• Title/Summary/Keyword: CCR1

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Carbon Catabolite Repression (CCR) of Expression of the XylanaseA Gene of Bacillus stearothermophilus No.236

  • Ha, Gyong-Sik;Choi, Il-Dong;Choi, Yong-Jin
    • Journal of Microbiology and Biotechnology
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    • v.11 no.1
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    • pp.131-137
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    • 2001
  • Previous work has identified that only the catabolite responsive element A (creA; previously called cre-2) out of two potential cre sequences (cre-1: nucleotide +160 to +173 and cre-2: +173 to +186), recognized within the coding region of the xylanaseA gene (xynA) of Bacillus stearothermophilus No.236, was actually, was actually involved in the carbon catabolite repression(CCR) of xynA expression in B. subtilis. However, the level of CCR of xynA expression in the original B.stearothermophilus No.236 strain (70-fold repression). Therefore, to search for an additional cre element in the promoter region, the upstream region of the xynA gene was subcloned by chromosome walking, and as a result, another potential cre element (nucleotide -124∼-137; designated creB) was recognized in this region. The cre-like sequence revealed a high homology to the cre consensus sequence. The xylanase activity of B. subtilis MW15 bearing pWPBR14 (containing creA and creB) cultured in a medium containing xylose as the sole carbon source was about 7.7 times higher than that observed for the same culture containing glucose. B. subtilis MW15 bearing pWPBR23 (containing only creA) produced an activity about 2.4 times higher. This pattern of CCR was confirmed using derivatives of xynA::aprA fusion plasmids. Furthermore, a measurement of the amounts of the xynA transcript showed a similar pattern as that for the production of xylanase. In addition, the synthesis of xylanase in B. subtilis QB7115 [a catabolite control protein A (ccpA) mutant strain] carrying pWPBR14 was almost completely relieved from glucose repression. Together, these results lead to a conclusion that the CCR of the expression of the xynA gene is mediated by CcpA binding at creA and creB sites in B. subtilis.

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Effectiveness of endodontic retreatment using WaveOne Primary files in reciprocating and rotary motions

  • Patricia Marton Costa;Renata Maira de Souza Leal;Guilherme Hiroshi Yamanari;Bruno Cavalini Cavenago;Marco Antonio Hungaro Duarte
    • Restorative Dentistry and Endodontics
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    • v.48 no.2
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    • pp.15.1-15.7
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    • 2023
  • Objectives: This study evaluated the efficiency of WaveOne Primary files (Dentsply Sirona) for removing root canal fillings with 2 types of movement: reciprocating (RCP) and continuous counterclockwise rotation (CCR). Materials and Methods: Twenty mandibular incisors were prepared with a RCP instrument (25.08) and filled using the Tagger hybrid obturation technique. The teeth were retreated with a WaveOne Primary file and randomly allocated to 2 experimental retreatment groups (n = 10) according to movement type: RCP and CCR. The root canals were emptied of filling material in the first 3 steps of insertion, until reaching the working length. The timing of retreatment and procedure errors were recorded for all samples. The specimens were scanned before and after the retreatment procedure with micro-computed tomography to calculate the percentage and volume (mm3) of the residual filling material. The results were statistically evaluated using paired and independent t-tests, with a significance level set at 5%. Results: No significant difference was found in the timing of filling removal between the groups, with a mean of 322 seconds (RCP) and 327 seconds (CCR) (p < 0.05). There were 6 instrument fractures: 1 in a RCP motion file and 5 in continuous rotation files. The volumes of residual filling material were similar (9.94% for RCP and 15.94% for CCR; p > 0.05). Conclusions: The WaveOne Primary files used in retreatment performed similarly in both RCP and CCR movements. Neither movement type completely removed the obturation material, but the RCP movement provided greater safety.

Relative Efficiency and Productivity Change of the Korean Non-Life Insurance Firms (국내 손해보험회사의 상대적 효율성 및 생산성 변화)

  • Kang, Ho-Jung
    • The Journal of the Korea Contents Association
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    • v.9 no.5
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    • pp.224-229
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    • 2009
  • This study measures the relative efficiency and productivity change of the Korean Non-Life Insurance Firms using DEA model and Malmquist Index for 2004-2007. The main results of this study can by summarized as follows. First, in case of efficiency of CCR for 2004-2007, the number of efficient firms(CCR value is one) are one firm, one firm, one firm, one firm respectively. Second, in case of efficiency of BCC for 2004-2007, the number of efficient firms(BCC value is one) are five firms, five firms, six firms, six firms respectively. Third, In case of return to scale for 2004-2007, DRS are five firms, DRS are five firms, DRS are six firms, DRS are six firms respectively, Fourth, Malmquist Index representing productivity change for 2004-2007 are 0.99 in 2004-2005, 1.04 in 2005-2006, 1.06 in 2006-2007.

Analysis of Three-Phase Multiple Access with Continual Contention Resolution (TPMA-CCR) for Wireless Multi-Hop Ad Hoc Networks

  • Choi, Yeong-Yoon;Nosratinia, Aria
    • Journal of Communications and Networks
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    • v.13 no.1
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    • pp.43-49
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    • 2011
  • In this paper, a new medium access control (MAC) protocol entitled three-phase multiple access with continual contention resolution (TPMA-CCR) is proposed for wireless multi-hop ad hoc networks. This work is motivated by the previously known three-phase multiple access (TPMA) scheme of Hou and Tsai [2] which is the suitable MAC protocol for clustering multi-hop ad hoc networks owing to its beneficial attributes such as easy collision detectible, anonymous acknowledgment (ACK), and simple signaling format for the broadcast-natured networks. The new TPMA-CCR is designed to let all contending nodes participate in contentions for a medium access more aggressively than the original TPMA and with continual resolving procedures as well. Through the systematical performance analysis of the suggested protocol, it is also shown that the maximum throughput of the new protocol is not only superior to the original TPMA, but also improves on the conventional slotted carrier sense multiple access (CSMA) under certain circumstances. Thus, in terms of performance, TPMA-CCR can provide an attractive alternative to other contention-based MAC protocols for multi-hop ad hoc networks.

CCR Expression of Bronchoalveolar Lavage Fluid (BALF) Neutrophils and Chemotactic Activity of BALF

  • Choi, Eu-Gene;Yang, Eun-Ju;Kim, Dong-Hee;Lee, Ji-Sook;Kim, In-Sik
    • Biomedical Science Letters
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    • v.17 no.1
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    • pp.89-93
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    • 2011
  • Asthma is an inflammatory airway disease and is characterized by the releases of inflammatory mediators including chemokines. They are mainly associated with the recruitment, activation and dysregulation of specific inflammatory cells, especially neutrophils in neutrophilic asthma. CC chemokines bind to CC chemokine receptors (CCRs) in the surface of their target cells. The aims of this study are to examine the CCR expression in neutrophils of bronchoalveolar lavage fluid (BALF) of asthmatic patients and to determine the alternation of migration and apoptosis of neutrophils by the BALF. We demonstrate that CCR3 strongly expresses in BALF neutrophils of asthmatic patients as compared to other CCRs and increases during apoptosis of the BALF neutrophils. The migration of asthmatic blood neutrophils increases in response to asthmatic BALF as compared to BALF of normal volunteer. In addition, asthmatic BALF includes the higher levels of IL-8 protein than normal BALF and it has no effect on apoptosis of asthmatic blood neutrophils. Taken together, our results indicate that CCR3 expression may be associated with unknown function of asthmatic BALF neutrophils and BALF may be involved in the recruitment of neutrophils into the airway, but not in the neutrophils apoptosis.

Management Efficiency of Korean Information and Communication Enterprise (국내 정보통신업의 경영효율성)

  • Kim, Jong-Ki;Kang, Da-Yeon
    • Journal of Korea Society of Industrial Information Systems
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    • v.14 no.1
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    • pp.32-42
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    • 2009
  • Information and communication industry, which has been growing rapidly, is now leading the global economic growth, contributing a ripple effect on general business and economic structure. It is said that information and communication industry enables swift economic growth taking a role as a basic industry of information-oriented society. Especially, rapid technical innovation promotes mutual growth of other related industries and it is technology-intensive. The purpose of this research is to analyze the management efficiency of information and communication industry using DEA method, and finally suggests numerical value for inefficient organizations to improve their efficiency. CCR-I, BCC-I efficiency and RTS(Returns to scale) of 29 companies engaged in information and communication industry was evaluated. With the results, we suggested the model companies for benchmarking in information and communication industry. To summarize, 7 companies were selected with CCR-I efficiency rate 1, and 12 companies with BCC-I efficiency rate 1. RTS was finalized with 8 IRS companies, 10 DRS companies, and 11 CRS companies.

Efficiency Analysis of Construction Firms Using a Combined AHP and DEA Model (AHP와 DEA 결합모델을 이용한 상장 건설기업의 효율성 분석)

  • Seo, Kwang-Kyu;Choi, Da-Young
    • The Journal of the Korea Contents Association
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    • v.11 no.6
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    • pp.302-310
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    • 2011
  • Recently, many construction firms fall on hard times because construction business continues to stagnate. In this paper, we analyze the efficiency of the listed construction firms using a combined AHP and DEA model. In order to determine the input and output factors of DEA, the AHP model is applied to evaluate the importance of input and output factors. The benchmarking companies and efficiency value for the construction firms with inefficiency are also provided to improve the their efficiency. We analyzed the 57 listed companies consisted of 36 listed on KOSPI and 21 listed on KOSDAQ at the end of 2009. The analysis results show that eleven companies whose values of CCR are 1, and fourteen enterprises whose values of BCC efficiency are 1. In additions, the eleven firms have the scalability efficiency. Finally, we test the correlation between efficiency and the stock price and the correlation coefficient of efficiency group is 0.7 in the CCR model.

A Study on Joining Method of BSCCO(223) Multifilamentary Tape (BSCCO(2223) 다심 초전도 선재의 접합공정 연구)

  • 김정호;김규태;주진호;나완수
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.14 no.6
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    • pp.511-517
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    • 2001
  • We evaluated the electrical properties of 37 multifilamentary jointed tapes processed by superconducting joint. In the superconducting joining method, a lap-joint was used. Tapes were selectively etched, and exposed superconducting cores of the two tapes were brought into contact with each other and then only the joined region was uniaxially pressed in the range of 1,000 to 2,50 MPa. The critical current ratio(CCR) and n-value of the jointed tape were evaluated as a function of uniaxial pressure and number of step in the contacting region. It was observed that the CCR was dependent on the number of step, but almost independent of uniaxial pressure. The highest critical current ratio and n-value were obtained to be 58% and 26%, respectively, for the jointed tape to the tape itself.

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A Study on the Relative Operational Efficiency of Foreign Port Transportation Industry (외항운송업의 상대적 운영효율성 분석)

  • Kang, Da-Yeon;Lee, Ki-Se
    • The Journal of the Korea Contents Association
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    • v.21 no.4
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    • pp.88-95
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    • 2021
  • In Korea, which achieved economic growth due to export industries, the economic importance of the foreign port transportation industry is very great. The government took the initiative in fostering the foreign port transportation industry, and as a result, it developed into one of the top 10 foreign port transport countries in the world. However, due to the poor management of the small and medium-sized foreign port transport business and the trade dispute between countries, the foreign port transportation industry has faced a long-term invasion. Therefore, in order for these companies to recover again, they will, among other things, have to reorganize their operational inefficiency to carry out efficient production activities. In this study, we want to analyze the management efficiency of the top 10% companies in the unlisted domestic outbound shipping industry, present the project value of inefficient enterprises, and confirm the reference collection of companies that may be subject to benchmarking. For this purpose, a total of 10 foreign port transportation industry were analysed for efficiency and scale profitability (RTS) of the CCR and BCC models. The analysis showed that there were a total of six entities with BCC efficiency and five entities with CCR efficiency. In addition, there were a total of five entities with both BCC and CCR efficiency.