Journal of Veterinary Science

  • 제21권3호
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  • Pages.39.1-39.15
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  • 2020
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  • 1229-845X(pISSN)
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  • 1976-555X(eISSN)
대한수의학회 (The Korean Society of Veterinary Science)
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Anti-microbial and anti-inflammatory effects of Cheonwangbosim-dan against Helicobacter pylori-induced gastritis

  • Park, Hee-Seon (Department of Veterinary Pathology, College of Veterinary Medicine, Chungnam National University) ;
  • Jeong, Hye-Yun (Department of Veterinary Pathology, College of Veterinary Medicine, Chungnam National University) ;
  • Kim, Young-Suk (Department of Veterinary Pathology, College of Veterinary Medicine, Chungnam National University) ;
  • Seo, Chang-Seob (Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Ha, Hyekyung (Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Kwon, Hyo-Jung (Department of Veterinary Pathology, College of Veterinary Medicine, Chungnam National University)
  • 투고 : 2019.10.10
  • 심사 : 2020.01.26
  • 발행 : 2020.05.31
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초록

Background: There are various Helicobacter species colonizing the stomachs of animals. Although Helicobacter species usually cause asymptomatic infection in the hosts, clinical signs can occur due to gastritis associated with Helicobacter in animals. Among them, Helicobacter pylori is strongly associated with chronic gastritis, gastric ulcers, and gastric cancers. As the standard therapies used to treat H. pylori have proven insufficient, alternative options are needed to prevent and eradicate the diseases associated with this bacterium. Cheonwangbosim-dan (CBD), a traditional herbal formula that is popular in East Asia, has been commonly used for arterial or auricular flutter, neurosis, insomnia, and cardiac malfunction-induced disease. Objectives: The present study investigated the antimicrobial effect of CBD on H. pylori-infected human gastric carcinoma AGS cells and model mice. Methods: AGS cells were infected with H. pylori and treated with a variety of concentrations of CBD or antibiotics. Mice were given 3 oral inoculations with H. pylori and then dosed with CBD (100 or 500 mg/kg) for 4 weeks or with standard antibiotics for 1 week. One week after the last treatment, gastric samples were collected and examined by histopathological analysis, real-time quantitative polymerase chain reaction, and immunoblotting. Results: Our results showed that CBD treatment of AGS cells significantly reduced the H. pylori-induced elevations of interleukin-8, inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2). In the animal model, CBD treatment inhibited the colonization of H. pylori and the levels of malondialdehyde, inflammation, proinflammatory cytokines, iNOS, and COX-2 in gastric tissues. CBD also decreased the phosphorylation levels of p38 mitogen-activated protein kinase family. Conclusions: This study suggests that CBD might be a prospective candidate for treating H. pylori-induced gastric injury.

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과제정보

The present research was supported by a grant from the Korea Institute of Oriental Medicine (KIOM; Evaluation of Herb-Drug Interactions, K16252).

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