• 한국동물학회지
• /
• 제34권3호
• /
• pp.420-425
• /
• 1991

본 연구는 인삼 사포닌(Ponax ginseng C.A. Meyer)이 수영운동을 부하시킨 웅성 횐쥐(Sprague-Dawley 계, 360 $\pm$ 40 9)의 대퇴근 크레아틴 키나제(Creative Kinase, CK: E.C. 2.7.3.2) 동위효소 활성에 미치는 영향을 연구하였다. 운동군은 3시간 동안 수영운동시켰으며 ,인삼군은 인삼 사포닌을 체중 Kg 당 120 mg 복강투여하였다. 인삼 사포닌을 투여한 운동군은 수영운동 1시간 전에 인삼 사포닌을 투여한 후 3시간 동안 수영시켰다. 인삼군의 MB-CK의 활성은 대조군보다 유의하게 증가하였다(P<0.01). 또한 인삼 비투여 운동군과 인삼 투여 운동군에서의 MM-CK활성은 대조군보다 각각 현저하게 증가하였고(P < 0.01와 P < 0.05), 특히 인삼 투여 운동군에서 BB-CK활성이 대조군보다 유의하게 증가하였다(P < 0.01). 인삼 사포닌은 일반적으로 CK-동위효소의 활성을 증가시키고, 운동은 MM-CK의 활성을 현저하게 증가시켰으며, 인삼 투여 운동군에서 BB-CK의 활성이 인삼 비투여 운동군보다 현저하게 증가하였다. 따라서 인삼 사포닌과 운동이 CK동위효소 활성에 상승의 효과를 나타내는 것으로 생각된다.

• Journal of Chest Surgery
• /
• 제33권2호
• /
• pp.117-124
• /
• 2000

Background: Nucleoside transport inhibitor(NTI) Keeps AMP, ADP, ATP levels high in myocytes by inhibiting adenosine cataboilsm so that it may preserve the myocardial contractability during ischemia In this study we investigated the effects of cyclic AMP phosphodiesterase inhibor(C-AMP PDSI) and S-P-nitrobenzyl-6 -thioniosine(NBT; a sort of NIT) on myocadial preservation and changes of constituent enzyme. Material and method: Twenty-six isolated rabbit hearts were perfused with Krebs-Henseleit buffer solution for 20 minutes arrested for 20 minutes and ten reperfused for 30 minutes. The following four groups were prepared and hemodynamic changes coronary effluent lactate dehydrogenase (LDH) a-hydroxybutylic accid(a-HBD) levels and myocardial LDH creatine kinase-MB (CK-MB) adenosine deaminase(ADA) a-HBD levels and myocardial LDH creatine kinase-MB (CK-MB) adenosine deaminase(ADA) a-HBD levels were analysed before and after cardiac arest ; Group I(control) ; the heart was only perfused with K-H ; Group II ; the heart was perfused with K-H including C-AMP PDSI(Amrinone 25mg/L); Group III ; the heart was perfused with K-H including NBT(4.19mg/L) ; Group IV ; the heart was perfused with K-H including C-AMP PDSI + NBT. Result : Left venticular developed pressure(LVDP) at 10 minutes of the equilibrium was significantly higher in group III(72.1$\pm$5.3 mmHg p<0.01) and group III(72$\pm$5.6 mmHg P<0.025) as compared with group I (40.8$\pm$4.7mmHg) and LVDP at 20 minutes of the reperfusion was significantly higher in group II(74$\pm$5.3mmHg p<0.01) and group III(72$\pm$5.6mmHg p<0.025) as compared with group I (44.2$\pm$4.6mmHg). Percentage recovery of LVDP at the reperfusion was the highest in group II(123.3%) Percentage recovery of coronary flow at the equilibrium reperfusion were higher in group II(310%, 270%) group III(230%, 290%) group IV(310%, 280%) as compared with group I (100%) respectively. Myocadial LDH level was significant lower in group IV(33495$\pm$1802 IU/gm p<0.04) as compared with group I(48767$\pm$1421 IU/gm) Myocadial CK-MB level was significant higher in group II(74820$\pm$1421 IU/gm) compared with group I (45450$\pm$1737 IU/gm) Myocadial ADA level was significant higher group IV(1215$\pm$8 IU/gm p<0.05) compared with group I(125$\pm$15 IU/gm) but there was no significant difference between group I and group II ,III, IV in changes of coronary effluent LDH, a-HBD levels. Conclusion: C-AMP PDSI solely appears to have a better effect on myocardial preservation after ischemia than NBT but with no synergistic effect and it could keep CK-MB leve high in myocardial tissues.

• Journal of Chest Surgery
• /
• 제31권1호
• /
• pp.20-27
• /
• 1998

Troponin T(이하 TnT)는 주로 심근세포에 존재하며 심근손상 시에만 유리되는 심근특이 효소이다. 저자들은 개심술을 시행한 34명의 환자들을 대상으로 수술 전후 심근손상에 대한 지표로서 TnT의 유용성에 대해 조사하였다. 환자들은 허혈성 심질환에 의한 관동맥 우회술이 11례, 후천성 판막 질환이 13례, 그리고 선천성 심질환이 10례였다. 이들은 술후 흉통과 심전도의 ST분절과 T파의 변화 등에 의거하여 심근손상이 의심되는 환자들(A군, 11례)과 정상 소견을 가진 환자들(B군, 23례)로 구분하였다. TnT의 측정은 효소 면역법을 이용하여 술 전, 수술 직후와 술후 1, 2, 3, 7일에 각각 측정하여 수술 전후의 수치 변화를 각 군간에 서로 비교하였고, 같은 시기에 측정한 CK-MB의 변화와 비교 분석하였다. A군에서는 수술 직후와 술후 1, 2, 3, 7일에 각각 1.37$\pm$0.26 $\mu$g/L, 3.16$\pm$0.66 $\mu$g/L, 2.39$\pm$0.74 $\mu$g/L, 2.49$\pm$0.76 $\mu$g/L, 그리고 1.23$\pm$0.60 $\mu$g/L로 변화하였으며, B군에서는 0.38$\pm$0.04 $\mu$g/L, 0.34$\pm$0.05 $\mu$g/L, 0.25$\pm$0.03 $\mu$g/L, 0.24$\pm$0.04 $\mu$g/L, 그리고 0.11$\pm$0.03 $\mu$g/L로 두 군간에 전기간에 걸쳐서 유의한 차이를 가졌다(P<0.01). 이에 반하여 CK-MB는 술후 1일째만 A군과 B군간에 145.04$\pm$35.08 IU/L과 31.28$\pm$5.87 IU/L로 유의한 차이가 있었고(P<0.05), 2일째부터 급격히 감소하여 3일 이후에는 술전치로 환원하였다. 혈청 TnT치가 1.0 $\mu$g/L이상인 경우를 술후 심근손상의 기준으로 볼 때 TnT는 심근손상의 진단에 있어서 100%의 민감도와 87%의 특이도를 보였다(P<0.01). 따라서 TnT는 심근손상 환자에 있어서 CK-MB와 달리 수술 직후부터 증가하여 지속됨으로써 심근손상의 정도를 더욱 민감하게 반영하고, 장기간 유의한 차이를 보여줌으로써 술후의 심근손상을 진단하는데 있어서 기존의 방법들보다 더욱 유용한 것으로 사료된다.

• The Korean Journal of Physiology and Pharmacology
• /
• 제20권6호
• /
• pp.565-571
• /
• 2016

Paeoniflorin (PAE) is the most abundant compound in Xuebijing injection widely used to treat sepsis. We aimed to investigate effect of PAE on expression of soluble triggering receptor expressed on myeloid cells-1 (sTREM-1) in a rat model of sepsis. Wistar rats were divided into Normal, Model, and PAE groups (n=20 each). Endotoxin was administrated at 5 mg/ml/kg in Model and PAE rats to establish rat sepsis model. 1 h after endotoxin administration, PAE was administrated at 4 ml/kg in PAE group once per day for 3 days. Routine blood tests and biochemical indexes were assessed, including aspartate aminotransferase (AST) and creatine kinase-MB (CK-MB). The plasma sTREM-1 level was measured using quantitative ELISA. At the end of experiment, the small intestine, liver, kidney and lung were subjected to pathological examinations. A rat model of sepsis-induced multiple organ dysfunction syndrome (MODS) was established successfully with endotoxin administration (5 mg/ml/kg), evidenced by histo-pathological examinations, routine blood tests and biochemical indexes: platelet count decreased and white blood cell count increased (p<0.05), CK-MB and AST increased (p<0.05). PAE treatment significantly reduced the plasma levels of AST, CK-MB, and sTREM-1, compared to Model group (p<0.05). Meanwhile, sepsis-induced damages in the liver, lung, stomach and intestinal mucosa were also markedly ameliorated by PAE treatment. PAE demonstrated a significantly protective effect in a rat model of sepsis by decreasing plasma sTREM-1 level, reducing inflammation, preventing MODS and protecting organ functions.

• The Korean Journal of Physiology and Pharmacology
• /
• 제21권6호
• /
• pp.651-656
• /
• 2017

Ursolic acid (UA) supplementation was previously shown to improve skeletal muscle function in resistance-trained men. This study aimed to determine, using the same experimental paradigm, whether UA also has beneficial effects on exercise-induced skeletal muscle damage markers including the levels of cortisol, B-type natriuretic peptide (BNP), myoglobin, creatine kinase (CK), creatine kinase-myocardial band (CK-MB), and lactate dehydrogenase (LDH) in resistance-trained men. Sixteen healthy participants were randomly assigned to resistance training (RT) or RT+UA groups (n=8 per group). Participants were trained according to the RT program (60~80% of 1 repetition, 6 times/week), and the UA group was additionally given UA supplementation (450 mg/day) for 8 weeks. Blood samples were obtained before and after intervention, and cortisol, BNP, myoglobin, CK, CK-MB, and LDH levels were analyzed. Subjects who underwent RT alone showed no significant change in body composition and markers of skeletal muscle damage, whereas RT+UA group showed slightly decreased body weight and body fat percentage and slightly increased lean body mass, but without statistical significance. In addition, UA supplementation significantly decreased the BNP, CK, CK-MB, and LDH levels (p<0.05). In conclusion, UA supplementation alleviates increased skeletal muscle damage markers after RT. This finding provides evidence for a potential new therapy for resistance-trained men.

• JSTS:Journal of Semiconductor Technology and Science
• /
• 제7권3호
• /
• pp.151-160
• /
• 2007

We report the label-free biomolecules detection based on nanomechanical micro cantilevers operated in dynamic mode for detection of two marker proteins (myoglobin and creatin kinase-MB (CK-MB)) of acute myocardical infarctions. When the specific binding between the antigen and its antibody occurred on the fuctionalized microcantilever surface, mechanical response (i.e. resonant frequency) of microcantilevers was changed in lower frequency range. We performed the label-free biomolecules detection of myoglobin and CK-MB antigen in the low concentration (clinical threshold concentration range) as much as 1 ng/ml from measuring the dynamic response change of micro cantilevers caused by the intermolecular force. Moreover, we estimate the surface stress on the dynamic microcantilevers generated by specific antibody-antigen binding. It is suggested that our dynamic microcantilevers may enable one to use the sensitive label-free biomolecules detection for application to the disease diagnosis system based on mechanical immuno-sensor.

• The Korean Journal of Physiology and Pharmacology
• /
• 제26권6호
• /
• pp.511-518
• /
• 2022

Sepsis-associated myocardial injury, an invertible myocardial depression, is a common complication of sepsis. Neogambogic acid is an active compound in garcinia and exerts anthelmintic, anti-inflammatory, and detoxification properties. The role of neogambogic acid in sepsis-associated myocardial injury was assessed. Firstly, mice were pretreated with neogambogic acid and then subjected to lipopolysaccharide treatment to induce sepsis. Results showed that lipopolysaccharide treatment induced up-regulation of biomarkers involved in cardiac injury, including lactate dehydrogenase (LDH), creatine kinase-MB (CK-MB), and troponin I (cTnI). However, pretreatment with neogambogic acid reduced levels of LDH, CK-MB, and cTnI, and ameliorated histopathological changes in the heart tissues of septic mice. Secondly, neogambogic acid also improved cardiac function in septic mice through reduction in left ventricular end-diastolic pressure, and enhancement of ejection fraction, fractional shortening, and left ventricular systolic mean pressure. Moreover, neogambogic acid suppressed cardiac apoptosis and inflammation in septic mice and reduced cardiac fibrosis. Lastly, protein expression of p-p38, p-JNK, and p-NF-κB in septic mice was decreased by neogambogic acid. In conclusion, neogambogic acid exerted anti-apoptotic, anti-fibrotic, and anti-inflammatory effects in septic mice through the inactivation of MAPK/NF-κB pathway.

• Journal of Ginseng Research
• /
• 제43권4호
• /
• pp.692-698
• /
• 2019

Background: Breast cancer is a severe disease and the second leading cause of cancer death in women worldwide. To surmount this, various diagnosis and treatment options for breast cancer have been developed. One of the most effective strategies for cancer treatment is to induce apoptosis using naturally occurring compounds. Compound K (CK) is a ginseng saponin metabolite generated by human intestinal bacteria. CK has been studied for its cardioprotective, antiinflammatory, and liver-protective effects; however, the role of CK in breast cancer is not fully understood. Methods: To investigate the anticancer effects of CK in SKBR3 and MDA-MB-231 cells, cell viability assays and flow cytometry analysis were used. In addition, the direct targets of CK anticancer activity were identified using immunoblotting analysis and overexpression experiments. Invasion, migration, and clonogenic assays were carried out to determine the effects of CK on cancer metastasis. Results: CK-induced cell apoptosis in SKBR3 cells as determined through 3-(4-5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide assays, propidium iodide (PI) and annexin V staining, and morphological changes. CK increased the cleaved forms of caspase-7, caspase-8, and caspase-9, whereas the expression of Bcl-2 was reduced by CK. In assays probing the cell survival pathway, CK activated only AKT1 and not AKT2. Moreover, CK inhibited breast cancer cell invasion, migration, and colony formation. Through regulation of AKT1 activity, CK exerts anticancer effects by inducing apoptosis. Conclusion: Our results suggest that CK could be used as a therapeutic compound for breast cancer.

• Journal of Chest Surgery
• /
• 제29권10호
• /
• pp.1055-1065
• /
• 1996

혈관확장의 기전으로 혈류를 증가시킨다고 보고된 nitric oxide(이하 NO), Isosorbide dinitrate(이하 ISDW), 그리고 sodium nitroprusside(이하 SWP)에 대해 어떤 물질이 허혈성 심근에서 더 효과적인지를 알아보기 위해 이 실험을 시행하였다. 그리고 실험군은 정상대조군(1군), WO군(2군), ISDW군(3군), SWP군(4군)으로 나누었다. 실험 대상은 쥐의 심근을 채택하였으며, 실험 모델은 isolated constant pressure Langendorfr system을 채택하였다. 그리고 효과를 비교하기 위해서 냉동 심근과 관혈류내의 lactate와 CK-MB수치, 관혈류량, LVDP, dp/dt등을 측정하였다. 결과는 12군과 4군에서 허혈전 상태에서의 lactate치가 낮았다(p<0.0025). 2. 반면에 관혈류량은 2군과 4군에서 높았다. 3. 허혈전과 허 혈후에 생산된 lactate수치의 비는 2군과 4군에서 낮았다(p< 0.005). 4.관혈류내의 CK-MB치는 2군과 3군에서 낮았으며,냉동 심장내의 CK-MB치는 2군과4군에서 낮았 다. 5. 관혈류량은 전 실험 과정 에서 2군과 4군에서 높았다(p<0.005) 6. 허혈전 상태 에서의 LWP는 2군에서 가장 높았다. 7. +maximal dp/dt는 2군에서 가장 높았다. 8. 허혈전과 재\ulcorner류후의 회복률은 1군이 가장 높았다. 9. (-dp/dt)1(+dp/dt)의 비는 각군에서 각각 116%, 100%, 100%, 55%였다. 0. total dp/dt의 회복률은 각각 34%, 67%, 51%, 76%였다 결론적으로 nitric oxide는 관상혈관의 확장에 상당한 효과가 있는것으로 생각되며, lactate와 CK-MB 의 생산은 NO 군과 ISDW군에서 효과적으로 낮았다.

• 대한영양사협회학술지
• /
• 제7권1호
• /
• pp.1-8
• /
• 2001

The purpose of this study was to investigate the relationship between nutrient intakes and blood parameters of cardiovascular function in 40 female college students on self-selected diet in Chungnam. Anthropometric measurements, analysis of dietary intakes and blood composition were conducted. Serum levels of triglyceride, total cholesterol, HDL-cholesterol, LDL-cholesterol, CK-MB, and LDH were measured by biochemical analyzer. The results were summarized as follows. The mean age of the subjects was 22.5$\pm$1.2 years. The weight, height and BMI were 52.4$\pm$7.2kg, 161.3$\pm$5.7cm and 20.2$\pm$2.4, respectively. Daily energy and lipid intakes were 1634.2$\pm$ 437.7kcal and 46.8$\pm$17.0g. And the ratio of carbohydrate, protein, and lipid to energy intake was 59.2 : 14.5 : 26.3. Serum concentrations appeared to be 61.1$\pm$ 24.2mg/dl(triglyceride), 141.2$\pm$330.mg/dl(total cholesterol), 64.8$\pm$ 20.1mg/dl(HDL-cholesterol), 64.1$\pm$ 25.4mg/dl(LDL-cholesterol), 7.2$\pm$3.2IU/l (CK-MB), 107.9$\pm$23.4IU/l (LDH). In the relation between nutrient intakes and serum parameters, vitamin $B_1$ intake showed positive correlation with triglyceride, HDL-cholesterol, and CK-MB, respectively(p<0.001, p<0.05, p<0.05). Vitamin $B_2$intake had positive correlation with triglyceride(p<0.05). And vitamin C intake had negative correlation with HDL-cholesterol(p<0.05). From these results, it is suggested that the intakes of micro nutrients such as vitamin $B_1$, vitamin $B_2$, vitamin C have relation with cardiovascular function in female college students. However, further systematic research is needed to investigate the functions of micro nutrients in healthy persons for prevention of chronic diseases.