• Title/Summary/Keyword: CD18

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Adhesion of CD40-stimulated Germinal Center B Cells to HK Cells Employs the CD11a/CD18-CD54 Interactions

  • Lee, Joonhee;Choe, Jongseon
    • IMMUNE NETWORK
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    • v.3 no.3
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    • pp.176-181
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    • 2003
  • Background: The molecular basis of follicular dendritic cells (FDC)-germinal center (GC) B cell interaction is largely unknown, although this cellular interaction is thought to be important for the whole process of GC B cell differentiation. Methods: Using FDC-like cells, HK, and highly purified GC B cells, we attempted to identify the molecules that play critical roles in the interactions between FDC and B cells. GC B cells were co-cultured with HK cells and soluble CD154 in the presence or absence of various function-blocking monoclonal antibodies to examine their effect on GC B cell binding to HK cells and B cell proliferation. Results: Anti-CD11a and anti-CD54 antibodies inhibited GC B cell binding to HK cells while anti-CD49d and anti-CD106 antibodies did not. GC B cell proliferation was not impaired by the disruption of GC B cell-HK cell adherence. Conclusion: Our results suggest that CD11a/CD18-CD54 interactions play an important roles in the initial binding of GC B cells to FDC and diffusible growth factors from FDC may be responsible the massive proliferation of GC B cells.

Characterization of CdS Thin Films and CdS/CdTe Heterojunction Prepared by Different Techniques (CdS 박막의 제조 방법에 따른 물성 및 CdS/CdTe 이종접합의 전기적 특성 분석)

  • Lee, Jae-Hyeong
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.18 no.3
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    • pp.199-205
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    • 2005
  • Polycrystalline cadmium sulfide(CdS) thin films were deposited on glass substrate by chemical bath deposition(CBD) and vacuum evaporation (VE) techniques. VE-CdS films consisted primarily of hexagonal phase, whereas CBD CdS films containing primarily the cubic form. VE-grown films were shown to have better crystallinity than CBD-grown films. The grain size of the CBD films is smaller than the ones of VE films. VE-CdS films exhibited relatively high transmittance in the above-gap region and band gap compared with CBD films. However, CdTe solar cells with these low quality CBD-CdS layers yield higher and more stable characteristics. Current-voltage-temperature measurements showed that the current transport for both cells was controlled by both tunneling and interface recombination but the cells with CBD-CdS displayed less tunneling.

The Expression of CD 18 on Ischemia- Reperfusion Injury of TRAM Flap of Rats (흰쥐의 복직근피부피판에 일으킨 허혈-재관류 손상에서 CD18의 발현)

  • Yoon, Sang Yup;Lee, Taik Jong;Hong, Joon Pio
    • Archives of Plastic Surgery
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    • v.33 no.6
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    • pp.737-741
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    • 2006
  • Purpose: This study was to evaluate the expression pattern of CD 18(leukocyte adhesion glycoprotein) in ischemia-reperfusion injury of TRAM flap of rats. Through this study, we can obtain more information about ischemia-reperfusion injury. We want to develop specific medicine to improve the survival rate of TRAM flap in the future. Methods: A TRAM flap supplied by a single pedicle superior epigastric artery and vein was elevated on 60 Sprauge-Dawley rats. The rats were divide into 6 groups (each group n=10); Group O: sham, no ischemia-reperfusion injury, Group I: 2 hour reperfusion after 4 hour ischemia, Group II: 4 hour reperfusion after 4 hour ischemia, Group III: 8 hour reperfusion after 4 hour ischemia, Group IV: 12 hour reperfusion after 4 hour ischemia, and Group V: 24 hour reperfusion after 4 hour ischemia. This study consisted of gross examination for flap survival and flow cytometry study of CD18 on neutrophils. Results: The gross measurement of the flap showed different survival rate in group I(71%), II(68%), III(37%), IV(34%) and V(34%). All experimental groups showed an increase in the expression of CD18 compared to group O. The expression of CD18 was rapidly increased in ascending order in group I, II and III. But, the expression of CD18 was maintained in group IV and V. Conclusion: The results can be implemented in the study to develop drugs which are capable of reducing ischemia-reperfusion injury in microsurgical breast reconstruction.

Roles of Neutral Sphingomyelinase 1 on CD95-Mediated Apoptosis in Human Jurkat T Lymphocytes

  • Lee, Hyun-Min;Surh, Bo-Young;Chun, Young-Jin
    • Biomolecules & Therapeutics
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    • v.18 no.3
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    • pp.262-270
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    • 2010
  • CD95 receptor is a member of tumor necrosis factor receptor family that mediates apoptosis in many cell types when bound by CD95 ligand or cross-linked by agonistic anti-CD95 antibodies. To determine the role of neutral sphingomyelinase (nSMase) on CD95-mediatd apoptosis, human Jurkat T lymphocytes were exposed to recombinant human CD95 ligand. Treatment with CD95 ligand induced cell death in a concentration and time-dependent manner. CD95-induced cell death was suppressed by inhibitors of SMase such as AY9944 or desipramine. Transfection with human nSMase1 siRNA plasmid into CD95 ligand-treated cells significantly prevented CD95-mediated cell death. CD95-mediated elevation of intracellular ceramide level detected by FACS analysis with anti-ceramide antibody was also decreased by nSMase1 siRNA. Knock-down of nSMase1 expression also blocked cytochrome c release into cytosol and caspase-3 cleavage in CD95-treated cells. Taken together, these results suggest that nSMase1 may play an important role in CD95-mediated apoptotic cell death in Jurkat T cells.

Cadmium contents in tissues of slaughtered deer in Korea (도축 사슴의 체조직내 카드뮴 함량에 관한 조사 연구)

  • 장종식;권오덕
    • Korean Journal of Veterinary Service
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    • v.24 no.1
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    • pp.95-99
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    • 2001
  • In order to clarify the cadmium(Cd) contents of normal deer, tissue samples including kidney, liver, muscle, spleen, heart, lung, rumen, abomasum, intestine, skin and bone were collected from 18 deer which were slaughtered in Korea. All samples were burned to ashes and analyzed for Cd contents by atomic absorption spectrophotometric method. The mean Cd contents in \mu\textrm{g}$/g wet matter for 18 deer were liver 0.013, kidney 0.286, muscle 0.010, spleen 0.001, heart 0.007, lung 0.005, men 0.019, abomasum 0.016, intestine 0.018, skin 0.016 and bone 0.312, respectively. Two samples in kidney, one sample in muscle out of 18 deer showed higher Cd contents than normal limit value of 0.50\mu\textrm{g}$/g for liver and kidney, 0.10\mu\textrm{g}$/g for muscle. In addition, three deer also showed higher Cd contents in one body tissue than normal limit value which reported foreign countries.

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The effect of Indole acetic acid on the accumulation of Cd2+ and growth of Cd2+ - treated Commelina communis L. (닭의장풀에 Cd2+ 처리시 Cd2+ 흡수와 생장에 미치는 indole acetic acid의 영향)

  • Lee, Jun Sang
    • Journal of Environmental Science International
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    • v.13 no.6
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    • pp.513-518
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    • 2004
  • 3-weeks old Commelina was transferred to and grown in Hoagland solution (Control, $100\muM$ $Cd^{2+}$, $100\muM$ $Cd^{2+}$+ $100\muM$ IAA, $100\muM$ $Cd^{2+}$+ $100\muM$ IAA + 2 mM sucrose) for 3 weeks and then the effects of indole acetic acid (IAA) on the accumulation of $Cd^{2+}$ and growth of $Cd^{2+}$-treated Commelina were investigated. In the treatment of $Cd^{2+}$, $Cd^{2+}$ was uptaked to 1.74, and 51.36 ${\mu}g/g$ frwt. at the first week, but for three weeks, 0.51 and 34,53 ${\mu}g/g$ frwt, in leaf and stem respectively. When IAA was treated along with $Cd^{2+}$, $Cd^{2+}$ was uptaked to 0.18 and 8.63 ${\mu}g/g$ fiwt, at the first week, and for the incubation of 3 weeks, 0,51 and 45.0 ${\mu}g/g$ fiwt. in leaf and stem. In case of $Cd^{2+}$+IAA+sucrose, $Cd^{2+}$ was uptaked to 1.45 and 18.33 ${\mu}g/g$ frwt. at the first week, but for 3 weeks, 0,51 and 25.45 ${\mu}g/g$ fiwt. in leaf and stem. Likewise $Cd^{2+}$ uptake, the growth was also affected by $Cd^{2+}$ and IAA. During the incubation of 3 weeks, $Cd^{2+}$ reduced the stem growth about 8% in all weeks, but the treatment of IAA recovered the inhibition of stem growth caused by $Cd^{2+}$ to the degree of the control Therefore, it could be concluded that IAA altered the pattern of $Cd^{2+}$ uptake and the growth which were supposed to change $Cd^{2+}$ toxicity.

CD-ROM과 멀티미디어

  • Korea Database Promotion Center
    • Digital Contents
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    • no.12 s.7
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    • pp.18-18
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    • 1993
  • 93년 11월 멀티미디어 전문 리포트인 멀티미디어 모니터에 충격적인 기사가 실렸다. 영어를 그대로 사용하면 "In the CD-ROM market, all that glitters is not consumer multimedia"이다. 내용을 일부 요약하면 다음과 같다.

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Changes of Contrast Sensitivity with Decreasing Luminance in Photopic Conditions (명소시 상태에서 휘도 감소에 따른 대비감도 변화)

  • Lee, Eun Jin;Yoon, Moon Joo;Kim, Soo-Hyun;Yang, Gye Tak;Jeong, Ju-Hyun;Kim, Hyun Jung;Kim, Hyun Il;Choi, Eun Jung
    • Journal of Korean Ophthalmic Optics Society
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    • v.17 no.4
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    • pp.411-417
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    • 2012
  • Purpose: To investigate changes of contrast sensitivity with decreasing luminance in photopic conditions. Methods: Contrast sensitivities for 63 university students were investigated at 5 different luminance levels using FACT chart. Results: All contrast sensitivities measured were in the normal range. The peak of contrast sensitivity function was observed to move from 6 to 3 cpd between 40 and $10cd/m^2$. From an intersection point of two hypothetical lines related with contrast sensitivity for 6 and 3 cpd, we could evaluated that the peak moved from 6 to 3 cpd at about $25cd/m^2$. When luminance decreases from 120 to $10cd/m^2$, contrast sensitivity loss at 6 cpd was most, while contrast sensitivity at 1.5 cpd changed little. We could evaluate that at luminance of more than $25cd/m^2$ evaluated approximately, contrast sensitivity was high in the following order of spatial frequency: 18< 12<1.5<3<6 cpd, while at luminance of less than about $25cd/m^2$ as follows: 18<12<1.5<6<3 cpd. Contrast sensitivity losses with decreasing luminance were also evaluated using the decrements and decrement rates of contrast sensitivity. The decrement of contrast sensitivity was the greatest at 6 cpd, while the decrement rate was the greatest at 8 cpd. Conclusions: We propose that evaluation methods used in this research may be available as another methods to evaluate the changes of contrast sensitivity.