• Title/Summary/Keyword: CA1

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Prevention of Quality Deterioration in Concentrated Strawberry Puree by Employing pH Regulators during Heat Treatment (pH 조절제를 이용한 농축 딸기 퓌레 열처리 시 품질저하 억제)

  • Lee, In-Gyeong;Min, Seo-Cheol;Kim, Hee-Sun;Han, Gwi-Jung;Kim, Myung-Hwan
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.45 no.10
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    • pp.1518-1524
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    • 2016
  • This study was performed to minimize quality degradation of concentrated strawberry puree after $90^{\circ}C$ for 5 min by heat treatment with citric acid (CA) and acidic sodium metaphosphate (ASM) as pH modifiers. The highest color value was 1% CA+1% ASM (13.027), followed by 1% CA (9.539) and control (6.905). Anthocyanin contents were also 1% CA+1% ASM (3.049 mg/100 g), 1% CA (1.140 mg/100 g), and control (0.757 mg/100 g) in sequence. The DPPH radical scavenging activities of control, 1% CA, and 1% CA+1% ASM were 58.148, 72.638, and 83.736%, respectively. The color values, anthocyanin contents, and DPPH radical scavenging activities were significantly different at P<0.05 between control and 1% CA+1% ASM treatment. For the results of overall preference for hedonic test, there was no significant difference at P>0.05 among the three samples. During heat treatment, quality degradation of concentrated strawberry puree was reduced by 1% CA+1% ASM treatment, which is expected for new acidulants.

Synthesis and luminescence properties of $Sr_{1-x}Ca_xGa_2S_4:Ce,Na$ phosphors ($Sr_{1-x}Ca_xGa_2S_4:Ce,Na$ 형광체의 합성과 발광 특성)

  • Sung, Hye-Jin;Huh, Young-Duk
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.16 no.6
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    • pp.267-272
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    • 2006
  • A series of $Sr_{1-x}Ca_xGa_2S_4:Ce,Na$ phosphors have been synthesized by solid-state reaction. The photoluminescence and structural properties of $Sr_{1-x}Ca_xGa_2S_4:Ce,Na$ have been examined. The $Sr_{1-x}Ca_xGa_2S_4:Ce,Na$ phosphors have a strong absorption at 400 nm, which is the emission wavelength of a violet light emitting diode (LED). The emission peaks of $SrGa_2S_4:Ce,Na$are located at 448 nm and 485 nm. The partial replacement of Sr by Ca in $Sr_{1-x}Ca_xGa_2S_4:Ce,Na$ causes a red shift of emission wavelengths. The $Sr_{1-x}Ca_xGa_2S_4:Ce,Na$ can be used as blue emitting phosphors pumped by the violet LED for fabricating the multi-band white LED.

Synthesis of Uniform CA and 90/150 Hybrid CA (Uniform CA와 90/150 Hybrid CA의 합성)

  • Kim, Han-Doo;Cho, Sung-Jin;Choi, Un-Sook;Kwon, Min-Jeong;Kong, Gil-Tak
    • The Journal of the Korea institute of electronic communication sciences
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    • v.11 no.3
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    • pp.293-302
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    • 2016
  • In this paper we analyze the CA formed by combining the uniform 102 CA $\mathbb{C}_u$ and the m-cell 90/150 hybrid CA $\mathbb{C}_h$ whose characteristic polynomial is $(x+1)^m$. We analyze cycle structures of complemented group CA derived from $\mathbb{C}_u$ and propose a condition of complemented CA dividing the entire state space into smaller cycles of equal lengths. And we analyze the cycle structure of complemented group CA $\mathbb{C}^{\prime}$ derived from the CA $\mathbb{C}$ formed by combining $\mathbb{C}_u$ and $\mathbb{C}_h$ with complement vector F such that $(T+I)^{q-1}F{\neq}0$ where $(x+1)^q$ is the minimal polynomial of $\mathbb{C}$.

Effects of CellCaSi and Inorganic Additives on Phosphorus Removal in Water (규산질다공체와 무기첨가물의 수중 인 제거 효과)

  • Park, Myung-Hwan;Han, Myung-Soo;Lee, Seog-June;Ahn, Chi-Yong;Yoon, Byung-Dae;Oh, Hee-Mock
    • Korean Journal of Ecology and Environment
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    • v.35 no.3 s.99
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    • pp.213-219
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    • 2002
  • The CellCaSi, a porous silicate material, was tested for the removal of phosphorus (P as phosphate) in water. The effect of the CellCaSi was investigated on the basis of both particle size (under 1,2, and 4 mm) and added amount (0, 1, 2.5, 5, and 10 g/1) of the CellCaSi. The removal efficiency of phosphorus was highest with a particle size of under 1 mm and also increased with an increasing amount of the CellCaSi. The pH change showed little effect on the phosphorus removal of the CellCaSi. The calcium ion was eluted from the CellCaSi into the water, while the aluminium and iron were not. The eluted calcium ion was combined with dissolved phosphorus and then precipitated. The highest removal efficiency of phosphorus was obtained by the combined addition of the CellCaSi, calcium chloride, and ferric chloride. That is, the phosphorus concentrations of 0.10 and 1.0 mg/1 decreased to 0.03 and 0.47 mg/l by the addition of the CellCaSi (1 g/l), calcium ion (30 mg/l), and ferric ion (1 mg/l) at day 8 after treatment. The water qualities at the end of the experiment were as follows: pH was 8.1 and conductivity was 318 ${\mu}$S/cm (a registered maximum conductivity of 500${\mu}$S/cm for raw and potable wafers).

On the Construction of the 90/150 State Transition Matrix Corresponding to the Trinomial x2n-1 + x + 1 (3항 다항식 x2n-1 + x + 1에 대응하는 90/150 상태전이행렬의 구성)

  • Kim, Han-Doo;Cho, Sung-Jin;Choi, Un-Sook
    • The Journal of the Korea institute of electronic communication sciences
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    • v.13 no.2
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    • pp.383-390
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    • 2018
  • Since cellular automata(CA) is superior to LFSR in randomness, it is applied as an alternative of LFSR in various fields. However, constructing CA corresponding to a given polynomial is more difficult than LFSR. Cattell et al. and Cho et al. showed that irreducible polynomials are CA-polynomials. And Cho et al. and Sabater et al. gave a synthesis method of 90/150 CA corresponding to the power of an irreducible polynomial, which is applicable as a shrinking generator. Swan characterizes the parity of the number of irreducible factors of a trinomial over the finite field GF(2). These polynomials are of practical importance when implementing finite field extensions. In this paper, we show that the trinomial $x^{2^n-1}+X+1$ ($n{\geq}2$) are CA-polynomials. Also the trinomial $x^{2^a(2^n-1)}+x^{2^a}+1$ ($n{\geq}2$, $a{\geq}0$) are CA-polynomials.

DTNB oxidation effects on T-type $Ca^{2+}$ channel isoforms

  • Lee, Sang-Soo;Kang, Ho-Won;Park, Jin-Yong;Lee, Jung-Ha
    • Animal cells and systems
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    • v.15 no.2
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    • pp.131-138
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    • 2011
  • Redox regulation is one of the ubiquitous mechanisms to modulate ion channels. We here investigated how 5,5'-dithio-bis (2-nitrobenzoic acid), a cysteine specific oxidizing reagent, modulates $Ca_v3.1$ and $Ca_v3.2$ T-type $Ca^{2+}$ channels expressed in Xenopus oocytes. Application of the reagent inhibited $Ca_v3.1$ and $Ca_v3.2$ currents in a dose-dependent manner. The oxidizing reagent (1 mM) reduced the peak amplitude of $Ca_v3.1$ and $Ca_v3.2$ currents by ~50% over 2-3 minutes and the decreased currents were fully recovered upon washout of it. The reagent slowed the activation and inactivation kinetics of $Ca_v3.1$, $Ca_v3.2$, and $Ca_v3.3$ channel currents. Notably, the reagent positively shifted both activation and steady-state inactivation curves of $Ca_v3.1$, while it did not those of $Ca_v3.2$. Utilizing chimeric channels from $Ca_v3.1$ and $Ca_v3.2$, we localized the domains III and IV of $Ca_v3.1$ responsible for the positive shifts of channel activation and steady-state inactivation. These findings provide hints relevant to the electrophysiological and molecular mechanisms accounting for the oxidative regulation of T-type channels.

Mechanism of $Ca^{2+}$ Regulation in Osteoblast-like Cells (골아세포내 $Ca^{2+}$ 활성도의 조절기전)

  • Park, Mi-Jung
    • Journal of Korean Biological Nursing Science
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    • v.1 no.1
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    • pp.25-41
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    • 1999
  • Physiological activity of osteoblast including bone formation is known to be closely related to the increase of intracellular $Ca^{2+}$ activity($[Ca^{2+}]_i$) in osteoblast. $Ca^{2+}$ is an important intracellular messenger in diverse cellular functions, and regulation of its level is mediated by the transmembrane $Ca^{2+}$ movement via $Ca^{2+}$ channels, $Na^+-Ca^{2+}$ exchange, and by intracellular $Ca^{2+}$ movement through the intracellular stores. The purpose of this study is to investigate how the intracellular $Ca^{2+}$ is regulated in osteoblast-like cells(OLCs) by measuring $Ca^{2+}$ activity with cell imaging technique. OLCs were isolated from femur and tibia of neonatal rats, and cultured for 7 days. Cultured OLCs were loaded with a $Ca^{2+}$-sensitive fluorescent dye, Fura-2, and fluorescence images were monitored with a cooled CCD camera. The images were processed and analyzed with an image analyzing software. The results were as follows. (1) $[Ca^{2+}]_i$ of OLC decreased as the $Ca^{2+}$ concentration in the superfusing Tyrode solution was lowered. When $Na^+$ concentration in the superfusing solution was decreased, $[Ca^{2+}]_i$ increased.. These suggest that $Ca^{2+}$ flux occurs via the $Na^+-Ca^{2+}$ exchange mechanism. (2) When $Na^+$ in the superfusing solution was removed. a transient $Ca^{2+}$, increase($Ca^{2+}$ spike) was occasionally observed. However, $Ca^{2+}$ spike was not observed after adding 1 ${\mu}M$ thapsigargin. This implies that the generation of $Ca^{2+}$ spike is mediated by the release of $Ca^{2+}$ from endoplasmic reticulum(ER). (3) As the $Ca^{2+}$ concentration in the superfusing solution was raised, the frequency of 0mM $Na^+$-induced $Ca^{2+}$ spike increased, suggesting that $Ca^{2+}$-induced $Ca^{2+}$ release(CICR) mechanism exists. (4) After $[Ca^{2+}]_i$ was decreased with the superfusion of $Ca^{2+}$-free solution containing thapsigargin, the recovery of $[Ca^{2+}]_i$ with reperfusion of 2.5mM $Ca^{2+}$ solution transiently exceeded the control level, suggesting that the depletion of $Ca^{2+}$ in ER induces $Ca^{2+}$ influx from extracellular medium via store-operated $Ca^{2+}$ influx(SOCI) mechanism. (5) $[Ca^{2+}]_i$ was not affected by the superfusion of 25mM $K^+$ Tyrode solution. These results suggest that intracellular $Ca^{2+}$ activity in osteoblast is regulated by transmembrane $Ca^{2+}$ flux via $Na^+-Ca^{2+}$ exchange, $Ca^{2+}$ release from the internal store (ER) via $Ca^{2+}$-induced $Ca^{2+}$ release, and store-operated $Ca^{2+}$ influx across the cell membrane.

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Ca2+/calmodulin-dependent regulation of polycystic kidney disease 2-like-1 by binding at C-terminal domain

  • Baik, Julia Young;Park, Eunice Yon June;So, Insuk
    • The Korean Journal of Physiology and Pharmacology
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    • v.24 no.3
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    • pp.277-286
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    • 2020
  • Polycystic kidney disease 2-like-1 (PKD2L1), also known as polycystin-L or TRPP3, is a non-selective cation channel that regulates intracellular calcium concentration. Calmodulin (CaM) is a calcium binding protein, consisting of N-lobe and C-lobe with two calcium binding EF-hands in each lobe. In previous study, we confirmed that CaM is associated with desensitization of PKD2L1 and that CaM N-lobe and PKD2L1 EF-hand specifically are involved. However, the CaM-binding domain (CaMBD) and its inhibitory mechanism of PKD2L1 have not been identified. In order to identify CaM-binding anchor residue of PKD2L1, single mutants of putative CaMBD and EF-hand deletion mutants were generated. The current changes of the mutants were recorded with whole-cell patch clamp. The calmidazolium (CMZ), a calmodulin inhibitor, was used under different concentrations of intracellular. Among the mutants that showed similar or higher basal currents with that of the PKD2L1 wild type, L593A showed little change in current induced by CMZ. Co-expression of L593A with CaM attenuated the inhibitory effect of PKD2L1 by CaM. In the previous study it was inferred that CaM C-lobe inhibits channels by binding to PKD2L1 at 16 nM calcium concentration and CaM N-lobe at 100 nM. Based on the results at 16 nM calcium concentration condition, this study suggests that CaM C-lobe binds to Leu-593, which can be a CaM C-lobe anchor residue, to regulate channel activity. Taken together, our results provide a model for the regulation of PKD2L1 channel activity by CaM.

Influence of $Ca^{++}$ Treatment on Growth and Mineral Elements of Cool-Season Turfgrass Species ($Ca^{++}$ 처리가 한지형 잔디의 생육 및 무기성분 함량에 미치는 영향)

  • Kim, Seong-Kyoo;Kang, Hoon;So, In-Sup
    • Asian Journal of Turfgrass Science
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    • v.16 no.1
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    • pp.1-10
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    • 2002
  • The influence of calcium on the growth of creeping bentgrass 'Penn-Al', perennial ryegrass 'Palmer II', Kentuckuy bluegrass 'Nassou' and tall fescue 'Boonsai 2000' in greenhouse was investigated. Creeping bentgrass 'Penn-A1', Kentucky bluegrass 'Nassou' and tall fescue 'Boonsai 2000' at Ca 4.0 me/L, and perennial ryegrass 'Palmer II' at Ca 2.0 me/L showed the best shoot growth. Creeping bentgrass 'Penn-A1', perennial ryegrass 'Palmer II' and Kentucky bluegrass 'Nassou' at Ca 1.0 me/L, and tall fescue 'Boonsai 2000' at Ca 4.0 me/L showed the best root growth, and there was little or no difference between different Ca concentrations. Creeping bentgrass 'Penn-Al' and Kentucky bluegrass 'Nassou' at Ca 4.0 mea, and perennial ryegrass 'Palmer II' at Ca 1.0 me/L had the highest number of tillers, and tall fescue 'Boonsai 2000' at Ca 4.0 me/L had the highest, but there was no difference between different Ca concentrations. As application rate of Ca concentration became higher, the concent of Ca in plant tissue increased, while the content of Mg in plant tissue decreased, and the content of Fe in plant tissue increased to Ca 4.0 me/L. The Ca treatment had not effect on N, p, K, and Zn of tissue content. The wick pot applied will be to research of plant nutrition in future because utilization of wick pot has an excellent precision and convenience.

Fruit Quality Characteristics of 'Fuji' Apple Fruits in Response to Air Storage after CA Storage (Controlled Atmosphere (CA) 환경설정 해제 후 저온저장이 '후지' 과실의 품질에 미치는 영향)

  • Kweon, Hun-Joong;Choi, Dong Geun;Lee, Jinwook;Kang, In-Kyu
    • Horticultural Science & Technology
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    • v.32 no.2
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    • pp.178-183
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    • 2014
  • This study aimed to evaluate the fruit quality characteristics and incidence of flesh browning in response to air storage at $0{\pm}1^{\circ}C$ a fter controlled atmosphere s torage (CA condition: $O_2$ $2.5{\pm}0.5$%, $CO_2$ $1.5{\pm}0.5$%) at $0{\pm}1^{\circ}C$ in 'Fuji' apples (Malus domestica Borkh.). The storage system was performed as followed: air storage for one month, CA storage 4 months + air storage 3 months ( CA 4M + A ir 3M), CA storage 5 m onths + air storage 2 months ( CA 5M + Air 2M) a nd C A storage 6 m onths + air storage 1months (CA 6M + Air 1M), while the control fruits were stored at CA storage for 8 months right after harvest. The incidence of flesh browning ranged from 17.1% to 30.2% during CA storage but not detected under the treatments of CA 4M + Air 3M and CA 5M + Air 2M. The respiration rate was not affected by storage treatments for 6M while the respiration rate was lower in the treatments of CA 4M + Air 3M and CA 5M + Air 2M than the other storage treatments after 7 months. Ethylene production and internal ethylene concentration were lowest in rapid CA storage and increased with a decreasing CA storage duration. Therefore, the results indicate that CA 5M + Air 2M storage treatment should be recommended to maintain the fruit quality and reduce the risk development of flesh browning rather than typical CA storage in 'Fuji' apples.