• 생물환경조절학회지
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• 제23권3호
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• pp.250-255
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• 2014

본 연구는 살균 소독제로 오존을 이용하고. 살균효과를 극대화 할 수 있는 방식으로 마이크로버블 장치에 오존을 공급하여 배양액 재처리 기술을 위한 오존 마이크로버블의 살균효과를 구명하고자 실시하였으며 결과를 요약하면 다음과 같다. 마이크로버블 장치의 성능에서 압력 $3.5kgf{\cdot}cm^{-2}$에서 평균입경은 $27.42{\mu}m$로 측정되었고 마이크로버블의 발생량은 당 평균 12만개로 나타났으며, 적정 오존 발생량은 $3g{\cdot}h^{-1}$일 때 오존농도는 2ppm, 배출 오존농도는 0.06ppm에 도달하는 것으로 나타났다. 병원균 살균효과는 오존수의 경우 FO, PC와 CG 모두 오존농도 0.5ppm, 처리시간 30초만으로도 현저한 감소를 보였다. FO는 오존농도 0.5ppm, 처리시간 60초 이내에서 멸균되었고, PC는 오존농도 2.0ppm, 처리시간 30초 이내에서 멸균되었으나, CG의 경우 2.0ppm 이상의 오존수를 처리하여야 할 것으로 판단되었다. 오존가스의 경우 처리시간 120초 이내에서 현저한 감소를 보였으며, FO와 PC는 처리시간 180초 이내에서 멸균되었고, CG의 경우 180초 이상 오존가스를 처리하여야 할 것으로 판단되었다.

• 한국수정란이식학회지
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• 제19권3호
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• pp.257-264
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• 2004

멸종위험이 큰 우리나라 재래돼지를 유전자원으로서 안전하게 보존하고 유전적 다양성을 유지하기 위한 수단으로 수정란을 채취하여 동결보존하기 위해서 미경산 재래돼지에서 과배란 유기를 위한 적정 호르몬의 수준과 수정란의 회수 및 동결보존 방법을 확립하고자 수행한 결과는 다음과 같다. 1. hCG 500IU와 PMSG를 500, 750, 1,000IU 및 hCG 750IU와 PMSG 1,000IU를 각각 투여한 재래돼지의 배란황체와 미배란난포의 수는 각각 12.4, 13.6, 30.0 및 23.3개로 PMSG 1,000IU와 hCG 500IU를 투여한 재래돼지가 다른 용량의 처리돼지보다 난소반응이 양호하였으나 유의적인 차이는 없었으며, 배란황체수에 대한 수정란 회수율은 59.4-79.2％ 수준이었다. 2. 과배란처리된 공란돈에서 수정후 4일에 회수된 수정란의 발육단계는 상실기의 수정란이 수정후 5일보다 유의적으로 많이 회수되었으며(P<0.01), 수정후 5일에 회수된 배 반포기의 수정란을 수정후 4일보다 유의적으로 많이 회수되었다(P<0.05). 3. 확장배반포기 수정란을 1.4M glycerol의 항동 해제를 이용하여 관행의 완만동결법으로 동결한 처리에서 생존율은 25.3％였다.

• 한국가축번식학회지
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• 제19권2호
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• pp.135-140
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• 1995

The study was conducted to investigate on the survival rate of bisected porcine embryos co-cultured in 10% FCS(v/v)＋TCM-199 media containing hormones, oviductal epithelial cells and cumulus cells 0 to 72 hrs after bisection. In vitro survival rate was defined as development rate on in vitro culture or FDA-test. The results are summarized as follows ; 1. The survival rate of bisected porcine embryos cultrued in 10% FCS＋TCM-199 media contaning PMSG, hCG, PMSG＋hCG, hCG＋$\beta$-estradiol 0 to 20 hrs and 20 to 40 hrs were 37.6% and 37.5%, 28.6% and 28.6%, 35.7% and 28.8%, 30.8% and 23.1%, 38.5% and 30.7%, respectively. The survival rate of bisected embryos co-cultured in TCM-199 media containing hormones, oviductal epithelial cells and cumulus cells was significantly higher than that of non co-culture. 2. The survival rate of bisected porcine embryos co-cultured 10% FCS＋TCM-199 media containing oviductal epithelial cells 4 to 5 hrs and 20 to 24 hrs were 42.9% and 38.5%, respectively. 3. The survival rate of bisected porcine embryos co-cultured in 10% FCS＋TCM-199 media containing cumulus cells 4 to 5 hrs and 20 to 24 hrs were 40.0% and 35.7%, respectively.

• Asian-Australasian Journal of Animal Sciences
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• 제28권11호
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• pp.1599-1605
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• 2015

Twenty Holstein calves were used to investigate the effects of mannanoligosaccharides (MOS) supplementation in the whole milk on growth performance, faecal score, faecal pH, selected faecal bacterial populations and health during the preweaning period. Healthy calves selected by clinical examination were allocated to one of the two groups (control [CG] and experimental [EG]) at 5 days old. Each group consisted of 5 male and 5 female calves. Each calf in EG was supplemented with 7 g/d of a MOS product (Celmanax) from 5 days to 56 days of age. MOS supplement was mixed with the whole milk once in the morning and administered to the calves in EG via nipple bottle, whereas the calves in CG were fed the whole milk without MOS. Calves were weaned at 56 days of age. The final body weight, average daily weight gain (ADG) and average daily feed intake (ADFI) were statistically similar (p>0.05) but were higher by 3.70%, 6.66%, and 10.97%, respectively, in MOS than in control calves. Feed efficiency (ADG/ADFI) was also similar in two calves group. While faecal scores did not differ on day 5, 7, 14, 21, 28, 42, 49, and 56 between groups, EG had a higher faecal score (p = 0.05) than CG on day 35. Faecal concentration of Lactobacillus was lower (p<0.05) in EG compared with CG. No differences (p>0.05) in faecal concentrations of Bifidobacterium, Clostridium perfringens, and Escherichia coli were found between groups. Although there were no significant differences (p>0.05) in the incidence of diarrhoea, treatment days for diarrhoea and the costs associated with diarrhoea treatments between groups, collectively, the observed reductions in treatment days and the cost of diarrhoea treatments accompanying increases in final body weight, ADG and ADFI for EG may indicate potential benefit of MOS in treatment of diarrhoea.

• Earthquakes and Structures
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• 제18권2호
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• pp.201-213
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• 2020

Seismic damages that occurred by the effects of epicenter distance of the earthquake are one of the most important problems for the earthquake engineering. In this study, it is aimed to examine the nonlinear seismic behaviors of concrete gravity (CG) dams considering various epicenter distances. For this purpose, Boyabat CG dam that is one of the biggest concrete gravity dams in Turkey is selected as a numerical application. FLAC3D software based on finite difference method is used for modelling and analyzing of the dam. Drucker-Prager nonlinear material model is used for the concrete body and Mohr-Coulomb nonlinear material model is taken into account for the foundation. Special interface elements are used between dam body and foundation to represent interaction condition. Free-field and quiet non-reflecting boundary conditions are utilized for the main surfaces of 3D model. Total 5 various epicenter distances of 1989 Loma Prieta earthquake are considered in 3D earthquake analyses and these distances are 5 km, 11 km, 24 km, 85 km and 93 km, respectively. According to 3D seismic results, x-y-z displacements, principal stresses and shear strain failures of the dam are evaluated in detail. It is clearly seen from this study that the nonlinear seismic behaviors of the CG dams change depending to epicenter distance of the earthquake. Thus, it is clearly recommended in this study that when a CG dam is modelled or analyzed, distance of the earthquake fault to the dam should be strongly examined in detail. Otherwise, earthquake damages can be occurred in the concrete dam body by the effects of seismic loads.

• 한국가축번식학회지
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• 제7권1호
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• pp.19-23
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• 1983

Present studies were conducted to investigate the developmental stage and the location of embryos in the reproductive tract at various times after ovulation, the morphologically normal after thawing of embryos preserved in liquid nitrogen, and the survival after transferring frozen-thawed embryos. The results obtained were as follows: 1. Embryo stage and location in the reproductive tract after hCG administration. For the investigation of embryo stage and location in the reproductive tract after ovulation, rabbits were laparotomized at 24, 40, 48, 72 and 120 hrs post hCG injection, simultaneously with mating. the oviducts and uteri were flushed out with PBS medium containing 50% rabbit serum, respectively. 1) Most of embryos was remained in the oviduct within 48 hrs, with the lapse of time, embryos were started to move to uterus and shifted in uterus at 72 hrs after hCG injection. 2) The representatives of embryos stage collected at 24, 40, 48, 72 and 120 hrs were 1-cell(60.4%), 8-cell to early morula (52.3, 39.3%), late blastocyst (95.5%) stages, respectively. 2. Morphological normality and survival of the frozen-thawed embryos. For the evalution of the quality and viability on the frozen-thawed embryos, immediately after thawing, embryos were assessed by morphologically normal under a dissecting microscope, and a further test of frozen-thawed embryos was made by transferring the morphologically normal embryos to the uteri of recipient rabbit induced pseudopregnancy by the injection of hCG at the time of hCG injection in donor rabbits. 1) The propotions of embryos which a, pp.ared morphologically normal was higher when 8-cell (85.7%) and morula(90.5%) were used for freezing than when 4-cell (66.7%) and blastocyst (75.8%) were used. 2) Preganacies were observed at Day 15 after transfer of frozen-thawed 8-cell (7/13), morula (19/42) and blastocyst (3/19) but not after transfer of embryos at 4-cell stage.

• 조명전기설비학회논문지
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• 제17권4호
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• pp.1-7
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• 2003

본 연구의 목적은 야간경관조명의 다양한 조명기법을 대상으로 컴퓨터 시뮬레이션을 통한 이미지 예측실험을 통해 조명기법별 평가특성을 분석하기 위한 기초연구로서 현장실험을 기준으로 물리량 및 주관 평가의 유효성을 검증하는데 있다. 연구는 우선 예비실험을 통해 선정된 평가어휘를 바탕으로 현장실험의 실험조건별 사진촬영과 물리량 측정 및 주관평가를 수행하여 조명 해석 프로그램에서 계산된 물리량과, 현장 실험에서 측정된 물리량을 비교하였으며 각각의 주관평가 결과를 가지고 현장과 CG, 현장과 사진에 대한 유효성을 검증하였다. 연구결과 1) 물리량의 유효성 검증결과 유의차가 5% 내외로 유의차가 없었으며 2) 현장실험과 CG, 현장실험과 사진실험의 주관평가 유효성 검증 결과 일부 항목을 제외한 대부분의 항목에서 유의차가 없는 것으로 나타났다.

• Asian-Australasian Journal of Animal Sciences
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• 제32권1호
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• pp.137-143
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• 2019

Objective: The aim of this study was to compare male and female geese of two contrasting genotypes in terms of fatty acid composition, indexes related to human health, lipid metabolism and oxidative stability of the meat. Methods: The experiment was carried out on total of 120 geese of two different genotypes; the native breed Czech goose (CG) and commercial hybrid Novohradska goose (NG). One-d-old goslings were divided into 4 groups according to genotype and sex, and 8 birds from each group were slaughtered at 8 weeks of age. Results: The effects of the interactions between genotype and sex were observed on growth performance and carcass traits. Final body weight (p<0.001), daily weight gain (p<0.001), daily feed intake (p<0.001), slaughter weight (p<0.001), and cold carcass weight (p<0.001) were highest in NG males and lowest in CG females. The meat fatty acid composition results showed effects of both genotype and sex on the total n-6 and the total polyunsaturated fatty acid (PUFA) content, as well as the PUFA n-6/PUFA n-3 ratio. Regarding genotype, the total n-6, the total PUFA content and the PUFA n-6/PUFA n-3 ratio were higher in CG, and higher values were found in females. In terms of the lipid metabolism, ${\Delta}^5-{\Delta}^6$ desaturase (p = 0.006) was higher in males. The meat oxidative stability results revealed an interaction between genotype, sex and storage time (p<0.001). The highest (13.85 mg/kg) malondialdehyde content was measured in the meat of CG females after 5 days of storage and was presumably related to a higher PUFA content. Conclusion: NG had a relatively higher growth rate and meat oxidative stability, whereas the advantage of CG meat is its favourable fatty acid profile characterized by a higher PUFA content.

• 한국가축번식학회지
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• 제19권3호
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• pp.227-234
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• 1995

본 연구는 1-세포기배의 glucose에 대한 노출이 상실배기 이후의 배발생에 미치는 영향을 검토하고자 실시되었다. hCG 주사 후 24~25시간 째에, F1hybrid(C57BL/6, ♀ $\times$CBA/N, ♂) 계통 생쥐를 도살하여 1-세포기배를 회수한 후 0.1% hyaluronidase로 처리하여 난구세포를 제거하였다. 1-세포기배는 hCG 주사 후 72시간째 다양한 농도의 glucose(5.5, 16.5, 27.5 및 38.5mM)에 1분 노출 후 glucose가 첨가되어 있거나 혹은 첨가되지 않은 CR1aa배양액에서 계속 배양함으로써 배발생을 유도하였다. 이 실험의 결과를 요약하면 다음과 같다. 1. M2 배양액에서 회수한 후 3mg/ml의 Fatty-acid free BSA가 첨가된 배양액에서 배양한 경우 27.5%의 확장배반포까지의 배발생율과 16.6%의 탈출 배반포까지의 배발생율을 나타낸 반면, TL HEPES 배양액에서 회수한 경우는 전혀 상실배기 이후의 배발생이 나타나지 않았다. 2. hCG 주사 후 72시간째에 단 1분간의 27.5mM glucose에 대한 노출만으로도 68.8% (CR1aa＋BSA)와 77.1%(CR1aa＋FBS)의 확장배반포까지의 발생을 유도할 수 있었다. 그러나 1분 노출과 이후 계속되는 노출간에는 배발생에 있어서 유의차는 인정되지 않았다. 3. hCG 주사 후 72시간째에 5.5, 16.5, 27.5 및 38.5mM의 glucose 첨가에 따른 확장 배반포까지의 배발생율은 45.7~61.5%로 각 처리군의 유의차는 없었으며, 따라서 고농도의 glucose 첨가에 따른 저해효과는 확인할 수 없었다.

• Journal of Applied Biological Chemistry
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• 제64권4호
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• pp.453-463
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• 2021

5년근 이상의 인삼(CG), 산양삼(MCG) 및 산양삼 전초(WPMCG)의 지방산, 아미노산, 무기질, ginsenosides, 총 phenolics, 총 flavonoids 및 항산화 활성을 비교하여 분석하였다. 총 지방산 및 불포화 지방산 함량은 MCG (1215.9 및 751.2 mg/100 g)에서, 포화 지방산은 WPMCG (486.4 mg/100 g)에서 각각 높았으며 α-linolenic acid 함량은 특징적으로 CG (18.1 mg/100 g)에 비해 MCG (139.3 mg/100 g) 및 WPMCG (194.4 mg/100 g)에서 최대 10배 높게 검출되었다. 총 유리아미노산 및 필수 아미노산 함량은 CG < MCG < WPMCG (1006.35 및 839.46 mg/100 g) 순으로 높았으며, γ-aminobutyric acid 및 arginine이 주된 비필수 아미노산으로 각각 WPMCG (163.10 mg/100 g)와 MCG (305.23 mg/100 g)에서 가장 높게 검출되었다. 총 무기질 함량은 CG (30.36 mg/100 g)와 WPMCG (29.82 mg/100 g)에서 높게 검출되었으며, 칼슘(Ca)은 CG 및 MCG와 비교하여 WPMCG (6.68 mg/100 g)에서 약 2배 이상의 높게 검출되었다. 총 phenolics (5.12 GAE mg/g), 총 flavonoids (3.04 RE mg/g) 및 ginsenosides (42.44 mg/g) 함량은 WPMCG에서 공통적으로 가장 높은 함량이 검출되었으며 이와 유의적으로 항산화 활성 역시 WPMCG에서 가장 높은 활성을 나타내었다.