Browse > Article
http://dx.doi.org/10.4014/jmb.1910.10040

Antioxidant and Anti-Obesity Activities of Polygonum cuspidatum Extract through Alleviation of Lipid Accumulation on 3T3-L1 Adipocytes  

Choi, Da-Hye (Department of Research and Development, Chuncheon Bio-industry Foundation (CBF))
Han, Joon-Hee (Department of Research and Development, Chuncheon Bio-industry Foundation (CBF))
Yu, Keun-Hyung (Department of Research and Development, Chuncheon Bio-industry Foundation (CBF))
Hong, Min (Department of Research and Development, Chuncheon Bio-industry Foundation (CBF))
Lee, Sun-Yeop (Department of Research and Development, Chuncheon Bio-industry Foundation (CBF))
Park, Ka-Hee (Department of Research and Development, Chuncheon Bio-industry Foundation (CBF))
Lee, Soo-Ung (Department of Research and Development, Chuncheon Bio-industry Foundation (CBF))
Kwon, Tae-Hyung (Department of Research and Development, Chuncheon Bio-industry Foundation (CBF))
Publication Information
Journal of Microbiology and Biotechnology / v.30, no.1, 2020 , pp. 21-30 More about this Journal
Abstract
Natural products are widely used due to their various biological activities which include anti-inflammatory, antioxidant, and anti-obesity effects. In this study, we determined the antioxidative and anti-obesity effects of Polygonum cuspidatum 50% ethanol extract (PEE). The antioxidative effect of PEE was evaluated using its radical scavenging activity, total phenolic content, and reducing power. The anti-obesity effect of PEE was investigated using 3T3-L1 adipocytes. The antioxidative activity of PEE was progressively increased in various concentrations, mainly due to the presence of phenolic compounds. PEE also alleviated lipid accumulation on 3T3-L1 adipocytes and downregulated the mRNA and protein production of adipogenesis-related (SREBP-1c, PPARγ, C/EBPα) and lipogenesis-related (aP2, FAS, ACC) markers. Furthermore, we found that the inhibitory effect on lipid accumulation via PEE was caused by the alleviation of NF-κB, p38 MAPK, ERK1/2, and JNK at the protein level. Taken together, our results imply that PEE is a potential antioxidant that can prevent obesity-associated disorders.
Keywords
3T3L1 cells; lipogenesis; adipogenesis; antioxidant; Polygonum cuspidatum;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Kopleman PG. 1994. Causes and consequences of obesity. Med. Int. 22: 385-388.
2 Gr undy SH. 1998. Mutifactorial causation of obesity: implication for prevention. Am. J. Cli. Nutr. 67: 563-572.
3 B arr EL, Cameron AJ, Balkau B, Zimmet PZ, Welborn TA, Tonkin AM, et al. 2010. HOMA insulin sensitivity index and the risk of all-cause mortality and cardiovascular disease events in the general population: the Australian diabetes, obesity and lifestyle study. Diabetologia 53: 79-88.   DOI
4 L iberopoulos EN, Mikhailidis DP, Elisaf MS. 2005. Diagnosis and management of the metabolic syndrome in obesity. Obes. Rev. 6: 283-296.   DOI
5 M okdad AH, Ford ES, Bowman BA, Dietz WH, Vinicor F, Bales VS, et al. 2003. Prevalence of obesity, diabetes, and obesity-related health risk factors, 2001. JAMA 289: 76-79.   DOI
6 Ch o EJ, Rahman A, Kim SW, Beak YM, Hwang HJ, Oh JY, et al. 2008. Chitosan oligosaccharides inhibit adipogenesis in 3T3-L1 adipocyetes. J. Microbiol. Biotechnol. 18: 80-87.
7 K orenblat KM, Fabbrini E, Mohammed BS, Klein S. 2008. Liver, muscle, and adipose tissue insulin action is directly related to intrahepatic triglyceride content in obese subjects. Gastroenterology 134: 1369-1375.   DOI
8 Sorisky A. 1999. From preadipocyte to adipocyte: differentiation-directed signals of insulin from the cell surface to the nucleus. Crit. Rev. Clin. Lab. Sci. 36: 1-34.   DOI
9 Pantoja C, Huff JT, Yamamoto KR. 2008. Glucocorticoid signaling defines a novel commitment state during adipogenesis in vitro. Mol. Biol. Cell. 19: 4032-4041.   DOI
10 Gregoire FM, Smas CM, Sul HS. 1998. Understanding adipocyte differentiation. Physiol. Rev. 78: 783-809.   DOI
11 Ch o SY, Park PJ, Shin HJ, Kim YK, Shin DW, Shin ES, et al. 2007. (-)-Catechin suppresses expression of Kruppel-like factor 7 and increases expression and secretion of adiponectin protein in 3T3-L1 cells. Am J. Physiol. Endocrinol. Metab. 292: E1166-E1172   DOI
12 Jou PC, Ho BY, Hsu YM, Pan TM. 2010. The effect of Monascus secondary polyketide metabolites, monascin and ankaflavin, on adipogenesis and lipogenesis activity in 3T3-L1. J. Agric. Food Chem. 58: 12703-12709.   DOI
13 Th ompson GM, Trainor D, Biswas C, Lacerte C, Berger JP, Kelly LJ. 2004. A high-capacity assay for PPAR-gamma ligand regulation of endogenous aP2 expression in 3T3-L1 cells. Anal. Biochem. 330: 21-28.   DOI
14 B hathena SJ, Velasquez MT. 2002. Beneficial role of dietary phytoestrogens in obesity and diabetes. Am. J. Clin. Nutr. 76: 1191-1201.   DOI
15 Ros en ED, Walkey CJ, Puigserver P, Spiegelman BM. 2000. Transcriptional regulation of adipogenesis. Genes Dev. 14: 1293-1307.
16 Ta bor DE, Kim JB, Spiegelman BM, Edwards PA. 1999. Identification of conserves cis-elements and transcription factors required for sterol-regulated transcription of stearoyl-CoA desaturase 1 and 2. Biol. Chem. 274: 20603-20610.   DOI
17 Kon g CS, Kim JA, Kim SK. 2009. Anti-obesity effect of sulfated glucosamine by AMPK signal pathway in 3T3-L1 adipocytes. Food Chem. Toxicol. 47: 2401-2406.   DOI
18 Johnson GL and Lapadat R. 2002. Mitogen-activated protein kinase pathways mediated by ERK, JNK, and p38 protein kinases. Science 298: 1911-1912.   DOI
19 B aker RG, Hayden MS, Ghosh S. $NF-{\kappa}B$, inflammation, and metabolic disease. 2011. Cell Metab. 13: 11-22.   DOI
20 Lin J, Della-Fera MA, Balie CA. 2005. Green tea polyphenol epigallocatechin gallate inhibits adipogenesis and induces apoptosis in 3T3-L1 adipocytes. Obesity Res. 13: 982-990.   DOI
21 Zh ou Z, Miwa M, Nara K, Wu B, Nakaya H, Lian C, et al. 2003. Patch e stablishment a nd d evelopment of a clonal plant, Polygonym cuspidatum, on Mount Fuji. Mol. Ecol. 12: 1361-1373.   DOI
22 X ing WW, Wu JZ, Jia M, Du J, Zhang H, Qin LP. 2009. Effects of polydatin from Polygonum cuspidatum on lipid profile in hyperlipidemic rabbits. Biomed. Pharmacother. 64: 457-462.
23 A richi H, Kimura Y, Okuda H, Baba K, Kozawa M, Arichi S. 1982. Effects of stilbene components of the roots of Polygonum cuspidatum Sieb. et Zucc. on lipid metabolism. Chem. Pharm. Bull. (Tokyo). 30: 1766-1770.   DOI
24 K uo CH, Chen BY, Liu YC, Chang CMJ, Deng TS, Chen JH, et al. 2014. Optimized ultrasound-assisted extraction of phenolic compounds from Polygonum cuspidatum. Molecules 19: 67-77.   DOI
25 J eong DW, Cho CH, Lee JS, Lee SH, Kim T, Kim DO. 2018. Deastringent peel extracts of persimmon (Diosyros kaki thumb. cv. Cheondo-bansi) p rotect neuronal PC-12 and SHSY5Y cells against oxidative stress. J. Microbiol. Biotechnol. 28: 1094-1104.   DOI
26 Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181: 1199-1200.   DOI
27 Re R, Pellegrini N, Proteggente A, Pannala A, Tang M, Rice-Evans C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biol. Med. 26: 1231-1237.   DOI
28 Oyaizu M. 1986. Studies on products of browning reaction: antioxidant activities of products of browning reaction prepared from glucosamine. Jpn. J. Nutr. 44: 307-315.   DOI
29 Wu YX, Kim YJ, Li S., Yun MC, Yoon JM, Kim JY, et al. Anti-obese effects of mulberry (Morus alba L.) root bark through the inhibition of digestive enzymes and 3T3-L1 adipocyte differentiation. 2015. Korean J. Food Preserv. 22: 27-35.   DOI
30 Kw on TH, Kim TW, Kim CG, Kim TW, Park NH. 2013. Antioxidant activity of various solvent fractions from edible brown alga, Eisenia bicyclcis a nd i ts a ctive compounds. J. Food Sci. 15: C679-C684.
31 Kim NY, Yim TB, Lee HY. 2015. Skin anti-aging activities of bacteriochlorophyll a from photosynthetic bacteria, Rhodobacter sphaeroides. J. Microbiol. Biotechnol. 25: 1589-1598.   DOI
32 Upadhyay S and Dixit M. 2015. Role of p olyphenols a nd other phytochemicals on molecular signaling. Oxid. Med. Cell Longev. 2015: 504253.
33 Tang Y and Tsao R. Phytochemicals in quinoa and amaranth grains and their antioxidant, anti-inflammatory, and potential health beneficial effects: a review. 2017. Mol. Nutr. Food Res. 61: 1600767.   DOI
34 Folin O, Denis W. 1912. On phosphotungstic-phophomolybdic compounds as color reagents. J. Biol. Chem. 12: 239-243.   DOI
35 L efterova MI, Zhang Y, Steger DJ, Schupp M, Schug J, Cristancho A, et al. 2008. PPARgamma and C/EBP factors orchestrate adipocyte biology via adjacent binding on a genome-wide scale. Genes Dev. 22: 2941-2952.   DOI
36 Izuegbuna O, Otunola G, Bradley G. 2019. Chemical composition, antioxidant, anti-inflammatory, and cytotoxic activities of Opuntia stricta cladodes. PLoS One 14: e02209682.
37 M ayouf N, Charef N, Saoudi S, Baghiani A, Khennouf S, Arrar L. 2019. Antioxidant and anti-inflammatory effect of Asphodelus microcarpus methanolic extracts. J. Ethnopharmacol. 239: doi.org/10.1016/j.jep.2019.111914.   DOI
38 Wa ng Y, Lee PS, Chen YF, Ho CT, Pan MH. 2016. Suppression of adipogenesis by 5-hydroxy-3,6,7,8,3',4'-hexamethoxyflavone from orange peel in 3T3-L1 cells. J. Med. Food. 19: 830-835.   DOI
39 Zebis ch K, Voigt V, Wabitsch M, Brandsch M. 2012. Protocol for effective differentiation of 3T3-L1 cells to adipocytes. Anal. Biochem. 425: 88-90.   DOI
40 Moseti D, Regassa A, Kim WK. 2016. Molecular regulation of adipogenesis and potential anti-adipogenic bioactive molecules. Int. J. Mol. Sci. 17: 124.   DOI
41 Munshi A, Ramesh R. 2013. Mitogen-activated protein kinases and their role in radiation response. Genes Cancer 4: 401-408.   DOI
42 L i KK, Liu CL, Shiu HT, Wong HL, Siu WS, Zhang C, et al. 2016. Cocoa tea (Camellia ptilophylla) water extract inhibits adipocyte differentiation in mouse 3T3-L1 preadipocytes. Sci. Rep. 1: 20172.
43 Zhang T, Yamamoto N, Yamashita Y, Ashida H. 2014. The chalcones cardamonin and flavokawain B inhibit the differentiation of preadipocytes to adipocytes by activating ERK. Arch. Biochem. Biophys. 15: 44-54.
44 Zh ang C, Teng L, Shi Y, Jin J, Xue Y, Shang K, et al. 2002. Effect of emodin on proliferation and differentiation of 3T3-L1 preadipocyte and FAS activity. Chin. Med. J. 115: 1035-1038.   DOI
45 Song P, Kim JH, Ghim J, Yoon JH, Lee A, Kwon Y, et al. 2013. Emodin regulates glucose utilization by activating AMP-activated protein kinase. J. Biol. Chem. 288: 5732-5742.   DOI
46 Chen Z, Zhang L, Yi J, Yang Z, Zhang Z, Li Z. 2012. Promotion of adiponectin multimerization by emodin: a novel AMPK activator with $PPAR{\gamma}$-agonist activity. J. Biol. Chem. 113: 3547-3558.