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http://dx.doi.org/10.5338/KJEA.2013.32.3.231

Responses of Misgurnus anguillicaudatus and Cyprinus carpio Fed on Disease Resistant(OsCK1) Rice Variety  

Oh, Sung-Dug (National Academy of Agricultural Science, Rural Development Administration)
Lee, Kijong (National Academy of Agricultural Science, Rural Development Administration)
Park, Soo-Yun (National Academy of Agricultural Science, Rural Development Administration)
Lee, Dae-Yong (Korea Testing & Research Institute)
Sohn, Soo-In (National Academy of Agricultural Science, Rural Development Administration)
Kim, Min-Young (National Academy of Agricultural Science, Rural Development Administration)
Ryu, Tae-Hun (National Academy of Agricultural Science, Rural Development Administration)
Publication Information
Korean Journal of Environmental Agriculture / v.32, no.3, 2013 , pp. 231-239 More about this Journal
Abstract
BACKGROUND: The disease resistant (OsCK1) rice was generated by inserting choline kinase (CK1) and phosphinothricin acetyltransferase (PAT) genes isolated from Oriza sativa and Streptomyces hygroscopicus into the genome of rice (Nakdongbyeo). With the potential problems of safety, the non-target organism evaluation is required as an essential element for the environmental risk assessment of genetically modified (GM) crops. In present study, we studied the effects on survival of Misgurnus anguillicaudatus and Cyprinus carpio, commonly used as a model organism in ecotoxicological studies. METHODS AND RESULTS: The M. anguillicaudatus and C. carpio were fed on disease resistant (OsCK1) rice and non-genetically modified (non-GM) rice (Nakdongbyeo) to 0, 10, 100, 1,000 and 5,000 mg/L, as treatment concentration respectively. The OsCK1 rice used for the test was confirmed to have the OsCK1/PAT gene expression by the PCR and ELISA analysis. Feeding test showed that no significant differences in cumulative immobility and abnormal response of M. anguillicaudatus and C. carpio fed on between OsCK1 rice and non-GM rice. The 96hr-$LC_{50}$ values showed no difference between OsCK1 rice (>5,000 mg/L) and non-GM rice (>5,000 mg/L). CONCLUSION(S): The results of this study suggested that there was no significant difference in toxicity for M. anguillicaudatus and C. carpio between OsCK1 rice and non-GM counterparts.
Keywords
Cyprinus carpio; Disease resistant transgenic rice; Misgurnus anguillicaudatus; Risk assessment;
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