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Simultaneous Enrichment of Novel Filamentous-Like Bacterial Population in Lab-Scale Granular Anaerobic Ammonia Oxidation (Anammox) Sequencing Batch Reactor  

Park, Hongkeun (Department of Earth and Environmental Engineering, Columbia University)
Publication Information
Journal of Korean Society on Water Environment / v.29, no.3, 2013 , pp. 377-382 More about this Journal
Abstract
Enriching anammox bacteria (AMX) in a lab-scale granular sequencing batch reactor using local digester centrate, we observed the significant enrichment of the filamentous-like bacterial population. These bacteria were revealed as novel bacterial species (termed CHL) belonging to Chlorobi/Bacteroidetes phyla via Denaturing Gradient Gel Electrophoresis (DGGE). Further, niche differentiation of AMX and CHL quantification was observed in granule and filament biomass, suggesting AMX was dominant in the granule and CHL was dominant in the filament. Therefore, it was confirmed the structural role of CHL was indeed to aid the granule formation of the AMX. In parallel, the physiological role of CHL was suspected to degrade biopolymers in the digester centrate using nitrate as an electron acceptor.
Keywords
Anaerobic ammonium-oxidizing bacteria; Bacterial population dynamics; Chlorobi/Bacteroidetes-like bacteria; Filamentous-like bacteria;
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