• Journal of Veterinary Science
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• 제21권1호
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• pp.2.1-2.14
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• 2020

The emergence of livestock-associated (LA)-methicillin-resistant Staphylococcus aureus (MRSA) in livestock animal has become a significant zoonotic concern. In the present study, we investigated nationwide prevalence of LA-MRSA across pork production chain including pig farms, slaughterhouses, and retail markets. A total of 40 MRSA strains were isolated during the investigation and the overall prevalence of MRSA was 3.4% (n = 37), 0.6% (n = 2), and 0.4% (n = 1) in pig farms, slaughterhouses, and retail markets, respectively. Multilocus sequence typing analyses revealed that the 2 most significant clonal lineages in pork production chain in Korea were ST398 (n = 25) and ST541 (n = 6). All of the 40 MRSA isolates were further characterized to investigate key genotypic and phenotypic correlates associated with the emergence and spread of clonal complex 398 (CC398; ST398, and ST541) LA-MRSA. Although the prevalence of swine-associated MRSA was still relatively low and mostly restricted to pig farms, multidrug-resistant CC398 LA-MRSA isolates with new spa types (t18102 and t18103) were identified as a major clonal lineage. The CC398 LA-MRSA strains tended to exhibit increased levels of multiple drug resistance (MDR) phenotype compared with non-CC398 MRSA strains. Of note, in comparison with non-CC398 MRSA isolates, CC398 LA-MRSA isolates exhibited significantly enhanced tetracycline (TET) and zinc resistance. These findings suggested that co-selection pressure associated with MDR phenotype, especially TET resistance, and zinc resistance may have played a significant role in the emergence and persistence of CC398 LA-MRSA in pig farms in Korea.

• 한국의학물리학회지:의학물리
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• 제22권3호
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• pp.148-154
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• 2011

TRS-398 프로토콜에서 중심전극에 대한 최근의 연구 결과들을 적용하여 선질인자($k_{Q,Q_0}$)를 평가하였다. 대상 전리함은 PTW-31010과 IBA-CC13이었다. 광자선 및 전자선 선질의 함수로서 선질인자를 계산하였으며 현재 TRS-398 프로토콜의 값들과 비교하였다. PTW-31010 전리함과 같이 알루미늄 전극으로 구성된 전리함에 대하여 새로운 보정을 취하는 경우에 선질인자가 광자선에서 최대 0.4% 그리고 전자선에서 최대 0.9% 증가하는 것으로 나타났다. 또한 IBA-CC13과 같이 C-552 전극을 사용하는 전리함의 경우에 기존의 보정체계를 그대로 적용할 수 있음을 확인하였다.

• 한국미생물·생명공학회지
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• 제51권1호
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• pp.132-133
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• 2023

Clonal complex (CC) 398 community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) has emerged worldwide in a variety of livestock animals and humans. We report complete genome sequences of Panton-Valentine leucocidin (PVL) and immune evasion cluster (IEC) gene-positive CC398 MRSA strains isolated from patients in Korea.

• Journal of Chest Surgery
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• 제19권3호
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• pp.391-398
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• 1986

From Jan. 1983 to Dec. 1985, seven cases of pectus excavatum, six were male and one female, were underwent an operation at the Department of Thoracic and Cardiovascular Surgery, Kyungpook National University Hospital. The ages of patients ranged from 6 to 27 years. They all had symptoms of feeling inferiority about chest deformity. The concavity on the funnel chest varied in its extent, and the severity, which was measured by water volume filled into it, varied from 59.5cc/m2 to 129.9cc/m2. All but one patients were approached through a bilateral transverse submammary incision and one approached through a vertical midline incision. Successful surgical correction required resection of all deformed costal cartilages with transverse anterior osteotomy and internal fixation using retrosternal metal bar. No serious complication have followed the use of this technique, but minor complications such as serous accumulation, pneumothorax and strut migration have been experienced. All patients were satisfactory about the surgical results.

• Biomolecules & Therapeutics
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• 제21권5호
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• pp.398-404
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• 2013

During a screening program to search the anticolitic herbal medicines, 80% ethanol extract of the rhizome of Anemarrhena asphodeloides (AA) was found to potently inhibit the expression of proinflammatory cytokines TNF-${\alpha}$ and IL-1${\beta}$, as well as the activation of NF-${\kappa}B$ in LPS-stimulated colonic macrophages, followed by that of the rhizome of C. chinensis (CC). AA also potently inhibited TNBS-induced colitic markers, shortening of the colon and increase of macroscopic score, myeloperoxidase activity, TNF-${\alpha}$, IL-1${\beta}$, and IL-6, in mice. The synergistic effect of CC against the anticolitic effect of AA was investigated. CC synergistically inhibited the anticolitic effect of AA. AC-mix (AA+CC, 1:1) potently inhibited them. AC-mix also inhibited the activation of NF-${\kappa}B$, as well as the expression of TNF-${\alpha}$, IL-1${\beta}$, IL-6, iNOS and COX-2. The effects of AC-mix against oxazolone-induced colitis were investigated in mice. AC-mix also potently inhibited oxazolone-induced inflammatory markers, colon shortening, macroscopic score, myeloperoxidase activity, NF-${\kappa}B$ activation and proinflammatory cytokines. Overall, the anti-colitic effect of AC-mix was superior to that of mesalazine. Based on these findings, AC-mix may improve colitis by inhibiting NF-${\kappa}B$ activation.

• Journal of Veterinary Science
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• 제21권4호
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• pp.54.1-54.11
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• 2020

Background: Methicillin-resistant Staphylococcus aureus (MRSA) is a leading cause of severe infections in humans and animals worldwide. Studies elucidating the population structure, staphylococcal cassette chromosome mec types, resistance phenotypes, and virulence gene profiles of animal-associated MRSA are needed to understand spread and transmission. Objectives: The objective of this study was to determine 1) clonal complexes and spa types, 2) resistance phenotypes, and 3) virulence/resistance gene profiles of MRSA isolated from animals in Switzerland. Methods: We analyzed 31 presumptive MRSA isolates collected from clinical infections in horses, dogs, cattle, sheep, and pigs, which had tested positive in the Staphaurex Latex Agglutination Test. The isolates were characterized by spa typing and DNA microarray profiling. In addition, we performed antimicrobial susceptibility testing using the VITEK 2 Compact system. Results: Characterization of the 31 presumptive MRSA isolates revealed 3 methicillinresistant Staphylococcus pseudintermedius isolates, which were able to grow on MRSA2 Brilliance agar. Of the 28 MRSA isolates, the majority was assigned to CC398 (86%), but CC8 (11%) and CC1 (4%) were also detected. The predominant spa type was t011 (n = 23), followed by t009 (n = 2), t034 (n = 1), t008 (n = 1), and t127 (n = 1). Conclusions: The results of this study extend the current body of knowledge on the population structure, resistance phenotypes, and virulence and resistance gene profiles of MRSA from livestock and companion animals.

• 대한기계학회논문집
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• 제10권3호
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• pp.392-398
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• 1986

본 연구에서는 실험적 방법을 통하여 엔진 배기관의 굴곡 형상이 배기 소음에 미치는 영향에 대해서 고찰하였다. 엔진으로는 4기통, 배기량 2164cc의 디이젤 엔진 을 사용하였으며 엔진의 보통 사용 범위인 1200∼3200rpm, 드로틀밸브의 개방도 25∼를 100% 구간에서 실험을 수행하였다. 굴곡부의 형상으로는 대부분의 배기관 굴곡이 원 호 형태임을 고헌하여 원호형의 굴곡관과 관의 꺽임부의 영향을 고찰하기 위한 직각형 굴곡관의 두가지 형태를 택하였따. 각 엔진의 운전 조건에서 굴곡관의 형상 치수를 바꾸어 가며 배기 소음의 음압(sound pressure level, SPL)과 스펙트럼을 얻었으며 이 들을 상호 비교 검토하여 배기관 굴곡 형상의 주요 설계 변수에 의한 배기 소음의 영 향을 고찰하였다.

• 한국항행학회논문지
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• 제13권3호
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• pp.393-398
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• 2009

본 논문에서는 Zigbee를 이용한 자동인식 시스템에 관한 연구를 하였다. RFID 시스템은 통신 거리가 제한적이고, 통신망이 손실되면 통신이 불가능하다. 따라서 RFID 시스템과 모니터링 시스템과의 통신망 설치가 어렵거나, RFID 시스템의 설치 위치가 유동적인 지역에서는 설치가 쉽고 낮은 비용의 무선 시스템이 필요하다. 제안된 자동인식 시스템은 멜렉시스사의 MLX12115 RFID 칩을 이용한 13.56MHz RFID 시스템과, 칩콘사의 CC2420 지그비 칩을 이용한 무선 센서노드 시스템을 하나의 시스템으로 구현하였다. 연구결과, 무선으로 Tag의 정보 취득이 용이하며, 회로의 구성이 간단하고 작은 크기의 자동 인식시스템을 구현하였다.

• Clinical and Experimental Reproductive Medicine
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• 제32권3호
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• pp.279-286
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• 2005

Objective: Although several genetic factors have been associated with defects in human spermatogenesis, the unambiguous causative genes have not been elucidated. The male infertility by haploinsufficiency of PRM1 or PRM2 has been reported in mouse model. The aim of this study was to identify the single nucleotide polymorphisms (SNPs) of PRM1 and PRM2, related to the genotype of Korean infertile men. Methods: Genomic DNAs were extracted from peripheral bloods of infertile men with oligozoospermia or azoospermia, and analyzed using polymerase chain reaction (PCR) and direct sequencing. We carried out the direct sequencing analysis of amplified fragments in PRM1 (557 nucleotides from -42 to 515) and PRM2 (599 nucleotides from 49 to 648) genes, respectively. Results: Three SNPs of coding region in the PRM1 gene was found in the analysis of 130 infertile men. However, the SNPs at a133g (aa 96.9%, ag 3.1% and gg 0.0%), c160a (cc 99.2%, ca 0.8% and aa 0.0%) and c321a (cc 56.9%, ca 35.4% and aa 7.7%) coded the same amino acids, in terms of silence phenotypes. On the other hand, as results of the PRM2 gene sequencing in 164 infertile men, only two SNPs, g398c (gg 62.2%, gc 31.1% and ga 6.7%) and a473c (aa 63.4%, ac 29.9% and cc 6.7%), were identified in the intron of the PRM2 gene. Conclusions: There was no mutation and significant SNPs on PRM1 and PRM2 gene in Korean infertile men. These results suggest that the PRM1 and PRM2 genes are highly conserved and essential for normal fertility of men.

• 전기학회논문지
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• 제57권3호
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• pp.398-402
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• 2008

Recently, the development of superconducting fault current limiters (SFCLs) has been required as power demands increase in the power system. A distribution-level prototype resistive SFCL using coated conductor (CC) has been developed by Hyundai Heavy Industries Co., Ltd. and Yonsei University for the first time in the world. The ratings of the SFCL are 13.2kV/630A at normal operating condition. A novel non-inductive winding method is used in fabricating coils so there is almost zero impedance during normal operation. The distribution SFCL is cooled by sub-cooled liquid nitrogen $(LN_2)$ of 65K and 3 bar to enhance cryo-dielectric performance, critical current density, and thermal conductivity. In order to make reliable operation of an SFCL in real power systems, we monitored and controled its operation conditions by using supervisory control and data acquisition (SCADA) method. Thus, a monitoring system for the SFCL employing information technology (IT) is proposed and developed to be on the lookout for the operation conditions such as inside temperature, inside pressure, $LN_2$ level, voltage and current. Since operation temperature should be kept constant, bang-bang control for temperature feedback with a heater attached to the cold head of cryo-cooler is applied to the system. Short-circuit tests with prospective fault current of 10kA and AC dielectric withstand voltage tests up to 143kV for 1 minute were successfully performed at Korea Electrotechnology Research Institute. This paper deals with the development of a distribution level SFCL and its monitoring system for reliable operation.