• 제목/요약/키워드: CAT3

검색결과 1,029건 처리시간 0.029초

Heterologous Production of Pediocin PA-1 in Lactobacillus reuteri

  • Eom, Ji-Eun;Moon, Sung-Kwon;Moon, Gi-Seong
    • Journal of Microbiology and Biotechnology
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    • 제20권8호
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    • pp.1215-1218
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    • 2010
  • The recombinant DNA pLR5cat_PSAB, in which pediocin PA-1 structural and immunity genes (pedAB) fused with the promoter and deduced signal sequence of an ${\alpha}$-amylase gene from a bifidobacterial strain were inserted in Escherichia coli-lactobacilli shuttle vector pLR5cat, was transferred to Lactobacillus reuteri KCTC 3679 and the transformant presented bacteriocin activity. The recombinant L. reuteri KCTC 3679 transformed with the shortened pLR5cat(S)_PSAB, where a nonessential region for the lactobacilli replicon was removed, also showed bacteriocin activity. The molecular mass of the secreted pediocin PA-1 from the recombinant bacteria was the same as that of native pediocin PA-1 (~4.6 kDa) from Pediococcus acidilactici K10 on a sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gel. In cocultures with Listeria monocytogenes, the recombinant L. reuteri KCTC 3679 effectively reduced the viable cell count of the pathogenic bacterium by a 3 log scale compared with a control where L. monocytogenes was incubated alone.

혈뇨를 보이는 고양이의 편측성 신장이형성 증례 (Suspected Unilateral Renal Dysplasia in a Cat with Intermittent Hematuria)

  • 한재익;나기정
    • 한국임상수의학회지
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    • 제32권1호
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    • pp.91-93
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    • 2015
  • 6개월령의 중성화 수컷 고양이가 3일전 관찰된 간헐적인 혈뇨로 내원하였다. 신체검사 및 실험실 검사 결과 특이소견이 관찰되지 않았다. 초음파 검사 결과 복부 중간부분에서 $2{\times}2cm$ 크기의 분리된 낭성 병변이 확인되었다. 병변 내부 물질의 세포학적 검사 결과 다량의 콜레스테롤 결정과 대식구 주종의 육아종성 염증반응이 확인되었다. CT검사에서 낭성 병변은 복부 좌측에 위치하였다. 이상의 결과를 종합하여 환자는 편측성(좌측) 신장 이형성 및 콜레스테롤 결정에 의한 낭 내 염증으로 진단하였다.

전신 마취 후 발생한 고양이의 일시적인 급성 시력상실 1례 (Acute Temporary Visual Loss after General Anesthesia in a Cat)

  • 손원균;정보영;권태억;서강문;이인형
    • 한국임상수의학회지
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    • 제26권5호
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    • pp.480-482
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    • 2009
  • A 2-year-old, castrated male, Scottish fold cat was referred to Veterinary Medical Teaching Hospital of Seoul National University (VMTH-SNU) for evaluation of acute bilateral blindness after general anesthesia. For dental prophylaxis in local animal hospital, general anesthesia had been induced with intravenous acepromazine and ketamine, and maintained with isoflurane after intubation. At VMTH-SNU on next day, complete blood count, electrolytes and serum chemistry values were within normal ranges. On neurologic examination, visual placing and postural reactions like as hopping, hemiwalking and wheelborrowing were reduced on right hindlimb. On ophthalmic examination, menace responses were absent on both eyes and pupillary light reflex (PLR) reduced on right eye, but other reflex and fundus were normal. Prednisolone (2 mg/kg sid for 3 days) was administrated orally and tapered. Visual placing was possible on 2nd day, and postural reactions were recovered on 4th day after dental prophylaxis. Based on the process and recovery, this case was considered as postoperative visual loss (POVL) after general anesthesia.

산화적 스트레스상태에서 B16F10 Murine Melanoma 세포의 항산화효소 활성에 대한 흑축추출물의 효과 (Effect of Pharbitidis Seed Extract on the Antioxidant Enzyme Activity in B16F10 Murine Melanoma Cells by Oxidative Stress)

  • 김안근;차은정
    • 약학회지
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    • 제48권1호
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    • pp.93-98
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    • 2004
  • The purpose of this study was to evaluate the effect of pharbitidis seed extract (PE) on antioxidant enzymes. The cytotoxicities of PE were measured by 3- (4,5-dimethlthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay; The change of superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) activity assay were measured. The SOD activities by PE-treated groups were lower than control group's one. In the co-treated with hydrogen peroxide ($H_2O$$_2$) group, SOD activity was higher than $H_2O$$_2$ treated group's activity In the case of GPx, GPx activities were increased in both PE-treated and co-treated with $H_2O$$_2$ group. In the case of CAT $H_2O$$_2$ treated group's activityies were very increased. The CAT activities by PE-treated groups were lower than control group's one, but the activity of co-treated group with H $_2$O$_2$ was higher than that of control group's one. These results suggest that PE has antioxidant activity.

어류의 조직중에 분포하는 알카리성 단백질분해효소의 활성조건 (Alkaline Pretense Activity of the Tissue Extracts from Some Different Kinds of Fish)

  • 남택정;변재형
    • 한국수산과학회지
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    • 제16권2호
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    • pp.147-153
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    • 1983
  • To check the differences of the digestive enzymes by the bait habits and the proteolytic activities of the fissile extracts from the fish, omnivorous filefish (Navodon modestus), carnivorous cat shark (Scilliorhinus tarazame) and bloodsucking hag fish (Eptatretus burgeri) were sampled for this experiment. The activity of crude alkaline protease extracted from the muscle and the internal organs of the samples was determined with casein as substrate. The activity of the proteolytic enzymes showed remarkable differences by the organs of the fish. The optimum condition of the pretenses from the muscle revealed in range of pH 7.8-8.3, at $60-65^{\circ}C$, while those of the enzymes from the internal organs were at about pH 8.2, $45-55^{\circ}C$, but those of hag fish were at about pH 6.7, $45-55^{\circ}C$. The proteolytic activity of the enzyme of alimentary canal in filefish and in hag fish was 57 and 11 times stronger than that of muscle, respectively. The crude enzyme from the alimentary canal of file fish showed the strongest proteolytic activity in samples submitted and that of cat shark was the lowest. The activity of pancreatic alkaline protease in cat shark was 50 fold higher than that of muscle alkaline protease in the fish.

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The uptake of basic amino acids into fibroblasts was enhanced by PCA.

  • Ogasahara, Kazuko;Takino, Yoshinobu;Sakamoto, Kazutami
    • 대한화장품학회:학술대회논문집
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    • 대한화장품학회 2003년도 IFSCC Conference Proceeding Book II
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    • pp.145-148
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    • 2003
  • Previously, we reported that L-PCA enhanced blood circulation by modulating constitutive NO production. It was that L-PCA increased L-Arg uptake into endothelial cell, followed by the enhancement of NO production. Then we recommended the use of L-PCA for cosmetics, not only as humectants but also as enhancer of blood circulation. Since L-Arg is transported into endothelial cells by CAT (cationic amino acid transporter), it is expected that L-PCA also increase the uptake of basic amino acid, L-Lys. In this study, the uptakes of some amino acids into cells were evaluated by using 3H-labelled amino acid. Then we found the tendency that the uptake of L-Lys into endothelial cells was also enhanced by L-PCA. And the evident effect was observed in the epidermal fibroblasts, which had also CAT. Furthermore, it was found that the transportation of the other type of amino acids were not enhanced by L-PCA. That is to say, a famous moisturizer, L-PCA, has some effects on basic amino acid transport into cells.

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분산식 빗물관리시설 적용에 따른 물수지 변화 분석 연구 - CAT을 이용한 수문모델링 결과를 토대로 - (Analysis of a Change in the Water-Balance after Application of Decentralized Rainwater Management Facilities - Based on the Results of the Hydrologic Modeling using the CAT -)

  • 이태구;한영해
    • KIEAE Journal
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    • 제11권2호
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    • pp.59-65
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    • 2011
  • To analyze changes in the water-balance before and after using decentralized rainwater management facilities, this study carried out hydrologic modeling based on data including roof planting, rainwater use, infiltration and detention facilities applied to the sites. The results of the analysis are as follows: First, the total runoff quantity after facility installation was about 24% less than before. In particular, it showed that the surface runoff declined significantly. Second, the analysis of the effects of different decentralized rainwater management facilities revealed that the rooftop planting contributed to about a 3.5 times increase in actual evaporation than before. Third, the analysis of the effect of decentralized management facilities by different rainfall events showed that it turned to have about a 30% decreasing effect after facility installation for a monthly rainfall over 500mm or so and about 50% declining effect for a monthly rainfall about 200mm. As discussed above, the study confirmed that it is important to implement decentralized rainwater management facilities to improve inevitable changes in water-balance arising from development as it would be a significant alternative for sustainable urban development.

Computed tomographic diagnosis of broncholithiasis in a cat

  • Kim, Rakhoon;An, Soyon;Hwang, Gunha;Ryu, Jeongmin;Kim, Minji;Yoon, Jiwon;Noh, Seul Ah;Yu, DoHyeon;Lee, Hee Chun;Hwang, Tae Sung
    • 대한수의학회지
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    • 제62권3호
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    • pp.23.1-23.5
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    • 2022
  • A 5-year-old castrated male domestic shorthair cat was referred for further investigation of pulmonary nodules incidentally detected on thoracic radiographs. Thoracic radiographs identified ill-defined soft tissue opacity nodules with small faint mineral opacity. Thoracic computed tomography (CT) revealed that bronchial dilation and bronchial wall thickening in bilateral cranial and accessory lung lobes. Round to ellipsoid mineralized concretions were found embedded in the intrabronchial soft tissue attenuation material. Based on radiography, CT, and cytology, the patient was tentatively diagnosed as having broncholithiasis with chronic bronchitis. This report described the CT diagnosis of broncholithiasis, which has been rarely reported in a cat.

ITERATIVE PROCESS FOR FINDING FIXED POINTS OF QUASI-NONEXPANSIVE MULTIMAPS IN CAT(0) SPACES

  • Pitchaya Kingkam;Jamnian Nantadilok
    • Korean Journal of Mathematics
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    • 제31권1호
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    • pp.35-48
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    • 2023
  • Let 𝔼 be a CAT(0) space and K be a nonempty closed convex subset of 𝔼. Let T : K → 𝓟(K) be a multimap such that F(T) ≠ ∅ and ℙT(x) = {y ∈ Tx : d(x, y) = d(x, Tx)}. Define sequence {xn} by xn+1 = (1 - α)𝜈n⊕αwn, yn = (1 - β)un⊕βwn, zn = (1-γ)xn⊕γun where α, β, γ ∈ [0; 1]; un ∈ ℙT (xn); 𝜈n ∈ ℙT (yn) and wn ∈ ℙT (zn). (1) If ℙT is quasi-nonexpansive, then it is proved that {xn} converges strongly to a fixed point of T. (2) If a multimap T satisfies Condition(I) and ℙT is quasi-nonexpansive, then {xn} converges strongly to a fixed point of T. (3) Finally, we establish a weak convergence result. Our results extend and unify some of the related results in the literature.

가뭄년도 농업용저수지 유입량 추정 : 나주호를 중심으로 (Estimation of the Inflow into Agricultural Reservoir during Drought Year : Focused on Naju Reservoir)

  • 정민혁;범진아;윤광식
    • 한국수자원학회:학술대회논문집
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    • 한국수자원학회 2020년도 학술발표회
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    • pp.396-396
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    • 2020
  • 기후변화는 여러 수자원 문제를 야기하며, 특히 가뭄으로 인해 농업용 저수지의 물관리는 점점 중요해지고 있다. 여러 둑높이기 농업용 저수지 중 나주호는 수혜면적에 비해 유역면적이 작아 저수율관리에 어려움을 겪고 있다. 실제로 2017년과 2018년 가뭄이 발생함에 따라 저수율이 20 %까지 저하되었으며, 수혜구역내 간이 양수장, 양수 저류 등 가뭄대책이 시행되었다. 그럼에도 불구하고 나주호의 저수율이 회복되지 않아 단수를 실시하는 등 물관리에 어려움을 겪었다. 이 연구에서는 저수지인 나주호를 대상으로 기상자료, 나주호의 저수율, 공급량 자료를 바탕으로 CAT 모형을 적용 가뭄년도의 유입량 변화를 추정하였다. 검보정의 경우 CAT 3.0 버전에 포함된 SCE-UA 최적화기법을 이용하였다. 그 결과, 2008년부터 2019년까지의 평균 연간 유입량은 6,549 만㎥으로 분석되었으며, 평균 연간 유출율은 48 %로 조사되었다. 하지만, 가뭄이 발생하였던 2017년도의 경우 평균 연간 유입량이 2,070 만㎥, 유출율은 27 %로 조사되었다.

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