• Proceedings of the KSME Conference
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• 2000.04b
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• pp.270-275
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• 2000

In this study, heat transfer and Pressure drop characteristics for R-718 in the plate and shell heat exchanger (P&SHE) investigated experimentally. The plates are circular and welded into a stack which fits into a cylindrical shell in P&SHE. Although apparently very different from rectangular the compact brazed plate heat exchanger (CBE), the underlying flow passage structure through the P&SHE is the same as in the CBE. The R-718 between plate side and shell side was performed a counterflow heat exchange. Heat transfer characteristic of R-718 were measured for turbulent flow in P&SHE by using wilson plot technique. Heat transfer experiment Ivas performed in the $200{\leq}Re{\leq}500$ regime and Pressure drop experiment was performed in the $150{\leq}Re{\leq}1600$ regime. The purpose of this study is to investigate heat transfer and friction factor correlations for R-718 in P&SHE and to offer fundamental data for experiment

• Korean Medical Education Review
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• v.19 no.1
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• pp.25-35
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• 2017

Recently, there has been active reformation of higher education. This trend has resulted in competency-based education (CBE) in many universities around the world, and dentistry education is no exception. However, it is necessary to keep in mind that CBE is both attractive and has its limitations. In particular, higher education is facing the obstacle of preparing students to survive in a supercomplex world in which nothing can be taken for granted. In addition, the frame of understanding and action lacks stability. In these circumstances, competency-based dentistry curriculum (CBDC) needs to be reestablished to deal with the changes and challenges of a supercomplex world. The purpose of this study is to explore alternatives to current CBDC practices, specifically based on an 'ontological approach.' To achieve this purpose, the importance of the ontological approach in the development of higher education curriculum in the future was examined. Then, the actual status and characteristics of CBDC in the present situation were investigated. Lastly, the development of CBCD based on an ontological approach in dentistry education was suggested.

• International Journal of Stem Cells
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• v.16 no.2
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• pp.234-243
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• 2023

The recent advances in human pluripotent stem cells (hPSCs) enable to precisely edit the desired bases in hPSCs to be used for the establishment of isogenic disease models and autologous ex vivo cell therapy. The knock-in approach based on the homologous directed repair with Cas9 endonuclease, causing DNA double-strand breaks (DSBs), produces not only insertion and deletion (indel) mutations but also deleterious large deletions. On the contrary, due to the lack of Cas9 endonuclease activity, base editors (BEs) such as adenine base editor (ABE) and cytosine base editor (CBE) allow precise base substitution by conjugated deaminase activity, free from DSB formation. Despite the limitation of BEs in transition substitution, precise base editing by BEs with no massive off-targets is suggested to be a prospective alternative in hPSCs for clinical applications. Considering the unique cellular characteristics of hPSCs, a few points should be considered. Herein, we describe an updated and optimized protocol for base editing in hPSCs. We also describe an improved methodology for CBE-based C to T substitutions, which are generally lower than A to G substitutions in hPSCs.

• Korean Journal of Food Science and Technology
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• v.52 no.2
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• pp.138-143
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• 2020

Black chokeberry (Aronia melanocarpa) has been suggested to exert antioxidant and anti-inflammatory effects due to its high polyphenol content. However, the mechanisms underlying the effects of black chokeberry on the alterations of nuclear factor E2-related factor 2 (NRF2) and nuclear factor κB (NF-κB) in macrophages have not been thoroughly studied. In this study, we investigated the protective effects of polyphenol-rich black chokeberry extract (CBE) against lipopolysaccharide (LPS)-induced oxidative stress and inflammation in RAW 264.7 macrophages. CBE significantly attenuated the increase of cellular reactive oxygen species (ROS) levels and the nuclear translocation of NRF-2 in LPS-stimulated macrophages. The mRNA abundances of Nrf2 and its downstream antioxidant genes were significantly decreased in LPS-stimulated macrophages. The LPS-induced mRNA expression of proinflammatory cytokines was significantly inhibited by reducing the nuclear translocation of NF-κB by CBE. These data suggest that black chokeberry may be used for the prevention of oxidative stress and inflammation-associated disease.

• Journal of Microbiology and Biotechnology
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• v.31 no.7
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• pp.942-948
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• 2021

Canine influenza virus (CIV) induces acute respiratory disease in dogs. In this study, we aimed to determine the signaling pathways leading to the induction of IFN-β in a canine respiratory epithelial cell line (KU-CBE) infected with the H3N2 subtype of CIV. Small interfering RNAs (siRNAs) specific to pattern recognition receptors (PRRs) and transcription factors were used to block the IFN-β induction signals in H3N2 CIV-infected KU-CBE cells. Among the PRRs, only the TLR3 and RIG-I expression levels significantly (p < 0.001) increased in CIV-infected cells. Following transfection with siRNA specific to TLR3 (siTLR3) or RIG-I (siRIG-I), the mRNA expression levels of IFN-β significantly (p < 0.001) decreased, and the protein expression of IFN-β also decreased in infected cells. In addition, co-transfection with both siTLR3 and siRIG-I significantly reduced IRF3 (p < 0.001) and IFN-β (p < 0.001) mRNA levels. Moreover, the protein concentration of IFN-β was significantly (p < 0.01) lower in cells co-transfected with both siTLR3 and siRIG-I than in cells transfected with either siTLR3 or siRIG-I alone. Also, the antiviral protein MX1 was only expressed in KU-CBE cells infected with CIV or treated with IFN-β or IFN-α. Thus, we speculate that IFN-β further induces MX1 expression, which might suppress CIV replication. Taken together, these data indicate that TLR3 and RIG-I synergistically induce IFN-β expression via the activation of IRF3, and the produced IFN-β further induces the production of MX1, which would suppress CIV replication in CIV-infected cells.

• Microbiology and Biotechnology Letters
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• v.18 no.5
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• pp.460-465
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• 1990

Regeneration of protoplast was effective by preincubating spore suspension containing 30$\mu g$/ml of 2-DG for 4 hours, and CBE medium containing casamino acid, bovine serum albumin, ergosterol and myoinositol was found to be more efficient than any other regeneration medium tested in this experiment. The regeneration frequency was about 30%. Optimal conditions for conidial protoplast fusion were obtained by treatment of protoplasts with 10 mM $CaCl_2$ and 30% polyethylene glycol 4000 (pH 7.5) as fusogenic agent at $37^{\circ}C$ for 10 minutes. The fusion frequency was $8.2\times 10^{-4}$. The higher productivity of enzyme of fusant FWN-56 was achived: 2.3-fold for CMCase, 1.5-fold for avicelase, 1.8-fold for $\beta$-glucosidase and 2.5-fold for xylanase compared to that obtained in two parental strains. The genetic stability of fusant after maintenance on minimal medium for more than 4 weeks was high because segregant rate was below 1%. The conidial DNA content of fusant was 1.4-1.6 times higher than that of the parental strains, The nucleus size of fusants were also higher than that of each parental strains.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.1
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• pp.267-274
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• 2013

The impact of income and education level on the clinical and pathologic characteristics, implementation of clinical breast examination (CBE), and treatment patterns of a small population of Chinese female breast cancer patients was studeied in order to provide a theoretical basis and statistical reference for further nationwide research. We included 484 pathologically confirmed female primary breast cancer inpatients of the First Affiliated Hospital of Medical College of Xi'an Jiaotong University from February 2003 to January 2004. All cases were reviewed and relevant information was collected using a designed case report form (CRF). Chisquare tests, rank-sum tests, and Fisher's exact tests were used in the analysis. Our analysis showed that: (1) women in different occupation groups had significant differences in tumor size, pre-operative mammography, surgical options, post-operative estrogen receptor (ER), progestin receptor (PR) and human epidermal growth factor receptor 2 (Her2) status, and post-operative radiotherapy and chemotherapy (P < 0.05); and (2) women with different education levels had statistically significant differences in tumor size, post-operative ER, PR and Her2 status, and post-operative chemotherapy, radiotherapy, and endocrine therapy (P < 0.05). In Xi'an, China, women in low-income occupations or with low education levels are more likely to have advanced tumor stages at presentation, lower implementation rate of clinical breast examination, and less treatment.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.3
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• pp.1181-1184
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• 2015

Background: Accurate measurement of breast mass size is fundamental for treatment planning. We evaluated performance of BreastLight apparatus in detection breast of masses with this in mind. Materials and Methods: From July 2011 to September 2013, a total of 500 women referred to mammography unit in Yazd, Iran for screening were recruited to this study. Performance of BreastLight in detection breast masses regard their sizeing, measured with clinical breast examination (CBE), mammography and sonography, was assessed. Sonographic and mammography examinations were performed according to breast density among women in two groups of women younger (n=105) and older (n=395) than 30 years. Size correlations were performed using Spearman rho analysis. Differences between mass size as assessed with the different methods (mammography, sonography, and clinical examination) and the BreastLight detection were analyzed using $X^2$-trend test. Results: Performance of the BreastLight in detection of lesions smaller than or equal to 1 cm assessed by CBE, mammography and sonography was 4.4%,7.7% and 12.5% and for masses larger than 4 cm was 65%, 100% and 57.1%, respectively. The performance of BreastLight in detection was significantly increased with larger masses (p<0.001). Conclusions: We conclude that clinical measurement of breast cancer size is as accurate as that from mammography or ultrasound. Accuracy can be improved by the use of a simple formula of both clinical and mammographic measurements.

• Journal of KIISE:Computing Practices and Letters
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• v.14 no.3
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• pp.331-335
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• 2008

In this paper, we parallelized a feature point extraction algorithm to reduce panorama image generation time in multi-core environment. While we compose a panorama image with several images, the step to extract feature points of each picture is needed to find overlapped region of pictures. To perform rapidly feature extraction stage which requires much calculation, we developed a parallel algorithm to extract feature points and examined the performance using CBE(Cell Broadband Engine) which is asymmetric multi-core architecture. As a result of the exam, the algorithm we proposed has a property of linear scalability-the performance is increased in proportion the number of processors utilized. In this paper, we will suggest how Image processing operation can make high performance result in multi-core environment.

• Journal of the Korean Mathematical Society
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• v.50 no.1
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• pp.81-93
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• 2013

We consider a distributed optimal flux control problem: finding the potential of which gradient approximates the target vector field under an elliptic constraint. Introducing the Lagrange multiplier and a change of variables the Euler-Lagrange equation turns into a coupled equation of an elliptic equation and a reaction diffusion equation. The change of variables reduces iteration steps dramatically when the Gauss-Seidel iteration is considered as a solution method. For the elliptic equation solver we consider the Cell Boundary Element (CBE) method, which is the finite element type flux preserving methods.