• KCI Concrete Journal
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• 제12권1호
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• pp.69-77
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• 2000

The reinforced concrete(RC) flexural members strengthened with steel plate/CFS at soffit have initial stresses and strains in reinforcements and concrete caused by the service loads at the time of retrofitting works. These initial residual stresses and strains of strengthened beams may affect the flexural performance of the rehabilitated beams. The objective of this study is to evaluate and verify the effectiveness of rehabilitation by external bonded steel plates and CFS to the tension face of the beams under three conditions of pre-loading. Thirteen beam specimens are tested and analyzed. Main test parameters are pre-loading conditions, strengthening materials and reinforcement ratio of specimens. The effect of test parameters on the strengthened beams is analyzed from the maximum load capacity, load-deflection relationship, state of stress of the materials. crack propagation phase, and failure modes. Both test results and design formulas of ACI Code provisions are compared and evaluated.

• 한국농촌건축학회논문집
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• 제6권3호
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• pp.106-115
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• 2004

In this paper, the compressive strength and ductility enhancement of concrete by lateral confinement of carbon-fiber sheets(CFS) have been studied experimentaly with cylinder specimens and square short columns reinforced externally by CFS. Test variables were amount of lateral reinforcement by CFS and space of hoop bars. Test results showed that lateral reinforcements by carbon-fiber sheets provided lateral confinement successfully for the concrete specimens and were more effective for ductility enhancement than for strength increase, and that the lateral confinement coefficient of cabon-fiber sheets increased according to narrowing the space of hoop bars in the double lateral confinement made by CFS and hoop bars.

• 대한한의학회지
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• 제34권3호
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• pp.119-125
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• 2013

Objectives: Medically unexplained fatigue includes chronic fatigue syndrome (CFS) and idiopathic chronic fatigue (ICF). These disorders quite impair quality of life while no effective therapies exist. Therefore, patients with CFS or ICF frequently choose treatments based on traditional Korean medicine. The study aimed to analyze characteristic of patients visiting an Oriental clinic with CFS or ICF. Methods: Patients who met criteria for CFS or ICF were enrolled in this study, from March 2011 to Jun 2013. Clinical information focused on symptom differentiation and Sasang constitution was analyzed. Pearson's chi-square test was used to compare the frequency among sub-groups. Results: In total, 77 patients (49 male and 28 female) were diagnosed with CFS (17 male and 13 female) or ICF (32 male and 15 female). The symptom differentiation was 37.7%, 26.0%, 13.0%, and 23.3% for "Qi deficiency of both spleen and lung (脾肺氣虛)", "Yin deficiency of both liver and kidney (肝腎陰虛)", "Blood deficiency of both heart and spleen (心脾血虛)" and "Yang deficiency of both spleen and kidney (脾腎陽虛)", while Sasang constitutional distribution was 59.7%, 22.1% and 18.2% Soeumin, Taeumin and Soyangin, respectively. The fatigue severity was notably different between CFS ($63.7{\pm}12.1$) or ICF ($52.2{\pm}15.3$) according to the Chalder fatigue scale (10-point scale for eleven questions). There was statistical significance regarding symptom differentiation and Sasang constitution (p < 0.05). Conclusions: In the process of traditional Korean medicine-based development of therapeutics for patients with chronic fatigue or related conditions, this report can serve as reference data.

• 한국결정성장학회지
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• 제25권6호
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• pp.290-293
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• 2015

최근 사회기반시설물의 구조물 유지관리 분야의 관심이 높아짐에 따라 새로운 유지관리 공법 및 신소재에 관한 연구가 활발히 진행되어지고 있다. 그중에서도 유지관리의 주요 대상인 교량 유지관리에는 탄소섬유를 이용한 공법이 주목을 받고 있다. 탄소섬유시트(Carbon Fiber Sheet, 이하 CFS)는 이미 여러 연구자들이 국내에서도 재료시험 및 보강공법에 관한 연구를 진행하여 연구결과가 보고되고 있지만, 탄소섬유 스트랜드 시트(Carbon Fiber Strand Sheet, 이하 CFSS)는 최근 새롭게 개발된 재료로써 아직까지 국내에는 연구 성과가 없는 실정이다. 따라서, 본 연구에서는 CFSS를 RC 상판 공시체에 접착보강하여 내피로성을 평가하였다. 그 결과, 무보강 RC 상판 공시체에 비해 약 25.3배의 보강 효과가 확인되었고, CFS 접착보강 RC 상판 공시체에 비교하여 약 1.2배의 보강 효과가 확인되었다.

• 한국버섯학회지
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• 제14권4호
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• pp.162-167
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• 2016

Lactic acid bacteria (LAB) producing phenyllactic acid (PLA), which is known as antimicrobial compound, was isolated from button mushroom bed and the isolated LAB was identified to Lactobacillus casei by 16 rRNA gene sequence analysis. Cell-free supernatant (CFS) from L. casei was assessed for both the capability to produce the antimicrobial compound PLA and the antifungal activity against three fungal pathogens (Rhizoctonia solani, Botrytis cinerea, and Collectotricum aculatum). PLA concentration was investigated to be 3.23 mM in CFS when L. casei was grown in MRS broth containing 5 mM phenylpyruvic acid as precursor for 16 h. Antifungal activity demonstrated that all fungal pathogens were sensitive to 5% CFS (v/v) of L. casei with average growth inhibitions ranging from 34.58% to 65.15% (p < 0.005), in which R. solani was the most sensitive to 65.15% and followed by C. aculatum, and B. cinerea. The minimum inhibitory concentration (MIC) for commercial PLA was also investigated to show the same trend in the range of 0.35 mg mL-1 (2.11 mM) to 0.7 mg mL-1 (4.21 mM) at pH 4.0. The inhibition ability of CFS against the pathogens were not affected by the heating or protease treatment. However, pH modification in CFS to 6.5 resulted in an extreme reduction in their antifungal activity. These results may indicate that antifungal activities in CFS was caused by acidic compounds like PLA or organic acids rather than protein or peptide molecules.

• Structural Engineering and Mechanics
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• 제58권2호
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• pp.277-300
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• 2016

Cold-formed steel (CFS) sections are becoming an increasingly popular solution for constructing floors in residential, healthcare and education buildings. Their reduced weight, however, makes them prone to excessive vibrations, increasing the need for accurate prediction of CFS floor modal properties. By combining experimental modal analysis of a full-scale CFS framed building and its floors and their numerical finite element (FE) modelling this paper demonstrates that the existing methods (based on the best engineering judgement) for predicting CFS floor modal properties are unreliable. They can yield over 40% difference between the predicted and measured natural frequencies for important modes of vibration. This is because the methods were adopted from other floor types (e.g., timber or standard steel-concrete composite floors) and do not take into account specific features of CFS floors. Using the adjusted and then updated FE model, featuring semi-rigid connections led to markedly improved results. The first four measured and calculated CFS floor natural frequencies matched exactly and all relevant modal assurance criterion (MAC) values were above 90%. The introduction of flexible supports and more realistic modelling of the floor boundary conditions, as well as non-structural $fa{\c{c}}ade$ walls, proved to be crucial in the development of the new more successful modelling strategy. The process used to develop 10 identified and experimentally verified FE modelling parameters is based on published information and parameter adjustment resulting from FE model updating. This can be utilised for future design of similar lightweight steel floors in prefabricated buildings when checking their vibration serviceability, likely to be their governing design criterion.

• Steel and Composite Structures
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• 제33권4호
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• pp.595-614
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• 2019

In cold-formed steel (CFS) structures, such as trusses, transmission towers and portal frames, the use of back-to-back built-up CFS unequal angle sections are becoming increasingly popular. In such an arrangement, intermediate welds or screw fasteners are required at discrete points along the length, preventing the angle sections from buckling independently. Limited research is available in the literature on axial strength of back-to-back built-up CFS unequal angle sections. The issue is addressed herein. This paper presents an experimental investigation on both the welded and screw fastened back-to-back built-up CFS unequal angle sections under axial compression. The load-axial shortening and the load verses lateral displacement behaviour along with the deformed shapes at failure are reported. A nonlinear finite element (FE) model was then developed, which includes material non-linearity, geometric imperfections and modelling of intermediate fasteners. The FE model was validated against the experimental test results, which showed good agreement, both in terms of failure loads and deformed shapes at failure. The validated FE model was then used for the purpose of a parametric study to investigate the effect of different thicknesses, lengths and, yield stresses of steel on axial strength of back-to-back built-up CFS unequal angle sections. Five different thicknesses and seven different lengths (stub to slender columns) with two different yield stresses were investigated in the parametric study. Axial strengths obtained from the experimental tests and FE analyses were used to assess the performance of the current design guidelines as per the Direct Strength Method (DSM); obtained comparisons show that the current DSM is conservative by only 7% on average, while predicting the axial strengths of back-to-back built-up CFS unequal angle sections.

• Steel and Composite Structures
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• 제10권2호
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• pp.187-205
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• 2010

The existing CFT columns present the deterioration in confining effect after the yield of steel tube, local buckling and the deterioration in load capacity. If lateral load such as earthquake load is applied to CFT columns, strong shearing force and moment are generated at the lower part of the columns and local buckling appears at the column. In this study, axial compression test and beam-column test were conducted for existing CFT square column specimens and those reinforced with carbon fiber sheets (CFS). The variables for axial compression test were width-thickness ratio and the number of CFS layers and those for beamcolumn test were concrete strength and the number of CFS layers. The results of the compression test showed that local buckling was delayed and maximum load capacity improved slightly as the number of layers increased. The specimens' ductility capacity improved due to the additional confinement by carbon fiber sheets which delayed local buckling. In the beam-column test, maximum load capacity improved slightly as the number of CFS layers increased. However, ductility capacity improved greatly as the increased number of CFS layers delayed the local buckling at the lower part of the columns. It was observed that the CFT structure reinforced with carbon fiber sheets controlled the local buckling at columns and thus improved seismic performance. Consequently, it was deduced that the confinement of CFT columns by carbon fiber sheets suggested in this study would be widely used for reinforcing CFT columns.

• Journal of Dairy Science and Biotechnology
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• 제39권3호
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• pp.121-127
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• 2021

Yogurt is produced by bacterial fermentation of milk and contains lactic acid bacteria (LAB), which produce various metabolites such as organic acid, hydrogen peroxide, and bacteriocin. This study aimed to investigate cell-free supernatants (CFS) of LAB isolated from Tibetan yogurt. CFS (TY1, TY2, TY3, TY4, TY5, TY6, and TY7) from selected strains of LAB were co-incubated with four different foodborne pathogenic bacteria, namely E. coli O157:H7, Listeria monocytogenes, Salmonella typhimurium, and Staphylococcus aureus. Inhibition of foodborne pathogenic bacterial growth was not affected in the presence of CFS (pH 6.5). In contrast, CFS without neutralization completely inhibited the growth of the bacteria. Furthermore, when the concentration of CFS (without neutralization) was changed to 1:4 and 1:8, a difference in inhibition was observed between Gram-positive and Gram-negative bacteria. CFS more effectively inhibited the growth of Gram-negative E. coli O157:H7 and S. Typhimurium than Gram-positive L. monocytogenes and S. aureus. These results suggest that organic acids in LAB may inhibit the growth of foodborne pathogenic bacteria, particularly Gram-negative bacteria.

• Journal of Microbiology and Biotechnology
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• 제31권7호
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• pp.990-998
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• 2021

Melanin is a natural skin pigment produced by specialized cells called melanocytes via a multistage biochemical pathway known as melanogenesis, involving the oxidation and polymerization of tyrosine. Melanogenesis is initiated upon exposure to ultraviolet (UV) radiation, causing the skin to darken, which protects skin cells from UVB radiation damage. However, the abnormal accumulation of melanin may lead to the development of certain skin diseases, including skin cancer. In this study, the antioxidant and antimelanogenic activities of the cell-free supernatant (CFS) of twenty strains were evaluated. Based on the results of 60% 2,2-diphenyl-1-picrylhydrazyl scavenging activity, 21% 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) scavenging capacity, and a 50% ascorbic acid equivalent ferric reducing antioxidant power value, Limosilactobacillus fermentum JNU532 was selected as the strain with the highest antioxidant potential. No cytotoxicity was observed in cells treated with the CFS of L. fermentum JNU532. Tyrosinase activity was reduced by 16.7% in CFS-treated B16F10 cells (but not in the cell-free system), with >23.2% reduction in melanin content upon treatment with the L. fermentum JNU532-derived CFS. The inhibitory effect of the L. fermentum JNU532-derived CFS on B16F10 cell melanogenesis pathways was investigated using quantitative reverse transcription polymerase chain reaction and western blotting. The inhibitory effects of the L. fermentum JNU532-derived CFS were mediated by inhibiting the transcription of TYR, TRP-1, TRP-2, and MITF and the protein expression of TYR, TRP-1, TRP-2, and MITF. Therefore, L. fermentum JNU532 may be considered a potentially useful, natural depigmentation agent.