• Journal of Microbiology and Biotechnology
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• v.30 no.1
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• pp.21-30
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• 2020

Natural products are widely used due to their various biological activities which include anti-inflammatory, antioxidant, and anti-obesity effects. In this study, we determined the antioxidative and anti-obesity effects of Polygonum cuspidatum 50% ethanol extract (PEE). The antioxidative effect of PEE was evaluated using its radical scavenging activity, total phenolic content, and reducing power. The anti-obesity effect of PEE was investigated using 3T3-L1 adipocytes. The antioxidative activity of PEE was progressively increased in various concentrations, mainly due to the presence of phenolic compounds. PEE also alleviated lipid accumulation on 3T3-L1 adipocytes and downregulated the mRNA and protein production of adipogenesis-related (SREBP-1c, PPARγ, C/EBPα) and lipogenesis-related (aP2, FAS, ACC) markers. Furthermore, we found that the inhibitory effect on lipid accumulation via PEE was caused by the alleviation of NF-κB, p38 MAPK, ERK1/2, and JNK at the protein level. Taken together, our results imply that PEE is a potential antioxidant that can prevent obesity-associated disorders.

• Journal of Acupuncture Research
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• v.21 no.1
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• pp.33-50
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• 2004

Objective: The aim of this study was to investigate anti-ischemic effect of LR1 HT8 Reduction in Acupuncture Methods: I designed to investigate whether LR1 HT8 Reduction in Acupuncture affects cerebral hemodynamics [regional cerebral blood flow(rCBF), pial arterial diameter(PAD), mean arterial blood pressure(MABP)] in normal rats and to make manifest whether LR1 HT8 Reduction in Acupuncture is mediated by cyclooxygenase or guanylate cyclase. The changes of rCBF and MABP were determinated by laser-doppler flowmetry(LDF), and the change of PAD was de terminated by video microscope and width analyzer. Results: The results were as follows; 1. LR1 Reduction in Acupuncture was increased rCBF and PAD, but decreased MABP. 2. HT8 Reduction in Acupuncture was significantly increased rCBF, but decreased MABP, and increased PAD. 3. LR1 HT8 Reduction in Acupuncture was significantly increased rCBF, PAD, but decreased MABP after withdrawing of the needle. This results suggest that LR1 HT8 Reduction in Acupuncture increased significantly rCBF by dilating PAD. 4. Pretreatment with indomethacin(1mg/kg, i,v.) was significantly inhibited LR1 HT8 Reduction in Acupuncture induced increase of rCBF and PAD, but increased LR1 HT8 Reduction in Acupuncture induced decrease of MABP after withdrawing of the needle. 5. Pretreatment with methylene blue($10{\mu}g/kg$, i,v.) was decreased LR1 HT8 Reduction in Acupuncture induced increase of rCBF and PAD, but accelerated LR1 HT8 Reduction in Acupuncture induced decrease of MABP. Conclusions: I suggest that LR1 HT8 Reduction in Acupuncture has an anti-ischemic effect through the improvement of cerebral hemodynamics, and the mechanism is mediated by cyclooxygenase.

• Journal of Acupuncture Research
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• v.25 no.6
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• pp.1-12
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• 2008

Objectives : Joongjeo($TE_3$) Supplementation Aekmoon($TE_2$) Draining is a method belongs to Ohaeng-acupuncture, using directional supplementation and draining. Methods : This study was designed to investigate the effects of $TE_3$ supplementation $TE_2$ draining on changes in cerebral blood flow(rCBF) and mean arterial blood pressure(MABP) in normal rats. For these reasons, the present author investigated rCBF and MABP using laser doppler flowmeter in normal rats. In addtion, the present author also investigated action mechanisms of $TE_3$ supplementation $TE_3$ draining on changes in rCBF and MABP too. Results : In this results, $TE_3$ supplementation $TE_2$ draining elevated rCBF in time-dependent manner, but MABP levels decresed by $TE_3$ supplementation $TE_2$ draining. Pre-treatment with indomethacin (IDM), an inhibitor of cyclooxygenase, inhibited increase of rCBF effectively. But pre-treatment with methylene blue(MTB), an inhibitor of guanylate cyclase, decreased rCBF levels. In addition, pre-treatment with IDM also decreased MABP levels, but pre-treatement with MTB increased MABP levels. Conclusions : In conclusion, these results suggest that $TE_3$ supplementation $TE_2$ draining is effective to treat patient with disease related to cerebral ischemia, because $TE_3$ supplementation $TE_2$ draining can increase rCBF. In addition, the mechanisms are thought to be related to guanylate cyclase pathways.

• The Journal of Internal Korean Medicine
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• v.23 no.1
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• pp.91-98
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• 2002

Objective : Bangpungtongseongsan(BTS) has been used in oriental medicine for many centuries as a prescription for a heat syndrome of apoplexy. The effects of BTS on the vascular system are not well known. This study is designed to identify the effects of BTS on the regional cerebral blood flow(rCBF), arterial blood pressure and action mechanism in mice. Methods : We measured the change of rCBF and BP by BTS. Secondly, we investigated changes of rCBF and BP for 30 minutes interval after venous inject 0.01, 0.1, 1.0, 10.0mg/kg each density BTS infusion into mice, which were premanaged with methylene blue(10mg/kg, i.v), indomethacin(3mg/kg, i.v.), propranolo(3mg/kg, i.v.) for 30minutes. Results : rCBF increased significantly by BTS in a dose-dependent and BP was not affected by BTS in mice. Pretreatment with methylene blue significantly inhibited rCBF increased by BTS and aceelrated BP not affected by BTS. Pretreatment with indomethacin significantly inhibited rCBF increased by BTS and inhibited BP not affected by BTS. Conclusion : These results show that BTS causes the increasement of rCBF and non-change of BP. The action mechanism is related to prostaglandid activated by cyclooxygenase.

• Journal of Physiology & Pathology in Korean Medicine
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• v.18 no.1
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• pp.75-83
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• 2004

This experimental study was designed to investigate the effects of Sagunja-tang(SGJT), Ijin-tang(IJT), Yukgunja-tang(YGJT) on the change of cerebral hemodynamics [regional cerebral blood f1ow(rCBF), mean arterial blood pressure(MABP), and pial arterial diameter (PAD)] in normal rats, and further to determine the mechanism of action of YGJT. And, this Study was designed to investigate whether YGJT inhibit lactate dehydrogenase(LDH) activity in neuronal cells. The results were as follows ; 1. SGJT significantly increased rCBF but MABP was not changed comparing with normal MABP(l00 %). This results were suggested that SGJT significantly increased rCBF by dilating PAD. 2. IJT significantly decreased rCBF in a dose-dependent, but significantly increased MABP in a dose-dependent. This results were suggested that IJT significantly decreased rCBF by contracting PAD. 3. YGJT significantly increased rCBF and PAD in a dose-dependent, and YGJT increased MABP compared with normal MABP(100 %). This results were suggested that YGJT significantly increased rCBF by dilating PAD. 4. The YGJT-induced increase in rCBF was significantly accelerated by pretreatment with indomethacin (IDN, 1 mg/kg, i.p.), an inhibitor of cyclooxygenase but was significantly inhibited by methylene blue (MTB, 10 ㎍/㎏ i.p.), an inhibitor of guanylate cyclase. 5. The YGJT-induced increase in PAD and MABP were accelerated by pretreatment with IDN but was significantly inhibited by MTB. This results suggested that the mechanism of YGJT is mediated by guanylate cyclase. 6. YGJT inhibited significantly LDH activity in neuronal cells. This results were suggested that YGJT prevented the neuronal death. I thought that YGJT should have improvement of cerebral hemodynamics and inhibitive effect on the brain damage.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.37 no.8B
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• pp.695-705
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• 2012

Due to recent advances in wireless devices and the automotive industry, Vehicular Ad hoc Networks (VANETs) have emerged as a very promising technology for transferring data collected on the road by moving cars. The delivered data may contain emergency information which affects the safety of passengers and drivers as well as the traffic congestion, and the routing protocols have thus a significant impact on the performance of VANETs. In this paper, we study the impact of movement direction of the participating cars which forward data packets on the performance of data delivery and present a new approach which extends the contention-based forwarding (CBF). The proposed reliable CBF (R-CBF) increases the reliability of data deliver on the traffic lights installed roads and reduces the overall propagation delay without routing loops or interruption of data forwarding that may be caused by changes of relative positions of involving cars in routing. Simulation demonstrates that the R-CBF diminishes propagation delay by 38% in comparison to G-SRMB which forwards data to moving cars in the backward direction and eliminates unnecessary retransmissions.

• Journal of Pharmacopuncture
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• v.16 no.4
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• pp.30-35
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• 2013

Objectives: This study was designed to investigate the effects of Calculus Bovis-Fel Uris-Moschus pharmacopuncture (BUM) on the regional cerebral blood flow (rCBF) and the mean arterial blood pressure (MABP) in normal and cerebral ischemic rats and to investigate a possible pathway involved in the effects of BUM. Methods: The changes in the rCBF and the MABP following BUM into Fengfu (GV16) were determined by using a laser-Doppler flow meter and a pressure transducer, respectively. Results: BUM significantly increased the rCBF and decreased the MABP in normal rats in a dose-dependent manner. The effect on the rCBF was significantly inhibited by pretreatment with methylene blue (0.01 mg/kg, intraperitoneal), an inhibitor of guanylate cyclase, but was not affected by pretreatment with indomethacin (1 mg/kg, intraperitoneal), an inhibitor of cyclooxygenase. The BUM-induced decrease of the MABP was changed neither by methylene blue nor by indomethacin pretreatment. In the cerebral ischemic rats, the rCBF was stably increased upon cerebral reperfusion in the BUM group in contrast to the rapid and marked increase in the control group. Conclusion: This study demonstrated that BUM into Fengbu (GV16) increased the rCBF in a dose-dependent manner in the normal state; furthermore, it improved the stability of the rCBF in the ischemic state upon reperfusion. Also, the effects of BUM on the rCBF were attenuated by inhibition of guanylate cyclase, suggesting that the effects involved the guanylate cyclase pathway.

• Journal of Biomedical Engineering Research
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• v.21 no.4
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• pp.339-344
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• 2000

The mucociliary system is one of the most important airway defense mechanisms in human body and impairment of ciliary movement results in various diseases in respiratory tract. In this study, we have developed a system that can measure ciliary movement in vivo and quantified ciliary beat frequency (CBF) through autoregressive (AR) power spectrum. To measure the frequency in vivo, we applied a photoelectric method that was composed of a laser light and a fiber optic probe. Scattered signals are transferred to a PC in which they are displayed on the monitor and its CBF is determined by the AR method in were acquired. For 8 normal subjects, the analyzed CBFs ranged from 5 to 10Hz and its mean was 7.3${\pm}$1.1Hz. This result showed similar aspects to the reported results of CBFs to data. We expect that this result will be applied in various clinical studies such as analysis of CBF changes by drugs or by diseaes.

• Sleep Medicine and Psychophysiology
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• v.1 no.2
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• pp.193-196
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• 1994

Primary depression with cognitive impairment, referred to as depressive pseudodementia, may be mistaken for a progressive degenerative dementia. Recognition of primary depression is clinically important because of its treatability. To differentiate depression from degenerative dementia, author used brain SPECT. By the result the regional cerebral blood flow(rCBF) in elderly depressed patient was decreased in the right frontal cortex. The pattern of rCBF was different from that of dementia which shows decreased rCBF in bifrontal cortex. By using brain SPECT in depressed elderly patient with cognitive impairment, the discrimination from dementia will be more effective and accurate.

• The Journal of Internal Korean Medicine
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• v.20 no.1
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• pp.153-166
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• 1999

SAENGMAEGSAN(SMS) has been used in oriental medicine for many years as a therapeutic agent for cerebral disease. The effect of SMS on the vascular system is not known. The purpose of this study was to determine the effect of SMS on blood pressure, regio-nal cerebral blood flow(rCBF). 1. Blood pressure did not change by SMS in rats. 2. rCBF was increased by SMS in a dose-dependent manner. 3. Pretreatment with propranolol. methylene blue and indomethacin significantly inhibited SMS induced increase in rCBF. These results suggest that SMS causes a diverse response of blood pressure, regional cerebral blood flow (rCBF) and pial arterial diameter. The increase in rCBF is also mediated by prostaglandins. cyclic GMP and adrenergic ${\beta}$receptor.