• 디지털융복합연구
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• 제15권4호
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• pp.1-11
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• 2017

본 연구의 목적은 청소년들의 자기주도학습 능력 향상을 위한 SMMIS 모형 기반의 온라인 종합진단검사 도구를 개발하는 것이다. 이를 위해 문헌 분석 및 전문가 검토를 거쳐 초 중학생용과 고등학생용 검사 문항을 개발하였다. 아울러 해석 규준 설정을 위해 총 1,626명의 초, 중, 고등학생을 대상으로 검사를 실시하였다. 그 결과를 토대로 블렌디드러닝 기반 온라인 자기주도학습 종합진단검사 시스템을 개발하였다. 아울러 청소년 대상 블렌디드러닝 기반 온라인 종합진단검사 결과의 구체적 활용 방안과 향후 보완 및 후속 연구 방향에 대해 논의하였다.

• Journal of Ginseng Research
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• 제46권3호
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• pp.496-504
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• 2022

Background: Cigarette smoke (CS) is considered a principal cause of chronic obstructive pulmonary disease (COPD) and is associated with mucus hypersecretion and airway inflammation. Ginsenoside compound K (CK), a product of ginsenoside metabolism, has various biological activities. Studies on the effects of CK for the treatment of COPD and mucus hypersecretion, including the underlying signaling mechanism, have not yet been conducted. Methods: To study the protective effects and molecular mechanism of CK, phorbol 12-myristate 13-acetate (PMA)-induced human airway epithelial (NCI-H292) cells were used as a cellular model of airway inflammation. An experimental mouse COPD model was also established via CS inhalation and intranasal administration of lipopolysaccharide. Mucin 5AC (MUC5AC), monocyte chemoattractant protein-1, tumor necrosis factor-α (TNF-α), and interleukin-6 secretion, as well as elastase activity and reactive oxygen species production, were determined through enzyme-linked immunosorbent assay. Inflammatory cell influx and mucus secretion in mouse lung tissues were estimated using hematoxylin and eosin and periodic acid-schiff staining, respectively. PKCδ and its downstream signaling molecules were analyzed via western blotting. Results: CK prevented the secretion of MUC5AC and TNF-α in PMA-stimulated NCI-H292 cells and exhibited a protective effect in COPD mice via the suppression of inflammatory mediators and mucus secretion. These effects were accompanied by an inactivation of PKCδ and related signaling in vitro and in vivo. Conclusion: CK suppressed pulmonary inflammation and mucus secretion in COPD mouse model through PKC regulation, highlighting the compound's potential as a useful adjuvant in the prevention and treatment of COPD.

• Journal of Ginseng Research
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• 제31권1호
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• pp.23-33
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• 2007

Ginsenosides are one of the most well-known traditional herbal medicines frequently used for the treatment of cardiovascular symptoms in korea. The anti-ischemic effects of the mixture of ginsenoside $Rg_3$, and CK on ischemia-induced isolated rat heart were investigated through analyses of changes in hemodynamics ; blood pressure, aortic flow, coronary flow, and cardiac output. The subjects in this study were divided into four groups: normal control, the mixture of ginsenoside $Rg_3$ and CK, an ischemia-induced group without any treatment, and an ischemia-induced group treated with the mixture of ginsenoside $Rg_3$ and CK. There were no significant differences in perfusion pressure, aortic flow, coronary flow and cardiac output between them before ischemia was induced. The supply of oxygen and buffer was stopped for five minutes to induce ischemia in isolated rat hearts, and the mixture of ginsenoside $Rg_3$ and CK was administered during ischemia induction. Treatments of the mixture of ginsenoside $Rg_3$ and CK significantly prevented decreases in perfusion pressure, aortic flow, coronary flow, and cardiac output under ischemic conditions. In addition, hemodynamics (except heart rate) of the group treated with the mixture of ginsenoside $Rg_3$ and CK significantly recovered 60 minutes after reperfusion compared to the control group (mixture+ischemia vs ischemia - average perfusion pressure: 74.4${\pm}$2.97% vs. 85.1${\pm}$3.01%, average aortic flow volume: 49.11${\pm}$2.72% vs. 59.97${\pm}$2.93%, average coronary flow volume: 58.50${\pm}$2.81% vs. 72.72${\pm}$2.99%, and average cardiac output: 52.47${\pm}$2.78% vs. 63.11${\pm}$2.76%, p<0.01, respectively). These results suggest that treatment of the mixture of ginsenoside $Rg_3$ and CK has distinct anti-ischemic effects in ex vivo model of ischemia-induced rat heart.

• Journal of Ginseng Research
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• 제47권2호
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• pp.274-282
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• 2023

Background: Ginsenoside compound K (CK) stimulated activation of the PI3K-Akt signaling is one of the major mechanisms in promoting cell survival after stroke. However, the underlying mediators remain poorly understood. This study aimed to explore the docking protein of ginsenoside CK mediating the neuroprotective effects. Materials and methods: Molecular docking, surface plasmon resonance, and cellular thermal shift assay were performed to explore ginsenoside CK interacting proteins. Neuroscreen-1 cells and middle cerebral artery occlusion (MCAO) model in rats were utilized as in-vitro and in-vivo models. Results: Ginsenoside CK interacted with recombinant human PTP1B protein and impaired its tyrosine phosphatase activity. Pathway and process enrichment analysis confirmed the involvement of PTP1B and its interacting proteins in PI3K-Akt signaling pathway. PTP1B overexpression reduced the tyrosine phosphorylation of insulin receptor substrate 1 (IRS1) after oxygen-glucose deprivation/reoxygenation (OGD/R) in neuroscreen-1 cells. These regulations were confirmed in the ipsilateral ischemic hemisphere of the rat brains after MCAO/R. Ginsenoside CK treatment reversed these alterations and attenuated neuronal apoptosis. Conclusion: Ginsenoside CK binds to PTP1B with a high affinity and inhibits PTP1B-mediated IRS1 tyrosine dephosphorylation. This novel mechanism helps explain the role of ginsenoside CK in activating the neuronal protective PI3K-Akt signaling pathway after ischemia-reperfusion injury.

• Carbon letters
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• 제14권4호
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• pp.234-242
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• 2013

Potassium hydroxide-activated carbons (CK21, CK11, and CK12) were prepared from pistachio nutshells. Physicochemical properties of activated carbons were characterized by TGA, $pH_{pzc}$, Fourier transform infrared spectroscopy, scanning electron microscopy, and $N_2$-adsorption at $-196^{\circ}C$. The examinations showed that activated carbons have high surface area ranging between 695-1218 $m^2/g$, total pore volume ranging between 0.527-0.772 mL/g, and a pore radius around 1.4 nm. The presence of acidic and basic surface C-O groups was confirmed. Batch adsorption experiments were carried out to study the effects of adsorbent dosage, temperature, initial concentration of adsorbate, and contact time on deltamethrin adsorption by activated carbons. The kinetic studies showed that the adsorption data followed a pseudo-second order kinetic model. The Langmuir model showed a maximum adsorption capacity of 162.6 mg/g at $35^{\circ}C$ on CK12. Thermodynamic studies indicated that adsorption was spontaneous and increased with temperature, suggesting an endothermic process.

• 정보처리학회논문지:소프트웨어 및 데이터공학
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• 제5권1호
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• pp.43-50
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• 2016

본 논문에서는 ASM(Active Shape Model) 특징점(Landmark)을 이용하여 정밀한 얼굴영역을 획득하고, 외형기반 접근법으로 표정을 인식하는 방법에 대하여 제안한다. 외형기반 표정인식은 EHMM(Embedded Hidden Markov Model) 및 이진패턴 히스토그램 특징과 SVM(Support Vector Machine)을 사용하는 알고리즘으로 구성되며, 제안 방법의 성능평가는 공인 CK 데이터베이스와 JAFFE 데이터베이스를 이용하여 수행되었다. 더불어, 성능비교는 기존의 눈 거리 기반의 얼굴 정규화 방법과 비교를 통하여 수행되었고, 또한 ASM 전체 특징점 및 변형된 특징을 SVM으로 인식하는 기하학적 표정인식 방법론과 성능비교를 수행하였다. 실험 결과, 제안 방법은 거리기반 얼굴정규화 영상을 사용한 방법보다 CK 데이터베이스 및 JAFFE 데이터베이스 경우, 최대 6.39%와 7.98%의 성능향상을 보였다. 또한, 제안 방법은 기하학적 특징점을 사용한 방법보다 높은 인식 성능을 보였으며, 이로부터 제안하는 표정인식 방법의 효용성을 확인하였다.

• 한국환경농학회지
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• 제32권3호
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• pp.231-239
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• 2013

본 연구는 병저항성 OsCK1 유전자와 제초제저항성 PAT 유전자가 도입된 병저항성 GM벼에서 도입 유전자의 발현 검증과 수서환경의 비표적 생물체인 잉어와 미꾸리에 미치는 영향을 확인하기 위해 수행되었다. 병저항성 GM벼에 도입된 OsCK1와 PAT 유전자의 PCR 분석 결과, 병저항성 GM벼에서만 특이적인 밴드가 검출되었으며, PAT 단백질 발현량을 ELISA 분석한 결과, 병저항성 GM벼에서만 $45.44{\pm}2.23{\mu}g/g$ 수준으로 검출되었고, 모품종인 낙동벼에서는 검출되지 않았다. 병저항성 GM벼와 낙동벼의 미꾸리(Misgurnus anguillicaudatus)와 잉어(Cyprinus carpio)에 대한 급성독성시험을 실시한 결과, 48시간 및 96시간-$LC_{50}$ 은 5,000 mg/L 이상으로 나타났다. 48시간 및 96시간 무영향농도 NOEC)는 5,000 mg/L이었다. 급성독성 시험기간 중 병저항성 GM벼와 낙동벼간의 pH, DO, 수온, 체중 및 전장에 대한 유의적인 결과는 나타나지 않았다.

• BMB Reports
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• 제39권4호
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• pp.426-431
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• 2006

Embryonic stem cells (ESCs), representing a population of undifferentiated pluripotent cells with both self-renewal and multilineage differentiation characteristics, are capable of spontaneous differentiation into cardiomyocytes. The present study sought to define the kinetic characterization of lactate dehydrogenase (LDH) and creatine kinase (CK) of ESC- and neonatal-derived cardiomyocytes. Spontaneously differentiated cardiomyocytes from embryoid bodies (EBs) derived from mouse ESC line (Royan B1) and neonatal cardiomyocytes were dispersed in a buffer solution. Enzymes were extracted by sonication and centrifugation for kinetic evaluation of LDH and CK with spectrophotometric methods. While a comparison between the kinetic properties of the LDH and CK of both groups revealed not only different Michaelis constants and optimum temperatures for LDH but also different Michaelis constants and optimum pH for CK, the pH profile of LDH and optimum temperature of CK were similar. In defining some kinetic properties of cardiac metabolic enzymes of ESC-derived cardiomyocytes, our results are expected to further facilitate the use of ESCs as an experimental model.

• Journal of Ginseng Research
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• 제43권3호
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• pp.460-474
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• 2019

Background: Ginsenoside compound K (CK) is a promising drug candidate for rheumatoid arthritis. This study examined the impact of polymorphisms in NR1I2, adenosine triphosphate-binding cassette (ABC) transporter genes on the pharmacokinetics of CK in healthy Chinese individuals. Methods: Forty-two targeted variants in seven genes were genotyped in 54 participants using Sequenom MassARRAY system to investigate their association with major pharmacokinetic parameters of CK and its metabolite 20(S)-protopanaxadiol (PPD). Subsequently, molecular docking was simulated using the AutoDock Vina program. Results: ABCC4 rs1751034 TT and rs1189437 TT were associated with increased exposure of CK and decreased exposure of 20(S)-PPD, whereas CFTR rs4148688 heterozygous carriers had the lowest maximum concentration ($C_{max}$) of CK. The area under the curve from zero to the time of the last quantifiable concentration ($AUC_{last}$) of CK was decreased in NR1I2 rs1464602 and rs2472682 homozygous carriers, while $C_{max}$ was significantly reduced only in rs2472682. ABCC4 rs1151471 and CFTR rs2283054 influenced the pharmacokinetics of 20(S)-PPD. In addition, several variations in ABCC2, ABCC4, CFTR, and NR1I2 had minor effects on the pharmacokinetics of CK. Quality of the best homology model of multidrug resistance protein 4 (MRP4) was assessed, and the ligand interaction plot showed the mode of interaction of CK with different MRP4 residues. Conlusion: ABCC4 rs1751034 and rs1189437 affected the pharmacokinetics of both CK and 20(S)-PPD. NR1I2 rs1464602 and rs2472682 were only associated with the pharmacokinetics of CK. Thus, these hereditary variances could partly explain the interindividual differences in the pharmacokinetics of CK.

• 한국구조물진단유지관리공학회 논문집
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• 제21권6호
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• pp.106-112
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• 2017

본 연구에서는 콘크리트 시공줄눈 면의 전단마찰 내력을 합리적으로 평가하기 위하여 콘크리트 소성론의 상계치 이론에 기반한 수학적 모델을 제시하였다. 전단면에서 횡보강근의 전단전달에 대한 과대평가를 피하기 위하여 시공줄눈 면에서의 하중전달에 대한 스트럿-타이 모델에서 콘크리트 할렬 및 압괴의 한계상태로부터 전단마찰 내력의 상한값을 유도하였다. 제시된 모델은 시공줄눈 면에서 콘크리트 점착력과 마찰계수를 거친 면의 경우 각각 $0.27(f_{ck})^{0.65}$와 0.95를, 부드러운 면의 경우 각각 $0.11(f_{ck})^{0.65}$와 0.64로 결정하였는데, 여기서 $f_{ck}$는 콘크리트 압축강도이다. 직접전단에 대한 기존 문헌으로부터 수집한 146 실험데이터와의 비교로부터, 제시된 모델은 AASHTO 및 fib 2010 식에 비해 예측 값과 실험 값들의 비의 표준편차 및 변동계수에 대해 더 낮은 값을 보였다. 특히 전단마찰 내력 평가에서 기준식들의 상당한 과소평가 경향과 달리 제시된 모델은 실험결과와 잘 예측하였다.