• Journal of the Korean Society for Library and Information Science
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• v.37 no.2
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• pp.169-186
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• 2003

Most of the university libraries in Korea provide digital reference service(DRS), but the use of DRS is very low. The low usage of DRS seems to result from the lack of subject librarians, of staff, of public relations, and of managers' enthusiastic attitude and understanding of DRS. It is difficult to solve these problems because of the poor circumstances in universities. The collaborative digital reference service(CDRS) is a solution to these problems. Not only CDRS costs little but also can provide reference services in many different subject areas. Though subject specialist librarian, user-friendly system interface, system programs and administrative supports are necessary to implement CDRS, the education and training of the subject specialist librarians is the most important work to be done in the university library situation in Korea.

• Proceedings of the Korea Technology Innovation Society Conference
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• 2003.05a
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• pp.363-368
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• 2003

• Proceedings of the Korea Society for Energy Engineering kosee Conference
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• 2004.05a
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• pp.319-324
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• 2004

• Journal of the Korean Society for information Management
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• v.21 no.2
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• pp.69-87
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• 2004

This study examines basic concepts and characteristics of QuestionPoint, the typical model of Collaborative Digital Reference Service and Global Reference Network, and analyzes the case of Question포인트 service at Korea Institute of Science and Technology Information where the service is initiated first time in Korea by developing Korean interface. 114 reference questions were analyzed, and questionnaires were sent to 63 service users to find out their overall attitudes and degree of satisfaction. Some suggestions were made by discussing the necessity of the enhancement of CDRS and mutual cooperation among Korean libraries.

• Journal of the Korean Society for information Management
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• v.19 no.4
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• pp.214-231
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• 2002

This paper describes recent developments and trends of digital reference services. A brief overview of selected research on issues surrounding digital reference service is provided. It also discusses how technological developments in libraries have led to the emergence of new service paradigms. This paper briefly reviews reference and information services provided by libraries, those from publishers, those provided by experts, and those where users need to conduct a search and find information through the web. The current trend shows that the cooperative model is increasingly popular. Some projects on collaborative digital reference services(CDRS) are introduced. Librarians will need to continue analyzing the need for cooperative work in the reference service in a digital environment.

• The KIPS Transactions:PartC
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• v.16C no.1
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• pp.51-56
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• 2009

Time synchronization algorithm in wireless sensor networks is essential to various applications such as object tracking, data encryption, duplicate detection, and precise TDMA scheduling. This paper describes CDRS that is a time synchronization algorithm using the Clock Drift rate and Reference Signals between two sensor nodes. CDRS is composed of two steps. At first step, the time correction is calculated using offset and the clock drift rate between the two nodes based on the LTS method. Two nodes become a synchronized state and the time variance can be compensated by the clock drift rate. At second step, the synchronization node transmits reference signals periodically. This reference signals are used to calculate the time difference between nodes. When this value exceeds the maximum error tolerance, the first step is performed again for resynchronization. The simulation results on the performance analysis show that the time accuracy of the proposed algorithm is improved, and the energy consumption is reduced 2.5 times compared to the time synchronization algorithm with only LTS, because CDRS reduces the number of message about 50% compared to LTS and reference signals do not use the data space for timestamp.

• Proceedings of the Korea Society for Energy Engineering kosee Conference
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• 2003.05a
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• pp.551-556
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• 2003

이산화탄소를 줄이기 위한 대책으로 국가 간 기후변화협약이 1992년 6월에 유엔환경개발회의에서 채택되었고 그 후속조치로 1997년 12월 교토에서 OECD 국가들에 대한 선진국 의무를 강화하는 교토의전서가 채택되기에 이르렀다. 국내의 의무부담 적용이 예상되는 2012년에는 국내 이산화탄소 예상 발생량 189백만 탄소톤 중 상당량의 탄소톤을 감축해야 할 것으로 추정된다.(중략)

• The KIPS Transactions:PartC
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• v.8C no.5
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• pp.657-666
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• 2001

In this paper, it is proposed the management items for voice traffic using CDRs so that global carriers can treat and manage the voice traffic for a customer, and defined computational expressions to produce the management items. From them, we have designed the management system, which is composed of web interface module, analysis module, data collection module and database management module, and have improved the availability and convenience of the system using web technologies. In addition, we have tested these items using CDRs in real environments that are collected by the global carrier in order to verify their validity. It is expected that the proposed web based voice traffic management system provide a global carrier with network information collection, fault detection/trouble-shooting and high quality of service through analyzing the characteristics of subscribers.

• IMMUNE NETWORK
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• v.12 no.4
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• pp.155-164
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• 2012

It is well established that blocking the interaction of EGFR with growth factors leads to the arrest of tumor growth, resulting in tumor cell death. ER414 is a human monoclonal antibody (mAb) derived by guided selection of the mouse mAb A13. The ER414 exhibited a ~17-fold lower affinity and, as a result, lower efficacy of inhibition of the EGF-mediated tyrosine phosphorylation of EGFR when compared with mAb A13 and cetuximab. We performed a stepwise in vitro affinity maturation to improve the affinity of ER414. We obtained a 3D model of ER414 to identify the amino acids in the CDRs that needed to be mutated. Clones were selected from the phage library with randomized amino acids in the CDRs and substitution of amino acids in the HCDR3 and LCDR1 of ER414 led to improved affinity. A clone, H3-14, with a ~20-fold increased affinity, was selected from the HCDR3 randomized library. Then three clones, ER2, ER78 and ER79, were selected from the LCDR1 randomized library based on the H3-14 but did not show further increased affinities compared to that of H3-14. Of the three, ER2 was chosen for further characterization due to its better expression than others. We successfully performed affinity maturation of ER414 and obtained antibodies with a similar affinity as cetuximab. And antibody from an affinity maturation inhibits the EGF-mediated tyrosine phosphorylation of EGFR in a manner similar to cetuximab.

• Journal of Microbiology and Biotechnology
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• v.24 no.3
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• pp.408-420
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• 2014

Yeast surface-displayed antibody libraries provide an efficient and quantitative screening resource for given antigens, but suffer from typically modest library sizes owing to low yeast transformation efficiency. Yeast mating is an attractive method for overcoming the limit of yeast transformation to construct a large, combinatorial antibody library, but the optimal conditions have not been reported. Here, we report a large synthetic human Fab (antigen binding fragment) yeast surface-displayed library generated by stepwise optimization of yeast mating conditions. We first constructed HC (heavy chain) and LC (light chain) libraries, where all of the six CDRs (complementarity-determining regions) of the variable domains were diversified mimicking the human germline antibody repertoires by degenerate codons, onto single frameworks of VH3-23 and $V{\kappa}1$-16 germline sequences, in two haploid cells of opposite mating types. Yeast mating conditions were optimized in the order of cell density, media pH, and cell growth phase, yielding a mating efficiency of ~58% between the two haploid cells carrying HC and LC libraries. We constructed two combinatorial Fab libraries with CDR-H3 of 9 or 11 residues in length with colony diversities of more than $10^9$ by one round of yeast mating between the two haploid HC and LC libraries, with modest diversity sizes of ${\sim}10^7$. The synthetic human Fab yeast-displayed libraries exhibited relative amino acid compositions in each position of the six CDRs that were very similar to those of the designed repertoires, suggesting that they are a promising source for human Fab antibody screening.