• KOREAN JOURNAL OF CROP SCIENCE
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• v.17
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• pp.45-78
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• 1974

To provide useful information for developing new high yielding soybean varieties and for improving cultural practices, an investigation was made on variation of dry matter production and on relationship among several agronomic characters of soybean plants grown under different planting times and densities as well as under different fertilizer levels, using Kwang-kyo, Dong puk-tae, and Suke ＃ 51 as determinate types and Shelby, SRF-300 and Harosoy as indeterminate types at the Crop Experiment Station during the period of 1972 and 1973. The results obtained were summarized as follow: 1. The dry weight, CGR and LAI at the initial flowering stage were high in the high plant population irrespective of varieties, planting times, and fertilizer levels. However, those characters of the indeterminate type were lower than those of the determinate types. The same characters of the indererminate type at the terminal leaf stage were either same or higher than those of the determinate types. 2. The dry weight of the determinate type at the initial flowering stage was similar to the indeterminate, type, when planting times were May 21 or June 15. The dry weights of both types of varieties were low when planted on July 10. When fertilizer levels were increased, the CGR, dry weight and LAI at the initial flowering stages were also increased. 3. Even though significant differences of LAI were obtained among the varieties within the same plant type, the indeterminate type was in general lower than that of the determinate type regardless of planting time and densities, or fertilizer levels, while the yield of the indeterminate type was comparable to the yield of the determinate type. 4. The high degree of leaf- and petiole-fall at the greenbean stage was highly associated with early planting and high levels of fertilizers. However, less amount of leaf- or petiole-fall was found when planted on July 10 or under low plant population. 5. The percent of stem weight was high under higher plant population, while the percent of leaf weight was high under lower plant population. When planting time was late, the percent of stem and petiole weight were reduced, while the leaf weight was increased. 6. The percent of pod weight of the determinate type at the terminal leaf stage was about 2% when planted on May 21, about 8% when planted on June 15, and about 9% when planted on July 10. The percent of pod weight of the indeterminate type at the terminal leaf stage were about 6 % when planted on May 21, 14% when planted on June 15 and 21% when planted on July 10. 7. Kwang kyo showed less degree of leaf-fall even when lodged due to high levels of fertilizer applied, while SRF-300 showed great damage due to lodging. 8. High yields were obtained when planted on May 21, but there were little yield differences between yields from May 21 and June 15 plantings. The reduction of yield due to late planting of July 10 was less apparent in the determinate type of varieties, while it was high in the indeterminate type. 9. The optimum plant population per are for high yield was 1, 250 to 2, 500 plants when planted on May 21, 2, 500 plants when planted on June 15, and 3, 333 plants when planted on July 10. 10. High correlation coefficients were obtained between dry matter weight and LAI at the terminal leaf stages, and between the dry matter weight and yield at the greenbean stages. The optimum dry weight for high yield in the determinate type was expected to be 25 kg. per are at the initial flowering stage and 50 kg. per are at the terminal leaf stage. In the indeterminate type the LAI and dry weight at the greenbean stage were 4 to 5 and 80 kg. per are, respectively. 11. Under the high plant population plant height was increased, while the stem diameter and the number of nodes and branches were reduced. Consequently, the percent of mainstem to main stem plus branches were increased, and the length of internode was also elongated. The ratios of stem weight, number of nodes and pods, and yield of main stem were increased when high plant population was associated with the early planting. The percent of main stem to branches for the indeterminate type was higher than that of the determinate type. 12. Under the high plant densities and late planting, the percent of the pod number and yields of main stem were increased, indicating that varieties with no or less branches were better adaptable under such conditions. 13. High degree of simple correlation coefficients was obtained between the LAI at the initial flowering stage and terminal leaf stage, and the total node number, dry matter and dry stem weight of both determinate and indeterminate types. Even though no significant correlation was found between the LAI at the initial flowering stage of the determinate type and the stem length and pod number per are, highly significant correlation coefficients were obtained between such characters in the indeterminate type of varieties. 14. The dry matter was positively correlated with the LAI, CGR, stem length, and pod number, node number and dry stem weight per are, while no significant correlation was found between the dry matter and stem diameter. 15. The correlation coefficients between lodging index and the LAI, dry weight, stem length and dry stem weight were highly significant. Negative correlation was obtained for the indeterminate type between the stem diameter and lodging index. The correlation coefficient between the stem diameter and lodging index was non-significant for the determinate type, while positive correlation was obtained between the yield and lodging index in the determinate type. The lodging index was also positively correlated with average length of internode of main stem. 16. The 100 seed weight appeared to be lowered under the high plant population and no fertilizer condition, and when planted late. Apparent differences of 100 seed weight were found between main stem and branches, being higher for the main stem than for the branches. 17. No variation of protein content was found due to different cultural practices. However, the oil content was apparently reduced when planted late.

• The Journal of Natural Sciences
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• v.5 no.1
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• pp.15-28
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• 1992

토양에 20년간 3요소+생고를 처리하였을때 토양의 화학적 성분, 공극율, 입단구조가 잘 발달되었다. 또한 이 처리에 의하여 CGR은 영화분화기때 가장 높았으나 무처리구나 3요소단용구에서는 전생육기간을 통하여 계속 증가하는 경향을 띈 반면 3요소+생고처리구에서는 영화분화기이후 계속 감소 하는 추세을 나타내고 있다. 현미수량에 있어서는 3요소+생고처리에 의하여 11%의 증가효과가 있었으며 이것은 주로 이삭의 일.이차기경의 분화수가 증가하였기 때문이었다. 또한 미립형태에 있어서도 완전미의 비율이 높았는데 이것은 등숙속도와 매우 밀접한 관계가 있어서 등숙속도가 빠를수록 심백미, 복백미 및 동할미가 많았다. 3요소 + 생고속도는 40DAH이었다.

• Proceedings of the KSAR Conference
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• 2000.10a
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• pp.10-12
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• 2000

Members of the glycoprotein family, which includes CG, LH, FSH and TSH, comprise two noncovalently linked $\alpha$- and $\beta$-subunits. Equine chorionic gonadotropin (eCG), known as PMSG, has a number of interesting and unique characteristics since it appears to be a single molecule that possesses both LH- and FSH-like activities in other species than the horse. This dual activity of eCG in heterologous species is of fundamental interest to the study of the structure-function relationships of gonadotropins and their receptors. CG and LH $\beta$ genes are different in primates. In horse, however, a single gene encodes both eCG and eLH $\beta$-subunits. The subunit mRNA levels seem to be independently regulated and their imbalance may account for differences in the quantities of $\alpha$ - and $\beta$ -subunits in the placenta and pituitary. The dual activities of eCG could be separated by removal of the N-linked oligosaccharide on the $\alpha$-subunit Asn 56 or CTP-associated O-linked oligosaccharides. The tethered-eCG was. efficiently secreted and showed similar LH-like activity to the dimeric eCG. Interestingly, the FSH-like activity of the tethered-eCG was increased markedly in comparison with the native and wild type eCG. These results also suggest that this molecular can implay particular models of FSH-like activity not LH-like activity in the eCG/indicate that the constructs of tethered molecule will be useful in the study of mutants that affect subunit association and/or secretion. A single-chain analog can also be constructed to include additional hormone-specific bioactive generating potentially efficacious compounds that have only FSH-like activity. The LH/CG receptor (LH/CGR), a membrane glycoprotein that is present on testicular Leydig cells and ovarian theca, granulosa, luteal, and interstitial cells, plays a pivotal role in the regulation of gonadal development and function in males as well as in nonpregnant and pregnant females. The LH/CGR is a member of the family of G protein-coupled receptors and its structure is predicted to consist of a large extracellular domain connected to a bundle of seven membrane-spanning a-helices. The LH/CGR phosphorylation can be induced with a phorbol ester, but not with a calcium ionophore. The truncated form of LHR also was down-regulated normally in response to hCG stimulation. In contrast, the cell lines expressing LHR-t63I or LHR-628, the two phosphorylation-negative receptor mutant, showed a delay in the early phase of hCG-induced desensitization, a complete loss of PMA-induced desensitization, and an increase in the rate of hCG-induced receptor down-regulation. These results clearly show that residues 632-653 in the C-terminal tail of the LHR are involved in PMA-induced desensitization, hCG-induced desensitization, and hCG-induced down-regulation. Recently, constitutively activating mutations of the receptor have been identified that are associated with familial male-precocious puberty. Cells expressing LHR-D556Y bind hCG with normal affinity, exhibit a 25-fold increase in basal cAMP and respond to hCG with a normal increase in cAMP accumulation. This mutation enhances the internalization of the free and agonist-occupied receptors ~2- and ~17-fold, respectively. We conclude that the state of activation of the LHR can modulate its basal and/or agonist-stimulated internalization. Since the internalization of hCG is involved in the termination of hCG actions, we suggest that the lack of responsiveness detected in cells expressing LHR-L435R is due to the fast rate of internalization of the bound hCG. This statement is supported by the finding that hCG responsiveness is restored when the cells are lysed and signal transduction is measured in a subcellular fraction (membranes) that cannot internalize the bound hormone.

• Korean Journal of Soil Science and Fertilizer
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• v.28 no.4
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• pp.306-311
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• 1995

A nutrient solution culture experiment was conducted in the greenhouse of Suwon Campus, Kyung-Hee University from May 22 to July 10, 1995, to determine the effects of sulful application on seedling growth of Kwanganok and Hwengsungok cultivars of corn. Plants were harvested at weekly interval for seven weeks, at which time plant height, leaf area and dry matter yield of corn seedling were measured. Sulfer was treated with $MgSO_4{\cdot}7H_2O$ so that sulfur concentrations of cultural solution are 0, 68, 136 and 204ppm for treatments of without sulfer(-S), control(C), twofold concentration(2S) of control and threefold contration(3S) of control, respectively. The plant height, leaf area and dry matter yield during the seven weeks of Kwanganok cultivar was decreased in order of C>2S>3S>-S, 2S>C>3S>-S and 2S>3S>C>-S application pot, respectively. In Hwengsungok cultivar, those were decreased in order of C>-S>3S>2S, C>3S>2S>-S and C>3S>-S>2S application pot, respectively. The total plant height, leaf area and dry matter yield during the whole period of Kwanganok and Hwengsungok cultivars were decreased in the seedling grown under without sulfur treatment compared with the other sulfur treatments. In Hwengsungok cultivar, plant height, leaf area and dry matter yield of seedlings were increased more in control sulfur treatment pot than the other sulfur treatment pot during the whole period. The crop growth rates of seedling of both cultivars in all treatments were slowly increased until the 3th week after planting and its were showed up and down CGR's thereafter, but Hwengsungok cultivar with C treatment pot maintained a steady increase in CGR until the end of the experiment period.

• Korean Journal of Soil Science and Fertilizer
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• v.29 no.1
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• pp.26-33
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• 1996

A nutrient solution culture experiment was conducted in the greenhouse at Suwon Campus. Kyung-Hee University from May 23 to July 11. 1995, to determine the effects of potassium application on seedling growth of Kwanganok and Hwengsungok cultivars of corn. Potassium was treated with four different levels, which are 0.248.496 and 744ppm for treatments without potassium (-k), standard potassium concentration (K), double(2k) and triple (3k) of standard potassium concentration, respectively. Plants were harvested at weekly interval for seven weeks. at which time plant height, leaf area and dry matter yield of corn seedling were measured, only chlorophyll content was taken for five weeks from three week after planting. Seedlings growth of Kwanganok and Hwengsungok cultivars was greatly retarded from planting to harvesting time in 3k treatment. The plant height, leaf area and dry matter yield of both cultivars during the whole period were decreased in order of K>2F>-K>3K, but dry matter yield of Kwanganok cultivar was decreased in order of 2K>K>-K>3K. Chlorophyll content of Kwanganok and Hwengsungok cultivars was irregularly fluctuated during growing period. but generally highest in K treatment and lowest in 3k treatment. The crop growth rates of seedlings of both cultivars were slow until the 3rd week after planting. but very fast thereafter. In general, CGR of both cultivars was higher in K and 2k treatments than -k and 3k treatments.

• Korean Journal of Animal Reproduction
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• v.24 no.4
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• pp.357-364
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• 2000

Members of the glycoprotein family, which includes CG, LH, FSH and TSH, comprise two noncovalently linked $\alpha$- and $\beta$-subunits. Equine chorionic gonadotropin (eCG), known as PMSG, has a number of interesting and unique characteristics since it appears to be a single molecule that possesses both LH- and FSH-like activities in other species than the horse. This dual activity of eCG in heterologous species is of fundamental interest to the study of the structure-function relationships of gonadotropins and their receptors. CG and LH $\beta$ genes are different in primates. In horse, however, a single gene encodes both eCG and eLH $\beta$ -subunits. The subunit mRNA levels seem to be independently regulated and their imbalance may account for differences in the quantities of $\alpha$ - and $\beta$-subunits in the placenta and pituitary. The dual activities of eCG could be separated by removal of the N-linked oligosaccharide on the $\alpha$-subunit Asn 56 or CTP-associated O-linked oligosaccharides. The tethered-eCG was efficiently secreted and showed similar LH-like activity to the dimeric eCG. Interestingly, the FSH-like activity of the tethered-eCG was increased markedly in comparison with the native and wild type eCG. These results also suggest that this molecular can implay particular models of FSH-like activity not LH-like activity in the eCG/indicate that the constructs of tethered molecule will be useful in the study of mutants that affect subunit association and/or secretion. A single-chain analog can also be constructed to include additional hormone-specific bioactive generating potentially efficacious compounds that have only FSH-like activity. The LH/CG receptor (LH/CGR), a membrane glycoprotein that is present on testicular Leydig cells and ovarian theca, granulosa, luteal, and interstitial cells, plays a pivotal role in the regulation of gonadal development and function in males as well as in nonpregnant and pregnant females. The LH/CGR is a member of the family of G protein-coupled receptors and its structure is predicted to of a large extracellular domain connected to a bundle of seven membrane-spanning a-helices. The LH/CGR phosphorylation can be induced with a phorbol ester, but not with a calcium ionophore. The truncated form of LHR also was down-regulated normally in response to hCG stimulation. In contrast, the cell lines expressing LHR-t631 or LHR-628, the two phosphorylation-negative receptor mutant, showed a delay in the early phase of hCG-induced desensitization, a complete loss of PMA-induced desensitization, and an increase in the rate of hCG-induced receptor down-regulation. These results clearly show that residues 632~653 in the C-terminal tail of the LHR are involved in PMA-induced desensitization, hCG-induced desensitization, and hCG-induced down-regulation. Recently, constitutively activating mutations of the receptor have been identified that are associated with familial male-precocious puberty. Cells expressing LHR-D556Y bind hCG with normal affinity, exhibit a 25-fold increase in basal cAMP and respond to hCG with a normal increase in cAMP accumulation. This mutation enhances the internalization of the free and agoinst-occupied receptors ~2- and ~17- fold, respectively. We conclude that the state of activation of the LHR can modulate its basal and/or agonist-stimulated internalization. Since the internalization of hCG is involved in the termination of hCG actions, we suggest that the lack of responsiveness detected in cells expressing LHR-L435R is due to the fast rate of internalization of the bound hCG. This statement is supported by the finding that hCG responsiveness is restored when the cells are lysed and signal transduction is measured in a subcellular fraction (membranes) that cannot internalize the bound hormone.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.37 no.1
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• pp.45-53
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• 1992

This studies were conducted to investigate the physiological characteristics of photosynthesis and dry matter accmulation of 2 Tongil type and 2 Japonica type varieties under different temperature and light intensity condition. Photosynthetic activities were lower in Tongil type varieties than Japonica type at low temperature(17$^{\circ}C$), but higher in Tongil type varieties at high temperature in each growth stages. The degradation rate of photosynthesis was higher in Tongil type varieties than Japonica type varieties at low temperature and Tongil type varieties were showed high photosynthetic activities at high temperature ($25^{\circ}C$). Specific Leaf Area in each growth stages were the highest at tillering and increased from panicle formation stage to heading stage. The ratio of respiration to photosynthesis (R /P$\times$200) into upper three leaves were significantly high in third leaf and showed same slope in each varieties. CGR, NAR were higher in Tongil type varieties than Japonica type varieties and yields, havesting index were showed high in Tongil type varieties.

• Journal of Korean Society of Forest Science
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• v.77 no.4
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• pp.361-370
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• 1988

This study was carried out for observation of growth, mycorrhizal formation and nutrient absorption of Pinus thunbergii seedlings treated with two soil mixtures and various nitrogen levels after inoculation with mycorrhizal fungus, Pisolithus tinctorius. 1. Seedlings grown on vermiculite applied with $50-150{\mu}g/ml$ nitrogen levels were well developed with pinnate type and cluster-like mycorrhizae. But seedlings on sandy loam had monopodial type in addition to the above-mentioned two types. 2. Optimum fertilization level for mycorrhizal formation is 50 or $150{\mu}g/ml$ N that showed best mycorrhizal formation of $86.4({\pm}3.14)%$ or $73.0({\pm}7.21)%$, respectively, but increased nitrogen levels decreased formation of mycorrhizal short roots. Seedlings applied with $450{\mu}g/ml$ nitrogen level decreased in net assimilation rate (NAR) and crop growth rate(CGR) during early growth of the seedlings, and they were increased since Aug, when nutrient application was stopped. 9. Inorganic nutrient absorption was increased more in seedlings grown on vermiculite and inoculated growth medium than those grown on sandy loam and noninoculated one, and it was gradually increased with increasing nitrogen increasing nitrogen level until $350{\mu}g/ml$. But $450{\mu}g/ml$ nitro gen level rather reduced absorption of nutrient.

• Journal of Bio-Environment Control
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• v.7 no.2
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• pp.151-158
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• 1998

This study was carried out to evaluate the effect of substrates and nutrient solution supplying methods in the media culture using perlite and its mixtures with rice hull, carbonized rice hull. cocopeat on the growth and fruit qualify of hydroponically grown cucumber. Three substrates. Perlite(70%) +rice hull(30%) perlite(70%) +carbonized rice hull(30%) and perlite(70%)＋cocopeat(30%) were compared with perlite medium. Supplying methods of nutrient solution were composed of drip irrigation, modified drip irrigation(covered with cheesecloth between drip hose and substrate) and mist system Leaf area of cucumber Plants was lowest in perlite medium while it was highest in mixture of perlite and cocopeat. NAR was higher in the plot of mixture with cocopeat. and same trend was observed in LAI and CGR. T/R ratio was higher in Perlite and perlite mixture with rice hull. Fruit Yields increased in the plot of modified drip irrigation system. When perlite mixture with rice hull used as a substrate. mist system was recommended. Perlite mixture with carbonized rice hull was observed favored in conventional drip irrigation system. Marketable yield and the number of fruit per plant increased and the malformed fruit decreased in the plot of modified drip irrigation system with the mixtures of Perlite and cocopeat.

• Research in Plant Disease
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• v.19 no.4
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• pp.326-330
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• 2013

During the 2012 growing season, 154 leaf samples were collected from sweet cherry trees in Hwaseong, Pyeongtaek, Gyeongju, Kimcheon, Daegu, Yeongju and Eumseong and tested for the presence of Cherry green ring mottle virus (CGRMV). PCR products of the expected size (807 bp) were obtained from 6 samples. The PCR products were cloned and sequenced. The nucleotide sequences of the clones showed over 88% identities to published coat protein sequences of CGRMV isolates in the GenBank database. The sequences of CGRMV isolates, CGR-KO 1-6 shared 98.8 to 99.8% nucleotide and 99.6 to 100% amino acid similarities. Phylogenetic analysis indicated that the Korean CGRMV isolates belong to the group II of CGRMV coat protein genes. The CGRMV infected sweet cherry trees were also tested for Apple chlorotic leaf spot virus (ACLSV), Apple mosaic virus (ApMV), Cherry necrotic rusty mottle virus (CNRMV), Cherry mottle leaf virus (CMLV), Cherry rasp leaf virus (CRLV), Cherry leafroll virus (CLRV), Cherry virus A (CVA), Little cherry virus 1 (LChV1), Prune dwarf virus (PDV) and Prunus necrotic ringspot virus (PNRSV) by RT-PCR. All of the tested trees were also infected with ACLSV.