• The Korean Journal of Mycology
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• v.23 no.3 s.74
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• pp.202-208
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• 1995

This study describes the effects of several components on PCR amplification used for RAPD. We used different concentrations of reaction components to obtaine discrete and reproducible PCR products from Pleurotus cornucopiae. The optimum concentrations of reaction components were found to be 80 ng of template DNA, 30 pmole of 10-mer primer, $200\;{\mu}M$ dNTP, 2mM $MgCl_2$, 50 mM KCl, 10 mM Tris-HCl(pH 9.0), 0.1% Triton X-100, 1.5 unit of Taq DNA polymerase (promega) in $50\;{\mu}l$ reaction volume. The optimum annealing temperature was $35^{\circ}C$. These results proved to be valuable for characterization of Pleurotus spp.

• The KIPS Transactions:PartC
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• v.11C no.4
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• pp.429-438
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• 2004

This Paper Presents the control structure for incoming traffic from arbitrary node to Provide admission control in policy-based W network management structure using fuzzy logic control approach. The proposed control structure uses scheme for deciding network resource allocation depending on requirements predefined-policies and network states. The proposed scheme enhances policy adapting methods of existing binary methods, and can use resource of network more effectively to provide adaptive admission control, according to the unpredictable network states for predefined QoS policies. Simulation results show that the proposed controller improves the ratio of packet rejection up to 26%, because it Performs the soft adaption based on the network states instead of accept/reject action in conventional CAC(Connection Admission Controller).

• Computers and Concrete
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• v.8 no.2
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• pp.193-206
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• 2011

The use of Carbon Fiber Reinforced Polymers (CFRP) to strengthen reinforced concrete beams under bending and shear has gained rapid growth in recent years. The performance of shear strengthened beams with externally bonded CFRP laminate or fabric strips is raising many concerns when the beam is loaded under cyclic loading. Such concerns warrant experimental, analytical and numerical investigation of such beams under cyclic loading. To date, limited investigations have been carried out to address this concern. This paper presents a numerical investigation by developing a nonlinear finite element (FE) model to study the response of a cantilever reinforced concrete T-beam strengthened in shear with side bonded CFRP fabric strips and subjected to cyclic loading. A detailed 3D nonlinear finite element model that takes into account the orthotropic nature of the polymer's fibers is developed. In order to simulate the bond between the CFRP sheets and concrete, a layer having the material properties of the adhesive epoxy resin is introduced in the model as an interface between the CFRP sheets and concrete surface. Appropriate numerical modeling strategies were used and the response envelope and the load-displacement hysteresis loops of the FE model were compared with the experimental response at all stages of the cyclic loading. It is observed that the responses of the FE beam model are in good agreement with those of the experimental test. A parametric study was conducted using the validated FE model to investigate the effect of spacing between CFRP sheets, number of CFRP layers, and fiber orientation on the overall performance of the T-beam. It is concluded that successful FE modeling provides a practical and economical tool to investigate the behavior of such strengthened beams when subjected to cyclic loading.

• Computers and Concrete
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• v.8 no.2
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• pp.125-142
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• 2011

In the present paper, a numerical simulation method based on mesoscopic composite structure of concrete, the truss network model, is developed to evaluate the diffusivity of concrete in order to account for the microstructure of concrete, the binding effect of chloride ions and the chloride concentration dependence. In the model, concrete is described as a three-phase composite, consisting of mortar, coarse aggregates and the interfacial transition zones (ITZs) between them. The advantage of the current model is that it can easily represent the movement of mass (e.g. water or chloride ions) through ITZs or the potential cracks within concrete. An analytical method to estimate the chloride diffusivity of mortar and ITZ, which are both treated as homogenious materials in the model, is introduced in terms of water-to-cement ratio (w/c) and sand volume fraction. Using the newly developed approaches, the effect of cracking of concrete on chloride diffusion is reflected by means of the similar process as that in the test. The results of calculation give close match with experimental observations. Furthermore, with consideration of the binding capacity of chloride ions to cement paste and the concentration dependence for diffusivity, the one-dimensional nonlinear diffusion equation is established, as well as its finite difference form in terms of the truss network model. A series of numerical analysises performed on the model find that the chloride diffusion is substantially influenced by the binding capacity and concentration dependence, which is same as that revealed in some experimental investigations. This indicates the necessity to take into account the binding capacity and chloride concentration dependence in the durability analysis and service life prediction of concrete structures.

• Computers and Concrete
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• v.11 no.2
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• pp.93-104
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• 2013

The use of rock wool waste, an industrial by-product, in cement-based composites has positive effects on the environment because it reduces the problems associated rock wool disposal. The experiments in this study tested cement-based composites using various rock wool waste contents (10, 20, 30 and 40% by weight of cement) as a partial replacement for Portland cement in mortars. The pozzolanic strength activity test, flow test, compressive strength test, dry shrinkage test, absorption test, initial surface absorption test and scanning electron microscope observations were conducted to evaluate the properties of cement-based composites. Test results demonstrate that the pozzolanic strength activity index for rock wool waste specimens is 103% after 91 days. The inclusion of rock wool waste in cement-based composites decreases its dry shrinkage and initial surface absorption, and increases its compressive strength. These improved properties are the result of the dense structure achieved by the filling effect and pozzolanic reactions of the rock wool waste. The addition of 30% and 10% rock wool wastes to cement is the optimal amount based on the results of compressive strength and initial surface absorption for a w/cm of 0.35 and 0.55, respectively. Therefore, it is feasible to utilize rock wool waste as a partial replacement of cement in cement-based composites.

• Computers and Concrete
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• v.17 no.3
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• pp.423-433
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• 2016

After the industrial of steelmaking by-products are processed properly, they can be used in civil engineering, not only as a substitute for natural resources and to reduce costs, but also to provide environmental protection. This study used different amounts (10%, 20%, 30%, 40%,and 50%) of desulphurization slag to replace natural fine aggregates in ready-mixed soil materials, and tested the physical and fresh properties (slump, slump flow, tube flow, initial setting time, and bleeding) and hardened properties (compressive strength, ball drop, ultrasonic pulse velocity) of the materials. The variations between the performances of the materials with different mix proportions were discussed. When desulphurization slag is used in RMSM, the workability can be enhanced obviously significantly. When the replacement of desulphurization slag is 50%, the slump flow is increased by 110mm compared with the control group, and the initial setting time increases as the replacement increases, because of bleeding. When the replacement is 10% and 20%, the compressive strength at various ages is higher than that of the control group. When the replacement is 10%, the compressive strength at 7 days is higher than that of the control group by 60%, and the ultrasonic pulse velocity is proportional to the compressive strength, which increases with age and decrease as the replacement increases. An appropriate replacement can effectively accelerate construction, and allow projects to be finished ahead of schedule; therefore, an appropriate replacement, is applicable for ready-mixed soil materials.

• Computers and Concrete
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• v.26 no.4
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• pp.343-350
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• 2020

Concrete-filled steel tubular (CFST) members have been widely used in engineering, and their tube diameters have become larger and larger. But there is no research on the thermal field of large-diameter CFST structure. These studies focused on the thermal field of the large-diameter CFST structure under solar radiation. The environmental factors and the actual placement position were considered, and the finite element model (FEM) of the thermal field of CFST members under solar radiation (SR) was established. Then the FEM was verified by practical experiments. The most unfavorable temperature gradient model in the cross-section was proposed. The testing results showed that the temperature field of the large-diameter CFST member section was non-linearly distributed due to the influence of SR. The temperature field results of CFST members with different pipe diameters indicated that the larger the core concrete diameter was, the slower the central temperature changed, and there was a significant temperature difference between the center and the boundary. Based on the numerical model, the most unfavorable temperature gradient model in the section was proposed. The model showed that the temperature difference around the center of the circle is small, and the boundary temperature difference is significant. The maximum temperature difference is 15.22℃, which appeared in the southern boundary area of the specimen. Therefore, it is necessary to consider the influence of SR on the thermal field of the member for large-diameter CFST members in actual engineering, which causes a large temperature gradient in the member.

• Computers and Concrete
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• v.26 no.4
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• pp.327-342
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• 2020

The objective of this study is to manufacture environmentally-friendly synthetic lightweight aggregates that may be used in the structural lightweight concrete production. The cold-bonding pelletization process has been used in the agglomeration of the pozzolanic materials to achieve these synthetic lightweight aggregates. In this context, it was aimed to recycle the waste fly ash by employing it in the manufacturing process as the major cementitious component. According to the well-known facts reported in the literature, it is stated that the main disadvantage of the synthetic lightweight aggregate produced by applying the cold-bonding pelletization technique to the pozzolanic materials is that it has a lower strength in comparison with the natural aggregate. Therefore, in this study, the metakaolin made of high purity kaolin and calcined kaolin obtained from impure kaolin have been employed at particular contents in the synthetic lightweight aggregate manufacturing as a cementitious material to enhance the particle crushing strength. Additionally, to propose a curing condition for practical attempts, different curing conditions were designated and their influences on the characteristics of the synthetic lightweight aggregates were investigated. Three substantial features of the aggregates, specific gravity, water absorption capacity, and particle crushing strength, were measured at the end of 28-day adopted curing conditions. Observed that the incorporation of thermally treated kaolin significantly influenced the crushing strength and water absorption of the aggregates. The statistical evaluation indicated that the investigated properties of the synthetic lightweight aggregate were affected by the thermally treated kaolin content more than the kaoline type and curing regime. Utilizing the thermally treated kaolin in the synthetic aggregate manufacturing lead to a more than 40% increase in the crushing strength of the pellets in all curing regimes. Moreover, two numerical formulations having high estimation capacity have been developed to predict the crushing strength of such types of aggregates by using soft-computing techniques: gene expression programming and artificial neural networks. The R-squared values, indicating the estimation performance of the models, of approximately 0.97 and 0.98 were achieved for the numerical formulations generated by using gene expression programming and artificial neural networks techniques, respectively.

• Computers and Concrete
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• v.26 no.4
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• pp.367-375
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• 2020

Today, the use of special technologies in the admixture of concrete has made tremendous progress, but the problem that has always existed in the construction of concrete members is the brittleness and lack of loading bearing after cracking, which leads to reduced strength and energy absorption. One of the best ways to fix this is to reinforce the concrete with steel fibers. Steel fibers also control cracks due to dry shrinkage, reduce structural crack width, and improve impact resistance. In this study, recycled steel fibers from worn tires have been used in the manufacture of concrete samples, the secondary benefits of which are the reduction of environmental pollution. One of the disadvantages of steel fiber reinforced concrete is the corrosion of steel fibers and their deterioration in harsh environments such as coastal areas. Corrosion caused by chlorine ions in metal fibers causes deterioration and early decommissioning of structures in corrosive environments. In this study, the effect of the dosage of steel fibers (dosages of 15, 30, and 45 kg of fibers per cubic meter of concrete) and aspect ratio of fibers (aspect ratio of 25 and 50) on compressive and flexural strength of concrete samples are investigated. In the following, the effect of fiber corrosion on the results of the mechanical properties of concrete samples is examined. The results show that the increase in fiber causes a relative increase in compressive strength, and a significant increase in flexural strength, and corrosion of steel fibers without reducing workability reduces compressive strength and flexural strength by up to 6 to 11%, respectively.

• Computers and Concrete
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• v.26 no.4
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• pp.309-316
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• 2020

The application of multi-variable adaptive regression spline (MARS) in predicting he long-term compressive strength of a concrete with various admixtures has been investigated in this study. The compressive strength of concrete specimens, which were made based on 24 different mix designs using various mineral and chemical admixtures in different curing ages have been obtained. First, The values of fly ash (FA), micro-silica (MS), water-reducing admixture (WRA), coarse and fine aggregates, cement, water, age of samples and compressive strength were defined as inputs to the model, and MARS analysis was used to model the compressive strength of concrete and to evaluate the most important parameters affecting the estimation of compressive strength of the concrete. Next, the proposed equation by the MARS method using particle swarm optimization (PSO) algorithm has been optimized to have more efficient equation from the economical point of view. The proposed model in this study predicted the compressive strength of the concrete with various admixtures with a correlation coefficient of R=0.958 rather than the measured compressive strengths within the laboratory. The final model reduced the production cost and provided compressive strength by reducing the WRA and increasing the FA and curing days, simultaneously. It was also found that due to the use of the liquid membrane-forming compounds (LMFC) for its lower cost than water spraying method (SWM) and also for the longer operating time of the LMFC having positive mechanical effects on the final concrete, the final product had lower cost and better mechanical properties.