• Food Science and Biotechnology
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• v.16 no.2
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• pp.205-211
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• 2007

This study was designed to investigate the suppressive effect of chlorophyll a on nitric oxide (NO) production and intracellular oxidative stress. In addition, chlorophyll a regulation of nuclear factor (NF) ${\kappa}B$ activation and inducible NO synthase (iNOS) expression were explored as potential mechanisms of NO suppression in a lipopolysaccharide (LPS)-stimulated macrophage cell line. RAW 264.7 murine macrophages were preincubated with various concentrations ($0-10\;{\mu}g/ mL$) of chlorophyll a and stimulated with LPS to induce oxidative stress and inflammatory response. Treatment with chlorophyll a reduced the accumulation of thiobarbituric acid-reactive substances (TBARS), enhancing glutathione level and the activities of antioxidative enzymes including superoxide dismutase, catalase, glutathione peroxidase (GSH-px), and glutathione reductase in LPS-stimulated macrophages compared to LPS-only treated cells. NO production was significantly suppressed in a dose-dependent manner (p<0.05) with an $IC_{50}$ of $12.8\;{\mu}g/mL$. Treatment with chlorophyll a suppressed the levels of iNOS protein and its mRNA expression. The specific DNA binding activities of NFkB on nuclear extracts from chlorophyll a treated cells were significantly suppressed in a dose-dependent manner with an $IC_{50}$ of $10.7\;{\mu}g/mL$. Chlorophyll a ameliorates NO production and iNOS expression through the down-regulation of NFkB activity, which may be mediated by attenuated oxidative stress in RAW 264.7 macrophages.

• Korean Journal of Food Science and Technology
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• v.19 no.6
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• pp.463-470
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• 1987

In order to develop a rapid predicting method of the shelf-life of moisture sensitive foods and establish their proper packaging methods, the qualify changes and shelf-life of dired laver as a model food were studied by the computer simulation. A mathematical model of the relationship between the rate constants of chlorophyll a and water activity was established at $10^{\circ}C,\;25^{\circ}C$ and $40^{\circ}C$. Computer simulation to predict water activity and chlorophyll a was developed by considering the simultaneous influence of storage conditions such as water content of products, storage temperature and relative humidity, packaging materials. Simulated quality changes of dried laver was in good agreement with the experiment data. Chlorophyll a and sensory score decreased as the water activity increased. And correlation coefficient between the sensory scores and the contents of chlorophyll a was very high as 0.991. The critical water activity by sensory evaluation was around 0.55. The shelf-life of dried laver packaged with plastic films could be predicted from the above results in various storage conditions.

• Environmental Engineering Research
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• v.25 no.4
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• pp.515-521
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• 2020

Nowadays, due to various pollution sources, it is essential for environmental scientists to monitor water quality. Phytoplanktons form the end of the food chain in water bodies and are one of the most important biological indicators in water pollution studies. Chlorophyll-A, a green pigment, is found in all phytoplankton. Chlorophyll-A concentration indicates phytoplankton biomass directly. Therefore, Chlorophyll-A is an indirect indicator of pollutants, including phosphorus and nitrogen, and their refinement and control are important. The present study, Moderate Resolution Imaging Spectroradiometer (MODIS) satellite images were used to estimate the chlorophyll-A concentration in southern coastal waters in the Caspian Sea. For this purpose, Multi-layer perceptron neural networks (NNs) were applied which contained three and four feed-forward layers. The best three-layer NN has 15 neurons in its hidden layer and the best four-layer one has 5 in each. The three- and four- layer networks both resulted in similar root mean square errors (RMSE), 0.1($\frac{{\mu}g}{l}$), however, the four-layer NNs proved superior in terms of R² and also required less training data. Accordingly, a four-layer feed-forward NN with 5 neurons in each hidden layer, is the best network structure for estimating Chlorophyll-A concentration in the southern coastal waters of the Caspian Sea.

• Proceedings of the KSRS Conference
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• v.2
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• pp.918-921
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• 2006

The short-term variation of sea surface temperature before and after typhoons and increase of chlorophyll a concentration that accompany with the typhoons during summer in the East/Japan Sea were explored by satellite. Four typhoons (NAMTHEUN, MEGI, CHABA and SONGDA) and a typhoon (NABI) passed over the East/Japan Sea in 2004 and 2005, respectively. Decreasing of SST was observed in the every five typhoons, however the magnitude of SST decreasing were various from 1 to $5^{\circ}C$. Chlorophyll a increases were found after the typhoons (0.1-3 ${\mu}g$ $l^{-1})$ except NAMTHEUN, and the area was approximately included in SST decreasing area by the typhoons. It suggests that chlorophyll a increase was caused by nutrient input from subsurface layer by strong mixing. On the other hand, rarely chlorophyll a increase was observed in northern area of polar frontal zone, which is located in $38-41^{\circ}N$, than northern area, and chlorophyll a increase in coastal area was higher (more than 3 times) than offshore area. It might suggest that chlorophyll a increase in the East/Japan Sea is also related with the depth or nitracline depth that affects the amount of nutrients supply to the upper layer by typhoon mixing.

• Korean Journal of Remote Sensing
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• v.18 no.3
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• pp.141-146
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• 2002

Chlorophyll-a concentration maps of Yongdam reservoir in September and October, 2001 were produced using Landsat ETM imagery and the in-situ water quality measurement data. In-situ water samples were collected on 16th September and 18th October during the satellite overpass. The correlations between the DN values of the imagery and the values of chlorophyll-a concentration were analyzed. The visible bands(band 1, 2, 3) and the near infrared band(band 4) data of September image showed the correlation coefficient values higher than 0.9. The October image showed correlation coefficient values of about 0.7 due to the low variations of chlorophyll-a concentration. Regression models between the DN values of the Landsat ETM image and the chlorophyll-a concentration have been developed for each image. The developed regression models were then applied to each image, and finally the chlorophyll-a distribution maps of Yongdam reservoir were produced. The produced maps showed the spatial distribution of the chlorophyll-a in Yongdam reservoir in a synoptic way so that the tropic state could be easily monitored and analysed in the spatial domain.

• Ocean and Polar Research
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• v.30 no.3
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• pp.289-301
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• 2008

Community structure of heterotrophic protists and their grazing impact on phytoplankton were studied in Northwest Pacific Ocean during October, 2007. The study area was divided into four regions based on physical properties (temperature and salinity) and chlorophyll-a distribution. They were Region I of North Equatorial Currents, Region II of Kuroshio waters, Region III of shelf mixed water, and Region IV of Tsushima warm current from East China Sea. The distribution of chlorophyll-a concentrations and community structure of heterotrophic protists were significantly affected by physical properties of the water column. The lowest concentration of chlorophyll-a was identified in Region I and II, where pico-sized chlorophyll-a was most dominant (>80% of total chlorophyll-a). Biomass of heterotrophic protists was also low in Region I and II. However, Region III was characterized by low salinity and temperature and high chlorophyll-a concentration, with relatively lower pico-sized chlorophyll-a dominance. The Highest biomass of heterotrophic protists appeared in Region III, along with the relatively less important nanoprotists. In Region I, II and IV, heterotrophic dinoflagellates were dominant among the protists, while ciliates were dominant in Region III. Community structure varied with physical(salinity and temperature) and biological (chlorophyll-a) properties. Biomass of heterotrophic protists correlated well with chlorophyll-a concentration in the study area ($r^2=0.66$, p<0.0001). The potential effect of grazing activity on phytoplankton is relatively high in Region I and II. Our result suggest that biomass and size structure of heterotrophic protists might be significantly influenced by phytoplankton size and concentration.

• ALGAE
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• v.24 no.3
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• pp.149-161
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• 2009

Characteristics of benthic microalgae and sediment properties were investigated for the intertidal flats of Kwangyang Bay, Korea. Sampling stations were selected every 100 m in the intertidal flats from land-side to open ocean at two different sampling sites. Samples were collected in June 2004, July, September, November, February and May 2005. Sediments properties were measured including temperature, water contents, sediment bulk density, nutrient concentrations in porewater. Chlorophyll a concentrations in surface sediment (0.5 cm) were measured and relationships between the chlorophyll a and various sediment properties were analyzed to identify major mechanisms regulating biomass of benthic microalgae in the intertidal flats using simple linear regression analysis. Sediment chlorophyll a concentrations were maximum during winter and minimum during warm seasons ranging from 4.4 mg $m^{-2}\;to\;81.2\;mg\;m^{-2}$. No clear spatial variations were observed for the sediment chlorophyll a in the study sites. Results from regression analysis suggested that benthic microalgae biomass was affected by sediment temperature and nutrients especially ammonium and silicate. Grazing effect was estimated using chlorophyll: pheopigments ratio, indirect indicator of grazing activity, and the positive correlation of the ratio and chlorophyll a implied that microalgae biomass is affected by grazing of zoobenthos although direct measurement of grazing activity is required to determine the importance of top-down controls in the benthic microalgae dynamics.

• Journal of Life Science
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• v.32 no.10
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• pp.743-748
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• 2022

The garlic chive (Allium tuberosum Rotter) is a prominent herb species in Asia and other nations of the world. Garlic chives is a favorite vegetable and used to garnish noodles in Korea. The effects of various doses of N, P, and K fertilizers and drought stress on the chlorophyll content in the leaves of garlic chives were investigated. The evaluations showed that chlorophyll a content was 0.386 at 10 mg/l N fertilizer and 0.584 at 50 mg/l N fertilizer. The treatment group showed a significant difference with regard to the contents of chlorophylls a and b and total chlorophyll at the 5% level (p<0.05). Pearson's correlation coefficient (Pearson's r) for chlorophylls a and b and total chlorophyll were 0.940, 0.966, and 0.971, respectively. The highest content of chlorophylls a and b and total chlorophyll in the leaves was recorded at 40 mg/l P fertilizer, while the values corresponding to 50 mg/l P fertilizer were lower than those for 40 mg/l P fertilizer. The content of total chlorophyll evaluated at 10 mg/l K fertilizer was 0.312 and that at 50 mg/l was 0.589. The simple linear regression showed the relationship between chlorophyll efficiency aand moisture. The slope factors of the dark-level fluorescence yield (Fo), the maximum fluorescence yield (Fm), the quenched state (Fv), and the maximal PSII quantum yield (Fv/Fm) for chlorophyll-efficient indicators were -0.931, 0.972, 972, and 0.950, respectively. NPK fertilizers and drought stress affected the chlorophyll content and efficiency of A. tuberosum.

• Journal of Plant Biology
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• v.14 no.1
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• pp.15-23
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• 1971

The productivity of summer phytoplankton communities in Lake Hwajinpo, Lake Yongrang and Lake Changja were studied by measuring vertical variation of chlorophyll a amounts. The author also classified the lake types on the basis of the amount of chlorophyll in the lake water. And in Lake Changja, the seasonal changes of stratification of chlorophyll were studies. In Lake Hwajinpo, the productive structure of the phytoplankton community in summer was found to be L-shaped and of the mesotrophic type. In Lake Yongrang, the productive structure of the phytoplankton community in summer was alo L-shaped and of the mesotrophic type. And maximum chlorophyll layer was near the lake bottom below the compensation depth. In Lake Changja, the structure of phytoplankton community in summer was reversed L-shaped and of the eutrophic type, with the maximum chlorophyll layer just below the surface. The vertical distribution of chlorophyll amounts as a measure of the productive structure almost always formed a stratum distribution except in September and sometimes in May, in Lake Changja. In September homogeneous distribution was observed.

• Journal of Korean Society for Geospatial Information Science
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• v.4 no.2 s.8
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• pp.35-45
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• 1996

The reflectance of chlorophyll was measured using UV-VIS spectrophotometer with the reflectance integrator in the laboratory in order to define its spectral characteristics. Sharp peaks appear at around 485nm and 655nm due to fluorescence and scattering, and the reflectance of chlorophyll increases at 580nm. With the increase in the chlorophyll concentration, the reflectance also increases. We have applied TM data to the reflectance spectrum of chlorophyll and have developed two formula with which one can estimate the chlorophyll concetration. Satellite re sensing, with its synoptic overage, is used to obtain the chlorophyll concentration in Daecheong reservoir. The approach involved acquisition of water quality samples front boat simultaneous with Landsat 5 satellite overpass. The remotely-sensed data and the ground truth data were obtained oil 20 June 1995 and on 18 March 1996. Regression models have been developed between the chlorophyll concentration and Landsat Thematic Mapper digital data. As the regression model was determined based on the correlation coefficient which was higher than 0.7 and the spectral characteristics of chlorophyll, and we have applied it to the entire study area to genelate a distribution map of trophic state. According to the trophic state map made based upon Aizaki's TSI and chlorophyll a concentration, the area where Okchun stream was flowing into was shown to be polluted the most all over the Daechung reservoir by showing an eutrophic state in June 1995 and a mesotrophic state in March 1996.