• Applied Microscopy
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• v.19 no.2
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• pp.59-73
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• 1989

To investigate the effect of cadmium and cadmium binding protein on the electron transport system and conformational changes of rat kidney mitochondria, various cadmium concentration were treated in vitro and respiration rate, NADH-CoQ reductase activity were measured. Ultrastructural changes at state IV respiration were also observed. CdBP was isolated from the rat liver by Sephadex G-75 column fractionation and treated in vitro with cadmium. Also mitochondrial state IV respiration rate was measured. When cadmium was treated in vitro, state IV respiration and enzyme activity were decreased and ultrastructural transformation of mitochondria from a condensed to an orthodox conformation was inhibited under state IV respiration. In case cadmium and CdBP were treated together, oxygen consumption was more increased than cadmium only. Conformational changes of mitochondria from a condensed to orthodox conformation were also observed. This indicates that CdBP have a protective effect against cadmium toxicity.

• YAKHAK HOEJI
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• v.16 no.2
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• pp.73-84
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• 1972

단백질과 전이금속 이온들과의 상호작용을 연구하는데 있어서 단백질중의 이미다졸기 및 아미노산의 반응성은 중요하다. Cd(II)-2-메틸이미다졸 착물들의 경우 이미다졸 분자중의 결합측은 "피로올"형 보다 오히려 "피리딘" 질소임을 알 수 있다. 이 착물들의 환원파는 가역적이며 2전자반응으로 나타난다. 그리고 이미다졸 존재하의 Ni(II) 이온은 촉매파를 나타내며 Ni(II) 이온만의 환원파는 불가역성인 것에 비해 가역성을 보여주었다. 또한 그 반파전위는 Ni(II) 이온만의 반파전위보다 수백 mV 양전위로 이동된다. 이러한 성질은 전극 반응에 관여한 주물질이 전극 표면에서 흡착되어 환원되기 때문이다. 이미다졸 농도의 대수값에 대해 반파전위를 도시했을 때 그 기울기가 30mV로써 이론치와 일치한다. 한편 $Cd(II)-2CH_{3}Im$의 단계적 생성항수 $pK_{1},pK_{2},pK_{3},pK_{4}$는 각각 2.68, 4.01, 4.90, 5.36이었다.4.01, 4.90, 5.36이었다.

• Journal of the Korean Society of Food Culture
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• v.17 no.4
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• pp.456-464
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• 2002

This study was designed to investigates the effects of Korean pueraris radix water extract in Cd(cadmium) administered rats. Forty male Sprague-Dawley rats weighing $100{\pm}10g$ were used for this experiment and divided into following 4 groups; control group, 3% pueraria radix in water extract group, 50 ppm Cd group, 50ppm Cd group with 3% pueraria radix in water extract group. The Cd administered rats were given 50 ppm of $CdCl_2\;{\cdot}\;2H_2O$ disolved in the distilled water. The Cd content in the rats tissue of Cd administered group was lower than in the rats tissue of Cd group with 3% pueraria radix in water extract group. Plasma levels of renin activity was increased by Cd administration group, compared with 3% pueraria radix in water extract group and Cd administred group. Glutamate oxaloacetate transaminase(GOT) and Glutamate pyruvate transaminase(GPT) were increased in Cd-administered group and lower in the 3% extracts of pueraria radix in water extract group. Lactate dehydrogenase(LDHase) was lower in the 3% extracts of pueraria radix-Cd group than in the Cd group. This results suggested that pueraria radix in water extract group, has a lowering effects on the accumulation of Cd and it is belived that the pueraria radix in water extract group has some protective effects to Cd administered in rats, but the mechanism of these effects was obscure.

• Biomedical Science Letters
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• v.18 no.2
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• pp.131-138
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• 2012

Autologous peripheral blood stem cell transplantation (PBSCT) has been used as a major treatment strategy for hematological malignancies. The number of CD34 positive cells in the harvested product is a very important factor for achieving successful transplantation. We studied the factors that can predict the number of CD34 positive cells in the harvested product of acute myelocytic leukemia (AML), multiple myeloma (MM) and Non-Hodgkin's lymphoma (NHL) patients after mobilizing them with chemotherapy plus G-CSF. A total of 73 patients (AML 19 patients, MM 28 patients, NHL 26 patients) with hematological malignancies had been mobilized with chemotherapy and granulocyte colony-stimulating growth factor from April, 2000 to February, 2012. Group's characteristics, checkup opinion of pre-peripheral blood on the day of harvest & outcome of PBSC were analyzed and evaluated using SPSS statistics program after grouping patients as below; group 1: CD34 cell counts < $2{\times}10^6/kg$ (n=16); group 2: $2{\times}10^6/kg{\leq}CD34$ cell counts < $6{\times}10^6/kg$ (n=32); group 3: CD34 cell counts ${\geq}6{\times}10^6/kg$ (n=25). We analyzed the clinical characteristics, the peripheral blood (PB) parameters and the number of CD34 positive cells in the PB and their correlation with the yield of CD34 positive cells collected from the mobilized patients. The total number of leukapheresis sessions was 263 (mean: 3.55 session per patient), and the mean number of harvested CD34 positive cells per patient was $7.37{\times}10^6/kg$. The number of CD34 positive cells in product was significantly correlated with the number of platelet and CD34 positive cells in peripheral blood (P<0.05). The number of PB CD34 positive cells was the best significant factor for the quantity of harvested CD34 positive cells on the linear regression analysis (P<0.05). Many factors could influence the mobilization of peripheral blood stem cells. Platelet count and PB CD34 positive cells count were the two variables which remained to be significant in multivariate analysis. Therefore, the number of platelet and CD34 positive cells in peripheral blood on the day of harvest can be used as an accurate predictor for successful peripheral blood stem cell collection.

• Journal of Ecology and Environment
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• v.30 no.1
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• pp.81-86
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• 2007

Human activities have enhanced the influx of heavy metals to aquatic ecosystem and hanged the abiotic environment such as the sediments supporting benthic organisms. The levels of heavy metals (Cd, Cr, Cu, Ni, Pb and Zn) in the benthic gastropod Oxyloma hirasei and the sediments around their habitat were investigated to examine heavy metal levels and the potential of the gastropod as a bioindicator. We detected different levels of heavy metals in the sediments of two wetland areas, Upo and Mokpo, in the relatively well-conserved Upo wetland, Ramsar Convention Area. Oxyloma hirasei had higher concentrations of heavy metals except Cr and Ni in their soft tissues than in their shells (Cd: $2.10{\sim}3.16$, Cu: $19.73{\sim}28.66$, Pb: $0.67{\sim}1.17$, Zn: $216.1{\sim}285.7\;{\mu}g/g$ dry weight in the soft tissues; Cr: $1.19{\sim}2.58$, Ni: $0.47{\sim}1.16\;{\mu}g/g$ dry weight in the shells). Differences in the Cd, Cu, Ni and Pb concentrations in O. hirasei soft tissues reflected differences in heavy metal concentrations in the sediments at the sampling sites. The coefficients of variation for Cd, Cu and Pb were lower than those for other metals in the soft tissues. Levels of Cd in the tissues of O. hirasei were the highest among the metals examined in this study. Therefore, the soft tissue of O. hirasei appears to be a promising bioindicator particularly for Cd.

• Journal of Korean Dental Science
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• v.11 no.2
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• pp.62-70
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• 2018

Purpose: Bone marrow has long been a source of primary cells. This study was performed to evaluate the effects of age and sex on the cellular viability and expression of stem cell markers of mRNA and on the protein expression of bone marrow stem cells (BMSCs) derived from healthy donors. Materials and Methods: Stem cells were isolated from human bone marrow and plated in culture plates. The shape of the BMSCs was observed under inverted microscope. Quantitative cellular viability was evaluated using a Cell-Counting Kit-8 assay. The expression of stem cell surface markers was tested and a series of quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot was performed to evaluate the expression in each group. Result: The shapes of the cells at 20s, 30s, and 50s were similar to each other. No significant changes in cellular viability were noted among different age groups or sex groups. The BMSCs expressed CD44, CD73, and CD90 surface markers but did not express CD14 and CD34. There were no noticeable differences in CD surface markers among the different age groups. The expressions of CD surface markers were similar between men and women. No significant differences in the secretion of vascular endothelial growth factors (VEGFs) were noted at Day 3 between different age groups. qRT-PCR regarding the expression showed differences between the age groups. However, Western blot analysis showed a decrease in expression but did not reach statistical significance (P>0.05). Conclusion: This study clearly showed no significant differences in shape, cell viability, expression of stem cell surface markers, or secretion of human VEGF among different age groups. However, western blot analysis showed a tendency of age-related decrease which did not reach statistical significance. Collectively, autologous or allogeneic BMSCs should be meticulously applied to obtain optimal results regarding age and sex.

• The Transactions of the Korean Institute of Electrical Engineers C
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• v.48 no.5
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• pp.296-302
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• 1999

$CdIn_2S_4 and CdIn_2S_4 : Co^{2+}$ singlecrystals of thenormal spinel structure were grown by the C.T.R. method. The optical energy band structure of these compounds had a indirect band gap at the fundamental optical absorption band edge. The direct and the indirect energy gaps are found to be 2.325 and2.179eV for $Cdln_2S_4$ , and 2.303 and 2.169eV for $CdIn_2S_4 and CdIn_2S_4 : Co^{2+}$ at 5K, respectivly. The fundamental absorption band edge of these single crystals shift to a shorter wavelength region with decreasing temperature, and the temperature dependence of the optical energy gaps in these compounds satisfy Varshni equation. The Varshni constants$\alpha and \beta$ of the direct energy gap are given by $13.39{\times}10_{-4}eV/K$ and 509 K for $Cdln_2S_4$ and $29.73{\times}10_{-4} eV/K$ and 1398K for $CdIn_2S_4 and CdIn_2S_4 : Co^{2+}$. The Varshni constants ${\alpha}and {\beta}$ of the indirect energy gap are given by 9.68${\times}10^{-4}$ eV/K 308K for $Cdln_2S_4$ and $13.33{\times}10_{-4}eV/K$ and 440K for $CdIn_2S_4 : Co^{2+}$ respectivly. The impurity optical absorption peaks due to cobalt dopant are observed in $CdIn_2S_4 : Co^{2+}$ single crystal. These impurity optical absorption peaks can be attributed to the electronic transitions between the split energy levels of $Co_{2+}$ ions located at $T_d$ symmetry site of $Cdln_2S_4$ host lattece.

• Journal of Soil and Groundwater Environment
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• v.8 no.4
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• pp.68-73
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• 2003

This study performed from 2002 October to 2003 April. The samples were taken at 66 sites, divided into the 6 sections functionally in the southern part of Seoul such as Yangchon-gu, Kangseo-gu, Kuro-gu, Yeongdengpo-gu, Kwanak-gu, Dongjak-gu, Seocho-gu, Kangnam-gu, Songpa-gu, and Kangdong-gu. The result of research showed that each property soil was pH 4.7∼9.5, Cd; 0.391 mg/kg (0.011∼l.081 mg/kg). Cu; 12.35 mg/kg (0.061∼73.62 mg/kg), Pb; 13.04 mg/kg (N.D.∼61.85 mg/kg), Hg; 0.0866 mg/kg (N.D.∼1.353 mg/kg), F; 206.8 mg/kg(47.1∼561.1mg/kg). The minimum and maximum of the concentration with functional soils were Cd; 0.632 mg/kg for multi-purposed soil,0.079 mg/kg for schools, Cu; 21.35 mg/kg for roadside, 2.159 mg/kg for schools, Pb; 24.70 mg/kg for roadside, 1.030 mg/kg for schools, Hg; 0.1780 mg/kg for multi purposed, 0.0087 mg/kg for schools. Especially F was the high concentration at the hills. Also the concentration of Cd, Cu, Pb, Hg and F at school Zone was detected such as low. area of schools area was detected the low concentrations as the items of Cd, Cu, Pb, Hg, and F. The average concentrations of metals and fluorine in the survey area were below the Preliminary Standard of the Soil Preservation Acts in Korea. To evaluate the soil quality of these area showed as a good qualified results. The results of SQPI (Soil Quality Pollution Index the qualified results as much as 86.4%.

• Journal of Environmental Health Sciences
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• v.14 no.1
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• pp.73-86
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• 1988

In order to determine the content levels of trace metals in common salts, 64 bay salt samples were collected from three producing districts and 33 bay salt samples, 32 remade salt samples and 5 fine salt samples were collected from 7 major cities in Korea, from August to September 1987. These were analysed for content levels of Pb, Cd, Cu, Zn and Mn using Atomic Absorption Spectrophotometer. The results were as follows: 1. Lead contents in three type salts were N.D. - 1081.9 $\mu$g/kg and fourteen percent of the 114 samples exceeded the World Health Organization(WHO) criteria of 100$\mu$g/kg. Cadmium contents of samples were N.D.- 382C.5 $\mu$g/kg and five percent of the 114 samples were over the Spanish criteria of 500 $\mu$g/kg. Copper contents of samples were 8,9-214.9 $\mu$g/kg and there was not a sample over the World Health Organization(WHO) criteria of 500 $\mu$g/kg. Zinc contents ranged N.D. - 342.9 $\mu$g/kg and Manganese contents ranged N.D.- 8.31 mg/kg. 2. The comparison of heavy metal contents among the bay salts from three producing districts was significantly different in Pb, Cd and Cu contents. 3. The comparison of heavy metal contents between the bay salts and remade salts was not significantly different in Pb, Cd and Cu contents. 4. The contents of Pb, Cd, Zn and Mn in fine salts were much lower than those of bay salts and remade salts.

• Journal of the Korean Vacuum Society
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• v.2 no.1
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• pp.73-77
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• 1993

Hot-wall evaporation technique으로 세라믹 기판 위에 CdS 박막을 성장하였다. 이 때 증발원과 기판의 온도는 각각 570, 40$0^{\circ}C$이고 두께는 3$mu extrm{m}$이었다. 공기 중에서 열처리하여 감도(${\gamma}$), 광전류와 암전류의 비(pc/dc), 최대허용소비전력(MAPD), spectral response 및 응답시간 등을 측정하였다. $550^{\circ}C$, 30분간 열처리한 경우 가장 좋은 광전도 특성을 얻었으며 ${\gamma}$=0.89, pc/cd~104, MAPD: 492mW, rise time이 100ms, decay time이 260ms이었다.