Cl$_2$ gases. The etch rates were measured by using a surface profiler. The etched profiles, sidewall roughness, and surface morphology were observed by scanning electron microscopy and by atomic force microscopy. The selective etching of InGaAs to InP and InAlAs was studied by varying the etching parameters. It was found that Cl$_2$ gas is more efficient for the selective etching of InGaAs to InAlAs than SiCl$_4$ gas. The etch selectivity of InGaAs to InAlAs is strongly dependent on the rf power and the process pressure.

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Effect of NaCl Concentration on the Quality of Kimchi during Fermentation (염농도에 따른 김치의 숙성중 품질특성 변화)

  • 박민경
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.3 no.1
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    • pp.27-31
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    • 2002
  • To estimate the effect of NaCl concentration in brine solution on quality of kimchi, chinese cabbage was brined At 5%, 8%, 10% or 15% NaCl. Titratable acidity, pH, mechanical texture and sensory quality of kimchi were measured during fermentation at 10'C. The salinity of kimchi brined at 15% NaCl was 2.49%, and the rates of pH decrease, titratable acidity acidity increase and mechanical texture decrease during fermentation were lower than those of kimchi brined at 5∼10% NaCl. Furthermore, sensory characteristics such as crispness, salty taste, sourness and overall quality of kimchi brined at 15% NaCl were in good estimation.

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Distribution of the Surface Charges of the Peats in Different Ionic Strengths (이온 강도(强度)가 다른 용액내(溶液內)에서 이탄표면(泥炭表面)의 하전특성(荷電特性))

  • Chang, Sang-Moon;Choi, Jyung
    • Applied Biological Chemistry
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    • v.30 no.1
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    • pp.1-8
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    • 1987
  • The distribution of the surface charges in two kinds of peats and their physico-chemical properties have a been studied by means of the potentiometric titration and by ion adsorption in different strength of electrolytes. The organic matter contents were 43.3% and 53.7% in Yeong Yang peat and Peong Tack peat, respectively. Their X-ray diffractograms showed that quartz was dominant in both peats, and illite, kaolinite, hydrated-halloysite and feldspars were contained in a trace. Total acidic group contents was 1.257me/g in Poeng Tack peat that was a little more than of Peong Yang heat were pKa values of Yeong Yang and Poeng Tack peat were 13.1 and 12.65, respectively. The titration curves at different ionic strength of electrolytes crossed at pH 3.9 and 4.4 in Yeong Yang peat, and pH 3.8 and 4.0 in Peong Tack peat. The pH ranges of suspensions when the net surfacecharge of the peats varied from positive to negative value were $pH\;4.55{\sim}5.20\;(NaCl)$ and $pH\;3.95{\sim}5.70\;(CaCl_2)$ in Yeong Fang peat, and $pH\;4.15{\sim}5.40\;(NaCl)$ and $pH\;3.80{\sim}4.15\;(CaCl_2)$ in Peong Tack peat. Therefore it is apparent that the zero point of charge of these peats was about pH 4.0.

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Dependence of 0.01M CaCl2 Soluble Phosphorus on Extractable P and P Sorptivity in Upland Soil (밭토양(土壤)에서 유효린산함량(有效燐酸含量)과 인산흡수능(燐酸吸收能)에 따른 0.01M CaCl2 가용(可溶) 인산농도(燐酸濃度) 변화(變化))

  • Yoon, Jung-Hui;Jung, Beung-Gan;Kim, Yoo-Hak
    • Korean Journal of Soil Science and Fertilizer
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    • v.31 no.3
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    • pp.266-270
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    • 1998
  • The identification of soil P level that exceed crop requirement is a prerequisite in implementing sustainable management of fertilizer and manure P to prevent soil and freshwater from contamination. To investigate the relationship between 0.01M $CaCl_2$ soluble P, and available P and P sorption capacity of 40 soils, P content and P sorptivity were analyzed. Single linear relationship revealed the dependence of 0.01M $CaCl_2-P$ on available P($r^2=0.479$), bioavailable P($r^2=0.281$), P sorption($r^2=-0.465$) and P absorption coefficient($r^2=-0.056^{NS}$). Thus available P as $P_2O_5$(AVP) and P sorption (PS) were most important factors in determining the concentration of 0.01M $CaC1_2-P$($CaC1_2-P$). In multinomial equation related $CaC1_2-P$ with AVP and PS, the determination coefficient was improved to 0.745. The logarithm of $CaC1_2-P$ was linearly related to AVP/PS. Consequently, the equation, $0.01M\;CaCl_2-P=0.1284e^{0.3288AVP/PS}$ could be suggested to estimate the concentration of P in 20mL of 0.01M $CaCl_2$ solution containing 2g of soil shaken for 17 hours.

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Characteristics of the RNase from the moderate halophile, Micrococcus sp. (호염성 세균 Micrococcus Sp.가 생산하는 RNase의 특성)

  • Jeon, Byoung One;Kim, Chan Jo;Oh, Man Jin;Choi, Seong Hyun
    • Korean Journal of Agricultural Science
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    • v.21 no.1
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    • pp.11-21
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    • 1994
  • The halophile, Micrococcus sp. which produces RNase was isolated from salted and fermented food. The optimum growth condition of the Micrococcus sp. in pH 7.0 of complex medium containing 2M NaCl, and at $35^{\circ}C$. Optimum condition for enzyme production by this strain was when it was grown in the CM medium, containing 2% yeast extract, 1.5% casamino acid and 2M NaCl in the initial pH 8.5 for 2 days. The maximal RNase activity was observed at pH 8.0 and $55^{\circ}C$. The Km value for RNA was determined to be 5mg/ml by Lineweaver-Burk plot. The RNase activity in the absence of NaCl was maximum, but it was completely lost by adding of 1.25M NaCl and it was increased above 1.25M to 2.5M NaCl. When 2.5M NaCl was added, the activity of RNase showed 45% of maximum value.

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Cutaneous Leishmaniasis Situation and Predicting the Distribution of Phlebotomus papatasi and P. sergenti as Vectors of Leishmaniasis in Ardabil Province, Iran

  • Khamesipour, Ali;Molaei, Soheila;Babaei-Pouya, Navid;Moradi-Asl, Eslam
    • Parasites, Hosts and Diseases
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    • v.58 no.3
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    • pp.229-236
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    • 2020
  • Cutaneous leishmaniosis (CL) is the most common form of leishmaniasis.CL caused by L. major and L. tropica is endemic in 17 provinces of Iran. This study was carried out to elucidate situation of CL in Ardabil province and to predict distribution of Phlebotomus papatasi and Phlebotomus sergenti (Diptera: Psychodidae) as vectors of CL in the region. In this cross-sectional study, data on CL patients were collected from local health centers of Ardabil province, Iran during 2006-2018 to establish a geodatabase using ArcGIS10.3. A total of 20 CL cases were selected randomly and skin samples were collected and analyzed by PCR method. MaxEnt 3.3.3 model was used to determine ecologically suitable niches for the main vectors. A total, 309 CL human cases were reported and the highest incidence rate of disease was occurred in Bilasavar (37/100,000) and Germi (35/100,000). A total of 2,794 sand flies were collected during May to October 2018. The environmentally suitable habitats for P. papatasi and P. sergenti were predicted to be present in northern and central areas of Ardabil province. The most variable that contributed ratio in the modeling were Isothermality and slope factors. Ardabil province is possibly an endemic are for CL. The presence of P. papatasi and P. sergenti justifies local transmission while the vectors of CL are existing in the northern and central areas of the province.

Stability of Pigment Produced by Monascus pilosus (Monascus pilosus가 생성하는 색소의 안정성)

  • Park, Mee-Ja;Yoon, Eun-Kyung;Kim, Soon-Dong
    • Korean Journal of Food Science and Technology
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    • v.34 no.4
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    • pp.541-545
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    • 2002
  • Stability of Monascus pilosus pigment was investigated under various conditions. The concentration of the pigments stored under air and sub-atmosphere (250 mmHg) for 30 days at room temperature decreased by 77.9% and 48.4%, respectively. The pigment solution was stable under temperature ranges of $20-80^{\circ}C$, pH 4-8, darkness and presence of KCl, NaCl, $CaCl_2$, $MgCl_2$, and $ZnCl_2$. In contrast, the pigment solution was relatively unstable, decreasing in concentration by 6.0 and 11.6% at 100 and $121^{\circ}C$, 15.5 and 13.7% at pH 3 and 9, 22.9 and 66.8% under fluorescence and sun light, respectively, and 20.2% in the presence of $AlCl_3$.

The Influence of H+ and Cl- Ions on the Corrosion Inhibitive Effect of Poly(para-aminophenol) for Iron in Hydrochloric acid

  • Manivel, P.
    • Corrosion Science and Technology
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    • v.16 no.4
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    • pp.187-193
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    • 2017
  • Polymer amines are found to show distinct corrosion inhibition effects in acidic media. The functional groups of organic compounds have a wide role in the physical and chemical properties, for the inhibition efficiency with respect to steric factors, aromaticity, and electron density. The influence of $H^+$ ions and $Cl^-$ ions on the corrosion inhibitive effect of poly(p-aminophenol) for iron in hydrochloric acid was studied using electrochemical methods such as impedance, linear polarization, and Tafel polarization techniques. The experiments were conducted with and without the inhibitor, poly(p-aminophenol). The concentration range of $H^+$ ions and $Cl^-$ ions are from 1 M to 0.05 M and 1 M to 0.1 M, respectively. With the inhibitor poly(p-aminophenol), this study shows that inhibition efficiency decreases with the reduction of $H^+$ ion and $Cl^-$ ion concentrations in aqueous solution. Further, it reveals that the adsorption of an inhibitor on the surface of iron is dependent on the concentrations of $H^+$ and $Cl^-$ ions in the solution and the adsorption of inhibitor on the iron surface through the cationic form of amine.