• 한국생물공학회:학술대회논문집
• /
• 한국생물공학회 2004년도 학술대회지
• /
• pp.1-20
• /
• 2004

Polychlorinated biphenyls (PCBs), one a group of persistent and widespread environmental pollutants, have been considered to be involved in immunotoxicity, carcinogenesis, and apoptosis. However, the toxic effects and physical properties of a PCB congener are dependent on the structure. In the present study, we investigate the toxic effects and action mechanisms of PCBs In cells. Among the various congeners tested, 2,2',4,6,6'-PeCB-pentachlorobiphenyl (PeCB), a highly ortho-substituted congener having negligible binding affinity for aryl hydrocarbon receptor (AhR), caused the most potent toxicity and specific effects in several cell types. 2,2',4,6,6'-PeCB induced apoptotic cell death of human monocytic cells, suggesting that PCB-induced apoptosis may be linked to immunotoxicity. In addition, 2,2',4,6,6'-PeCB induced mitotic arrest by interfering with mitotic spindle assembly in NIH3T3 fibroblasts, followed by genetic instability which triggers p53 activation. Which suggests that 2,2',4,6,6'-PeCB may be involved in cancer development by causing genetic instability through mitotic spindle damage. On the other hand, 2,2',4,6,6'-PeCB increased cyclooxygenase-2 (COX-2) involved in cell survival through ERK1/2 MAPK and p53 in Rat-1 fibroblasts and mouse embryonic fibroblasts, triggering compensatory mechanism for abating its toxicity. Taken together, these results demonstrate that PCB congeners of different structure have distinct mechanism of action and 2,2',4,6,6'-PeCB causes several toxicity as well as compensatory mechanism in cells.

• International Journal of Industrial Entomology and Biomaterials
• /
• 제8권1호
• /
• pp.89-93
• /
• 2004

Expression of a fusion protein between B. thuringiensis crystal protein, Cry1Ac1 and a scorpion insect toxin (AaIT, Androctonus australis Hector insect toxin) in acrystalliferous B. thuringiensis strain (Cry-B strain) was examined. The cry 1Ac1 gene was cloned in B. thuringiensis-E coli shuttle vector, pHT3101, under the control of the native cry 1Ac1 gene promoter (pProAc) and a gene encoding AaIT was inserted in XhoI site in the middle of the cry 1Ac1 gene (pProAc-ScoR). B. thuringiensis Cry-B strain carrying pProAc-ScoR (PyoAc-ScoR/CB) produced an inclusion body of irregular shape and the expressed fusion protein is approximately 65 kDa in size. Sporulated cells and spore-crystal mixtures of ProAc-ScoR/CB had insecticidal activity against Plutella xylostella larvae, showing $LT_50$ of ProAc-ScoR/CB (22.59 hrs) lower than that of ProAc/CB (30.06 hrs) at $1{\times}{10^7} {CEU/cm^2}$. These results suggest that the fusion protein including a B. thuringiensis crystal protein and an AaIT may be functionally expressed in B. thupingiensis. Moreover, we verified the additive toxicity of AaIT, which is a new feasible candidate for insect control.

• 대한치과기공학회지
• /
• 제10권1호
• /
• pp.47-54
• /
• 1988

The purpose of this study is to evaluate the castability of Ni-Cr alloys for crown and bridge. New young, Hi-Crown and CB-80 which are widely used in dental laboratory were chosen, casting and increased the temperature of furnace 100$^{\circ}C$ of each 7 grades from 600$^{\circ}C$ to 1200$^{\circ}C$. The results are as follows : 1. CB-80 is the most prominent castability among the three Ni-Cr alloys which are Hi-Crown, New-young and Hi-Cr. 2. The good burning temperatures of casting for CB-8p are 900$^{\circ}C$, 100$^{\circ}C$ and 1100$^{\circ}C$ which are obtained four complate casting body of five former patterns. 3. The most proper burning temperature for three Ni-Cr alloys is 900$^{\circ}C$.

• Journal of Microbiology and Biotechnology
• /
• 제23권7호
• /
• pp.974-983
• /
• 2013

Bacillus amyloliquefaciens CB1 was isolated from cheonggukjang, a Korean fermented soy food. B. amyloliquefaciens CB1 secretes proteases with fibrinolytic activities. A gene homologous to aprE of Bacillus subtilis, aprECB1, was cloned from B. amyloliquefaciens CB1, and DNA sequencing showed that aprECB1 can encode a prepro-type serine protease consisting of 382 amino acids. When aprECB1 was introduced into B. subtilis WB600 using an E. coli-Bacillus shuttle vector, pHY300PLK, transformants showed fibrinolytic activity and produced a 28 kDa protein, the size expected for the mature enzyme. The 28 kDa fibrinolytic enzyme was purified from the culture supernatant of B. subtilis WB600 transformant. AprECB1 was completely inhibited by phenylmethylsulfonyl fluoride and almost completely inhibited by EDTA and EGTA, indicating that it is a serine metalloprotease. AprECB1 exhibited the highest specificity for N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide, a known substrate for ${\alpha}$-chymotrypsin. $A{\alpha}$ and $B{\beta}$ chains of fibrinogen were quickly degraded by AprECB1, but the ${\gamma}$-chain was resistant.

• 미생물학회지
• /
• 제31권2호
• /
• pp.129-134
• /
• 1993

4-Chlorobiphenyl(4CB) 과 biphenyl 을 분해하는 Pseudomoas sp. DJ-12 의 pcbAB 는 분해초기 단계에 작용하는 4-chlorobiphenyl dioxygenase 와 dihydrodiol dehydrogenase 효소를 생산하는 유전자들이다. 이 유전자를 E. coli XL1-Blue 에 플로닝하여 CU101 형질전환체를 얻었다. CU101 의 pCU101 재조합 plasmid 에 클로닝된 pcbAB 유전자는 크기가 약 2.2 kb 이고 3 개의 Hind III 제한효소 위치가 있었으며, 독자적인 promoter 를 가지고 있었다. CU101 에 대하여 biphenyl 을 기질로 하여 생성된 대사산물을 resting cell assay 를 한 결과 2, 3-dihydroxybiphenyl 이 검출되어 pcbAB 유전자들이 E. coli 에서 잔 발현된다는 것을 의미하였다. 그러나 dechlorination 작용은 pcbAB 유전자와 관계없이 4AB 의 개환과정 후 생성된 4-chlorobenzoate 에서 일어나는 것으로 해석된다.

• Molecules and Cells
• /
• 제39권10호
• /
• pp.728-733
• /
• 2016

Mesenchymal stem cells (MSCs) effectively reduce airway inflammation and regenerate the alveolus in cigarette- and elastase-induced chronic obstructive pulmonary disease (COPD) animal models. The effects of stem cells are thought to be paracrine and immune-modulatory because very few stem cells remain in the lung one day after their systemic injection, which has been demonstrated previously. In this report, we analyzed the gene expression profiles to compare mouse lungs with chronic exposure to cigarette smoke with non-exposed lungs. Gene expression profiling was also conducted in a mouse lung tissue with chronic exposure to cigarette smoke following the systemic injection of human cord blood-derived mesenchymal stem cells (hCB-MSCs). Globally, 834 genes were differentially expressed after systemic injection of hCB-MSCs. Seven and 21 genes, respectively, were up-and downregulated on days 1, 4, and 14 after HCB-MSC injection. The Hbb and Hba, genes with oxygen transport and antioxidant functions, were increased on days 1 and 14. A serine protease inhibitor was also increased at a similar time point after injection of hCB-MSCs. Gene Ontology analysis indicated that the levels of genes related to immune responses, metabolic processes, and blood vessel development were altered, indicating host responses after hCB-MSC injection. These gene expression changes suggest that MSCs induce a regeneration mechanism against COPD induced by cigarette smoke. These analyses provide basic data for understanding the regeneration mechanisms promoted by hCB-MSCs in cigarette smoke-induced COPD.

• 수산해양교육연구
• /
• 제29권2호
• /
• pp.537-543
• /
• 2017

The purpose of this research is to investigate the effects of different shoe-cleat position on pedalling performance. Four male elite triathletes(age: $22.00{\times}2.16years$, height: $175.12{\pm}8.06cm$, weight: $71.20{\pm}7.89kg$, body fat: $16.62{\pm}3.56%$) and three female elite triathletes(age: $20.00{\pm}1years$, height: $158.40{\pm}2.42cm$, weight: $51.30{\pm}3.89kg$, body fat: $19.26{\pm}2.28%$) participated in 10km time trial and 30sec time trial pedaling tests with the individual time trials based on different shoe-cleat position(cleat front: CF, cleat back: CB). The subjects performed one trial with each type of shoe-cleat position. Maximal power output and average speed were not significantly different during 30s time trial in CF compared with CB. Average power, RPM, and HR were not significantly different during 10k time trial in CF compared with CB. Split time in 1km, 5km, 9km were significantly reduced during 10k time trial in CB compared with CF. We conclude that there was performance advantage in CB using shoe-cleat back position in comparison with CF using shoe-cleat front position.

• 한국식품저장유통학회지
• /
• 제20권6호
• /
• pp.760-765
• /
• 2013

신선편이 연근의 갈변도를 분석하는 방법으로써 이미지 분석법에 대한 가능성에 대해서 연구하였다. 우선 신선편이 연근의 갈변 저해 처리를 위해서 $50^{\circ}C$의 증류수에 5분간 블랜칭 처리(DB), $50^{\circ}C$의 1% ascorbic acid에 5분간 블랜칭 처리(AB), $50^{\circ}C$의 1% citric acid에 5분간 블랜칭 처리(CB)하고, 절단 후 아무 처리도 하지 않은 것을 대조구로 하였다. 갈변 저해 처리 후 0.04 mm의 polyethylene film($25cm{\times}30cm$)에 열 접합 포장 후 $4^{\circ}C$에서 9일 동안 저장하면서 관능평가, 갈변도, polyphenol oxidase 활성을 분석하였다. 연근의 갈변도 분석은 사진 자료를 바탕으로 3가지 컬러 모델을 사용하였는데, 우선 RGB와 CIE $L^*a^*b^*$ 컬러 모델에서 각각의 색 지표를 분석하고 HSV 컬러 모델을 이용하여 갈변면적을 분석하였다. 관능 평가에서 AB와 CB 처리구가 저장 9일째까지 갈변 현상이 가장 많이 억제된 것으로 나타났고, RGB 컬러 모델에서 R, G, B 값이 모든 처리구에서 감소하는 것을 확인하였으며, 특히 AB와 CB 처리구에서 감소하는 정도가 가장 적은 것으로 나타났다. CIE $L^*a^*b^*$와 HSV 컬러 모델에서는 저장 기간이 지남에 따라서 $L^*$값은 감소하고 $a^*$, $b^*$ 값과 갈변 면적은 증가하는 것으로 확인되었는데 AB와 CB 처리구에서 값의 변화 정도가 가장 낮은 것을 확인 할 수 있었다. Polyphenol oxidase 활성 분석에서도 모든 저장 기간 동안 AB와 CB 처리구에서 효소의 활성이 가장 낮게 나타났으며, 위의 결과들을 토대로 유기산과 블랜칭 병용 처리에 의해서 연근의 갈변이 억제되었음을 확인하였다. 관능 평가와 모든 실험 결과 값의 상관관계 분석에서는 최소 0.84 이상의 상관관계가 있었는데, 특히 $L^*$ 값이 0.93으로 상관관계가 가장 높은 것을 확인 하였다. 따라서 본 연구에서 실시한 이미지 분석법은 다양한 컬러모델을 이용하여서 신선편이 연근의 갈변도를 분석하기에 적합한 것으로 판단된다.

• 한국미생물·생명공학회지
• /
• 제26권3호
• /
• pp.221-225
• /
• 1998

Y-CB와 Y-UCB를 음용하였을 때, 장내 비피더스균수, 유산간균수, 대장균수 및 분변의 pH, 수분함량(%) 등 장내환경 변화에 어떤 영향을 미치는지 검토하였다. 시험 대상자는 20～30세의 건강한 성인 남녀 14명으로 하였으며 평상시의 분변 상태를 조사한 다음 요구르트를 하루 2병씩 식후에 음용하도록 하였다. 음용시험의 방법은 Y-CB와 Y-UCB를 2주간 1차 음용 시험후 2주간의 음용 중단 기간을 가졌으며, 다음 제2차 음용시험으로서는 1차 음용시의 공시요구르트를 교차음용 하면서 분변상태를 비교 분석하였다. Y-UCB의 음용중에는 음용전과 비교하여 비피더스균수(p<0.01)와 유산간균수(p<0.05)는 유의성있게 증가하였고, 수분, pH, 대장균수에는 변화가 없었다. Y-CB의 음용은 음용전과 비교하여 비피더스균수는 증가하였고(p<0.01), 대장균수는 감소하였으며(p<0.05), 유산간균수, 수분, pH는 변화가 없었다. 두 개의 공시 요구르트를 음용 전과 비교하면 음용중단 후에도 비피더스균수가 증가하였고, 낮은 pH를 지속하였으며, 유산간균수, 수분, 대장균수의 변화는 없는 것으로 나타났다 결과적으로 유산균발효유의 음용효과(비피더스균수, 유산간균수, 대장균수, pH, 수분함량의 변화측정 ) 비교에 있어서 본 실험에 사용한 Y-CB나 Y-UCB는 어느 것이나 거의 동등한 효과를 나타내었으며 통계적으로 유의 차가 없는 것으로 나타났다(p>0.05).

• Environmental Sciences Bulletin of The Korean Environmental Sciences Society
• /
• 제10권S_3호
• /
• pp.107-111
• /
• 2001

Surface seawater was sampled from 20 stations in Youngil Bay, Korea in November 2000. The samples were analyzed for eight chlorobenzenes(CBs) out of a total of 12 in the congener series using a gas chromatography coupled to a mass spectrometer detector(GC/MSD). The total CB levels varied from 1.3 to 6.1 ng/L with a mean of 4.0 ng/L. Trichlorobenzene groups (sum of 1,3,5-, 1,2,4-, and 1,2,3-trichlorobenzene) were the predominant class among the four congener groups, while tetrachlorobenzenes(sum of 1,2,3,5-, 1,2,4,5-, and 1,2,3,4- tetrachlorobenzene) and pentachlorobenzene showed a low presence. The total CB levels exhibited similar patterns for all the stations. A significant positive correlation was observed between the individual CB compounds in the particulate samples, while the dissolved samples revealed a strong correlation between the heavier molecular weight CBs.