• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.26 no.2
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• pp.137-145
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• 2000

Carcinogenesis is a multi-stage process that generally consists of at least three steps; initiation, promotion, and progression. If one of these carcinogenic steps were suppressed or delayed, the cancer could be prevented. Cancer chemoprevention is defined to be inhibition or reversal of the carcinogenic process by the specific chemical agents and is a novel approach to cancer management alternative to conventional chemotherapy. Chlorophylln(CHL), a water-soluble derivative of chlorophyll, containing sodium and copper, has been known to be strong antimutagen in several test systems, but its mechanism of antimutagenic action is unknown. In the present experiment, the possibility of CHL as chemopreventive drugs on 7,12-dimethylbenz[a]anthracene(DMBA)-induced hamster buccal pouch carcinogenesis was investigated by mutagenicity test, carcinogenicity test, and frequency or spectrum of H-ras mutations in the both of DMBA-induced and chlorophylln-pretreated-DMBA induced tumor by polymerase chain reaction and non-isotopic restriction fragment length polymorphism. The treatment of CHL reduced the yields and multiplicity of the 0.5% DMBA-induced tumor, 86% to 62.5% and $3.7{\pm}0.6$ to $1.4{\pm}0.3$, respectively. The occurrence of histidine revertant by $20{\mu}mole$ DMBA was inhibited 25.6 to 81.7% by 1 to $5{\mu}M$ CHL in a dose-dependent manner. The mutation rates of H-ras gene in DMBA-induced and CHL-pretreated-DMBA induced tumor were 96%, 94% of which the most mutations were in codon 12/13. These results suggest that CHL inhibits the carcinogenic action of DMBA by the formation of complex between CHL and DMBA or the inhibition of the activation of DMBA in vivo. But CHL did not affect the mutation rates or its spectrum in already formed tumor.

• Korean Journal of Ecology and Environment
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• v.40 no.1
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• pp.14-30
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• 2007

The purpose of this study was to evaluate trophic conditions of various Korean reservoirs using Trophic State Index (TSI) and predict the reservoir conditions by empirical models. The water quality dataset (2000, 2001) used here were obtained from the Ministry of Environment, Korea. The water quality, based on multi-parameters of dissolved oxygen (DO), biological oxygen demand (BOD), chemical oxygen demand (COD), total phosphorus (TP), total nitrogen (TN), suspended solid (SS), Secchi depth (SD), chlorophyll-${\alpha}$ (CHL), and conductivity largely varied depending on the sampling watersheds and seasons. In general, trophic conditions declined along the longitudinal axis of headwater-to-the dam and the largest seasonal variations occurred during the summer monsoon of July-August. Major inputs of TP occurred during the monsoon (r=0.656, p=0.002) and this pattern was similar to solid dynamics of SS (r=0.678, p<0.001). Trophic parameters including CHL, TP, SD, and TN were employed to evaluate how the water systems varies with season. Trophic State Index (TSI, Carlson, 1977), based on TSI (CHL), TSI (TP), and TSI (SD), ranged from mesotrophic to eutrophic. However, the trophic state, based on TSI (TN), indicated eutrophic-hypereutrophic conditions in the entire reservoirs, regardless of the seasons, indicating a N-rich system. Overall, nutrient data showed that phosphorus was a primary factor regulating the trophic state. The relationships between CHL (eutrophication index) vs. trophic parameters (TN, TP, and SD) were analysed to develop empirical models which can predict the trophic status. Regression analyses of log-transformed seasonal CHL against TP showed that the value of $R^2$ was 0.31 (p=0.017) in the premonsoon but was 0.69 (p<0.001) during the postmonsoon, indicating a greater algal response to the phosphorus during the postmonsoon. In contrast, SD had reverse relation with TP, CHL during all season. TN had weak relations with CHL during all seasons. Overall, data suggest that TP seems to be a good predictor for algal biomass, estimated by CHL, as shown in the empirical models.

• Journal of the Korean Society of Food Science and Nutrition
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• v.3 no.1
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• pp.43-46
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• 1974

It was reported that the digestion ratio of chlorella was low because it had a low metabolic rate in body. Generally, the thickness of a cell membrane of it is $200-250\;{\AA}$, the weight of it is approximatly 13% of the total weight of a dry cell. And it is composed of protein, lipid, hemicellulose and ash etc. So, in order to elevate the digestion ratio of chlorella in body, we experimented the crude treatment methods of chlorella. The results obtained in this experiment are summarized as follows : 1. The digestion ratios calculated from ordinary N- balance method were 83.05% for 10% chl. (b) plus diastase group ; 81.25% for 10% chl. (b) plus amylase group, and 79.23% for 10% chl. (b), 58.55% for 10% chl. (a). 2. Biological values from this method were 80.25% for 10% chl. (b) plus diastase group, and 60% for 10% for chlorella(a).

• Journal of Korean Society on Water Environment
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• v.29 no.3
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• pp.377-382
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• 2013

Enriching anammox bacteria (AMX) in a lab-scale granular sequencing batch reactor using local digester centrate, we observed the significant enrichment of the filamentous-like bacterial population. These bacteria were revealed as novel bacterial species (termed CHL) belonging to Chlorobi/Bacteroidetes phyla via Denaturing Gradient Gel Electrophoresis (DGGE). Further, niche differentiation of AMX and CHL quantification was observed in granule and filament biomass, suggesting AMX was dominant in the granule and CHL was dominant in the filament. Therefore, it was confirmed the structural role of CHL was indeed to aid the granule formation of the AMX. In parallel, the physiological role of CHL was suspected to degrade biopolymers in the digester centrate using nitrate as an electron acceptor.

• Journal of the Korean Chemical Society
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• v.26 no.4
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• pp.224-228
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• 1982

The wavelength of chlorophyll-b on the absorbance and fluorescence emission were shifted to the longer depending on the increasing of solvent polarities but fluorescence excitation spectra were not. The presence of chl-b oligomers and monomers were identified by the specra of fluorescence emission. Fluorescence excitation, absorbance and the measurement of its intensities vs. the concentration of n-prOH added to chl-b solution. The calibration curve of chl-b solution were not obeyed to Beer's law in the range of concentrated soln. because of the presence as the oligomers.

• Korean Journal of Environmental Biology
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• v.22 no.3
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• pp.426-437
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• 2004

To understand the phytoplankton community in adjacent waters of Ulchin nuclear power plant (UNPP), abundance and the size fractionated $chl-\alpha$ concentrations were evaluated through seasonal interval sampling from April 2003 to February 2004. A total of 211 different phytoplankton species was observed and mean abundance of phytoplankton in each study period ranged from 244,286 to 1,221,779 cells $L^{-1}$. The contributions of microplankton $(>20\mu{m})$ to total phytoplankton abundance ranged from 42.5 to 83.6% (average 66.1%) and those of nanoplankton $(>20\mu{m})$ ranged from 16.4 to 57.5% (average 33.9%). Total chl-$\alpha$ concentrations of phytoplankton ranged from 0.52 to $2.26\mu{g}\;L^{-1}$. The contribution of chl-$\alpha$ concentrations of microplankton was higher than that of nano- and picoplankton through the study period with exception of July 2008. The results of abundances and $chl-\alpha$ concentrations suggest that microplankton has an important role in adjacent waters of UNPP. The diminution of abundances and $chl-\alpha$ concentrations of phytoplankton was observed after passage through the cooling water system, but it was gradually recovered by mixing with the ambient waters. Our results suggested that the influence of thermal discharges on phytoplankton should be restricted within narrow limits around outlet area of thermal effluents.

• Journal of The Korean Society of Agricultural Engineers
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• v.53 no.1
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• pp.63-69
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• 2011

The green algae Scenedesmus acuminatus was cultured in different media: animal wastewater and an artificial culture medium in order to evaluate potential use for tertiary treatment. The experiments were conducted with air flowrate 1~2 L/min at $28{\sim}30^{\circ}C$. The nitrogen and phosphorus showed very similar removal efficiencies (68~77 % and 69~80 % for nitrogen and phosphorus respectively). The optimal fed period was estimated as three days in the semi-continuous experiment. The effects of $CO_2$ (4.5 %) injection on nutrient uptake from animal wastewater (biological treatment effluent) were compared to an air injection under the same conditions of light and photoperiod. The uptake rates of nutrient with air injection were observed 0.009 gN/gChl-a/day, 0.028 gN/gChl-a/day and T-P 0.003 gP/gChl-a/day for nitrate, total nitrogen and phosphorus respectively. The rates were enhanced by addition of $CO_2$ to 0.026 gN/gChl-a/day, 0.076 gN/gChl-a/day and T-P 0.018 gP/gChl-a/day. This study establishes that $CO_2$ addition during nutrient deprivation of microalgal cells may accelerate tertiary wastewater treatment.

• Journal of Korean Society of Water and Wastewater
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• v.35 no.6
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• pp.507-516
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• 2021

In this study, the machine learning which has been widely used in prediction algorithms recently was used. the research point was the CD(chudong) point which was a representative point of Daecheong Lake. Chlorophyll-a(Chl-a) concentration was used as a target variable for algae prediction. to predict the Chl-a concentration, a data set of water quality and quantity factors was consisted. we performed algorithms about random forest and gradient boosting with Python. to perform the algorithms, at first the correlation analysis between Chl-a and water quality and quantity data was studied. we extracted ten factors of high importance for water quality and quantity data. as a result of the algorithm performance index, the gradient boosting showed that RMSE was 2.72 mg/m³ and MSE was 7.40 mg/m³ and R² was 0.66. as a result of the residual analysis, the analysis result of gradient boosting was excellent. as a result of the algorithm execution, the gradient boosting algorithm was excellent. the gradient boosting algorithm was also excellent with 2.44 mg/m³ of RMSE in the machine learning hyperparameter adjustment result.

• Journal of the Korean Society for Marine Environment & Energy
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• v.13 no.4
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• pp.223-233
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• 2010

Spatio-temporal variations of long-term water qualities (COD, SS, $Chl-{\alpha}$, N-related nutrients (TN, TDN, $NO_3^-$, $NH_4^+$), P-related nutrients (TP, TDP, $PO_4^{3-}$)) at two stations (St. SD, St. GG) in the Geumgang Reservoir were investigated from August 2001 to July 2008. Statistical methods such as t-test, factor analysis, and multi-regression analysis were applied to the water quality data in the reservoir as well as mass balances on TP. From the temporal comparisons of the water qualities between 2002 and 2007, average concentrations of $NH_4^+$, $PO_4^{3-}$, and TDP gradually decreased down by 60%, 24%, 52% in 2007. However, those of TP and $Chl-{\alpha}$ increased to 99% and 423% during the period. From the spatial comparisons between the two stations, St. GG showed higher concentrations for all of the N- and P-related nutrients than in St. SD, while opposite result for the $Chl-{\alpha}$. The factor analysis showed that "the seasonal variations of N- and P-related nutrients" were the two dominant factors occupying 49% of total variances of water qualities. Based on this result, multi-regression analysis executed for the two most influential parameters (TP and $Chl-{\alpha}$) focusing on the seasonal variations of these parameters: SS and $Chl-{\alpha}$ has contributed decisively to the concentrations of TP during the wet and dry season, respectively. On the other hand, COD and TP has been important for the $Chl-{\alpha}$ during the wet and dry season, respectively. From the established mass balances of TP loadings in the Geumgang Reservoir, Other Sources (60%) occupied the greatest contribution and Fluvial Input (38%) and Sediment (1%) during the wet season. However, both Fluvial Water (48%) and Other Sources (47%) supplied comparable amount of inputs and Sediment (5%) showed significantly increased input during the dry seasons. Recently especially during the dry winter seasons, migrating bird's excretion was estimated to contribute up to 8% of total TP input and 21% of Other Sources.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.7
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• pp.299-309
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• 2019

This study was designed to assess the quality and quantity of food resources such as the chlorophyll a concentration (Chl-a), the particulate organic carbon (POC) and the particulate organic nitrogen (PON), on blood cocke (Tegillarca granosa Linnaeus) farms from May to November 2017 in the northwestern area of Yeoja Bay, Korea. The values of Chl-a, POC and PON were $1.69-7.68{\mu}g{\cdot}L^{-1}$ (average: $3.48{\mu}g{\cdot}L^{-1}$ in the surface), 0.88-2.58 mM (average: 1.97 mM) and 0.17-0.90 mM (average: 0.54 mM), respectively, and these values were higher in the spring and summer and lower in the autumn. Furthermore, Chl-a and POC had higher values on the bottom layer than those vales on the surface, while PON had higher values on the surface than those values on the bottom. The POC/PON ratio and POC/Chl-a ratio were 1.56-7.88 (average: 3.71 on the surface of the water) and 216-967 (average: 700 on the bottom of the water), respectively, with most of the carbon sources being sediment-accumulated particle organic matter, and the contribution by phytoplankton was assessed as being low. These results show that the food source of the blood cockle farms in the northwest area of Yeoja Bay seems to be abundant in quantity, but this is considered to be very poor in quality.