• The Korean Journal of Physiology and Pharmacology
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• v.7 no.6
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• pp.349-355
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• 2003

We previously shown that LES contraction depends on $M_3$ receptors linked to PTX insensitive $G_q$ protein and activation of PLC. This results in production of $IP_3$, which mediates calcium release, and contraction through a CaM dependent pathway. In the esophagus ACh activates $M_2$ receptors linked to PTX sensitive $G_{i3}$ protein, resulting in activation of PLD, presumably, production of DAG. We investigated the role of PLC isozymes which can be activated by $G_q$ or $G{\beta}$ protein on ACh-induced contraction in LES and esophagus. Immunoblot analysis showed the presence of 3 types of PLC isozymes, $PLC-{\beta}1$, $PLC-{\beta}3$, and $PLC-{\gamma}1$, but not $PLC-{\beta}2$, $PLC-{\beta}4$, $PLC-{\gamma}2$, $PLC-{\delta}1$, and $PLC-{\delta}2$ from both LES and esophageal muscle. ACh produced contraction in a dose dependent manner in LES and esophageal muscle cells obtained by enzymatic digestion with collagenase. $PLC-{\beta}1$ or $PLC-{\beta}3$ antibody incubation reduced contraction in response to ACh in LES but not in esophageal permeabilized cells, but $PLC-{\gamma}1$ antibody incubation did not have an inhibitory effect. The inhibition by $PLC-{\beta}1$ or $PLC-{\beta}3$ antibody on Ach-induced contraction was antibody concentration dependent. The combination with $PLC-{\beta}_1$ and $PLC-{\beta}_3$ antibody completely abolished the contraction, suggesting that $PLC-{\beta}1$ and $PLC-{\beta}3$ have a synergism to inhibit the contraction in LES. $PLC-{\beta}1$, -${\beta}3$ or -${\gamma}1$ antibody did not reduce the contraction of LES cells in response to DAG ($10^{-6}$ M), suggesting that this isozyme of PLC may not activate PKC. When $G_{q/11}$ antibody was incubated, the inhibitory effect of the incubation of PLC ${\beta}3$, but not of PLC ${\beta}_1$ was additive (Fig. 6). In contrast, when $G_{\beta}$ antibody was incubated, the inhibitory effect of the incubation of PLC ${\beta}_1$, but not of PLC ${\beta}_3$ was additive. This data suggest that $G_{q/11}$/11 or $G{\beta}$ may activate cooperatively different PLC isozyme, $PLC{\beta}_1$ or $PLC{\beta}_3$ respectively.

• The Journal of Industrial Distribution & Business
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• v.9 no.12
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• pp.45-53
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• 2018

Purpose - The purpose of the study was to investigate operation system of current convenience store and competing retail store and to find out development of unattended convenience store. Operators can save labor cost and solve labor seeking when opening shop under suitable environment and commerce area, and assure of employee's safety. Customers are allowed to select commodity comfortably without disclosure of face and to shorten standby time at simple store opening, buying and paying and to produce convenient living environment by financing, delivery and goods control. This study examined effect and problems of operation of existing and unattended convenience store from point of view of visual merchandising to find out model of unattended convenience store and to strengthen competitiveness of convenience store business. Research design, data, and methodology - Research procedure of accomplishment of goal of the study was: The study investigated state of distribution business, and role and definition of VMD. First, VMD played an important role of promotion and competitiveness of unattended convenience stores. Second, the author investigated research and cases of unattended convenience stores 14 months, that is to say, from August 15, 2017 to October 15, 2018. Third, the author investigated on-the-spot three of common convenience stores, three of unattended convenience stores and three of retailers one month, that is to say, June 1, 2018 to July 1, 2018. The subject was convenience stores and retailers selling similar type of products at Seoul and metropolitan area. Results - Layout, selling place, brand identity, goods assortment, authentication system, product information cognition and control, buying and payment system, security system, salesmen, promotion and other services should be improved from point of view of merchandising. Structure, furniture, lighting, voice, façade, VP, PP, IP and POP should be developed. Conclusions - Unattended convenience stores saved operating cost and lessened labor and gave visitors convenience and comfortable shopping and made use of resources effectively. Further studies with quantitative analysis shall find out strategic conditions for promotion of unattended convenience stores.

• Journal of the Korean Institute of Landscape Architecture
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• v.41 no.5
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• pp.57-67
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• 2013

The studies of Korean traditional gardens have been a lot of diachronic approaches through ancient documents and on-site investigation. Previous research has focused on the characteristics that are inherent symbolism of the traditional landscape space, such as site characteristics. There are many studies for inner gardens, outer gardens and other influential ranges of gardens of the location characteristics. However, studies on the scale of external gardens were not satisfactory from a quantitative perspective. Unlike private life sphere, quantitative analysis was conducted on the role of a sphere of public community life for outer gardens. Visibility analysis was performed through the existing literature and GIS programs to estimate the magnitude of the outer garden. When it was compared with Min G. H.(1982) research, it is almost the same if it is estimated to focus on Buyoung -bong(芙蓉峯) and Ip-am(立巖). Also, as a result of the fractal structure for a variety of symbols in the garden, fractal dimension in landscape elements is relatively concentrated, unlike in other areas. Thus, the external scale can be a means of cultural property protection out of the crucial perspective for the inner garden. There has been consideration of the cooperation with the visual complexity using the concept of fractal structure as one of the elements of landscape analysis.

• Journal of Korean Academy of Oral and Maxillofacial Radiology
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• v.27 no.1
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• pp.55-71
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• 1997

Digora/sup (R)/ an intraoral digital radiography system utilizing image plate (IP) - has a dynamic range of exposure time which allows it to decrease the patient's exposure time and to increase diagnostic ability through image processing, transmission and storage. The purpose of this study was to evaluate the Digora/sup (R)/ system by assessing the effects of various exposure times on the detectability on the tip of the endodontic file. Examining the root canals of 45 extracted sound premolars, K -files No. 10, 15, and 20 were placed at slightly varying distances from the apex. The teeth were glued onto resin-plaster blocks. Five exposure times varying between 0.01 seconds and 0.25 seconds were used. Four observers were asked to measure the distance between the tip of the file and a reduction of crown portion, and obtained mean errors (subtracting true file length from the measured file length), comparing Digora/sup (R)/ monitors with E-plus films, which were both obtained under the same geometrical positions. The results were as follows : 1. Comparing E-plus film with Digora/sup (R)/ at 0.01 seconds, the mean errors in E-plus film showed -4.453 nun, -4.497 nun, and -3.857 nun, while the mean errors in Digora/sup (R)/ showed 0.065 nun, 0.607 nun, and 0.719 mm according to the file groups. Therefore there was a significant difference between E-plus film and Digora/sup (R)/(p<0.05). 2. By comparison of mean errors according to the various exposure times in the Digora/sup (R)/ system, the mean error at 0.01 seconds was significantly lower than that at 0.12 and 0.25 seconds in the No. 10 file group(p<0.05). And the standard deviation was the highest at 0.01 seconds. 3. Comparing E-plus film at 0.25 seconds with the Digora/sup (R)/ system, the mean errors showed a significant difference between E-plus film at 0.25 seconds and the Digora/sup (R)/ system at 0.25 seconds in No. 10 and 20 file groups(p<0.05). 4. Comparing E -plus film at 0.25 seconds with other exposure times, the mean errors showed a significant difference between E-plus film at 0.25 seconds and E-plus film at 0 .. 01 and 0.03 seconds in 10 file group(p<0.05). In the No. 15 and 20 file groups, there was a significant difference between E-plus film at 0.25 seconds and E-plus film at 0.01 seconds(p<0.05). In conclusion, Digora/sup (R)/ was better than E-plus film in detectability on the tip of the file at the exposure time of 0.01 seconds in all file groups. And we concluded that Digora/sup (R)/ can shorten exposure times up to 4％ of 0.25 seconds (0.01 sec), which is adequate exposure time for premolar in E-plus film using No. 15 and 20 files.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.46 no.9
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• pp.39-45
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• 2009

When Mobile IPv6 is deployed in commercial network, a mobile node needs AAA services for an authentication, authorization and accounting. AAA and Mobile IPv6 are protocols which are operated independently. Then schemes which merge these protocols have been emerged. These schemes can enable a mobile node to establish a security association between the mobile node and a home agent and to perform a binding update for the home agent using AAA authentication request. But these schemes introduce many signal messages and long handover latency during the handover, since Route Optimization mode for Mobile Ipv6 is performed using Return Routability procedure. To solve this problem, we propose a scheme for Route Optimization mode that the home agent performs the binding update for a correspondent node via the AAA infrastructure between the home agent and the correspondent node instead of Return Routability procedure. For performance evaluation, we analyze signal message transmission costs and handover latencies during handover. We show performance improvement of the proposed scheme which reduces handover latency as 61% compared with the existing scheme.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.30 no.10A
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• pp.886-895
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• 2005

As the networked media technology have been grown in recent, there have been many research works to deliver high-quality video such as HDV and HDTV over the Internet. To realize high-quality media service over the Internet, however, the network adaptive streaming scheme is required to adopt to the dynamic fluctuation of underlying networks. In this paper, we design and implement the network-adaptive HD(high definition) MPEG-2 streaming system employing the frame-based prioritized packetization. Delivered video is inputted from the JVC HDV camera to the streaming sewer in real-time. It has a bit-rate of 19.2 Mbps and is multiplexed to the MPEG-2 TS (MPEG-2 MP@HL). For the monitoring of network status, the packet loss rate and the average jitter are measured by using parsing of RTP packet header in the streaming client and they are sent to the streaming server periodically The network adaptation manager in the streaming server estimates the current network status from feedback packets and adaptively adjusts the sending rate by frame dropping. For this, we propose the real-time parsing and the frame-based prioritized packetization of the TS packet. The proposed system is implemented in software and evaluated over the LAN testbed. The experimental results show that the proposed system can enhance the end-to-end QoS of HD video streaming over the best-effort network.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.42 no.2 s.332
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• pp.23-30
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• 2005

This paper describes a DLL(delay locked loop)-based multi-clock generator having the lower active stand-by power as well as a fast relocking after re-activating the DLL. for low power and high speed VLSI chip. It enables a frequency multiplication using frequency multiplier scheme and produces output clocks with 50:50 duty-ratio regardless of the duty-ratio of system clock. Also, digital control scheme using DAC enables a fast relocking operation after exiting a standby-mode of the clock system which was obtained by storing analog locking information as digital codes in a register block. Also, for a clock multiplication, it has a feed-forward duty correction scheme using multiphase and phase mixing corrects a duty-error of system clock without requiring additional time. In this paper, the proposed DLL-based multi-clock generator can provides a synchronous clock to an external clock for I/O data communications and multiple clocks of slow and high speed operations for various IPs. The proposed DLL-based multi-clock generator was designed by the area of $1796{\mu}m\times654{\mu}m$ using $0.35-{\mu}m$ CMOS process and has $75MHz\~550MHz$ lock-range and maximum multiplication frequency of 800 MHz below 20psec static skew at 2.3v supply voltage.

• Journal of the Korean School Mathematics Society
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• v.14 no.4
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• pp.423-442
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• 2011

The purpose of this study was to examine the effects of tasks setting for mathematical modelling in the complex real situations. The tasks setting(MMa, MeA) in mathematical modelling was so important that we can't ignore its effects to develop meaning and integrate mathematical ideas. The experimental setting were two groups ($N_1=103$, $N_2=103$) at public high school and non-experimental setting was one group($N_3=103$). In mathematical achievement, we found meaningful improvement for MeA group on modelling tasks, but no meaningful effect on information processing tasks. The statistical method used was ACONOVA analysis. Beside their achievement, we were much concerned about their modelling approach that TSG21 had suggested in Category "Educational & cognitive Midelling". Subjects who involved in experimental works showed very interesting approach as Exploration, analysis in some situation ${\Rightarrow}$ Math. questions ${\Rightarrow}$ Setting models ${\Rightarrow}$ Problem solution ${\Rightarrow}$ Extension, generalization, but MeA group spent a lot of time on step: Exploration, analysis and MMa group on step, Setting models. Both groups integrated actively many heuristics that schoenfeld defined. Specially, Drawing and Modified Simple Strategy were the most powerful on approach step 1,2,3. It was very encouraging that those experimental setting was improved positively more than the non-experimental setting on mathematical belief and interest. In our school system, teaching math. modelling could be a answer about what kind of educational action or environment we should provide for them. That is, mathematical learning.

• Journal of KSNVE
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• v.8 no.5
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• pp.964-973
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• 1998

In this paper we propose a levitation and imbalance compensation controller design methodology of magnetic bearing system. In order to achieve levitation and elimination of unbalance vibartion in some operation speed we use the discrete-time Q-parameterization control. When rotor speed p = 0 there are no rotor unbalance, with frequency equals to the rotational speed. So in order to make levitatiom we choose the Q-parameterization controller free parameter Q such that the controller has poles on the unit circle at z = 1. However, when rotor speed p $\neq$ 0 there exist sinusoidal disturbance forces, with frequency equals to the rotational speed. So in order to achieve asymptotic rejection of these disturbance forces, the Q-parameterization controller free parameter Q is chosen such that the controller has poles on the unit circle at z = $exp^{ipTs}$ for a certain speed of rotation p ( $T_s$ is the sampling period). First, we introduce the experimental setup employed in this research. Second, we give a mathematical model for the magnetic bearing in difference equation form. Third, we explain the proposed discrete-time Q-parameterization controller design methodology. The controller free parameter Q is assumed to be a proper stable transfer function. Fourth, we show that the controller free parameter which satisfies the design objectives can be obtained by simply solving a set of linear equations rather than solving a complicated optimization problem. Finally, several simulation and experimental results are obtained to evaluate the proposed controller. The results obtained show the effectiveness of the proposed controller in eliminating the unbalance vibrations at the design speed of rotation.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.9
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• pp.3667-3671
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• 2015

Invasion and metastasis is the major cause of tumor recurrence, difficulty for cure and low survival rate. Excavating key transcription factors, which can regulate tumor invasion and metastasis, are crucial to the development of therapeutic strategies for cancers. PU.1 is a master hematopoietic transcription factor and a vital regulator in life. Here, we report that, compared to adjacent non-cancerous tissues, expression of PU.1 mRNA in metastatic hepatocellular carcinoma (HCC), but not primary HCC, was significantly down-regulated. In addition, levels of PU.1 mRNA in metastatic hepatoma cell lines MHCC97L and MHCC97H were much lower than in non-metastatic Hep3B cells. Transwell invasion assays after PU.1 siRNA transfection showed that the invasion of hepatoma cell lines was increased markedly by PU.1 knockdown. Oppositely, overexpression of PU.1 suppressed the invasion of these cells. However, knockdown and overexpression of PU.1 did not influence proliferation. Finally, we tried to explore the potential mechanism of PU.1 suppressing hepatoma cell invasion. ChIP-qPCR analysis showed that PU.1 exhibited a high binding capacity with miR-615-5p promoter sequence. Overexpression of PU.1 caused a dramatic increase of pri-, pre- and mature miR-615-5p, as well as a marked decrease of miR-615-5p target gene IGF2. These data indicate that PU.1 inhibits invasion of human HCC through promoting miR-615-5p and suppressing IGF2. These findings improve our understanding of PU.1 regulatory roles and provided a potential target for metastatic HCC diagnosis and therapy.