• Journal of Microbiology and Biotechnology
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• v.25 no.1
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• pp.119-126
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• 2015

Among the various human growth factors, epidermal growth factor (hEGF, consisting of 53 amino acids) has various effects on cell regeneration, stimulation of proliferation, migration of keratinocytes, formation of granulation tissues, and stimulation of fibroblast motility, which are important for wound healing. Owing to their multiple activities, EGFs are used as pharmaceutical and cosmetic agents. However, their low productivity, limited target specificity, and short half-life inhibit their application as therapeutic agents. To overcome these obstacles, we fused the collagen-binding domain (CBD) of Vibrio mimicus metalloprotease to EGF protein. About 18 or 12 amino acids (aa) (of the 33 total amino acids), which were essential for collagen-binding activity, were combined with the N- and C-termini of EGF. We constructed, expressed, and purified EGF (53 aa)-CBD (18 aa), EGF (53 aa)-CBD (12 aa), CBD (18 aa)-EGF (53 aa), and CBD (12 aa)-EGF (53 aa). These purified recombinant proteins increased the numbers of cells in treated specimens compared with non-treated specimens and control hEGF samples. The collagen-binding activities were also evaluated. Furthermore, CBD-hybridized hEGF induced phosphorylation of the EGF receptor. These results suggested that these fusion proteins could be applicable as small therapeutic agents in wound tissue healing.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• v.23 no.6
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• pp.531-538
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• 2020

Purpose: Our aim was the longitudinal assessment of cyst size in fetuses with biliary cystic malformation (BCM) to explore its etiology and the possibility of antenatal differentiation between biliary atresia (BA) and congenital biliary dilatation (CBD). Methods: We conducted a retrospective review of all patients diagnosed antenatally with BCM from 1994 to 2014 at our institutions. Results: The study cohort comprised of three patients with BA and six with CBD. There were no significant differences in the gestational age and cyst size at the first detection of BCM between the two groups. In fetuses with CBD, the cyst size steadily increased as the gestational age advanced, while it fluctuated around 1.5 cm and remained below 2.1 cm in those with BA. However, the ratio of cystic area to fetal trunk area was approximately constant due to linear fetal growth in fetuses with CBD. Conclusion: Fetuses with BCM <2.1 cm in the late gestation period were more likely to have BA than CBD. Our observation of cyst enlargement with advancing gestational age in the CBD group was attributed solely to fetal growth. Biliary dilatation in fetuses with CBD and BA might be completed at the onset of BCM.

• Journal of Physiology & Pathology in Korean Medicine
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• v.22 no.1
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• pp.120-125
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• 2008

This experiment was designed to investigate the effect of the CBD and SJT extract on protecting microglia and inhibiting acetylcholinesterase and oxidants. The effects of the CBD and SJT extract on cell death of BV2 microglial cell line treated by $IFN-{\gamma}$ ; expression of NO, ROS in BV2 microglial cell line treated by lipopolysaccharide(LPS) ; AChE activity in PC-12 cell treated by NGF were investigated, respectively. The CBD and SJT extract significantly increased cell viability in BV2 microglial cell line treated with $IFN-{\gamma}$. The CBD and SJT extract suppressed the NO and ROS production in BV2 microglial cell line treated by LPS. The CBD and SJT extract groups also showed inhibition of AChE activity in PC-12 cell line. According to the above result, it is suggested that the CBD and SJT extract might be usefully applied for prevention and treatment of Alzheimer's disease.

• The KIPS Transactions:PartD
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• v.11D no.1
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• pp.133-142
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• 2004

The next generation of web applications will need to be larger, more complex, and flexible Agent-oriented systems have great potential for these e-commerce applications. Agents can dynamically discover and compose e-services and mediate interactions. Development of software agents with CBD (Component Based Development) has proved to be successful in increasing speed to market of development Projects, lowering the development cost and providing better qualify. In this thesis, we propose a systemic development process for software agents using component and UML (Unified Modeling Language). We suggest a etA (e-business Agent) CBD reference architecture for layer the related components through identification and classification of general agent and e-business agent. We also propose the ebA-CBD process that is a guideline to consider the best features of existing agent oriented software engineering methodologies, while grounding agent-oriented concepts in the same underlying semantic framework used by UML. We first developed the agent components specification and modeled it with Goal, Role, Interaction, and Architecture Model. Based on this, we developed e-CPIMAS (e-Commerce Product Information Mailing Agent System) as a case study that provides the product information's mailing service according to proposed process formality. We finally describe how these concepts may assist in increasing the efficiency reusability, productivity and quality to develop the business application and e-business agent.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.48 no.4
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• pp.313-319
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• 2022

In this study, the stability of cream containing 1% of cannabidiol (CBD) with more than 99% extracted from domestic Cannabis sativa was evaluated. The pH, hardness, content, and chromaticity were measured at intervals of 2 weeks under storage conditions for each temperature for 12 weeks (4 ℃, 25 ℃, 37 ℃, and 45 ℃). The pH of the experimental group containing 1% of CBD tended to decrease more than that of the control group. As a result of measuring the change in the content of CBD in the cream under each temperature for 12 weeks using HPLC, the difference in the amount of decrement according to temperature was confirmed. It was confirmed that as a result of measuring the chromaticity using color difference meter, the yellowness of the cream containing CBD increased according to the increase by the storage period and temperature compared to the control group. From the above results, it was confirmed that the content decrease and color change according to the temperature of the pH 5 ~ 6 cream formulation containing CBD. When CBD is applied to medicine and cosmetic formulations in the future, it is considered that research and development should be conducted in consideration of the change in the quantity and physicochemical quality of CBD under the temperature.

• Journal of the Korea Society for Simulation
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• v.12 no.3
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• pp.95-102
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• 2003

Lately CBD methodologies like as CBD96, Catalysis, Fusion, and SCIPIO have been introduced. These methodologies has their own proprietary component reference model. Using proprietary reference model falls interoperability among methodologies. Furthermore it can cause confusion and difficulty for component developers, In this paper, we propose a integrated component meta-model for support consistency and interoperability among component designs. Also, we compare our proposed meta model to existing component reference model by using component's characteristics. We expect that it is easy to add new meta model elements and extends meta-model by using integrated component meta-model.

• Journal of Veterinary Science
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• v.21 no.3
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• pp.39.1-39.15
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• 2020

Background: There are various Helicobacter species colonizing the stomachs of animals. Although Helicobacter species usually cause asymptomatic infection in the hosts, clinical signs can occur due to gastritis associated with Helicobacter in animals. Among them, Helicobacter pylori is strongly associated with chronic gastritis, gastric ulcers, and gastric cancers. As the standard therapies used to treat H. pylori have proven insufficient, alternative options are needed to prevent and eradicate the diseases associated with this bacterium. Cheonwangbosim-dan (CBD), a traditional herbal formula that is popular in East Asia, has been commonly used for arterial or auricular flutter, neurosis, insomnia, and cardiac malfunction-induced disease. Objectives: The present study investigated the antimicrobial effect of CBD on H. pylori-infected human gastric carcinoma AGS cells and model mice. Methods: AGS cells were infected with H. pylori and treated with a variety of concentrations of CBD or antibiotics. Mice were given 3 oral inoculations with H. pylori and then dosed with CBD (100 or 500 mg/kg) for 4 weeks or with standard antibiotics for 1 week. One week after the last treatment, gastric samples were collected and examined by histopathological analysis, real-time quantitative polymerase chain reaction, and immunoblotting. Results: Our results showed that CBD treatment of AGS cells significantly reduced the H. pylori-induced elevations of interleukin-8, inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2). In the animal model, CBD treatment inhibited the colonization of H. pylori and the levels of malondialdehyde, inflammation, proinflammatory cytokines, iNOS, and COX-2 in gastric tissues. CBD also decreased the phosphorylation levels of p38 mitogen-activated protein kinase family. Conclusions: This study suggests that CBD might be a prospective candidate for treating H. pylori-induced gastric injury.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2008.06a
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• pp.903-904
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• 2008

• Proceedings of the Korea Information Processing Society Conference
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• 2005.05a
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• pp.271-274
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• 2005

소프트웨어 제품의 활용 범위가 늘어나면서 품질의 중요성이 부각되고 있다. 이에 따라 ISO/IEC 9126 품질모델과 ISO/IEC 14598 소프트웨어 제품 평가모델에 대한 표준이 발표되었다. 하지만, 대부분 소프트웨어 개발 사업에서는 결함사항 위주로 품질관리를 하고 있으며 표준모델은 미적용 되고 있는 실정이다. 따라서, 본 논문에서는 국방 CBD 방법론을 기반으로 표준 소프트웨어 품질모델 및 제품 평가모델을 이용한 품질평가 프로세스를 제시하고자 한다. 이를 위해 국방 CBD 방법론 산출물의 품질평가 모델과 품질평가 프로세스를 정의하고 평가활동 단계별 산출물을 제시한다. 또한 소규모 시범사업에 적용을 통하여 제안된 모델의 적절성을 검증하였다. 향후 본 연구의 결과가 국방 CBD 방법론 기반 소프트웨어의 품질관리 활동에 적용이 기대된다.

• Proceedings of the Korean Information Science Society Conference
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• 2005.11b
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• pp.334-336
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• 2005

유비쿼터스 시대에 진입하면서 임베디드 소프트웨어의 개발에 대한 관심이 높아지고 있다. 국방 분야에서는 국방무기 및 비무기 내장형 소프트웨어 관리지침과 공군에서 운영되는 항공무기 소프트웨어 개발 프로세스 등이 운용되고 있으며, 국방 CBD 방법론은 주로 응용체계 개발을 위한 컴포넌트 기반의 구체적인 방법론이다. 이러한 국방 CBD 방법론을 임베디드 소프트웨어 개발에 적용가능 여부는 발표되지 않았다. 따라서 본 논문에서는 임베디드 소프트웨어개발을 위한 국방 CBD 프로세스 테일러링 방안을 고찰하고자 한다. 이를 위하여 국방 무기 및 비무기 내장형 소프트웨어 관리지침에 근거한 조정, 임베디드 소프트웨어 개발 방식에 따른 조정 컴포넌트 및 객체 지향기술, 그리고 UML 기술 적용으로 구분하여 테일러링 방안을 제시한다. 그 결과 국방 CBD 방법론의 적용 범위의 확대 및 개발 프로세스의 표준화에 기여할 것으로 기대된다.