Mycorrhizal Development and Growth Stimulation of Pinus thunbergii Seedlings Inoculated with Pisolithus tinctorius at Two Soil Mixtures Treated with Six Nitrogen Levels

배양토(培壤土) 및 질소(窒素) 시비(施肥) 수준(水準)이 모래밭 버섯균(菌) (Pisolithus tinctorius)을 접종(接種)한 해송(海松)(Pinus thunbergii) 묘(苗)의 생장(生長)과 균근(菌根) 형성(形成)에 미치는 영향(影響)

This study was carried out for observation of growth, mycorrhizal formation and nutrient absorption of Pinus thunbergii seedlings treated with two soil mixtures and various nitrogen levels after inoculation with mycorrhizal fungus, Pisolithus tinctorius. 1. Seedlings grown on vermiculite applied with $50-150{\mu}g/ml$ nitrogen levels were well developed with pinnate type and cluster-like mycorrhizae. But seedlings on sandy loam had monopodial type in addition to the above-mentioned two types. 2. Optimum fertilization level for mycorrhizal formation is 50 or $150{\mu}g/ml$ N that showed best mycorrhizal formation of $86.4({\pm}3.14)%$ or $73.0({\pm}7.21)%$, respectively, but increased nitrogen levels decreased formation of mycorrhizal short roots. Seedlings applied with $450{\mu}g/ml$ nitrogen level decreased in net assimilation rate (NAR) and crop growth rate(CGR) during early growth of the seedlings, and they were increased since Aug, when nutrient application was stopped. 9. Inorganic nutrient absorption was increased more in seedlings grown on vermiculite and inoculated growth medium than those grown on sandy loam and noninoculated one, and it was gradually increased with increasing nitrogen increasing nitrogen level until $350{\mu}g/ml$. But $450{\mu}g/ml$ nitro gen level rather reduced absorption of nutrient.

본(本) 연구(硏究)는 해송묘목(海松苗木)에 모래밭버섯(Pisolithus tinctorius) 균(菌)을 인공(人工) 접종(接種)한 후(後) 두개의 혼합토(混合土)와 6개의 질소수준(窒素水準)으로 처리(處理)된 실생묘(實生苗)의 생장(生長)과 균근(菌根) 형성(形成) 그리고 양료흡수관계(養料吸收關係)를 추정(推定)키 위(爲)해 실시(實施)되었다. 1) $50-150{\mu}g/ml$ N로 시비(施肥)된 vermiculite에서 자란 실험묘(實生苗)는 pinnate type과 cluster-like type이 잘 발달(發達)하였으나, 사질괴토(砂質壞土)는 monopodial, cluster-like type 그리고 pinnate type 이 발달(發達)하였다. 2) 균근형성(菌根形成)의 과정시비수준(適正施肥水準)은 50 또는 $150{\mu}g/ml$로 균근형성(菌根形成)이 각각(各各) $86.4({\pm}3.14)%$%와 $73.0({\pm}7.21)%$였다. 그러나 질소수준(窒素水準)이 증가(增加)하면 균근형성(菌根形成)이 감소(減少)하였다. 3) $450{\mu}g/ml$N로 시비(施肥)된 실생묘(實生苗)는 순(純) 회수율(同化率)(NAR)과 개체생장율(個體生長率)(CGR)이 생장초기(生長初期)에는 감소(減少)하였으나 질소시비(窒素施肥)가 멈춘 8월(月) 이후(以後)에는 다시 증가(增加)하였다. 4) 실생묘(實生苗)의 무기양료(無機養料) 흡수(吸收)는 질괴토(砂質壞土) 및 무접종구(無接種區)에 비해 vermiculite와 균근처리구(菌根處理區)에서 증가(增加)하였다. 그리고 회수율(回收率)은 $350{\mu}g/ml$ 질소수준(窒素水準)까지는 질소시비(窒素施肥) 증가(增加)에 따라 점진적(漸進的)으로 증가(增加)하였으나 $450{\mu}g/ml$ 질소수준(窒素水準)에서는 도리어 흡수(吸收)가 제한(制限)되었다.